Xianghui Qi

Scopus Publications

Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose
Hossain M. Zabed, Suely Akter, Yuehui Tian, Mudasir A. Dar, Junhua Yun, Mei Zhao, Arthur J. Ragauskas, Jia Li, and Xianghui Qi
Elsevier BV



Random mutagenesis and transcriptomics-guided rational engineering in Zygosaccharomyces rouxii for elevating D-arabitol biosynthesis
Guoyan Zhang, Hossain M Zabed, Yufei Zhang, Jia Li, Junhua Yun, and Xianghui Qi
Elsevier BV



Tailoring whole slurry bioprocessing for sugary stovers to augment sugar production by integrating soluble and insoluble carbohydrates
Hossain M. Zabed, Suely Akter, Junhua Yun, Mostafa E. Elshobary, Sr Subroto Haldar, Mei Zhao, Faisal I. Chowdhury, Jia Li, and Xianghui Qi
Elsevier BV



Exploiting the biocatalytic potential of co-expressed L-fucose isomerase and D-tagatose 3-epimerase for the biosynthesis of 6-deoxy-L-sorbose
Muhammad Waheed Iqbal, Xinrui Tang, Tahreem Riaz, Shahid Mahmood, Yufei Zhang, Mei Zhao, Junhua Yun, Jia Li, and Xianghui Qi
Elsevier BV



Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3-Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates
Lei Zhou, Yufei Zhang, Tingting Chen, Junhua Yun, Mei Zhao, Hossain M. Zabed, Cunsheng Zhang, and Xianghui Qi
American Chemical Society (ACS)
Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, namely, 3-hydroxypropionic acid (3-HP). First, the heterologous pathways were introduced into E. coli by coexpressing glycerol-3-P dehydrogenase and glycerol-3-P phosphatase in both single and fusion (gpdp12) forms, making the strain capable of synthesizing glycerol from glucose. Subsequently, a glycerol dehydratase (DhaB123-gdrAB) and an aldehyde dehydrogenase (GabD4) were overexpressed to convert glycerol into 3-HP. A fine-tuning between glycerol synthesis and its conversion into 3-HP was successfully established by 5'-untranslated region engineering of gpdp12 and dhaB123-gdrAB. The strain was further metabolically modulated to successfully prevent glycerol flux outside the cell and into the central metabolism. The finally remodulated chassis produced 32.91 g/L 3-HP from the cellulosic hydrolysate of stover during fed-batch fermentation.


Sustainable bio-manufacturing of D-arabitol through combinatorial engineering of Zygosaccharomyces rouxii, bioprocess optimization and downstream separation
Xiaolan Li, Hossain M. Zabed, Junhua Yun, Yufei Zhang, Mei Zhao, Cunsheng Zhang, Zhen Ouyang, Jia Li, and Xianghui Qi
Elsevier BV



Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters
Mei Zhao, Jie Shang, Jiaojiao Chen, Hossain M. Zabed, and Xianghui Qi
MDPI AG
Glycolate plays an important role as a platform chemical in both polymeric material and cosmetic industries. However, the microbial production of glycolate often encounters challenges associated with unbalanced metabolic flux, leading to a notably low titer. Additionally, the use of expensive inducers, such as IPTG, contributes to an increase in the overall production cost. To address these issues, the key enzymes involved in the glycolate biosynthetic route, including citrate synthase (gltA), isocitrate lyase (aceA), isocitrate dehydrogenase kinase/phosphatase (aceK) and glyoxylate reductase (ycdW), were overexpressed in E. coli under the control of inducible promoters with varying strengths in order to determine the optimal combination. Subsequently, the glycolate pathway was further modulated by replacing inducible promoters with various constitutive synthetic promoters. Through this systematic optimization, the best strain, named Mgly4T1562, produced 3.02 g/L glycolate with 97.32% theoretical yield in shake-flask cultivation. The titer further increased to 15.53 g/L in a fed-batch experiment. Notably, this study marks the first successful utilization of synthetic promoters in tuning the glycolate biosynthetic pathway for glycolate biosynthesis. The strategy presented in this research holds significant promise for facilitating the cost-effective and industrially viable production of glycolate without the need for expensive inducers.


A comprehensive review of recent advances in the characterization of L-rhamnose isomerase for the biocatalytic production of D-allose from D-allulose
Shahid Mahmood, Muhammad Waheed Iqbal, Xinrui Tang, Hossain M. Zabed, Ziwei Chen, Cunsheng Zhang, Yuvaraj Ravikumar, Mei Zhao, and Xianghui Qi
Elsevier BV



Fucoidan-based nanomaterial and its multifunctional role for pharmaceutical and biomedical applications
Muhammad Waheed Iqbal, Tahreem Riaz, Shahid Mahmood, Muhammad Bilal, Muhammad Faisal Manzoor, Sarmad Ahmad Qamar, and Xianghui Qi
Informa UK Limited
Abstract Fucoidans are promising sulfated polysaccharides isolated from marine sources that have piqued the interest of scientists in recent years due to their widespread use as a bioactive substance. Bioactive coatings and films, unsurprisingly, have seized these substances to create novel, culinary, therapeutic, and diagnostic bioactive nanomaterials. The applications of fucoidan and its composite nanomaterials have a wide variety of food as well as pharmacological properties, including anti-oxidative, anti-inflammatory, anti-cancer, anti-thrombic, anti-coagulant, immunoregulatory, and anti-viral properties. Blends of fucoidan with other biopolymers such as chitosan, alginate, curdlan, starch, etc., have shown promising coating and film-forming capabilities. A blending of biopolymers is a recommended approach to improve their anticipated properties. This review focuses on the fundamental knowledge and current development of fucoidan, fucoidan-based composite material for bioactive coatings and films, and their biological properties. In this article, fucoidan-based edible bioactive coatings and films expressed excellent mechanical strength that can prolong the shelf-life of food products and maintain their biodegradability. Additionally, these coatings and films showed numerous applications in the biomedical field and contribute to the economy. We hope this review can deliver the theoretical basis for the development of fucoidan-based bioactive material and films. Graphical Abstract


Efficient biosynthesis of 3-hydroxypropionic acid from glucose through multidimensional engineering of Escherichia coli
Yufei Zhang, Junhua Yun, Guoyan Zhang, Amreesh Parvez, Lei Zhou, Hossain M. Zabed, Jia Li, and Xianghui Qi
Elsevier BV



Rewiring Bacillus subtilis and bioprocess optimization for oxidoreductive reaction-mediated biosynthesis of D-tagatose
Guoyan Zhang, Yingfeng An, Hossain M Zabed, Junhua Yun, Amreesh Parvez, Mei Zhao, Cunsheng Zhang, Yuvaraj Ravikumar, Jia Li, and Xianghui Qi
Elsevier BV



Consolidate Processing for Improvement of Ethanol Production from Food Waste
Zhihui Ling, Shang Wang, Zhongzhan Leng, Chengmei Li, Wenhui Zhang, Xinxin Kang, Xianghui Qi, and Cunsheng Zhang
American Chemical Society (ACS)



Enhanced fermentable sugar production in lignocellulosic biorefinery by exploring a novel corn stover and configuring high-solid pretreatment conditions
Hossain M. Zabed, Suely Akter, Mudasir A. Dar, Jamila A. Tuly, Mukesh Kumar Aswathi, Junhua Yun, Jia Li, and Xianghui Qi
Elsevier BV



Systematic functional analysis and potential application of a serine protease from cold-adapted Planococcus bacterium
Weijun Leng, Xiaoyun Wu, Xianghui Qi, Hongying Liu, Li Yuan, and Ruichang Gao
Tsinghua University Press



Impact of particle size of cell carrier on caproate fermentation in a cell immobilized system: Focusing on the improvement of caproate production in batch and continuous operation modes
Yuxuan Sun, Jiaqi Fu, Yan Liu, Wenhui Zhang, Chengmei Li, Hossain M. Zabed, Mei Zhao, Weimin Liu, Shuhao Huo, Cunsheng Zhang,et al.
Elsevier BV



Towards the sustainable conversion of corn stover into bioenergy and bioproducts through biochemical route: Technical, economic and strategic perspectives
Hossain M. Zabed, Suely Akter, Junhua Yun, Guoyan Zhang, Mei Zhao, M. Mofijur, Mukesh Kumar Awasthi, M.A. Kalam, Arthur Ragauskas, and Xianghui Qi
Elsevier BV



Techno-economic and life cycle assessments for bioenergy recovery from acid-hydrolyzed residues of sugarcane bagasse in the biobased xylose production platform
Shiyou Pan, Hossain M. Zabed, Mei Zhao, Xianghui Qi, and Yutuo Wei
Elsevier BV



A novel maltooligosaccharide-forming α-amylase from Bacillus cereus and its application in the preparation of maltopentaose product
Shiyou Pan, Guiping Wang, Chang Sun, Liqin Du, Xianghui Qi, and Yutuo Wei
Elsevier BV



Rechargeable metal-metal alkaline batteries: Recent advances, current issues and future research strategies
Jahidul Islam, Rubel Anwar, Mahmud Shareef, Hossain M. Zabed, J.N. Sahu, Xianghui Qi, Mayeen Uddin Khandaker, Arthur Ragauskas, Imed Boukhris, Md Rezaur Rahman,et al.
Elsevier BV



High-level co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Metabolic engineering and process optimization
Yufei Zhang, Junhua Yun, Hossain M. Zabed, Yuan Dou, Guoyan Zhang, Mei Zhao, Mohammad J. Taherzadeh, Arthur Ragauskas, and Xianghui Qi
Elsevier BV



Enhancing the antibacterial activity of Lactobacillus reuteri against Escherichia coli by random mutagenesis and delineating its mechanism
Enyun Ma, Yingfeng An, Guoyan Zhang, Mei Zhao, Muhammad Waheed Iqbal, Hossain M. Zabed, and Xianghui Qi
Elsevier BV



Active polyhydroxybutyrate (PHB)/sugarcane bagasse fiber-based anti-microbial green composite: material characterization and degradation studies
Poorna Chandrika Sabapathy, Sabarinathan Devaraj, Parthiban Anburajan, Amreesh Parvez, Preethi Kathirvel, and Xianghui Qi
Springer Science and Business Media LLC



Sustainable biosynthesis of 3-hydroxypropionic acid from crude glycerol: Metabolic engineering and process optimization
Yufei Zhang, Junhua Yun, Lei Zhou, Guoyang Zhang, Mei Zhao, Hossain M. Zabed, and Xianghui Qi
Elsevier BV



Efficient caproate production from ethanol and acetate in open culture system through reinforcement of chain elongation process
Cunsheng Zhang, Zhihui Ling, Li Yang, Yan Liu, Tianyu Cao, Yuxuan Sun, Weimin Liu, Shuhao Huo, Zhi-Hong Zhang, Haijia Su,et al.
Elsevier BV



Editorial: Microorganism and process engineering for biosynthesis
Xianghui Qi, Jiandong Cui, M. Mofijur, and Hossain M. Zabed
Frontiers Media SA
School of Life Sciences, Guangzhou University, Guangzhou, Guangdong, China, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China, State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin Economic and Technological Development Area (TEDA), Tianjin, China, Faculty of Engineering and IT, University of Technology Sydney, Sydney, NSW, Australia, Mechanical Engineering Department, Prince Mohammad Bin Fahd University, Al Khobar, Saudi Arabia