Biotechnology, Chemical Engineering, Food Science, Biochemistry, Genetics and Molecular Biology
20
Scopus Publications
607
Scholar Citations
12
Scholar h-index
16
Scholar i10-index
Scopus Publications
Neural network based prediction of site-specific stable mutants in wild type CFTR protein Sudharshan Reddy Dachani, Rishikesh Shukla, Bharath Kumar Kakkireni, Shreyas Sadineni, Balla Sai Mrudula, et al. Indian Journal of Biochemistry and Biophysics, 2025 Cystic fibrosis is a genetic disorder characterized by defective ion transport across cell membranes, primarily affecting the lungs and other vital organs. The cystic fibrosis transmembrane conductance regulator (CFTR) protein plays a pivotal role in cystic fibrosis pathophysiology, and mutations in the CFTR gene lead to dysfunctional protein function. The CFTR modulators, including ivacaftor, lumacaftor, and tezacaftor, represent promising therapeutic options for improving CFTR function and ameliorating cystic fibrosis symptoms the CFTR (PDB ID: 5UAK) structure stability was assessed by replacing the phenylalanine with other amino acid at 508th position by using the ThermoMPNN tool. The stable mutant structure (F508W) was identified and its structure was generated using the Biopython library. In this study, we employed molecular docking to investigate the interactions between CFTR modulators and CFTR protein structures. For the wild type CFTR and F508W mutant counterpart, docking was performed with ivacaftor, lumacaftor, and tezacaftor using the CB-Dock2 platform. Our results indicate that lumacaftor exhibited the highest predicted binding affinity among the tested CFTR modulators for the F508W mutant CFTR, suggesting its potential as a therapeutic option for individuals with cystic fibrosis carrying this mutation. Additionally, interaction profile analyses revealed stable complexes formed between the CFTR modulators and both wild type and mutant CFTR structures, highlighting their potential effectiveness in targeting CFTR dysfunction. Overall, this study provides valuable insights into the molecular mechanisms underlying the therapeutic efficacy of CFTR modulators in cystic fibrosis. Further experimental validation is warranted to confirm these computational findings and to explore the clinical utility of these drugs in treating cystic fibrosis.
Synthesis of Small Molecule Library of Novel Nicotinamide Derivatives as Anticancer Agents and Computational Screening Ram Mohan Malothu, Gangadhar Thalari Chemistryselect, 2025 A small molecule library of nicotinamide derivatives synthesized and characterized their structure by interpretation of 1 H NMR, 13 C NMR, and Mass spectral data. The new molecules were screened for their in vitro anticancer activity against human breast (MCF‐7) and cervical (HeLa) cell lines by using Doxorubicin as standard reference. Pyridine linked nicotinamide derivatives displayed potent activity against both the cell lines. The trifluoromethyl substituted pyridine analogue demonstrated outstanding activity with IC 50 value of 8.70 ± 0.23 µM and 8.97 ± 0.31 µM against MCF‐7 and HeLa cell lines respectively, with respect to Doxorubicin IC 50 value of 9.06 ± 0.36 µM (MCF‐7) and 9.17 ± 0.39 µM (HeLa). The compound 6 with thiomethyl group displayed activity with IC 50 value of 9.01 ± 0.38 µM and 9.82 ± 0.41 µM against MCF‐7 and HeLa cell lines correspondingly. The methyl substituted compound 5h displayed encouraging activity with IC 50 value of 10.47 ± 0.41 µM (MCF‐7) and 11.87 ± 0.45 µM (HeLa). Molecular docking study performed using potent molecules 5h , 5i, and 6 against estrogen receptor alpha (PDB ID: 3ERT), obtained highest docking score value for ligand 5i (9.5 kcal/mol). The predicted ADME of compounds indicated their drug‐like characteristics.
Design, Synthesis, Cytotoxicity, and Molecular Docking of New Thiazole Linked Tetrahydropyridine: Pyridine Hybrids Ram Mohan Malothu, Gangadhar Thalari Polycyclic Aromatic Compounds, 2025 Cancer is a serious health issue that has affected people of all ages and everywhere for a long time. In view of it a library of thiazole—tetrahydropyridine—pyridine derivatives synthesized evaluated for their in vitro anticancer activity against human breast adenocarcinoma (MCF-7) and human lung cancer (A-549) cell lines, using Doxorubicin as the standard reference. The compound 6k with -F and -CH3 substituents found to be more potent with IC50 value of 8.72 ± 0.88 µM and 8.54 ± 0.85 µM against MCF-7 and A-549 cells, respectively, compound 6c containing trifluoromethoxy group as substituent with IC50 value of 9.45 ± 1.02 µM (MCF-7) and 9.59 ± 1.08 µM (A-549), compound 6 g with trifluoromethyl and methyl substituents presented prominent activity with IC50 value of 11.19 ± 1.03 µM (MCF-7) and 11.34 ± 1.07 µM (A-549) and compound 6 m with trifluoromethyl group showed good activity with IC50 value of 14.54 ± 1.03 µM (MCF-7) and 14.35 ± 1.05 µM (A-549). The toxicity test against Hek-293 revealed that they are not harmful to normal cells. Molecular docking study of potent ligand 6k revealed their best dock score and promising binding interactions. Presented ADME prediction of all compounds.
Recent Advances in engineering Corynebacterium glutamicum for production of Lysine Divya Kakara, Malothu Ramesh, Dowlathabad Muralidhara Rao, Suman Dheerendra Kumar, Erva Rajeswara Reddy Research Journal of Biotechnology, 2022 Lysine is an essential amino acid utilised in diverse industrial areas, mostly as animal feed and food. It is synthesized by various organisms but the novel Corynebacterium glutamicum (gram positive, non sporulating bacteria) is selected anticipating to the enormous research. This research has paved a way towards enhancing the lysine productivity. Different strains of Corynebacterium glutamicum utilised many technologies for the enhanced production of L-Lysine. This literature study examines the current status of L-lysine industrial production with a focus on technology used for development irrespective of the strain and fermentation technologies through different approach of understanding of provisions (NADPH, Media source, PTS, exploit of RAM, Vector systems and promoters) with use of different methodologies. The technologies were divided into metabolic engineering, conventional gene mutations, optimization of fermentation parameters (RSM, ANN, GA), gene overexpression, gene switching and exploit of CRISPR, electric current. This review presents the methodologies which are superior at manufacturing enhanced amounts of lysine and report the techniques which assist in producing the best yield in collation with the earlier. This also focuses on the genes related to lysine export and provides conviction of utilizing lysine as an animal feed.
Structural determination of l-asparaginase ii of streptomyces albidoflavus and interaction analysis with l-asparagine and cefotaxime ACHYUTUNI VENKATA NAGA TEJASWINI, MALOTHU RAMESH International Journal of Applied Pharmaceutics, 2021 Objective: L-Asparaginase enzyme possesses a crucial role in the treatment of various hematologic malignancies. The current study focuses on homology modeling and interaction analysis of L-Asparaginase proteins belonging to Streptomyces albidoflavus (S. albidoflavus) with the essential ligand L-Asparagine and subsequent analysis with essential β-lactam antibiotic Cefotaxime.
 Methods: The process of understanding Asparaginase interactions primarily involved structure determination of WP_096097608, WP_095730301, which is achieved by GalaxyTBM, I-TASSER and SWISS-MODEL. Further, the S. albidoflavus Asparaginase proteins are subjected to GalaxySite and Autodock Vina of PyRx analysis.
 Results: The GalaxyTBM predicted structures of both the proteins are found promising on various validation studies. The two Asparaginase proteins exhibited high binding affinities of-6.8 and-6.5 kcal/mol with Cefotaxime and-5.1 and-4.9 kcal/mol towards Asparagine. The protein WP_096097608 residues forming hydrogen bonds with L-Asparagine are also analysed to involve in interaction with Cefotaxime on individual docking analysis.
 Conclusion: The current findings details the two S. albidoflavus Asparaginase proteins affinity towards L-Asparagine, hence can be assessed further for immunogenicity studies. In addition to the above findings, an attempt is made to find the L-Asparaginase binding possibilities with non-metals that identified an essential β-lactam antibiotic Cefotaxime to be an effective inhibitor. This study helps in understanding the interactions of L-Asparaginase with Cefotaxime, as intake of antibiotics between the phases of chemotherapy is observed to treat various infections and also as an antibiotic to microbes that utilize Asparaginase as a vital enzyme.
Electroencephalogram correlates the role of ashwagandha in sleep and memory promoting effect Mounika Basani, Ramesh Malothu, Rakesh Kumar Sinha, Sindgi Vasudeva Murthy Indian Journal of Pharmaceutical Education and Research, 2021 Background: Ashwagandha (Withania somnifera), a known traditional medicine used in Ayurveda system of medicine, native to the India. Poor quality sleep always looked as serious complaint as it disrupts sleep. The natural phytoconstituents present in the ashwagandha believed to be adaptogen that helps the body to adapt stress by normalizing or correcting through balancing immune and neuroendocrine system. The mechanism that enhances sleep quality in the presence of ashwagandha is still unknown. Based on all available studies we investigated the effects of hydroalcoholic extract of ashwagandha effects on various bands of the electroencephalogram (EEG) to ascertain its role in behavior and sleep in rats. Material and methods: The present study explored in Wistar rats of either sex (n=12) used to record EEG in the presence of ashwagandha extracts (25mg/kg body weight). The previous studies revealed sleep promoting character of GABA very well documented in governing sleep and Withania somnifera known to posses mild to moderate hypnotic activity. We used conventional model for quantifying EEG in presence of ashwagandha extract and investigated its possible role in memory, behavior and sleep. Withania somnifera extract administered orally (25mg/ kg) for electrophysiological recordings. Comparison made between post administration of extract with baseline recording employing vehicle and is considered as control. The changes in various bands of EEG noticed following administration of ashwagandha extract. Results: The Withania somnifera extract increased electrophysiological properties in delta (p<0.001) and gamma (p<0.003) bands and was found to be statistically significant. Statistically significant changes not observed in theta and alpha band power but beta band power was reduced in its influence (p<0.013). Conclusion: The present study revealed enhanced activity of the slow wave in presence of ashwagandha extract and divulged the details of its role in sleep and memory.
Bioprocess optimization of L-lysine production by using RSM and artificial neural networks from Corynebacterium glutamicum ATCC13032 Vanasi Bhushanam, Ramesh Malothu Chemical Product and Process Modeling, 2020 L-Lysine is one of the important amino acid required for humans and animals. It has a high commercial market. Large scale production of this amino acid is essential to meet the commercial demands. Typically, L-lysine is produced by batch fermentation. In the present study, the important process, as well as nutrient parameters such as glucose concentration (g/L), rpm, incubation temperature (°C), pH and incubation time for L-lysine production by Corynebacterium glutamicum ATCC13032, were optimized by a combined approach of response surface methodology (RSM) with artificial neural network (ANN) method. Initially, 32 runs face central composite design was employed. In the first step, the data was analyzed by the RSM and the optimum conditions for L-lysine production were determined. In the second step, the same data was used to train the neural network. A feed-forward neural network with error backpropagation was used. The best network was obtained by optimizing the no of neurons in the hidden layer. From the best network, the optimized weights and predicted responses were used to optimize the conditions of the selected parameters by genetic algorithm (GA). Overall with the combination of RSM-ANN-GA onefold of L-lysine production from Corynebacterium glutamicum ATCC 13032 was improved.
Variation of protein concentration on diabetes affected people in Andhra Pradesh International Journal of Scientific and Technology Research, 2019
Improvement in the production of L-Lysne by ENU treated chemical mutagenesis of ddh gene recombinant strain of corynebacterium glutamicum MTCC25069 Current Trends in Biotechnology and Pharmacy, 2019
E2F1: Transcriptional machinery in colon cancer I. Vasavi, A. M. V. N. Prathyusha, Malothu Ramesh, K. Satish Kumar, B. Pratap Naidu, Pallaval Veera Bramhachari, N. M. Yugandhar, P. Rama Chandra Ganesh Role of Transcription Factors in Gastrointestinal Malignancies, 2018
Purification and characterization of protease enzyme from native isolate Bacillus subtilis and its compatability with commercial detergents Biotechnology an Indian Journal, 2013
Assays for oxidative stress and antioxidant status Research Journal of Pharmaceutical Biological and Chemical Sciences, 2013
Anti-hyperglycemic activity of the leaves of Terminalia tomentosa against normal and alloxan induced diabetic rats Research Journal of Pharmacy and Technology, 2012
RECENT SCHOLAR PUBLICATIONS
In silico structure prediction of Maturase K Protein of Annona muricata from its amino acid sequence using AI guided 3D structure prediction tool -AlphaFold and … TK Ramesh Malothu Research Journal of Biotechnology 21 (3), 44-50 , 2026 2026
Biodiesel Production from Chlorella Vulgaris by Media Optimisation and Transesterification D Kakara International journal of engineering research and technology , 2025 2025 Citations: 1
Neural network based prediction of site-specific stable mutants in wild type CFTR protein SR Dachani, R Shukla, BK Kakkireni, S Sadineni, BS Mrudula, R Malothu, ... Indian Journal of Biochemistry and Biophysics (IJBB) 62 (5), 511-517 , 2025 2025
A Review of AI Based Classification of Medicinal Plant using VGG-19 Technique P Krishnamoorthy, T Prasanth, VP Ganesh, S Rahul, M Ramesh IJSAT-International Journal on Science and Technology 16 (1) , 2025 2025 Citations: 1
Emerging Eco-Friendly Alternatives to SFâ ‚† for High Voltage Insulation: A Review SP Kruthi, M Ramesh, R Prasad International Journal of Research and Innovation in Applied Science 10 (7 … , 2025 2025
Study on induction of Allium sativum L. hairy roots with antibacterial activity against Vibrio VTB Phuong, NP Anh, NPQ Thang, T Phi, QND Phuong Res. J. Biotechnol. 18, 8 , 2023 2023 Citations: 6
Value added products generation from sugarcane bagasse and its impact on economizing biorefinery and sustainability of sugarcane industry R Korasapati, SB Basthipati, K Rosangzuala, PR Reddy, R Gajjala, ... Sugarcane-Its Products and Sustainability , 2023 2023 Citations: 10
Chlorella vulgaris, a fortune for the Biodiesel and Fatty Acid Production. KD Ramesh.M, Jaya Sahitya, Divya Bhanu Journal of Xi’an Shiyou University 19 (2), 347-359 , 2023 2023
Extraction of Biodiesel from Microalgae KD Ramesh.M, Srividya Journal of Xi’an Shiyou University 19 (3), 39-56 , 2023 2023
Genetic variability and divergence of morphological and seed quality traits of greengram (Vigna radiata L.) genotypes A Perala, R Malothu, S Patta, C Anuradha International Journal of Plant & Soil Science 34 (23), 1002-1011 , 2022 2022 Citations: 5
IMPLEMENTATION OF PMBLDC MOTOR DRIVEN ELECTRIC VEHICLE POWERED BY SOLAR MV RAMESH, RK MELIMI, TS RAO, PM KUMAR Journal of Theoretical and Applied Information Technology 100 (10) , 2022 2022
EVALUATION OF STRUCTURAL, MOLECULAR INTERACTION AND SIMULATION STUDY OF L-ASPARAGINASE OF Streptomyces koyangensis AVN Tejaswini, R Malothu Journal of Biochemistry International 9 (2), 1-13 , 2022 2022
Molecular Docking and MD Simulation Analysis of L-asparaginase of Streptomyces iranensis, Streptomyces himalayensis and Streptomyces griseus AVN Tejaswini, R Malothu Current Trends in Biotechnology and Pharmacy 15 (4), 390-400 , 2021 2021
Combination of Copper Bismuth Oxide (CuBi2O4) and Polymer Composites from Plastic Waste: A Boon for EMF Shielding R Malothu, RS Rao i-Manager's Journal on Future Engineering and Technology 16 (4), 11 , 2021 2021 Citations: 4
Effects of Electromagnetic Field Exposure and Evaluation of Biological Changes in Human Health D Ramu, R Malothu, RS Rao i-Manager's Journal on Electrical Engineering 14 (3), 1 , 2021 2021
Computer Aided Simulation to Improve Product Design Quality in Metal Casting Process G Gopi, DLK Rao, M Ramesh 2021
Electroencephalogram Correlates the Role of Ashwagandha in Sleep and Memory Promoting Effect M Basani, R Malothu, R Sinha, V Murthy Indian Journal of Pharmaceutical Education and Research 55 (1), 184-189 , 2021 2021 Citations: 3
Bioprocess Optimization of L-Lysine Production by Using RSM and Artificial Neural Networks from Corynebacterium glutamicum ATCC13032 V Bhushanam, R Malothu Chemical Product and Process Modeling 15 (4), 20190054 , 2020 2020 Citations: 10
Role of Orthoboon (glucosamine sulfate+ collagen+ Vitamin C): A novel host-modulating agent in the management of chronic periodontitis GS Penmetsa, A Mopidevi, V Ramaraju, R Ramachandran, MV Ramesh Journal of Indian Society of Periodontology 24 (5), 428-432 , 2020 2020 Citations: 2
BDNF is obverse to oxidative stress (adenosine deaminase and nitric oxide) in type II diabetes mellitus K Divya, R Malothu, EL Narayana International Journal of Diabetes in Developing Countries 40 (3), 390-398 , 2020 2020 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Medicinal uses of L-lysine: Past and future M Singh, DM Rao, S Pande, S Battu, K Mahalakshmi, KR Dutt, M Ramesh Int. J. Res. Pharm. Sci 2 (4), 637-642 , 2011 2011 Citations: 64
Effect of pranayama (rechaka, puraka and kumbaka) on bronchial asthma-an open study KRJ Murthy, BK Sahay, P Sitaramaraju, N Annapurna, M Ramesh Lung India 2 (2), 187-191 , 1984 1984 Citations: 44
Method development and validation of sitagliptin and metformin using reverse phase HPLC method in bulk and tablet dosage form SK Karimulla, PM Vasanth, T Ramesh, M Ramesh Der Pharmacia Lettre 5 (5), 168-174 , 2013 2013 Citations: 43
Green synthesis of gold nanoparticles using Mangifera indica (mango leaves) aqueous extract T Muralikrishna, R Malothu, M Pattanayak, PL Nayak World J. Nanosci. Technol 2, 66-73 , 2014 2014 Citations: 28
A Review on Biodegradable Packaging Materials in Extending the Shelf Life and Quality of Fresh Fruits and Vegetables GNSS M. Ramesh WasteManagementasEconomicIndustryTowardsCircularEconomy, 59-65 , 2020 2020 Citations: 27
Synthesis, characterization and anti-oxidant activity of Schiff base ligand and its metal complexes containing thiazole moiety M BHM, GY Nagesh, M Ramesh, B Priyanka, B Heena Der Pharma Chem 7, 556-62 , 2015 2015 Citations: 26
Characterization and wash performance analysis of microbial extracellular enzymes from East Calcutta Wetland in India R Oberoi American Journal of Applied Sciences , 2008 2008 Citations: 24
Therapeutic response of vitamin A, vitamin B complex, essential fatty acids (EFA) and vitamin E in the treatment of phrynoderma: a randomized controlled study S Ragunatha, SB Murugesh, M Ramesh, G Narendra Journal of Clinical and Diagnostic Research: JCDR 8 (1), 116 , 2013 2013 Citations: 20
Estimation of salivary parameters among autoimmune thyroiditis patients YA Syed, BHS Reddy, TK Ramamurthy, K Rajendra, NK Nerella, ... Journal of Clinical and Diagnostic Research: JCDR 11 (7), ZC01 , 2017 2017 Citations: 19
Neutralizing cross-site scripting attacks using open source technologies GRK Rao, RS Prasad, M Ramesh Proceedings of the Second International Conference on Information and … , 2016 2016 Citations: 15
Anti-hyperglycemic activity of the leaves of Terminalia tomentosa against normal and alloxan induced diabetic rats S Alladi, SD Prakash, R Malothu, M Nalini, SK Rao Research Journal of Pharmacy and Technology 5 (12), XI , 2012 2012 Citations: 14
A comparative evaluation of diode laser ablation versus scalpel excision for management of oral leukoplakia SA Yasmeen, BHS Reddy, MV Ramesh, SS Birajdar, A Yarram, ... Journal of Indian Academy of Oral Medicine and Radiology 31 (2), 94-99 , 2019 2019 Citations: 13
Formulation and evaluation of gastroretentive floating tablets of losartan potassium M Samyuktha, P Vasanth, K Suresh, T Ramesh, M Ramesh Int J Biopharm 4, 18-26 , 2013 2013 Citations: 12
Effect of yoga on ventilatory functions in normal healthy volunteers KJR Murthy, BK Sahay, S Madhavi, P Sitaramaraju, R Yogi, ... Lung India 1 (5), 189-92 , 1983 1983 Citations: 12
Value added products generation from sugarcane bagasse and its impact on economizing biorefinery and sustainability of sugarcane industry R Korasapati, SB Basthipati, K Rosangzuala, PR Reddy, R Gajjala, ... Sugarcane-Its Products and Sustainability , 2023 2023 Citations: 10
Bioprocess Optimization of L-Lysine Production by Using RSM and Artificial Neural Networks from Corynebacterium glutamicum ATCC13032 V Bhushanam, R Malothu Chemical Product and Process Modeling 15 (4), 20190054 , 2020 2020 Citations: 10
Analysis of radial vibrations of poroelastic circular cylindrical shells immersed in an acoustic medium M Ramesh, SA Shah, MVR Murthy International Journal of Engineering, Science and Technology 6 (5), 26-35 , 2014 2014 Citations: 9
Method development and stability indicating RP-HPLC method for the estimation of montelukast and fexofenadine for bulk and pharmaceutical dosage form MD Rameezuddin, PM Vasanth, T Ramesh, M Ramesh Int J Chem Tech Res 5, 2821-9 , 2013 2013 Citations: 9
Simultaneous estimation and forced degradation studies of amlodipine besylate and indapamide in tablet dosage form by RP-HPLC method G Rashmita, PM Vasanth, T Ramesh, M Ramesh Derpharma chemical 5 (6), 347-352 , 2013 2013 Citations: 8
RP-HPLC method development and validation for simultaneous estimation of Metformin and Sitagliptin in tablet dosage forms R Malothu, G Rani Sirisha, PM Vasanth, T Ramesh International Journal of Pharmaceutical Research & Analysis 3 (1), 8-12 , 2013 2013 Citations: 8
