Radoslaw Kajetan Kowalski
@pan.olsztyn.pl
Department of Gamete and Embryo Biology
Institute of Animal Reproduction and Food Research
RESEARCH, TEACHING, or OTHER INTERESTS
Aquatic Science, Animal Science and Zoology, Physiology, General Environmental Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Agnieszka Brzyszcz, Sylwia Judycka, Katarzyna Dryl, Beata I. Cejko, Beata Sarosiek, and Radosław K. Kowalski
Elsevier BV
Viktoriya Dzyuba, Joanna Giebułtowicz, Borys Dzyuba, Ganna Fedorova, Vitaliy Kholodnyy, Radosław Kajetan Kowalski, Agnieszka Brzyszcz, and Sergii Boryshpolets
Elsevier BV
D. Fopp-Bayat, A. Nitkiewicz, B. Sarosiek, and R.K. Kowalski
Elsevier BV
Beata Irena Cejko, Sławomir Krejszeff, Radosław Kajetan Kowalski, and Sylwia Judycka
Elsevier BV
Beata Irena Cejko, Katarzyna Dryl, Beata Sarosiek, Jarosław Ilgert, Marcin Jesiołowski, and Radosław Kajetan Kowalski
Elsevier BV
Beata Sarosiek, Katarzyna Dryl, Radosław K. Kowalski, Katarzyna Palińska-Żarska, and Daniel Żarski
MDPI AG
Although methods for the artificial reproduction of perch have been developed, a lack of information remains regarding the enzymes present in its semen, as well as their role in the fertilisation process. In this study, we first select the optimal activating solution for perch fertilisation and then determine the inhibition effect of enzymes that have already been reported as present in the sperm of teleosts—acid phosphatase (AcP), lactic dehydrogenase (LDH) and β-N-acetylglucosaminidase (β-NAGase)—on the percentage of motile spermatozoa and fertilised eggs. Of the 8 studied activation media, a solution composed of 80 mM NaCl, 20 mM KCl, 10 mM Tris, with pH 8.0 and 206 mOsm/kg proved to be optimal for perch gametes. The addition of ammonium molybdate (AcP inhibitor) caused no significant reduction in the percentage of fertilised eggs. On the other hand, the addition of 0.25 mM gossypol (LDH inhibitor) and 0.125 M acetamide (β-N-acetylglucosaminidase inhibitor) significantly decreased the fertilisation percentage to 41.1% and 52.4%, respectively, in contrast to the control (89.9 %). Both LDH and β-NAGase thus seem to play a very important role in the perch fertilisation process.
Beata Irena Cejko, Daniel Żarski, Beata Sarosiek, Katarzyna Dryl, Katarzyna Palińska-Żarska, Wojciech Skorupa, and Radosław Kajetan Kowalski
Elsevier BV
Agata Kowalska, Maciej Kamaszewski, Marta Czarnowska-Kujawska, Piotr Podlasz, and Radosław K. Kowalski
MDPI AG
A 3-week feeding trial was conducted in medaka broodstock (age five months) to examine the effect of dietary arachidonic acid (ARA) level (range: 4–23 mg g−1 of total fatty acids (TFAs)) on fertility, cyclooxygenase (COX) activity, egg size, sperm motility parameters, hatching rate and weight of hatch, survival and growth of larvae. After spawn induction and dietary exposure to 4 mg ARA g−1 TFA, broodstock were fed a diet containing ARA in the amounts: 4 (continued, as control), 5, 14 and 23 mg g−1 TFA. COX1 activity in the liver and the number of COX2-positive cells in the ovaries was increased in females fed the diets containing the two highest amounts of ARA. The highest sperm motility parameters were observed in males fed a diet containing 23 mg ARA g−1 TFA. The hatchability rate and bodyweight of hatchlings were higher in the group obtained from broodstock fed a diet containing 23 mg ARA g−1 TFA (79% and 0.66 mg fish−1, respectively) compared with 4 mg ARA g−1 TFA (50% and 0.40 mg fish−1). The average mortality of offspring obtained from this group at 7 days post hatching was significantly higher than that of all other groups.
Radosław Kajetan Kowalski, Beata Irena Cejko, Joanna Grudniewska, Stefan Dobosz, Mirosław Szczepkowski, and Beata Sarosiek
MDPI AG
We describe the technique of pneumatic stripping of whitefish (Coregonus lavaretus) eggs with the use of oxygen, nitrogen, and air. Eggs obtained via the traditional method (by pressing the abdominal surfaces) served as a control group. It was established that the gas flow rate during pneumatic stripping should not exceed 0.5 L∙min−1, since higher air flow resulted in increased post-spawning mortality. The pneumatic stripping method of egg collection was no faster than hand stripping; however, the time required per female was more consistent. It was found that the pH of the ovarian fluid obtained during hand and pneumatic stripping was not related to the success rate of fertilization. Pneumatic stripping resulted in a higher quality of collected eggs and a higher and more consistent hatching rate as compared with the hand-stripped samples, regardless of the gas used. The results presented here lead us to recommend the pneumatic method for obtaining eggs from whitefish, since it is a simple, reproducible method and improves the reproductive performance and developmental success of the fish eggs.
Beata Irena Cejko, Beata Sarosiek, Katarzyna Dryl, Sylwia Judycka, Bożena Szczepkowska, Mirosław Szczepkowski, and Radosław Kajetan Kowalski
Elsevier BV
Radosław Kajetan Kowalski and Beata Irena Cejko
Elsevier BV
Beata Irena Cejko, Daniel Żarski, Katarzyna Palińska-Żarska, Mariola Słowińska, and Radosław Kajetan Kowalski
Elsevier BV
Stanley Ifeanyi Ugwu, Agata Kowalska, Masaya Morita, and Radoslaw Kajetan Kowalski
Central Fisheries Research Institute (SUMAE)
Beata Irena Cejko, Ákos Horváth, Timea Kollár, Eszter Kása, Jelena Lujić, Zoran Marinović, Béla Urbányi, and Radosław Kajetan Kowalski
Springer Science and Business Media LLC
Radosław Kajetan Kowalski, Beata Sarosiek, Sylwia Judycka, Katarzyna Dryl, Joanna Grudniewska, Stefan Dobosz, and Beata Irena Cejko
MyJove Corporation
Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on fish abdomens to expel the eggs. An alternative method uses the pressure of gas injected into the body cavity, which causes the subsequent release of the eggs. This method is believed to have less negative effects on both the welfare and egg quality of the broodstocks. Herein, we compare the results of air and hand stripping methods with respect to one-year survival and egg quantity and quality in two salmonid fish, rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta morpha fario). Our results indicate that air stripping yielded a better quality of eggs and higher one-year survival rate in rainbow trout. In addition, air stripping resulted in lower mortality rate than the group subjected to hand stripping (25% vs. 35%). The pH and hatching rate of the hand stripped group was lower than those of the air stripped group. In the case of brown trout, the quality of eggs obtained by both hand and air-stripping methods was similar; however, the one-year losses in fish were higher in air stripped group (15% compared to 0% in hand stripped fish). Although the advantages of air stripping method over hand stripping in terms of egg quality might not be observed in all salmonid species, the air-stripping procedure might be a promising option to be adopted in hatcheries as it ensures a high level of reproducibility and efficiency.
Beata Irena Cejko, Beata Sarosiek, Sławomir Krejszeff, and Radosław Kajetan Kowalski
Elsevier BV
Beata Irena Cejko, Mariola Słowińska, Sylwia Judycka, and Radosław Kajetan Kowalski
Elsevier BV
Beata Irena Cejko, Sylwia Judycka, Dorota Juchno, Alicja Boroń, Anna Leska, Olga Jabłońska, Anna Pecio, and Radosław Kajetan Kowalski
Hindawi Limited
Agata Kowalska, Andrzej K. Siwicki, and Radosław K. Kowalski
Springer Science and Business Media LLC
Dorota Juchno, Anna Pecio, Alicja Boroń, Anna Leska, Olga Jablonska, Beata Irena Cejko, Radosław Kajetan Kowalski, Sylwia Judycka, and Mirosław Przybylski
Wiley
AbstractThe diploid–polyploid populations of Cobitis distributed in Poland are usually composed of the spined loach Cobitis taenia or, less often, the Danubian loach C. elongatoides and their triploid (females) and tetraploid hybrids (females and males). The aim of this study was to determine whether tetraploid males participate in the reproduction process by analyzing their testis ultrastructure and the process of spermatogenesis in comparison with diploid males of both parental species. Tetraploid loaches were obtained from three different diploid–polyploid populations distributed in Poland. The structure of Cobitis testes are typical for most Teleostei fish with cystic‐type spermatogenesis. The successive stages of developing germ cells are enclosed within cysts formed by the Sertoli cells. This paper morphologically describes the different germ cell stages of spermatogenesis (spermatogonia, spermatocytes, spermatids, and spermatozoa) of C. taenia and C. elongatoides and provides a pioneering ultrastructural analysis of tetraploid Cobitis testes which reveals their unusual structure for the first time. Thus, cysts with normal spermatogonia and spermatocytes (pachyten or leptoten stages) containing synaptonemal complexes were present and no spermatids or spermatozoa were observed. Moreover, in contrast to previously analyzed diploid species, single cells or all of the cells within the cysts displayed chromatin condensation and/or chromatin fragmentation. The obtained results clearly demonstrated that tetraploid males are sterile and diploids are fertile and are the only sperm donors in the reproduction processes of diploid–polyploid Cobitis populations.
Oliwia Michalik, Radosław Kowalski, Elżbieta Ziomek, Piotr Hliwa, Agata Mieszkowska, Rafał Rożyński, and Konrad Ocalewicz
Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
In species like rainbow trout (Oncorhynchus mykiss) that show male heterogamety (XY), induced androgenesis results in production of females (XX) and males (YY). Morphological and genetic differences between rainbow trout X and Y chromosomes suggest that androgenetic males (YY) may show lower survival than androgenetic females (XX). To test this hypothesis, we produced lines of androgenetic rainbow trout composed of males (YY) and females (XX) and of only females (XX) using spermatozoa from normal males (XY) and neo-males (XX), respectively. Survival rates of the androgenotes were monitored until gonadal differentiation and no significant differences in survival between XX/YY and XX androgenetic rainbow trout lines were observed. Sex-genotypes of the paternal males and androgenetic offspring were confirmed using a rainbow trout Y chromosome-related DNA marker. Histological analysis performed to detect potential disturbances in the gonadal development of the androgenetic XX and YY rainbow trout showed proper development of testes and ovaries in most of the studied individuals. Our results indicated that differences between rainbow trout X and Y chromosomes did not affect survival of the androgenetic offspring. Application of spermatozoa from neo-males to induce androgenetic development may have some potential in rainbow trout aquaculture to produce homozygous females that may be used in breeding programmes and to establish clonal lines.
S. Judycka, B.I. Cejko, K. Dryl, S. Dobosz, J. Grudniewska, and R.K. Kowalski
Elsevier BV
Beata Sarosiek, Katarzyna Dryl, Sylwia Judycka, Stefan Dobosz, Joanna Grudniewska, and Radosław K Kowalski
Hindawi Limited
Oliwia Michalik, Radosław K. Kowalski, Sylwia Judycka, Rafał Rożyński, Stefan Dobosz, and Konrad Ocalewicz
Elsevier BV
B. I. Cejko, M. Słowińska, S. Judycka, and R. K. Kowalski
Wiley
Substrate specificity in the seminal plasma and testes fluids of the common carp Cyprinus carpio was determined using gelatin, casein, albumin and haemoglobin. Proteolytic profiles of the testes and seminal plasma were compared. Different ranges of pH (5·5–9·5) and temperature (4–37° C) were used during incubations of seminal plasma proteinases. Differences in proteolytic activity between testes and seminal plasma may reflect specific functions of the testes and sperm ducts in semen production. Seminal plasma metalloproteinases were characterized by higher substrate specificity than were serine proteinases. Zymography optimization for seminal plasma indicated that pH 7·5 and 22° C were the optimal conditions for gel incubations.