Exploring the Discrepancies in the Biological Activities of Extracts From Guadua angustifolia Var. Bicolor Londoño Collected in Two Different Sites João Kazlauckas, Maria Tereza Grombone Guaratini, Paulo Roberto H. Moreno Chemistry and Biodiversity, 2025 Guadua angustifolia var. bicolor Lodoño is a Colombian bamboo species that has shown promising medicinal properties. This study aims to investigate the differences in the chemical composition and biological activity of extracts from two different populations of G. angustifolia var. bicolor, collected in Bauru and Tatuí, Brazil. The extracts were analyzed using high‐performance liquid chromatography‐tandem mass spectrometry, and their anti‐tyrosinase and antioxidant activities were evaluated using the enzyme tyrosinase and diphenyl‐1‐picrylhydrazyl, respectively. The crude extract was prepared using a Soxhlet apparatus, followed by a solid‐liquid extraction and, finally, a column chromatography. The results showed that the subfractions from the two populations had different chemical compositions, which may explain their different biological activities. The Bauru extracts contained higher levels of phenolic compounds and fatty acids, which are known for their antioxidant and anti‐tyrosinase activities. These extracts were also found to be the most active samples. These findings suggest that the chemical composition of bamboo extracts can vary depending on the origin of the sample.
Essential oil vapor phase approach for antimicrobial evaluation using Hesperozygis myrtoides (A. St.-Hill. ex Benth.) Epling (Lamiaceae) as a model MARCOS AURÉLIO A. PEREIRA, ADILSON SARTORATTO, CRISTINA MAIRA T.S. MIGUEL, CYNTHIA MURAKAMI, FABIANA L. SILVA, et al. Anais Da Academia Brasileira De Ciencias, 2024 The demand for less hazardous and environmentally friendly surface disinfectants is growing and essential oils may be efficient alternatives. Vapor phase assay is a promising technique which explore the volatility of essential oils for determining the antimicrobial activity. In this study, we modified the conditions of the inverted Petri dish technique to evaluate the minimum inhibitory concentrations (MIC). As a proof of concept, the antimicrobial activity of Hesperozygis myrtoides essential oil (HM) was studied for the first time in the vapor phase. According to the established protocol the oil volatilization dynamics was used to determine the major compounds in the vapor phase. After 270 min, the relative percentage was like that observed in the crude oil analysis. The HM was also evaluated for safety (IC50=921.2 µg ml-1), being considered safe in case of acute oral exposure. The oil vapors completely inhibited the growth of Staphylococcus aureus (MIC=0.39 mg l-1), Candida albicans (MIC=0.83 mg l-1), Aspergillus brasiliensis (MIC=378 mg l-1) and Bacillus subtilis (MIC=704.8 mg l-1). This protocol offers an economical and efficient alternative for antimicrobial activity and safety determination of essential oils, can be used for the evaluation of several applications of essential oils, as disinfectant agent in inhabited environments.
Embelin potentiates venetoclax-induced apoptosis in acute myeloid leukemia cells Catarina Sofia Mateus Reis-Silva, Paola Cristina Branco, Keli Lima, Fabiana Lima Silva, Paulo Roberto Hrihorowitsch Moreno, et al. Toxicology in Vitro, 2021 Acute myeloid leukemia (AML) belongs to a group of hematological cancer whose relapse cases are often associated with chemoresistance that impairs treatment success and contributes to a poor outcome. For this reason, there is an urgent need for the development of new therapeutic strategies. Herein, we explore the combination of venetoclax, a BCL2 inhibitor, and embelin, an XIAP inhibitor, in the AML cell lines. Combinatory treatment of venetoclax and embelin potentiated cytotoxic effects of these drugs, demonstrating that both in combination present lower IC50 values than single treatment of either venetoclax or embelin alone in both cell lines analyzed. The combinatory treatment further increased the apoptosis-inducing properties of both compounds. Computer simulations suggest that embelin binds to both BIR2 and BIR3 domains of XIAP, reinforcing this inhibitory apoptosis protein as an embelin target. Although all AML cell lines presented similar basal levels of XIAP, the combinatory treatment effectively inhibited XIAP expression in OCI-AML3 cells. In conclusion, the inhibition of both apoptosis inhibitory players, BCL2 and XIAP, by venetoclax and embelin, respectively, potentiated their cytotoxic effects in AML cell lines.
Influence of Menstrual Cycle on Aromatic Composition Behavior After Skin Application by Gas Chromatography/Mass Spectrometry Journal of Cosmetic Science, 2021
Chemical composition and in vitro antiprotozoal activity of the volatile oil from leaves of annona crassiflora mart. (Annonaceae) Pharmacologyonline, 2013
RECENT SCHOLAR PUBLICATIONS
Exploring the Discrepancies in the Biological Activities of Extracts From Guadua angustifolia Var. Bicolor Londoño Collected in Two Different Sites J Kazlauckas, MTG Guaratini, PRH Moreno Chemistry & Biodiversity 22 (9), e202500174 , 2025 2025 Citations: 2
Chemical composition and antimicrobial properties of essential oils from two Guadua species (Poaceae, Bambusoideae) DCS de Oliveira, FA Nunes, T Bílek, C Murakami, ... Advances in Bamboo Science 12, 100171 , 2025 2025 Citations: 1
Exploring Myrsine umbellata : a comprehensive analysis of phytochemicals, antioxidant potential, antityrosinase effect, and cytotoxic activities across distinct plant … FL Silva, PC Branco, A Do Espírito Santo, N de Oliveira Santos, ... Journal of Herbs, Spices & Medicinal Plants 31 (2), 209-223 , 2025 2025 Citations: 1
Chemical composition and antimicrobial properties of essential oils from two Guadua species (Poaceae, Bambusoideae) D Oliveira, FA Nunes, T Bílek, C Murakami, MTG Guaratini, PRH Moreno Advances in Bamboo Science 12, 1-16 art. 100171 , 2025 2025
Identificação dos metabólitos secundários em extratos de guadua angustifolia kunth FB Reis, J Kazlauckas, PRH Moreno Resumos , 2025 2025
Estudo da Composição Química das Folhas de Guadua Angustifolia Var. Bicolor Lodoño (Bambusoideae: Poaceae) Pormeio de CLAE-EM com auxílio do GNPS J Kazlauckas, PRH Moreno Anais 11º Workshop do Programa de Mestrado Profissional Tecnologia em … , 2024 2024 Citations: 1
Essential oil vapor phase approach for antimicrobial evaluation using Hesperozygis myrtoides (A. St.-Hill. ex Benth.) Epling (Lamiaceae) as a model MAA Pereira, A Sartoratto, C MIGUEL, TS MAIRA, C MURAKAMI, ... Anais da Academia Brasileira de Ciências 96, e20231370 , 2024 2024 Citations: 1
Estudo da composição química das folhas de Guadua Angustifolia Var. Bicolor Lodoño (Bambusoideae: Poaceae) por meio de CLAE-EM com auxílio do GNPS J Kazlauckas, PRH Moreno 11º Workshop do Programa de Mestrado Profissional Tecnologia em Química e … , 2023 2023
Índice de retenção linear experimental para extraíveis orgânicos semivoláteis ÂO Francisco, C Blatt, LM Yataco, PRH Moreno 11º Workshop do Programa de Mestrado Profissional Tecnologia em Química e … , 2023 2023
Identificação dos extraíveis semivolátéis presentes nos componentes individuais do material de embalagem de um spray nasal ÂO Francisco, C Blatt, LM Yataco, ALB Shimada, DS Freitas, PRH Moreno Anais 10º Workshop do mestrado profissional: tecnologia em química e … , 2022 2022
Antioxidant and photoprotective properties of neotropical bamboo species MT Grombone-Guaratini, CM Furlan, PS Lopes, KP Barsalobra, ... Plant Antioxidants and Health, 231-265 , 2022 2022 Citations: 4
Dereplication of Phenolics from Cardiospermum corindum by Countercurrent Chromatography Combined with Liquid Chromatography-Electrospray Mass … FL Silva, PRH Moreno, ACHF Sawaya, GC de Freitas Lemeszenki, ... Revista Brasileira de Farmacognosia 32 (2), 280-285 , 2022 2022 Citations: 3
Isolamento e Identificação de Compostos com Atividade Antioxidante e Anti‐Tirosinase em Extratos de Merostachys pluriflora Munro Ex EG Camus (Poaceae: Bambusoideae) MM Sobrinho, PRH Moreno Anais 10◦ Workshop Do Mestrado Profissional: Tecnologia Em Química e … , 2022 2022 Citations: 1
Isolamento e identificação de compostos com atividade antioxidante e anti-tirosinase em extratos de Merostachys Pluriflora Munro Ex EG Camus (Poaceae: Bambusoideae) M Martins Sobrinho, PRH Moreno Workshop Mestrado Profissional, Tecnologia em Química e Bioquímica , 2022 2022
Workshop Mestrado Profissional, Tecnologia em Química e Bioquímica PRH Moreno, MS Baptista, DFS Petri 2022
Atividade Antioxidante e Anti-Tirosinase Nas Folhas de Guadua Angustifolia Var. Bicolor Londoño (Bambusoideae: Poaceae) JKC Corrêa, PRH Moreno Workshop do Mestrado Profissional–Universidade de São Paulo, Instituto de … , 2022 2022 Citations: 1
Óleos essenciais e sua aplicabilidade: o potencial da Mata Atlântica Paulista PRH Moreno Alquimista, 5-8 , 2022 2022
Composição química, atividade antimicrobiana de do óleo essencial de Conobea scoparioides e sua associação com conservantes sintéticos para utilização em formulações cosméticas CMTS Miguel, CH Serra, MT Scotti, PRH Moreno Workshop do Programa de Mestrado Profissional Tecnologia em Química e … , 2021 2021
Avaliação do potencial anti-tirosinase e citotóxico de extratos de Pimenta pseudocaryophyllus (GOMES) landrum RO Mota, PRH Moreno, C Murakami, MCM Young, PC Branco, ... Workshop do Programa de Mestrado Profissional Tecnologia em Química e … , 2021 2021
Embelin potentiates venetoclax-induced apoptosis in acute myeloid leukemia cells CSM Reis-Silva, PC Branco, K Lima, FL Silva, PRH Moreno, V Guallar, ... Toxicology in Vitro 76, 105207 , 2021 2021 Citations: 12
MOST CITED SCHOLAR PUBLICATIONS
Effects of inhaled Linalool in anxiety, social interaction and aggressive behavior in mice VM Linck, AL da Silva, M Figueiró, EB Caramao, PRH Moreno, ... Phytomedicine 17 (8-9), 679-683 , 2010 2010 Citations: 402
Inhaled linalool-induced sedation in mice V de Moura Linck, AL da Silva, M Figueiró, AL Piato, AP Herrmann, ... Phytomedicine 16 (4), 303-307 , 2009 2009 Citations: 327
Cell and tissue cultures of Catharanthus roseus : A literature survey: II. Updating from 1988 to 1993 PRH Moreno, R van der Heijden, R Verpoorte Plant cell, tissue and organ culture 42 (1), 1-25 , 1995 1995 Citations: 312
Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus P Sartorelli, AD Marquioreto, A Amaral‐Baroli, MEL Lima, PRH Moreno Phytotherapy research 21 (3), 231-233 , 2007 2007 Citations: 249
Effect of terpenoid precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus PRH Moreno, R Van der Heijden, R Verpoorte Plant Cell Reports 12 (12), 702-705 , 1993 1993 Citations: 191
Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells R Verpoorte, R van der Heijden, PRH Moreno The alkaloids: chemistry and pharmacology 49, 221-299 , 1997 1997 Citations: 183
Alcalóides: generalidades e aspectos básicos AT Henriques, VA Kerber, PRH Moreno Farmacognosia: da planta ao medicamento , 1999 1999 Citations: 125
Composição química dos frutos de Campomanesia adamantium (Cambessédes) O. Berg MI Vallilo, LCA Lamardo, ML Gaberlotti, E Oliveira, PRH Moreno Food Science and Technology 26 (4), 805-810 , 2006 2006 Citations: 113
Taxonomic significance of alkaloids and iridoid glucosides in the tribe Psychotrieae (Rubiaceae) S Lopes, GL Von Poser, VA Kerber, FM Farias, EL Konrath, P Moreno, ... Biochemical Systematics and Ecology 32 (12), 1187-1195 , 2004 2004 Citations: 106
Ervatamia coronaria: chemical constituents and some pharmacological activities AT Henriques, AA Melo, PRH Moreno, LL Ene, JAP Henriques, ... Journal of ethnopharmacology 50 (1), 19-25 , 1996 1996 Citations: 103
A computer-assisted approach for chemotaxonomic studies—diterpenes in Lamiaceae SAV Alvarenga, JP Gastmans, G do Vale Rodrigues, PRH Moreno, ... Phytochemistry 56 (6), 583-595 , 2001 2001 Citations: 88
Effects of elicitation on different metabolic pathways in Catharanthus roseus (L.) G. Don cell suspension cultures PRH Moreno, C Poulsen, R van der Heijden, R Verpoorte Enzyme and microbial technology 18 (2), 99-107 , 1996 1996 Citations: 85
Purification and cDNA Cloning of Isochorismate Synthase from Elicited Cell Cultures of Catharanthus roseus LJP Van Tegelen, PRH Moreno, AF Croes, R Verpoorte, GJ Wullems Plant physiology 119 (2), 705-712 , 1999 1999 Citations: 81
Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures PRH Moreno, R van der Heijden, R Verpoorte Plant cell reports 14 (2), 188-191 , 1994 1994 Citations: 81
Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity CAT Siqueira, J Oliani, A Sartoratto, CL Queiroga, PRH Moreno, ... Revista Brasileira de Farmacognosia 21 (1), 33-40 , 2010 2010 Citations: 79
In vitro screening for antiplasmodial activity of isoquinoline alkaloids from Brazilian plant species DCH Fischer, NC de Amorim Gualda, D Bachiega, CS Carvalho, FN Lupo, ... Acta tropica 92 (3), 261-266 , 2004 2004 Citations: 74
Influence of temperature on growth and ajmalicine production by Catharantus roseus suspension cultures HJG Ten Hoopen, JL Vinke, PRH Moreno, R Verpoorte, JJ Heijnen Enzyme and Microbial Technology 30 (1), 56-65 , 2002 2002 Citations: 70
In vitro antioxidant and photoprotective activity of five native Brazilian bamboo species KB Wroblewska, AR Baby, MTG Guaratini, PRH Moreno Industrial Crops and Products 130, 208-215 , 2019 2019 Citations: 69
Composição química dos frutos de Campomanesia xanthocarpa Berg-Myrtaceae MI Vallilo, PRH Moreno, E Oliveira, LCA Lamardo, ML Garbelotti Food Science and Technology 28 (suppl), 231-237 , 2008 2008 Citations: 66
Pilocarpus spp.: A survey of its chemical constituents and biological activities AP Santos, PRH Moreno Revista brasileira de ciências farmacêuticas 40 (2), 116-137 , 2004 2004 Citations: 66