Plant Response to Bacterial Infections: miRNAomics Approach Sumira Malik, Shristi Kishore, Nitesh Singh, Rahul Kumar Mirnaomics and Stress Management in Plants, 2024 Plant microRNAs (miRNAs) have a significant role in plant disease resistance through the elicitation of a defence mechanism. Plants are most infected by many bacterial pathogens, in response to which they regulate the expression of genes through the mechanism of activation and repression of the vast array of genes. The endogenous small RNAs (sRNAs) consist of key role player microRNAs (miRNAs) that post-transcriptionally perform RNA silencing and mediate gene regulation in response to such bacterial infections in plant-host interaction. Recently, numerous omics-based approaches and studies have unrevealed the function of plant miRNAs during bacterial pathogen infection and invasion. The current chapter provides highlights of research findings demonstrating the plant miRNAs’ active involvement in and against bacterial infection through a multi-omics-oriented approach.
Tolerance to Radiation Stress in Plants With Reference to microRNAs Sumira Malik, Shilpa Prasad, Rahul Kumar, Shristi Kishore, Nitesh Singh Mirnaomics and Stress Management in Plants, 2024 In a natural habitat, a plant encounters several abiotic and biotic factors that are responsible for affecting its growth and development. The abiotic stress is composed of factors such as ultraviolet (UV) radiation, temperature variance, high salinity, water availability (either low or high), heavy metals, etc. Biotic stress is caused due to several biotic agents such as bacteria, fungi, etc. Radiation stress is one of the abiotic factors that can affect plant physiology, and to combat this situation, the plant needs to be tolerant to radiation stress. Light intensity and quality are important aspects of radiation and can cause deleterious effects if not provided within the correct parameter. The biosphere receives solar radiations at different wavelengths, and if photosynthetically active radiations, that is, PAR (400–700 nal), are provided in more amounts, then it can cause increased absorption of UV-radiations, thereby leading to radiation stress in plants. Radiation stress causes severe losses to the production of agricultural and commercial plants. Recent developments in molecular biology have paved the way to increase plant tolerance against radiation stress using miRNAs (micro RNAs). These miRNAs are small non-coding RNAs that consist of 20–24 nucleotides in length and can also regulate gene expression. The miRNAs are important contributors to plant stress response, thus by identifying the appropriate miRNA/s and their target gene, the tolerance level of plants against radiation stress can be modulated. The present chapter will focus on several studies related to the application of miRNAs in enhancing and managing plant tolerance against radiation stress.
Chitosan as a Growth Promoter and Enhance Survival Rate in an in vitro Culture of Banana (Musa spp.) Cultivar ‘Bantala’ Laxman Kandha, Rahul Kumar, Birendra Kumar Bindhani Indian Journal of Agricultural Research, 2022 Background: Banana (Musa spp.) is one of the most consumable fruits and cultivated around the globe. It contains high nutritional value as well as the high demand of the market. The microbes are the main problem for the propagation of banana plants in tissue culture. Chitosan is one of the best substances for the eradication of contamination and also growth stimulators of banana plants. This study is based on the micro-propagation of the bantala variety of Musa species and free from microbe infection. Methods: The rhizome and sucker as explants of Musa cv. Bantala. The different combination concentrations of 6-Benzylaminopurine (BAP), indole-3-acetic acid (IAA) and chitosan (CS) were tried in Murashige and Skoog medium for in vitro response of plants, shoot initiation and shoot proliferation. The formation of rooting was used as the half-strength Murashige and Skoog (MS) medium with indole-3-butyric acid (IBA) and chitosan (CS). Result: The best response, shoot initiation and shoot proliferation were observed at 6-Benzylaminopurine (5.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chitosan (25 mg/L) and 6-Benzylaminopurine (4.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chatoyant (25 mg/L) in both of rhizome and sucker respectively. The maximum root formations were observed in the medium containing half-strength Murashige and Skoog medium+1.0 mg/L indole-3-butyric acid+25 mg/L chitosan in the rhizome and 0.8 mg/L indole-3-butyric acid+25 mg/L chitosan in the sucker. The successful survival rate of sucker and rhizome under the acclimatization condition was recorded as 90% and 88% compared with control as 66% and 63% respectively. This standardized protocol might be useful for the mass production of bantala variety as well as other cultivars of banana plants.
Nutraceuticals: An Alternative of Medicine Rahul Kumar, Sourav Das, Santosh Kumar Sethi, Pankaj Kumar Parhi, Birendra Kumar Bindhani Prebiotics Probiotics and Nutraceuticals, 2022
Chitosan enhances growth and survival rate of in vitro-cultured plantlets of banana cultivar “Grand Naine” Laxman Kandha, Rahul Kumar, Santosh Kumar Sethi, Birendra Kumar Bindhani Journal of Crop Improvement, 2021 Banana (Musa spp.) is one of the most popular fruits consumed worldwide. Multiplication of banana through tissue culture is faced with the problem of fungal contaminations. Shrimp chitosan reportedly enhances growth in tissue-cultured plants and reduces fungal and bacterial infections during the in-vitro culture of plants. The present investigation was conducted to establish an in-vitro propagation protocol with zero fungal and bacterial contamination of cultivar ‘Grand Naine’ using explants from the rhizome and sucker. Different combinations of 6-benzylamino purine and indole-3-acetic acid were tested in Murashige and Skoog medium to validate the influence of phytohormones on the response of explants and regeneration of shoots and roots in it. The best response and regeneration were observed at BAP 5.0 mg/L and IAA 0.5 mg/L for the rhizome explants and BAP 4.0 mg/L and IAA 0.5 mg/L for the sucker explants. The best root formation was observed at IBA 0.8 mg/L and 1.0 mg/L for the rhizome and sucker explants, respectively. To validate the effect of shrimp chitosan on the growth of both explants, culture media was supplemented with chitosan in different concentrations. The best growth of banana explants was observed with 25 mg/L shrimp chitosan. The plantlets were hardened successfully in the greenhouse, with a survival rate of 93% for the sucker and 91% for the rhizome explants; compared with 68% and 65% for both positive controls, respectively. The present standardized protocol should be useful for the mass production of banana, germplasm conservation and development of economically important banana cultivars. Abbreviations: BAP: 6-Benzylamino purine; CS: Chitosan; DPI: Days post inoculation; IAA: Indole-3-acetic acid; IBA: Indole-3-butyric acid; MS: Murashige and Skoog medium; MT: Metric ton; Ha: Hectare; mg/L: Milligram per liter
Nyctanthes arbor-tristis mediated synthesis of novel chitosan coated gold nanoparticles: Their stability and anti-bacterial activities Research Journal of Chemistry and Environment, 2020
Plant Resilience to Abiotic Stress: Enhancing Mechanisms, Strategies, and Innovations RK Vasudha Maurya Journal of Plant Growth Regulation , 2026 2026 Citations: 1
Emerging Antimicrobial Resistance in Burn Wound Infections: Analysis from a Burn ICU in a Tertiary Care Hospital S Rout, R Kumar, KK Sahu Research and Innovations in Industrial and Marine Biotechnology: A Circular … , 2026 2026
Microalgal EPS as a bio-flocculant for sustainable biomass harvesting: A review H Nishad, S Kashyap, A Khan, R Kumar, RK Mohapatra, M Nayak Separation and Purification Technology, 134844 , 2025 2025 Citations: 4
Emerging antimicrobial resistance in burn wound infections: Analysis from a burn ICU in tertiary care hospital SKK Rout S., Kumar, R. Research and Innovations in Industrial and Marine Biotechnology: A Circular … , 2025 2025
Evaluation of burn wound microbes and their antibiotic susceptibility pattern SKK Rout S., Kumar, R., Kar S. BioMedicine 45 (1), 48-56 , 2025 2025
Molecular identification of Pseudomonas aeruginosa isolated from burn patients by polymerase chain reaction in central Odisha, India S Rout, R Kumar, KK Sahu Journal of Integrative Medicine and Research 3 (2), 109-116 , 2025 2025
Temporal dynamics of the burn wound microbiome and their resistance towards various antibiotics: A hospital‑based observational study RSKK Rout, S., Kumar Journal of Integrative Medicine and Research 3 (1), 24-29 , 2025 2025 Citations: 1
Laboratory Techniques in Microbiology R Rout, S., Kumar 2025
Mineral Nutrition of Plants R Kumar, V., Kumar 2025
Plant Response to Bacterial Infections: miRNAomics Approach S Malik, S Kishore, N Singh, R Kumar miRNAomics and Stress Management in Plants, 119-126 , 2024 2024
Tolerance to Radiation Stress in Plants With Reference to microRNAs S Malik, S Prasad, R Kumar, S Kishore, N Singh miRNAomics and Stress Management in Plants, 88-96 , 2024 2024
The Current State of Art and Future Prospects RK Sourav Das, Saikat Ghosh, Abishek Bakshi, Shweta Khanna, Birendra Kumar ... Biological Applications of Nanoparticles, 101-120 , 2023 2023
Emphasizes the Role of Nanotechnology in Bioremediation of Pollutants SMRK Saikat Ghosh, Priyanka Dheer, Nilaya Kumar Panda, Soumya Biswas, Sourav ... Industrial Wastewater Reuse, 469–504 , 2023 2023 Citations: 6
Prebiotics, Probiotics and Nutraceuticals KR Bindhani, B. K., Das, S., Sethi, S. K., Parhi, P. K. Springer , 2022 2022 Citations: 10
Chitosan as a growth promoter and enhance survival rate in an in-vitro culture of banana (Musa spp.) cultivar ‘Bantala’ BK Kandha, L., Kumar, R.* & Bindhani Indian Journal of Agricultural Research 56 (5), 599-606 , 2022 2022 Citations: 3
Chitosan enhances growth and survival rate during in-vitro culture of banana (Musa spp.) cultivar Grand Naine. BK Kandha, L., Kumar, R., Hembram, K. C., Bindhani Journal of Crop Improvement 35 (6), 848-865 , 2021 2021 Citations: 13
Nyctanthesarbor-tristis mediated synthesis of novel chitosan coated gold nanoparticles: their stability and anti-bacterial activity BK Kumari, K., Hembram, K. C., Kandha, L., Kumar, R.* &Bindhani Research Journal of Chemistry and Environment 24 (4), 85-91 , 2020 2020
Chitosan-based in-vitro propagation of banana (Cv. Grand Naine) and its genetic uniformity assessment BBK Kandha, L., Kumar, R., Hembram, K. C. Research Journal of Biotechnology 14 (12), 22-29 , 2019 2019 Citations: 1
Expression of an endo α-1, 3-Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight R Kumar, K Kumari, KC Hembram, L Kandha, BK Bindhani Journal of plant biochemistry and biotechnology 28 (1), 84-90 , 2019 2019 Citations: 27
Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani Artificial cells, nanomedicine, and biotechnology 46 (sup3), S38-S51 , 2018 2018 Citations: 184
MOST CITED SCHOLAR PUBLICATIONS
Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani Artificial cells, nanomedicine, and biotechnology 46 (sup3), S38-S51 , 2018 2018 Citations: 184
Expression of an endo α-1, 3-Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight R Kumar, K Kumari, KC Hembram, L Kandha, BK Bindhani Journal of plant biochemistry and biotechnology 28 (1), 84-90 , 2019 2019 Citations: 27
Chitosan enhances growth and survival rate during in-vitro culture of banana (Musa spp.) cultivar Grand Naine. BK Kandha, L., Kumar, R., Hembram, K. C., Bindhani Journal of Crop Improvement 35 (6), 848-865 , 2021 2021 Citations: 13
Prebiotics, Probiotics and Nutraceuticals KR Bindhani, B. K., Das, S., Sethi, S. K., Parhi, P. K. Springer , 2022 2022 Citations: 10
Emphasizes the Role of Nanotechnology in Bioremediation of Pollutants SMRK Saikat Ghosh, Priyanka Dheer, Nilaya Kumar Panda, Soumya Biswas, Sourav ... Industrial Wastewater Reuse, 469–504 , 2023 2023 Citations: 6
Microalgal EPS as a bio-flocculant for sustainable biomass harvesting: A review H Nishad, S Kashyap, A Khan, R Kumar, RK Mohapatra, M Nayak Separation and Purification Technology, 134844 , 2025 2025 Citations: 4
Chitosan as a growth promoter and enhance survival rate in an in-vitro culture of banana (Musa spp.) cultivar ‘Bantala’ BK Kandha, L., Kumar, R.* & Bindhani Indian Journal of Agricultural Research 56 (5), 599-606 , 2022 2022 Citations: 3
Plant Resilience to Abiotic Stress: Enhancing Mechanisms, Strategies, and Innovations RK Vasudha Maurya Journal of Plant Growth Regulation , 2026 2026 Citations: 1
Temporal dynamics of the burn wound microbiome and their resistance towards various antibiotics: A hospital‑based observational study RSKK Rout, S., Kumar Journal of Integrative Medicine and Research 3 (1), 24-29 , 2025 2025 Citations: 1
Chitosan-based in-vitro propagation of banana (Cv. Grand Naine) and its genetic uniformity assessment BBK Kandha, L., Kumar, R., Hembram, K. C. Research Journal of Biotechnology 14 (12), 22-29 , 2019 2019 Citations: 1
Emerging Antimicrobial Resistance in Burn Wound Infections: Analysis from a Burn ICU in a Tertiary Care Hospital S Rout, R Kumar, KK Sahu Research and Innovations in Industrial and Marine Biotechnology: A Circular … , 2026 2026
Emerging antimicrobial resistance in burn wound infections: Analysis from a burn ICU in tertiary care hospital SKK Rout S., Kumar, R. Research and Innovations in Industrial and Marine Biotechnology: A Circular … , 2025 2025
Evaluation of burn wound microbes and their antibiotic susceptibility pattern SKK Rout S., Kumar, R., Kar S. BioMedicine 45 (1), 48-56 , 2025 2025
Molecular identification of Pseudomonas aeruginosa isolated from burn patients by polymerase chain reaction in central Odisha, India S Rout, R Kumar, KK Sahu Journal of Integrative Medicine and Research 3 (2), 109-116 , 2025 2025
Laboratory Techniques in Microbiology R Rout, S., Kumar 2025
Mineral Nutrition of Plants R Kumar, V., Kumar 2025
Plant Response to Bacterial Infections: miRNAomics Approach S Malik, S Kishore, N Singh, R Kumar miRNAomics and Stress Management in Plants, 119-126 , 2024 2024
Tolerance to Radiation Stress in Plants With Reference to microRNAs S Malik, S Prasad, R Kumar, S Kishore, N Singh miRNAomics and Stress Management in Plants, 88-96 , 2024 2024
The Current State of Art and Future Prospects RK Sourav Das, Saikat Ghosh, Abishek Bakshi, Shweta Khanna, Birendra Kumar ... Biological Applications of Nanoparticles, 101-120 , 2023 2023
Nyctanthesarbor-tristis mediated synthesis of novel chitosan coated gold nanoparticles: their stability and anti-bacterial activity BK Kumari, K., Hembram, K. C., Kandha, L., Kumar, R.* &Bindhani Research Journal of Chemistry and Environment 24 (4), 85-91 , 2020 2020