Priyoneel Basu

Scopus Publications

Developing Multilingual Glossaries for STEM Terminology Using AI-NLP
Priyoneel Basu and Sushree Sangita Mohanty
Springer Nature Singapore

Impact of a Single Episode of Different Intensity of White Light Pulse at Night on Masking Response in the Diurnal Mammal, Funambulus pennantii
Vivek Verma, Priyoneel Basu, and Muniyandi Singaravel
Chronobiology in Medicine
Objective: Light affects mammalian circadian rhythms through entrainment and masking. Photic masking increases locomotor activity in diurnal species (positive masking) while reducing activity in nocturnal species (negative masking). Fewer studies have investigated masking in diurnal rodents. We investigated the masking response in a diurnal rodent, <i>Funambulus pennantii</i> when subjected to white light pulses of different intensities at night between zeitgeber time (ZT) 12 and ZT 15.Methods: Adult male squirrels, <i>F. pennantii</i> (n=10/group, three groups) were placed in individual cages with running wheels under standard 12:12 h light-dark (LD) conditions. Recording of the locomotor activity rhythm was carried out using a ClockLab setup. Following a week of stable entrainment to the LD cycle, the animals of each group were given a sham exposure. After 10 days, animals of group I, II, and III were exposed to 3-h white light pulses of 100 lux, 10 lux, and 1 lux, respectively, between ZT 12 and ZT 15. Following the exposure, the animals were allowed to run undisturbed for 7 days.Results: Compared to the shamexposed group, exposure to 3-h 1 lux, 10 lux, and 100 lux artificial light pulses at night caused positive masking, stimulating wheel running in an intensity-dependent manner. Additionally, nighttime light pulses of 100 lux and 10 lux reduced onset accuracy, reduced the amplitude of the rhythm, and also altered the phase angle relationship to light.Conclusion: The positive masking caused by light exposure at night in diurnal squirrels is intensity-dependent up to at least 100 lux and is associated with a dampening of circadian rhythms.

Withanolide-A Accelerates Re-entrainment of Circadian Locomotor Activity Rhythm and Preserves Spatial Memory in Mice Following Acute Phase Inversion of Light-Dark Cycles
Trisha Tiwari, Priyoneel Basu, and Muniyandi Singaravel
Chronobiology in Medicine
Objective: Circadian rhythms are present almost in all living organisms and synchronized with the zeitgebers (time givers), especially lightdark (LD) cycles. The jetlag and rotating shift work schedules lead to desynchronization of the circadian clock with zeitgebers which may cause various health problems. Studies have shown that withanolide-A (WA) is a potent nootropic and neuroprotective agent found in Withania somnifera. Thus, the objective was to study the effect of WA on the circadian locomotor activity rhythm following the phase inversion of 12:12 h LD cycle in mice and its impact on the spatial memory in mice.Methods: Adult male mice were housed in the chronocubicle and recorded circadian locomotor activity rhythm by using ClockLab setup. The experimental mice were administered with WA (10 μmol/kg/day) for 13 consecutive days at about ZT15 and then evoked an acute phase inversion of 12:12 h light-dark cycle. Further, the Barnes maze test was performed to check the effect of WA on spatial memory following the phase inversion.Results: The results showed that WA-treated group took significantly fewer transient cycles (4.66+0.66) for re-entrainment than the control (9.5+0.43) and vehicle-treated (8.33+1.28) mice following the phase inversion. Similarly, WA-treated mice traveled less distance to reach the target zone in the Barnes maze compared to vehicle and control groups.Conclusion: These results suggest that the WA may act as chronobiotic and neuroprotective agent since it decreased the transient cycles for re-entrainment and retaining spatial memory following the phase inversion.

Sirtuins and the circadian clock interplay in cardioprotection: focus on sirtuin 1
Sanjeev Kumar Soni, Priyoneel Basu, Muniyandi Singaravel, Ramaswamy Sharma, Seithikurippu R. Pandi-Perumal, Daniel. P. Cardinali, and Russel J. Reiter
Springer Science and Business Media LLC

Differences in post-chronic jet lag parameters in male and female mice
Trisha Tiwari, Priyoneel Basu, and Muniyandi Singaravel
Informa UK Limited
ABSTRACT Chronic jet lag has deleterious effects on many physiological parameters. Most simulations of chronic jet lag consist of repeated advances only, while real-life conditions may differ. We compared number of transients, activity (α) and rest (ρ), and robustness of rhythm by autocorrelation in male and female AKR mice; before, during, and after simulated chronic jet lag (CJL); and phase angle of entrainment and accuracy before and after CJL, using alternate 9-h advances and delays of the light-dark schedule. Females exhibited no scalloping. Compared to pre-CJL values, α was significantly more in females post-CJL, indicating α decompression. The levels of activity increased during and after CJL, particularly in females, indicating behavioural feedback on the circadian rhythm. Robustness of rhythm (autocorrelation coefficient, and amplitude) was significantly suppressed during CJL in both males and females. Females took more transients than males to re-entrain to the final LD shift at the end of CJL. More transients were needed to re-entrain at the end of CJL, compared to a single shift. The study suggests that animals undergoing CJL incorporating alternating advances and delays are able to avoid α compression associated with jet lag, and also that females are detrimentally affected more than males. Abbreviations: SCN: Suprachiasmatic Nuclei; CJL: Chronic Jet Lag; LD: Light-dark

Risperidone resets the circadian clock in mice
Rajeev Cherukalady, Dhanananajay Kumar, Priyoneel Basu, and Muniyandi Singaravel
Informa UK Limited
Abstract Risperidone is an atypical antipsychotic that is active at multiple dopamine and serotonin receptor subtypes. Based on its high affinity for serotonin receptors, we predicted that it might reset circadian rhythms in a nocturnal rodent. We report temporally differentiated and differential effects of various doses of risperidone on the voluntary locomotor activity rhythm in the Indian field mice, Mus booduga. Risperidone (0.5 mg/kg) elicited phase delays at phases between CT (circadian time) 12 to CT18 and CT0 to CT3, and phase advances at CT6, CT9 and CT21. However, mice injected at CT6 showed maximum advances (1.299 ± 0.286 h), whereas at CT15 showed maximum delays (−1.514 ± 0.312 h). Increasing the dose beyond 0.5 mg/kg at maximally responsive CTs (CT6 and CT15) resulted in progressively smaller but significant shifts. Thus, 0.5 mg/kg is the optimal dose in this species. The fact that risperidone resets the circadian rhythm in a mammal can be extended to clinical studies and used for optimal adjustment of the circadian rhythm in mental disorders. Conversely, risperidone administration for various treatments must be carefully timed to prevent unwanted phase shifts in patients.

The cholinergic forebrain arousal system acts directly on the circadian pacemaker
Glenn R. Yamakawa, Priyoneel Basu, Filomeno Cortese, Johanna MacDonnell, Danica Whalley, Victoria M. Smith, and Michael C. Antle
Proceedings of the National Academy of Sciences
Significance Sleep and wake states are regulated by a variety of mechanisms. One such important system is the circadian clock, which provides temporal structure to sleep and wake. Conversely, changes in behavioral state can influence the phase of the circadian clock. Here we demonstrate that the level of wakefulness is critical for arousal to reset circadian clock phase. We then show that treatments that produce arousal and reset the circadian clock activate the basal forebrain. Finally, we demonstrate that cholinergic input from the basal forebrain is both necessary and sufficient for eliciting this arousal-induced resetting of the circadian clock. These results establish a functional link between the major forebrain arousal center and the circadian system.

Phase shifts to light are altered by antagonists to neuropeptide receptors
Ryan K. Chan, Roxanne Sterniczuk, Yaruuna Enkhbold, Ryan T. Jeffers, Priyoneel Basu, Bryan Duong, Sue-Len Chow, Victoria M. Smith, and Michael C. Antle
Elsevier BV

Variability of behavioral chronotypes of 16 mammalian species under controlled conditions
Roberto Refinetti, Thomas Wassmer, Priyoneel Basu, Rajeev Cherukalady, Varun Kumar Pandey, Muniyandi Singaravel, Claudia Giannetto, and Giuseppe Piccione
Elsevier BV

Activation of M1/4 receptors phase advances the hamster circadian clock during the day
Priyoneel Basu, Adrienne L. Wensel, Reid McKibbon, Nicole Lefebvre, and Michael C. Antle
Elsevier BV

Triptans attenuate circadian responses to light
Priyoneel Basu, Naomi Ie, Adrienne L. Wensel, Joelle D. Baskerville, Victoria M. Smith, and Michael C. Antle
Wiley
Daily exposure to light synchronizes the circadian clock, located in the suprachiasmatic nucleus (SCN), to external day/night cycles. These responses to light can be modified by serotonergic drugs, such as serotonin 5HT1B receptor agonists. Triptans are specific 5HT1B agonists prescribed to treat migraines. Here, we examined the effects of two triptans (zolmitriptan and sumatriptan) on photic phase resetting in Syrian hamsters. Pre‐treatment with intra‐SCN sumatriptan significantly attenuates, and at higher doses completely blocks, phase advances to light during the late night. Pre‐treatment with systemic zolmitriptan significantly attenuates both light‐induced phase advances and phase delays. Neither of these drugs, nor their vehicles, causes phase shifts on their own. Pre‐treatment with zolmitriptan also significantly reduces the expression of light‐induced c‐fos in the SCN. Neither zolmitriptan nor vehicle alone induces significant c‐fos expression in the SCN. Finally, pre‐treatment with zolmitriptan does not attenuate phase shifts to intra‐SCN N‐methyl‐d‐aspartate injections, indicating that the mechanism of action for zolmitriptan is likely to be through activation of presynaptic 5HT1B receptors on retinal terminals, thereby decreasing light‐induced neurotransmitter release. As triptans are commercially available medications, there is potential for their use in blocking unwanted photic phase shifting during shift‐work or jet‐lag. Additionally, triptans may also affect the circadian clock in patients receiving them regularly for migraines. Finally, our results may hint at the mechanism by which triptans can alleviate the photophobia that frequently accompanies migraines, namely by activating 5HT1B receptors on retinal terminals elsewhere in the brain, and thereby diminishing visually‐evoked neurotransmitter signalling in those areas.

Experimental quantification of improvement during circadian wheel running in the Indian field mouse, Mus terricolor: Theoretical uses
Priyoneel Basu, Muniyandi Singaravel, and Roberto Refinetti
Informa UK Limited
We quantified motor learning during voluntary wheel-running activity in the Indian pygmy field mouse, Mus terricolor. Wheel running in naive adults was monitored using the Clocklab system. A group of mice having 15 days prior wheel-running experience served as the control. The daily maximum wheel activity for the naive group increased from 21 ± 7 counts/min to 62 ± 4 counts/min in 15 days. The experienced group exhibited 62 ± 1 counts/min throughout the experiment. A significant correlation between days of wheel running and natural log of the highest count/min existed in the experimental group, but not in the experienced group. Thus, improvement in wheel running follows a logarithmic learning curve. Several research applications for this quantification have been discussed. The most important outcome of this quantification is that it primes the mice for a study in which the retention period for this motor learning, i.e. the time taken to “forget” motor learning during wheel running will be elucidated.

Effects of lighting condition on circadian behavior in 5-HT<inf>1A</inf> receptor knockout mice
Victoria M. Smith, Ryan T. Jeffers, Brendan B. McAllister, Priyoneel Basu, Richard H. Dyck, and Michael C. Antle
Elsevier BV

Fisetin, a dietary flavonoid, attenuates hyperammonemia and improves circadian locomotor deficits, redox balance, and astrocytic markers in rats
Perumal Subramanian, Murugesan Jayakumar, Muniyandi Singaravel, Dhanananajay Kumar, Priyoneel Basu, Jaime Jacqueline Jayapalan, and Onn Haji Hashim
Elsevier BV

Reproductive seasonality in the Indian pygmy field mouse, Mus terricolor
Sweta Arora, Priyoneel Basu, Poonam Singh, and Chandana Haldar
Informa UK Limited
The Indian pygmy field mouse, Mus terricolor, is a tiny, yet economically and ecologically important crop pest found throughout South-East Asia. There are no systematic reports exploring its reproductive physiology. We report the presence of distinct periods of annual reproductive activity and quiescence in M. terricolor. Body weight in males and females, relative weights of testis, epididymis and seminal vesicle in males, ovarian and uterine weight in females, gonadal histomorphic changes, testicular and ovarian cholesterol, sialic acid in epididymis, fructose in seminal vesicle, uterine protein content, melatonin in males and females, testosterone in males, estradiol, and progesterone in females were studied over a period of three years in both wild-caught and lab-acclimated mice. The number of Graafian follicles and corpora lutea, and plasma estradiol and progesterone, along with relative weights of ovary and uterus in females exhibited a peak in the months of October–January, compared to June. Based on histomorphic and hormonal status, the major reproductively active season is the winter (short-day breeding). There is a brief period of sub-maximal reproductive activity in April. M. terricolor is reproductively inactive in the summer, monsoons, and autumn. The results establish M. terricolor as a seasonal breeder in the field, with interesting implications for pest management.

Variations in the rate and direction of re-entrainment to acute simulated jet lag in the diurnal North Indian palm squirrel
Dhanananajay Kumar, Priyoneel Basu, and Muniyandi Singaravel
Informa UK Limited
We report the presence of variations in the rate and direction of re-entrainment to acute simulated jet lag in wild-caught North Indian palm squirrels (Funambulus pennanti). Adult laboratory and wheel-acclimated (Lafayette, USA) males (N = 30) were entrained to a 12:12 h light/dark (LD) schedule, followed by a 9 h advance of the LD schedule to simulate acute jet lag. The direction and rate of re-entrainment, i.e. the number of orthodromic (advancing) and antidromic (delaying) transients, the acrophases of daily rhythms in each case, duration of activity (α) and phase angle relationship (ψ) were noted using Clocklab (Coulbourn, USA). The squirrels exhibited distinct variations in their response to simulated acute jet lag, re-entraining either slowly (14/30) or rapidly (10/30) by advancing transients, or undergoing antidromic re-entrainment slowly (5/30), and, in only one individual, rapidly. Acrophases re-entrained slower than onsets. α underwent compression during re-entrainment, particularly during fast orthodromic responses. ψ was not significantly different in any of the groups before or after re-entrainment. This phenomenon has scope for further investigation and highlights the need for individual assessment of re-entrainment patterns before taking preventive measures for jet lag.

Slow and fast orthodromic and antidromic variants in acute 9-h jet-lagged pygmy field mice

Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra hircus during summer and winter

Acceleration of re-entrainment during a 6-h acute jet lag simulation by 5-hydroxy-l-tryptophan in pygmy field mice
Priyoneel Basu and Muniyandi Singaravel
Informa UK Limited
L-5-Hydroxytryptophan (5-HTP), the precursor of serotonin, is known to cause temporally differentiated phase shifts. However, its effects on acceleration of re-entrainment have not been demonstrated before. We report significant acceleration of re-entrainment to a 6-h advanced light dark schedule by 50 mg/kg 5-HTP (∼8 transients reduced to ∼3 transients) injected at ∼ZT 6 in the nocturnal Indian pygmy field mouse, Mus terricolor. The mechanistic explanation is currently unknown, but this probably occurs through serotonergic phase and period modifications. 5-HTP is thus potentially useful as a chronobiotic in circadian rhythm disorders.

5-Hydroxy-L-tryptophan entrains the free-running rhythm in constant darkness in pygmy mice
Priyoneel Basu and Muniyandi Singaravel
Informa UK Limited
L-5-Hydroxytryptophan (5-HTP), the precursor of serotonin, has phase-resetting ability under constant conditions in mammals. However, it is not known whether it can entrain a free-running rhythm. We report that 5-HTP (50 mg/kg) entrains the free-running rhythm of Mus terricolor to 24 h, when presented once in 24 h at zeitgeber time (ZT) 6, corresponding to its previously known circadian time (CT) of maximum advance in complete darkness (CT 6), on the last pre-intervention day. This entrainment is accompanied by shortening of the period and visible after withdrawal of injections. After-effects may persist for a few days. The mechanistic explanation is currently unknown, but this probably occurs through serotonergic phase and period modifications. 5-HTP is thus potentially useful as a chronobiotic in circadian rhythm disorders.

Potentiation of light-induced phase shifts by 5-hydroxy-L-tryptophan in Pygmy field mice: A preliminary report
Priyoneel Basu and Muniyandi Singaravel
Informa UK Limited
Serotonergic agents usually attenuate light-induced phase shifts in nocturnal species. L-5-Hydroytrytptophan (5-HTP) is a non-specific serotonergic agonist and the natural precursor of serotonin. We investigated the ability of 5-HTP to potentiate or attenuate light-induced phase shifts using a comparatively lower intensity of light (∼70 lux). Mice free-running in DD were given either light exposure at circadian times (CTs) 15 and 21 with saline (light-exposed control) or 50 mg/kg of 5-HTP (Himedia) at 0.5 circadian h prior to light exposure. L-5-Hydroytrytptophan potentiated the phase advance of the rhythm caused by light at CT 21; however, 5-HTP fails to significantly attenuate/potentiate phase delays. Total activity increased during phase advance potentiation, compared to control, indicating that this phenomenon may possibly be at least partially behavioral arousal-dependent. These preliminary findings are interesting and unexpected; further experimentation is necessary, and the results indicate possible scope for use of 5-HTP in chronotherapy.

Accurate and precise circadian locomotor activity rhythms in male and female Indian pygmy field mice, Mus terricolor
Priyoneel Basu and Muniyandi Singaravel
Informa UK Limited
We report some basic circadian characteristics in Mus terricolor, the nocturnal pygmy field mouse. Both male and female M. terricolor entrain well to a 12:12 light:dark schedule. Activity is completely nocturnal in distribution in LD. ρ and α in LD differ significantly between males and females. Free-running circadian locomotor activity rhythm in DD was 23.69 h (males) and 23.46 h (females). In LD, ψ was −0.20 h and −0.15 h in males and females, respectively. Rhythms were accurate (6.02 in males/7.30 in females [h−1]) and precise (4.83 in males /4.74 in females [h−1]). Fifteen-minute perturbations with ∼70 lux white light in DD led to predictable phase advances and delays. Mus terricolor took ∼7 days to re-entrain to a 6 h advance in the ambient light schedule, with rapid resynchronization to a 6 h phase delay. These results indicate that M. terricolor is a good model animal for in-depth research in circadian biology.

Effect of chronic jet lag after induction of Dalton's lymphoma in male and female mice
Priyoneel Basu, Ratnakar Tripathi, Rajnikant Mishra, and Muniyandi Singaravel
Informa UK Limited
Biological rhythms are ubiquitous phenomena that enable an organism to temporally adapt to the environment. Jet lag occurs during rapid transmeridial transport and disrupts the normal biological rhythm. Disruption of the biological rhythm is known to reduce survival and hasten tumorigenesis in male mice. Dalton's lymphoma (DL) is a spontaneous and highly invasive T-cell lymphoma that develops as an ascitic tumor in murines. DL was induced by serial implantation of live ascite cells in laboratory-acclimated age-matched male and female AKR mice. The mice were then subjected to simulated chronic jet lag (CJL) by rapidly and alternately advancing and delaying the ambient light dark cycle by 8 h every 2 days. Females, but not males, with DL became arrhythmic after exposure to simulated CJL. Survival was significantly curtailed in both male and female mice with DL + CJL compared to survival in mice with DL alone or CJL alone (∼16 days after DL induction in mice and facing jet lag, and ∼28 days after DL induction in mice bearing but not undergoing jet lag simulation). These results suggest that female mice may be more at risk from jet lag associated effects if they have cancer prior to facing jet lag, and DL + CJL may detrimentally impact the female circadian clock more than that of the males.

Effect of induced Dalton's lymphoma on circadian locomotor activity rhythm of adult male mice
Brij Bharti, Priyoneel Basu, Rajnikant Mishra, and Muniyandi Singaravel
Informa UK Limited
Dalton's lymphoma (DL) is a spontaneous and highly invasive T-cell lymphoma that develops as an ascitic tumor in murines. DL was induced by serial implantation of live ascite cells in laboratory-acclimated age-matched male AKR mice. The effects of DL on the locomotor activity rhythm were studied under 12:12 h light:dark (LD) cycle. A significant decrease in the total and peak wheel-running activity was seen after the induction of DL, in comparison with the vehicle-injected control group. Durations of activity and rest were altered in DL mice, with a sharp daily increase in the duration of rest in the DL group. Circadian time period and acrophase of the rhythm remained similar in both control and DL mice. The mean estimate of statistic of rhythmicity (MESOR) and the amplitude of the locomotor activity rhythm were significantly lesser in post-DL-induced mice. A reduction in the span of the M1 and M2 motor cortex regions in DL mice and anatomical changes in the dentate gyrus were also seen. Thus, the effects of DL on the circadian clock seem restricted and mouse can still synchronize to an LD cycle. Impact of DL extends to motor cortices and may be the cause of the dampening of the amplitude of the rest-activity rhythm.

L-5-hydroxytryptophan resets the circadian locomotor activity rhythm of the nocturnal indian pygmy field mouse, Mus terricolor
Priyoneel Basu, Muniyandi Singaravel, and Chandana Haldar
Springer Science and Business Media LLC

Priyoneel Basu

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