Agricultural and Biological Sciences, Biotechnology, Molecular Biology, Catalysis
162
Scopus Publications
4756
Scholar Citations
42
Scholar h-index
100
Scholar i10-index
Scopus Publications
Exploring hot-spring metagenomes for the repertoire of carbohydrate-active enzymes and potential bacterial strains for enzyme production Reena Kumari, Nitish Sharma, Kshetrimayum Birla Singh, Sudhir Pratap Singh, Amit Kumar Rai Heliyon, 2026 This study employs a targeted metagenome mining approach to identify carbohydrate-active enzymes (CAZymes) from thermal aquatic environments in the Sikkim Himalaya, followed by functional validation through enzyme screening of bacterial isolates. Metagenomic approach highlighted an abundance of CAZymes in the metagenomic data of Yumthang (3,934), followed by Borong (1,606), Lingdem (1,557), Polok (1096), and Reshi (831). Over 50% of the predicted repertoire was found to be primarily constituted by two classes of CAZyme: glycosyl transferases (GT) (45%) and glycoside hydrolases (GH) (28%). Bacterial isolates were screened to produce high-value biomass transforming enzymes such as xylanase, pectinase, amylase, and β -glucosidase based on the metagenomic data. The enzymes produced from the selected bacterial isolates of Bacillus velezensis PBW5, Bacillus subtilis PBW7, Bacillus haynesii ZNS4, and Bacillus licheniformis PAS3 showed activity at high temperature. B. velezensis PBW5 and strains of B. subtilis were identified as potential producers of xylanase apart from ability to produce other CAZymes. At the same time, B. licheniformis PAS3 and B. haynesii ZNS4 were recorded to be the effective pectinase and amylase producers. The process was developed for submerged and solid-state fermentation (using industrial orange pomace) for simultaneous production of xylanase and pectinase from B. velezensis PBW5 and B . licheniformis PAS3 having activity at high temperature range (50-80°). This study has been pivotal in exploring the enzymatic repertoire of microorganisms from untapped ecological niches of Sikkim and identifying novel strains for the biomass transformation into value-added products.
Novel Glutamate Decarboxylase from Ethnic Food Metagenome: A Potential Biocatalyst to Produce a Nonproteinaceous Amino Acid, γ-Aminobutyric Acid Shantanu B. Sathaye, Nitish Sharma, Jayeeta Bhaumik, Dinabandhu Sahoo, Amit Kumar Rai, Sudhir Pratap Singh Journal of Agricultural and Food Chemistry, 2026 This study reports the biochemical characterization of a novel variant of glutamate decarboxylase (mtGAD), identified from an ethnic food, Kinema. The mtgad gene was cloned from the Kinema metagenome and expressed in a heterologous host, Escherichia coli. The enzyme mtGAD was extracted and purified for biochemical characterization. The enzyme showed optimal activity for γ-aminobutyric acid (GABA) synthesis at 50 °C and pH 4.5. The kinetic parameters of mtGAD were computed to be 29.2 mM Km, 561.8 s–1 kcat, and 23.2 mM–1 s–1 kcat/Km. The in silico protein structure analysis, followed by molecular docking and molecular dynamics simulation, revealed that the binding with pyridoxal-5′-phosphate (PLP; a cofactor for mtGAD) reduces the fluctuations in the structure at high temperatures, contributing to the thermal stability of mtGAD. Furthermore, the conversion of about 60% monosodium glutamate (MSG) into GABA was achieved in approximately 3 h of catalytic reaction with 200 mM MSG, treated with 6.67 U mtGAD. A high turnover number with moderate thermal and pH stability makes mtGAD a potential biocatalyst for GABA production.
Plants as Bioreactors: An Overview Madhu, Alok Sharma, Amandeep Kaur, Deepika Antil, Sudhir P. Singh, Santosh Kumar Upadhyay Plants as Bioreactors an Overview, 2023
Microbial production and transformation of polyphenols Puja Sarkar, Md Minhajul Abedin, Sudhir P. Singh, Ashok Pandey, Amit Kumar Rai Current Developments in Biotechnology and Bioengineering Technologies for Production of Nutraceuticals and Functional Food Products, 2021
Preface Amit Kumar Rai, Sudhir P. Singh, Ashok Pandey, Christian Larroche, Carlos Ricardo Soccol Current Developments in Biotechnology and Bioengineering Technologies for Production of Nutraceuticals and Functional Food Products, 2021
Preface Sudhir P. Singh, Ashok Pandey, Reeta Rani Singhania, Christian Larroche, Zhi Li Biomass Biofuels Biochemicals Advances in Enzyme Catalysis and Technologies, 2020
Basics and roots of synthetic biology Sudhir P. Singh, Shilpi Bansal, Ashok Pandey Current Developments in Biotechnology and Bioengineering Synthetic Biology Cell Engineering and Bioprocessing Technologies, 2018
Preface Sudhir P. Singh, Ashok Pandey, Guocheng Du, Sudesh Kumar Current Developments in Biotechnology and Bioengineering Synthetic Biology Cell Engineering and Bioprocessing Technologies, 2018
Biotransformation of banana pseudostem extract into a functional juice containing value added biomolecules of potential health benefits Indian Journal of Experimental Biology, 2017
Exploring hot-spring metagenomes for the repertoire of carbohydrate-active enzymes and potential bacterial strains for enzyme production R Kumari, N Sharma, KB Singh, SP Singh, AK Rai Heliyon 12 (8), e44925 , 2026 2026
Novel Glutamate Decarboxylase from Ethnic Food Metagenome: A Potential Biocatalyst to Produce a Nonproteinaceous Amino Acid, γ-Aminobutyric Acid SB Sathaye, N Sharma, J Bhaumik, D Sahoo, AK Rai, SP Singh Journal of Agricultural and Food Chemistry 74 (1), 1175-1186 , 2026 2026
Small buds to blooming flowers: organic–inorganic hybrid nanoflowers as immobilization platform and robust system for bio-catalytic production of D-allose S Sharm, SB Sathaye, YN Reddy, J Bhaumik, SP Singh RSC Advances 16, 723-732 , 2026 2026
Overview of bioactive peptides extracted from non-bovine milk and its products R Dolai, P Sarkar, S Padhi, SP Singh, AK Rai Non-Bovine Milk Derived Bioactive Peptides, 1-19 , 2026 2026
Innovative Technologies for Minimization of Plastic Wastes RK Rathour, RK Bhatia, AK Rai, JS Jadaun, SP Singh Biotechnological Approaches for Sustainable Environment Management, 184-209 , 2025 2025
Biosensors in Environmental Monitoring JS Jadaun, PK Yadav, S Shukla, SP Singh, G Kumar Biotechnological Approaches for Sustainable Environment Management, 277-302 , 2025 2025
Microbial Approaches for Lactose Transformation into High‐value Rare Sugars AK Singh, AK Rai, SP Singh Microbial Nutraceuticals: Products and Processes, 167-182 , 2025 2025
Microbial Nutraceuticals: An Overview AK Singh, SK Upadhyay, SP Singh Microbial Nutraceuticals: Products and Processes, 1-21 , 2025 2025 Citations: 1
Biotechnological Approaches for Sustainable Environment Management SP Singh, JS Jadaun, AK Rai Taylor & Francis CRC Press 1, 1-424 , 2025 2025
Microbial Nutraceuticals: Products and Processes SP Singh, SK Upadhyay John Wiley & Sons 1, 1-196 , 2025 2025
Process optimization and partial purification of a highly catalytic xylanase from high-altitude novel Bacillus altitudinis strain for efficient production of functional … LC Phukon, R Chourasia, MM Abedin, A Priya, S Sharma, SP Singh, ... International Journal of Biological Macromolecules , 2025 2025 Citations: 2
Curdlan: A microbial biopolymer of multifarious applications for sustainable environment. Protoplasma S Shukla, K Vishwakarma, AK Singh, PK Yadav, SP Singh, JS Jadaun Protoplasma, 1-13 , 2025 2025
Isolation of a novel Stenotrophomonas strain and identification of methyltransferase genes conferring the high arsenic volatilizing ability D Singh, N Sharma, S Agarwal, SA Khan, V Jain, S Singh, S Roy, ... Applied and Environmental Microbiology, 1-23 , 2025 2025 Citations: 2
A novel cellobiose 2-epimerase from thermal aquatic metagenome for the production of epilactose AK Singh, SB Sathaye, AK Rai, SP Singh Journal of Agricultural and Food Chemistry 73 (16), 9690–9700 , 2025 2025 Citations: 3
A novel xylose isomerase suitable for D-fructose production and synergistic catalysis with D-allulose 3-epimerase for the biosynthesis of D-allulose SN Patel, S Sharma, N Gossai, D Patel, SP Singh Process Biochemistry 151, 52-64 , 2025 2025 Citations: 1
Genetic and functional characterization of a Limosilactobacillus fermentum strain with β-galactosidase activity, isolated from Chhurpi sample of Sikkim AK Singh, T RaheJa, P Sarkar, SB Sathaye, AK Rai, SP Singh Food and Bioproducts Processing 150, 107-117 , 2025 2025 Citations: 1
Unearthing novel and multifunctional peptides in peptidome of fermented chhurpi cheese of Indian Himalayan region R Chourasia, MM Abedin, LC Phukon, P Sarkar, S Sharma, D Sahoo, ... Food Reseach International 201, 115651 , 2025 2025 Citations: 6
Spatiotemporal regulation of anther's tapetum degeneration paved the way for a reversible male sterility system in cotton RK Verma, SP Singh, SP Singh, S Narayan, PC Verma, SV Sawant Plant Biotechnology Journal 23 (2), 532-548 , 2025 2025 Citations: 2
Plants Based Nutraceuticals: An Overview D Bisht, V Bhatt, RKK Arya, S Yadav, D Kumar, SP Singh, SK Upadhyay Herbal Nutraceuticals: Products and Processes, 1-21 , 2024 2024 Citations: 6
Mixed culture fermentation using Lactobacillus delbrueckii and novel Lactiplantibacillus plantarum strains isolated from traditional chhurpi from Sikkim Himalayan region for … P Sarkar, R Chourasia, MM Abedin, AK Singh, S Padhi, SP Singh, AK Rai Biocatalysis and Agricultural Biotechnology 60, 103338 , 2024 2024 Citations: 4
MOST CITED SCHOLAR PUBLICATIONS
Biodegradation of plastics for sustainable environment JS Jadaun, S Bansal, A Sonthalia, AK Rai, SP Singh Bioresource Technology 347, 126697 , 2022 2022 Citations: 288
Prebiotic Oligosaccharides: Special Focus on Fructooligosaccharides, Its Biosynthesis and Bioactivity SP Singh, JS Jadaun, LK Narnoliya, A Pandey Applied Biochemistry and Biotechnology , 2017 2017 Citations: 209
Lactic acid bacteria in the functional food industry: biotechnological properties and potential applications MM Abedin, R Chourasia, LC Phukon, P Sarkar, RC Ray, SP Singh, ... Critical Reviews in Food Science and Nutrition, Taylor & Francis , 2023 2023 Citations: 191
Production, characterization and molecular docking of antioxidant peptides from peptidome of kinema fermented with proteolytic Bacillus spp. S Samurailatpam, S Padhi, P Sarkar, SP Singh, D Sahoo, AK Rai Food Research International 141, 110161 , 2021 2021 Citations: 110
Differential expression of structural genes for the late phase of phytic acid biosynthesis in developing seeds of wheat (Triticum aestivum L.) KK Bhati, S Aggarwal, S Sharma, S Mantri, SP Singh, S Bhalla, J Kaur, ... Plant Science 224, 74-85 , 2014 2014 Citations: 110
Association of satellites with a mastrevirus in natural infection: complexity of wheat dwarf India virus disease J Kumar, J Kumar, SP Singh, R Tuli Journal of virology 88 (12), 7093-7104 , 2014 2014 Citations: 103
Bioprospecting of plant biodiversity for industrial molecules SK Upadhyay, SP Singh John Wiley & Sons , 2021 2021 Citations: 99
Male sterility systems in wheat and opportunities for hybrid wheat development SP Singh, R Srivastava, J Kumar Acta Physiologiae Plantarum 37 (1), 1713 , 2015 2015 Citations: 97
Pattern of iron distribution in maternal and filial tissues in wheat grains with contrasting levels of iron SP Singh, K Vogel-Mikuš, I Arčon, P Vavpetič, L Jeromel, P Pelicon, ... Journal of experimental botany 64 (11), 3249-3260 , 2013 2013 Citations: 87
A novel D-Allulose 3-epimerase gene from the metagenome of a thermal aquatic habitat and D-Allulose production by Bacillus subtilis whole-cell catalysis SN Patel, G Kaushal, SP Singh Applied and Environmental Microbiology 86 (5), e02605-19 , 2020 2020 Citations: 85
Biotechnological approaches for the production of designer cheese with improved functionality C Chourasia, MM Abedin, LC Phukon, D Sahoo, SP Singh, AK Rai Comprehensive Reviews in Food Science and Food Safety, 1-20 , 2020 2020 Citations: 83
Characterization of ACE inhibitory and antioxidant peptides in yak and cow milk hard chhurpi cheese of the Sikkim Himalayan region MM Abedin, R Chourasia, LC Phukon, SP Singh, AK Rai Food Chemistry: X 13, 1-10 , 2022 2022 Citations: 81
Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya N Sharma, J Kumar, MM Abedin, D Sahoo, A Pandey, AK Rai, SP Singh BMC Microbiology 20, 1-17 , 2020 2020 Citations: 79
Metagenomic insights into the taxonomic and functional features of Kinema, a traditional fermented soybean product of Sikkim Himalaya J Kumar, N Sharma, G Kaushal, S Samurailatpam, D Sahoo, AK Rai, ... Frontiers in Microbiology 10, 1744 , 2019 2019 Citations: 78
Improved operational stability of d-psicose 3-epimerase by a novel protein engineering strategy, and d-psicose production from fruit and vegetable residues SN Patel, M Sharma, K Lata, U Singh, V Kumar, RS Sangwan, SP Singh Bioresource Technology 216, 121-127 , 2016 2016 Citations: 74
A novel approach of integrated bioprocessing of cane molasses for production of prebiotic and functional bioproducts M Sharma, SN Patel, K Lata, U Singh, M Krishania, RS Sangwan, ... Bioresource Technology 219, 311–318 , 2016 2016 Citations: 71
Bioactive peptides in fermented foods and their application: a critical review R Chourasia, LC Phukon, MM Abedin, S Padhi, SP Singh, AK Rai Systems Microbiology and Biomanufacturing, 1-22 , 2022 2022 Citations: 66
Plants as Bioreactors for Industrial Molecules SK Upadhyay, SP Singh John Wiley & Sons , 2023 2023 Citations: 64
The phytochemical composition, biological effects and biotechnological approaches to the production of high-value essential oil from geranium LK Narnoliya, JS Jadaun, SP Singh Essential Oil Research: Trends in Biosynthesis, Analytics, Industrial … , 2019 2019 Citations: 63
Whey valorization by microbial and enzymatic bioprocesses for the production of nutraceuticals and value-added products R Chourasia, LC Phukon, MM Abedin, S Padhi, SP Singh, AK Rai Bioresource Technology Reports 19, 101144 , 2022 2022 Citations: 62
