Rakesh Kumar Gupta

RESEARCH, TEACHING, or OTHER INTERESTS

Molecular Biology, Food Science, Applied Microbiology and Biotechnology, Microbiology

Scopus Publications

Incidence of and risk factors for small vulnerable newborns in north India: a secondary analysis of a prospective pregnancy cohort
Ramachandran Thiruvengadam, Ayushi, Deepika Rathna Murugesan, Bapu Koundinya Desiraju, Sumit Misra, Dharmendra Sharma, Suresh Somi Subbaian, Umesh Mehta, Alka Singh, Sunita Sharma,et al.
Elsevier BV



Compliance to the Tobacco-Free Educational Institution(ToFEI) guidelines at Madhyamik Vidhyalayas of Pimpri-Chinchwad, Pune, Maharashtra-A cross sectional study
Sahana Hegde Shetiya, S.L. Jadhav, Supriya Kheur, Shivam Kapoor, and Rakesh Gupta
Elsevier BV



Development and external validation of Indian population-specific Garbhini-GA2 model for estimating gestational age in second and third trimesters
Veerendra P. Gadekar, Nikhita Damaraju, Ashley Xavier, Shambo Basu Thakur, Ramya Vijayram, Bapu Koundinya Desiraju, Sumit Misra, Shinjini Bhatnagar, Nitya Wadhwa, Uma Chandra Mouli Natchu,et al.
Elsevier BV



Integration of Medical Colleges under National Tobacco Control Program - A Public Health Priority
Rakesh Gupta and Sonu Goel
Medknow



Enforcement of COTPA in India- current status, challenges and solutions
Rakesh Gupta, Garima Bhatt, Ranjit Singh, Puneet Chahar, Sonu Goel, and Rana J Singh
Elsevier BV



The Transplant Dilemma: Uniting Rare Entities – Diabetic Nephropathy, Type 3 Diabetes Mellitus, and Acromegaly Secondary to Pituitary Macroadenoma
Arjun Agarwal, Dhananjai Agrawal, and Rakesh Gupta
Medknow
Diabetic nephropathy is a well-recognized complication of both Type 1 and Type 2 diabetes mellitus. This case report presents a unique and rare scenario of diabetes mellitus secondary to acromegaly, arising from a concealed, and long-standing pituitary macroadenoma. The report explores the challenges and dilemmas encountered in considering renal transplantation as a viable treatment option for this complex interplay of medical conditions.


Microbial weathering of rocks in natural habitat: Genetic basis and omics-based exploration
Mansi Podia, Prerna Yadav, Sunila Hooda, Prerna Diwan, and Rakesh K. Gupta
Wiley



Predicting Depth of Anesthesia using EEG Signals and Deep Convolution Network
Neeraj Kumar Sharma, Sakeena Shahid, Subodh Kumar, Sanjeev Sharma, Rakesh Kumar Gupta, and Naveen Kumar
ACM
Depth of Anesthesia is the degree of reversible unconsciousness achieved through anesthetic agents. An appropriate anesthetic depth must be maintained during surgical procedures to prevent perioperative complications. Electroencephalogram (EEG) signals allow us to capture the extent of brain activity in various stages of anesthesia. EEG signals thereby help anesthesiologists make informed decisions regarding the administration of anesthesia based on the extent of brain activity displayed by a patient. Bi-spectral Index (BIS) monitors use features extracted from EEG signal to display the depth of anesthesia as a value between 0 and 100. However, the algorithm behind BIS monitors’ functioning is unknown and these are costly machines. Under these circumstances, patient care or monitoring becomes entirely dependent on anesthesiologists’ experience. In this paper, we propose a model using deep Convolutional Neural Network (CNN) to predict the depth of anesthesia into three classes (Anesthesia Light, Anesthesia OK, Anesthesia Deep). The input of this network is a spectrogram obtained using Short-Time Fourier Transform (STFT) applied to EEG signals. This choice of using an STFT-derived spectrogram was made based on CNN’s superior performance over raw EEG waveforms-based CNN. The trained model achieved an accuracy of 90.8%. Experimental results reveal that the proposed scheme utilizing spectrograms achieves either superior or similar performance as compared to other works for accuracy and sensitivity even though it uses relatively fewer parameters. Further, beyond traditional evaluation metrics, this study employs F1-score and precision as additional criteria for assessing the proposed scheme, offering a comprehensive view of its efficacy.


Evaluation of a Hospitalized Pediatric COVID-19 Cohort from Indian National Clinical Registry of COVID-19
Alka Turuk, Gunjan Kumar, Aparna Mukherjee, Muralidharan Jayashree, Sweety M. Patel, Pankaj Bhardwaj, Thrilok Chander Bingi, Tridip Dutta Baruah, Lokesh Kumar Sharma, Geetha R. Menon,et al.
Springer Science and Business Media LLC



Circulatory Maternal Endothelin 1 and Matrix Metalloproteinase-9 Gene Expression in PREECLAMPSIA: A Study in Western Uttar Pradesh, India
Ravoori Saideswar Rao, Preeti Sharma, Mamta padhy, Ritu Sharma, Rakesh Gupta, Jayashree Bhatacharjee, and Manisha Singh
Springer Science and Business Media LLC



Evaluating Alterations of the Oral Microbiome and Its Link to Oral Cancer among Betel Quid Chewers: Prospecting Reversal through Probiotic Intervention
Prerna Diwan, Mohit Nirwan, Mayank Bahuguna, Shashi Prabha Kumari, James Wahlang, and Rakesh Kumar Gupta
MDPI AG
Areca nut and slaked lime, with or without tobacco wrapped in Piper betle leaf, prepared as betel quid, is extensively consumed as a masticatory product in many countries across the world. Betel Quid can promote the malignant transformation of oral lesions as well as trigger benign cellular and molecular changes. In the oral cavity, it causes changes at the compositional level in oral microbiota called dysbiosis. This dysbiosis may play an important role in Oral Cancer in betel quid chewers. The abnormal presence and increase of bacteria Fusobacterium nucleatum, Capnocytophaga gingivalis, Prevotella melaninogenica, Peptostreptococcus sp., Porphyromonas gingivalis, and Streptococcus mitis in saliva and/or other oral sites of the cancer patients has attracted frequent attention for its association with oral cancer development. In the present review, the authors have analysed the literature reports to revisit the oncogenic potential of betel quid and oral microbiome alterations, evaluating the potential of oral microbiota both as a driver and biomarker of oral cancer. The authors have also shared a perspective that the restoration of local microbiota can become a potentially therapeutic or prophylactic strategy for the delay or reversal of lip and oral cavity cancers, especially in high-risk population groups.


Genetic variants associated with spontaneous preterm birth in women from India: a prospective cohort study
Esha Bhattacharjee, Ramachandran Thiruvengadam, Ayushi, Chitrarpita Das, Nitya Wadhwa, Uma Chandra Mouli Natchu, Pallavi Kshetrapal, Shinjini Bhatnagar, Partha Pratim Majumder, Arindam Maitra,et al.
Elsevier BV



Prioritizing tobacco control & its cessation under sustainable development goals with a focus on India
Rakesh Gupta, Garima Bhatt, Sonu Goel, and RanaJugdeep Singh
Scientific Scholar
Sustainable development goals (SDGs) were meant to put each and everywhere 'at par'. The tobacco epidemic globally is one major deterrent to their achievement. While it gets addressed under SDG 3 through the Framework Convention on Tobacco Control (FCTC) - the World Health Organization (WHO) global treaty (the target 3.a of SDG 3), the progress made globally and by India is slow. As a result, many countries may fall short of achieving the target of reducing tobacco usage (taking 2016 as base year) by 30 per cent by the year 2030. India with its high burden of tobacco use and abysmally low quitting along with soaring economic costs of tobacco related diseases and deaths can do better with the engagement of multisectoral stakeholders to strengthen tobacco control under SDGs. Moreover, there is a need to emphasize that the goal of O - Offer to Quit of WHO MPOWER can be achieved through increasing 'onus' on policy makers, and strategists, and opportunities for masses, tobacco users, healthcare professionals (HCPs) and enforcers to have tobacco cessation delivered optimally. By doing so, the United Nations can significantly facilitate a reduction in tobacco use and the resultant economic costs. Furthermore, it will assist the WHO to fulfil the targets set for 2030 under SDG 3.a by the FCTC member countries. In addition, it will fulfil the vision and mission defined in the Chandigarh declaration of the 5th National Conference on Tobacco or Health for India to be tobacco free by 2030.


Factors associated with unexplained sudden deaths among adults aged 18-45 years in India – A multicentric matched case–control study
ManojVasant Murhekar, Manickam Ponnaiah, Tarun Bhatnagar, RizwanSuliankachi Abdulkader, Rajalakshmi Elumalai, Janani Surya, Kathiresan Jeyashree, MuthusamySanthosh Kumar, Ranjithkumar Govindaraju, JeromieWesley Vivian Thangaraj,et al.
Medknow
Background & objectives: In view of anecdotal reports of sudden unexplained deaths in India's apparently healthy young adults, linking to coronavirus disease 2019 (COVID-19) infection or vaccination, we determined the factors associated with such deaths in individuals aged 18-45 years through a multicentric matched case–control study. Methods: This study was conducted through participation of 47 tertiary care hospitals across India. Cases were apparently healthy individuals aged 18-45 years without any known co-morbidity, who suddenly (<24 h of hospitalization or seen apparently healthy 24 h before death) died of unexplained causes during 1st October 2021-31st March 2023. Four controls were included per case matched for age, gender and neighborhood. We interviewed/perused records to collect data on COVID-19 vaccination/infection and post-COVID-19 conditions, family history of sudden death, smoking, recreational drug use, alcohol frequency and binge drinking and vigorous-intensity physical activity two days before death/interviews. We developed regression models considering COVID-19 vaccination ≤42 days before outcome, any vaccine received anytime and vaccine doses to compute an adjusted matched odds ratio (aOR) with 95 per cent confidence interval (CI). Results: Seven hundred twenty nine cases and 2916 controls were included in the analysis. Receipt of at least one dose of COVID-19 vaccine lowered the odds [aOR (95% CI)] for unexplained sudden death [0.58 (0.37, 0.92)], whereas past COVID-19 hospitalization [3.8 (1.36, 10.61)], family history of sudden death [2.53 (1.52, 4.21)], binge drinking 48 h before death/interview [5.29 (2.57, 10.89)], use of recreational drug/substance [2.92 (1.1, 7.71)] and performing vigorous-intensity physical activity 48 h before death/interview [3.7 (1.36, 10.05)] were positively associated. Two doses lowered the odds of unexplained sudden death [0.51 (0.28, 0.91)], whereas single dose did not. Interpretation & conclusions: COVID-19 vaccination did not increase the risk of unexplained sudden death among young adults in India. Past COVID-19 hospitalization, family history of sudden death and certain lifestyle behaviors increased the likelihood of unexplained sudden death.


SCOPE: Surveillance of COVID-19 in pregnancy- results of a multicentric ambispective case-control study on clinical presentation and maternal outcomes in India between April to November 2020
Sunesh Kumar, Neerja Bhatla, K. Aparna Sharma, Ramesh Agarwal, Ankit Verma, Vanamail Perumal, Poonam Shiv Kumar, B. S. Garg, Shivaprasad S. Goudar, Yeshita V. Pujar,et al.
Public Library of Science (PLoS)
Objective To determine the clinical manifestations, risk factors, treatment modalities and maternal outcomes in pregnant women with lab-confirmed COVID-19 and compare it with COVID-19 negative pregnant women in same age group. Design Multicentric case-control study. Data sources Ambispective primary data collection through paper-based forms from 20 tertiary care centres across India between April and November 2020. Study population All pregnant women reporting to the centres with a lab-confirmed COVID-19 positive result matched with controls. Data quality Dedicated research officers extracted hospital records, using modified WHO Case Record Forms (CRF) and verified for completeness and accuracy. Statistical analysis Data converted to excel files and statistical analyses done using STATA 16 (StataCorp, TX, USA). Odds ratios (ORs) with 95% confidence intervals (CI) estimated using unconditional logistic regression. Results A total of 76,264 women delivered across 20 centres during the study period. Data of 3723 COVID positive pregnant women and 3744 age-matched controls was analyzed. Of the positive cases 56·9% were asymptomatic. Antenatal complications like preeclampsia and abruptio placentae were seen more among the cases. Induction and caesarean delivery rates were also higher among Covid positive women. Pre-existing maternal co-morbidities increased need for supportive care. There were 34 maternal deaths out of the 3723(0.9%) positive mothers, while covid negative deaths reported from all the centres were 449 of 72,541 (0·6%). Conclusion Covid-19 infection predisposed to adverse maternal outcomes in a large cohort of Covid positive pregnant women as compared to the negative controls.


Cross-sectional study: knowledge assessment of youth regarding the global public health threat of antibiotic resistance
Lalit Mohan, Shaubhik Anand, Muskan Mittal, Keshav Goyal, Akanksha, Aman Dixit, Rakesh Kumar Gupta, Rita Jain, and Prerna Diwan
Springer Science and Business Media LLC



The Applications of Biosensors and Biochips for Prognosis and Diagnosis of Diseases
Ravi Kumar Chaudhary, Vivek Gupta, Shivani Kalhan, Rakesh Gupta, Siddhartha, Md Kausar Neyaz, and Jyotsna Sharma
Springer Nature Singapore



Compliance of sections 4-7 of cigarette and other tobacco products act (COTPA) 2003 in India - A systematic review and meta-analysis
SahanaHegde Shetiya, Kunal Deshmukh, and Rakesh Gupta
Medknow
In view of the vast variability of the compliance of Sections 4-7 of COTPA in India, a need for a collective evaluation appeared appropriate and timely. The aim of this review was to specifically analyze the country wide compliance of Section 4,5,6a and 6b,7 of COTPA along with the displays of signage on No Smoking (NoSmok) and Tobacco Free Institution (TFI). Studies published between 2003-December 2020 reporting compliance/non compliance (C/NC) were reviewed. Eligible study designs were observational studies and brief reports. To obtain the effect measure, Med Calc version 20 was used. 60 full text articles were assessed for eligibility and included in the qualitative and quantitative synthesis. The compliance for the Sections 4,5, 6b and 7 was 71.97% (95%CI:61.87-81.05, I2 =99.70%), 58.95% (95%CI:44.90-72.28, I2=99.53%), 51.08% (95% CI:39.30-62.81, I2=99.46%), 57.60% (95%CI:37.58-76.40, I2=99.48%) respectively whereas for the NoSmok and TFI board display it was 42.30% (95%CI:32.53-52.39, I2=99.75%) and 26.81% (95%CI:9.94-48.25, I2=99.51) respectively. Compliance of the Section 6a, sale of tobacco products to and by the minors was 66.39% (CI:49.50-81.36, I2=99.69%) and 94.11% (95%CI:78.54-99.99, I2=99.76%) respectively. This study observed a significant variability in compliance of Sections 4-7 of COTPA. It points to a critical need for a robust, self – sustained and effective enforcement mechanism countrywide to eliminate potential for any NC. Amendments to the COTPA appear critical for a policy reform and an optimal implementation.


Identifying oral microbiome alterations in adult betel quid chewing population of Delhi, India
Mayank Bahuguna, Sunila Hooda, Lalit Mohan, Rakesh Kumar Gupta, and Prerna Diwan
Public Library of Science (PLoS)
The study targets to establish a factorial association of oral microbiome alterations (oral dysbiosis) with betel quid chewing habits through a comparison of the oral microbiome of Betel quid chewers and non-chewing individuals. Oral microbiome analysis of 22 adult individuals in the Delhi region of India through the 16S sequencing approach was carried out to observe the differences in taxonomic abundance and diversity. A significant difference in diversity and richness among Betel Quid Chewers (BQC) and Betel Quid Non-Chewers (BQNC) groups was observed. There were significant differences in alpha diversity among the BQC in comparison to BQNC. However, in the age group of 21–30 years old young BQC and BQNC there was no significant difference in alpha diversity. Similar result was obtained while comparing BQC and Smoker-alcoholic BQC. BQ smoker-chewers expressed significant variance in comparison to BQC, based on cluster pattern analysis. The OTU-based Venn Diagram Analysis revealed an altered microbiota, for BQ chewing group with 0–10 years exposure in comparison to those with 10 years and above. The change in the microbial niche in early chewers may be due to abrupt chemical component exposure affecting the oral cavity, and thereafter establishing a unique microenvironment in the long-term BQC. Linear discriminant analysis revealed, 55 significant features among BQC and Alcoholic-Smoker BQC; and 20 significant features among BQC and Smoker BQC respectively. The study shows the abundance of novel bacterial genera in the BQC oral cavity in addition to the commonly found ones. Since the oral microbiome plays a significant role in maintaining local homeostasis, investigating the link between its imbalance in such conditions that are known to have an association with oral diseases including cancers may lead to the identification of specific microbiome-based signatures for its early diagnosis.


Strategic initiatives to improve tobacco cessation delivery in India
Rakesh Gupta, MiraB Aghi, Arpit Gupta, Garima Bhatt, and Sonu Goel
Medknow
Background: Globally, India is recognized for providing comprehensive coverage of tobacco cessation through the infrastructure and resources over the last two decades. Nevertheless, its current tobacco burden is worrying due to an increase in ~2 million initiators and 5.87% tobacco related deaths annually. Objective: It was to identify and describe challenges and barriers in tobacco cessation delivery that exist at various levels of health care as well as at the level of tobacco users, their care givers and communities in which they live. Method: Besides authors' first-hand collective experience in the tobacco control for over 80 years and ~35 years in tobacco cessation and reviewed references, the stakeholders communications during various events along with telephonic or in-person with some of them were assimilated to comprehend an overall understanding of the issue. Results: The challenges and barriers are primarily due to low priority assigned by the relevant functionaries, the inadequacy of resources, poor engagement of health-and insurance-sectors and healthcare workers, a low intent to quit by its users, suboptimal and discontinuous enforcement of the Cigarette and Other Tobacco Products Act of 2003 (COTPA), and indifference of the non-users. Conclusion: The countrywide strategic initiatives required “as a package” should include political and bureaucratic commitment, mass communication on benefits of quitting, licensed current users quitting through a timeline, use of systems approach in tobacco cessation delivery, implementation, and enforcement of vendor licensing and the proposed amendments in COTPA. Their perceived benefits will become a win-win situation for all stakeholders engaged in tobacco cessation delivery.


Role of Rituximab in Patients with Resistant Nephrotic Syndrome




Deceased Donor Renal Transplantation: 6 Year Experience from a Tertiary Care Center in North India




Combatting the imbalance of sex ratio at birth: medium-term impact of India's National Programme of Beti Bachao Beti Padhao in the Haryana State of India
Rakesh Gupta, Girdhari Lal Singhal, Aarti Goyal, Gurjinder Kaur, Neha Purohit, and Shankar Prinja
Oxford University Press (OUP)
Abstract The Government of India initiated the Beti Bachao Beti Padhao (B3P) programme in 2015 as a flagship initiative to reduce gender imbalance in sex ratio at birth (SRB) and to ensure social protection of girls. The present study was conducted to evaluate the medium-term impact of B3P implementation in Haryana state, from 2015 to 2019, on SRB. Monthly data on SRB were collected for the entire state of Haryana through a civil registration system. Segmented time series regression analysis was used to estimate the variations in SRB after the B3P programme with the help of Winter’s additive interrupted time series model. The SRB in Haryana increased from 876 girls per 1000 boys in 2015 to 923 in 2019. The results of the model demonstrated that before the inception of intervention (pre-slope), there was a significant monthly change in SRB of 0.217 (95% confidence interval: 0.144–0.290). Following the B3P programme, SRB was found to increase by 0.835 per month, which implied that an increase of 0.618 (confidence interval: 0.338, 0.898) every month in SRB can be attributed to the B3P programme. This indicated that SRB for the state of Haryana increased at the rate of 7.42 units per year as a result of the B3P programme. B3P has led to a significant improvement in SRB in Haryana state. The continuity of efforts in the same direction with a sustained focus on behaviour change will further help achieve the goal of gender parity in births and child survival.


Correlation Between Ambulatory Blood Pressure Monitoring and Office Blood Pressure Measurement in Patients with Hypertension: A Community Study
C.Venkata S. Ram, Sameer Dani, Abraham Oomman, Rakesh Gupta, K.O. Ramachari, G.Praveen Kumar, Jayanthi Swaminathan, Sathyanarayana Kondati, and Ishita Sengupta
Elsevier BV



Comparison of first trimester dating methods for gestational age estimation and their implication on preterm birth classification in a North Indian cohort
Ramya Vijayram, , Nikhita Damaraju, Ashley Xavier, Bapu Koundinya Desiraju, Ramachandran Thiruvengadam, Sumit Misra, Shilpa Chopra, Ashok Khurana, Nitya Wadhwa,et al.
Springer Science and Business Media LLC
AbstractBackgroundDifferent formulae have been developed globally to estimate gestational age (GA) by ultrasonography in the first trimester of pregnancy. In this study, we develop an Indian population-specific dating formula and compare its performance with published formulae. Finally, we evaluate the implications of the choice of dating method on preterm birth (PTB) rate. This study’s data was from GARBH-Ini, an ongoing pregnancy cohort of North Indian women to study PTB.MethodsComparisons between ultrasonography-Hadlock and last menstrual period (LMP) based dating methods were made by studying the distribution of their differences by Bland-Altman analysis. Using data-driven approaches, we removed data outliers more efficiently than by applying clinical parameters. We applied advanced machine learning algorithms to identify relevant features for GA estimation and developed an Indian population-specific formula (Garbhini-GA1) for the first trimester. PTB rates of Garbhini-GA1 and other formulae were compared by estimating sensitivity and accuracy.ResultsPerformance of Garbhini-GA1 formula, a non-linear function of crown-rump length (CRL), was equivalent to published formulae for estimation of first trimester GA (LoA, − 0.46,0.96 weeks). We found that CRL was the most crucial parameter in estimating GA and no other clinical or socioeconomic covariates contributed to GA estimation. The estimated PTB rate across all the formulae including LMP ranged 11.27–16.50% with Garbhini-GA1 estimating the least rate with highest sensitivity and accuracy. While the LMP-based method overestimated GA by 3 days compared to USG-Hadlock formula; at an individual level, these methods had less than 50% agreement in the classification of PTB.ConclusionsAn accurate estimation of GA is crucial for the management of PTB. Garbhini-GA1, the first such formula developed in an Indian setting, estimates PTB rates with higher accuracy, especially when compared to commonly used Hadlock formula. Our results reinforce the need to develop population-specific gestational age formulae.

RECENT SCHOLAR PUBLICATIONS

Microhabitat influences on phage-bacteria dynamics in an abandoned mine for ecorestoration
S Karmakar, P Mukherjee, V Mishra, RK Gupta, R Kumar, P Srivastava, ...
Journal of Environmental Management 370, 122659 2024



Comparative assessment of microbiome and resistome of influent and effluent of sewage treatment plant and common effluent treatment plant located in Delhi, India using shotgun
PD P Yadav, SP Kumari, S Hooda, RK Gupta
Journal of Environmental Management 369, 122342 2024



An efficient method for isolating bacterial DNA from bioaerosols for metagenomics
SP Kumari, M Podia, S Hooda, P Diwan, RK Gupta
Aerosol Science and Technology, 1-13 2024



XAI-VSDoA: An Explainable AI-Based Scheme Using Vital Signs to Assess Depth of Anesthesia
RKG • Neeraj Kumar Sharma, Shakeena Shahid, Subodh Kumar, Sanjeev Sharma ...
IEEE Access 12, 119185-119206 2024



Insights into Kinases of ESKAPE Pathogens for Therapeutic Interventions
RKGVG Deepansh Mody, Priyanka Joshi, Monika Antil
Cardiovascular & Hematological Agents in Medicinal Chemistry 22 (03) 2024



Evaluating Alterations of the Oral Microbiome and Its Link to Oral Cancer among Betel Quid Chewers: Prospecting Reversal through Probiotic Intervention
P Diwan, M Nirwan, M Bahuguna, SP Kumari, J Wahlang, RK Gupta
Pathogens 12 (8), 996 2023



Identifying oral microbiome alterations in adult betel quid chewing population of Delhi, India
M Bahuguna, S Hooda, L Mohan, RK Gupta, P Diwan
Plos one 18 (1), e0278221 2023



Repurposing Novel Antagonists for Targeting p7 Viroporin of HCV Using In Silico Approach
SGVG Varsha Dwivedi, Rakesh Kumar Gupta, Amita Gupta, Vijay K Chaudhary
Letters in Drug Design & Discovery 19 (11), 969-981 2022



Cross-sectional Study on Prevalence of Betel Nut Chewing among the Youth of Meghalaya, North East Region of India: Development of Multifaceted Prevention Strategy
S Snigdha, T Bajwa, S Anand, L Mohan, K Goyal, M Mittal, KR Gupta, ...
Asian Pac. J. Health Sci 8 (3), 185-190 2021



TlyA protein of Mycobacterium leprae: a probable bio-marker of active infection
H Deval, K Katoch, DS Chauhan, AK Tyagi, RK Gupta, R Kamal, A Kumar, ...
Leprosy Review 87 (4), 501-515 2016



Microbiological and Physico-Chemical Quality of Groundwater at a Resettlement Colony, Madanpur Khadar in Delhi, India
GRK • Kaur J., Kaur S., Dashora V., Chaudhary Y., Nijhawan P., Saini S ...
DU Journal of Undergraduate Research and Innovation 1 (03), 26-38 2015



Endonuclease IV Is the major apurinic/apyrimidinic endonuclease in Mycobacterium tuberculosis and is important for protection against oxidative damage
RV Puri, N Singh, RK Gupta, AK Tyagi
PLoS One 8 (8), e71535 2013



Ferritin structure from Mycobacterium tuberculosis: comparative study with homologues identifies extended C-terminus involved in ferroxidase activity
G Khare, V Gupta, P Nangpal, RK Gupta, NK Sauter, AK Tyagi
PloS one 6 (4), e18570 2011



Structural ordering of disordered ligand-binding loops of biotin protein ligase into active conformations as a consequence of dehydration
V Gupta, RK Gupta, G Khare, DM Salunke, A Surolia, AK Tyagi
PLoS One 5 (2), e9222 2010



Dissecting the Role of Critical Residues and Substrate Preference of a Fatty Acyl-CoA Synthetase (FadD13) of Mycobacterium tuberculosis
G Khare, V Gupta, RK Gupta, R Gupta, R Bhat, AK Tyagi
PLoS One 4 (12), e8387 2009



Crystal Structure of Bfr A from Mycobacterium tuberculosis: Incorporation of Selenomethionine Results in Cleavage and Demetallation of Haem
V Gupta, RK Gupta, G Khare, DM Salunke, AK Tyagi
PloS one 4 (11), e8028 2009



Crystallization and preliminary X-ray diffraction analysis of biotin acetyl-CoA carboxylase ligase (BirA) from Mycobacterium tuberculosis
V Gupta, RK Gupta, G Khare, A Surolia, DM Salunke, AK Tyagi
Acta Crystallographica Section F: Structural Biology and Crystallization 2008



Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosis
V Gupta, RK Gupta, G Khare, DM Salunke, AK Tyagi
Acta Crystallographica Section F: Structural Biology and Crystallization 2008



Lux expression in eukaryotic cells
RK Gupta, SS Patterson, GS Sayler, SA Ripp
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States) 2007



Lux expression in eukaryotic cells
RK Gupta, SS Patterson, GS Sayler, SA Ripp
US Patent 7,300,792 2007

MOST CITED SCHOLAR PUBLICATIONS

Use of Saccharomyces cerevisiae BLYES Expressing Bacterial Bioluminescence for Rapid, Sensitive Detection of Estrogenic Compounds
J Sanseverino, RK Gupta, AC Layton, SS Patterson, SA Ripp, L Saidak, ...
Applied and environmental microbiology 71 (8), 4455-4460 2005
Citations: 195



Detection of heavy metal ions using protein-functionalized microcantilever sensors
S Cherian, RK Gupta, BC Mullin, T Thundat
Biosensors and Bioelectronics 19 (5), 411-416 2003
Citations: 124



Expression of the Photorhabdus luminescens lux genes (luxA, B, C, D, and E) in Saccharomyces cerevisiae
RK Gupta, SS Patterson, S Ripp, ML Simpson, GS Sayler
FEMS yeast research 4 (3), 305-313 2003
Citations: 108



Codon optimization of bacterial luciferase (lux) for expression in mammalian cells
SS Patterson, HM Dionisi, RK Gupta, GS Sayler
Journal of industrial microbiology and biotechnology 32 (3), 115-123 2005
Citations: 94



Ferritin structure from Mycobacterium tuberculosis: comparative study with homologues identifies extended C-terminus involved in ferroxidase activity
G Khare, V Gupta, P Nangpal, RK Gupta, NK Sauter, AK Tyagi
PloS one 6 (4), e18570 2011
Citations: 74



Crystal Structure of Bfr A from Mycobacterium tuberculosis: Incorporation of Selenomethionine Results in Cleavage and Demetallation of Haem
V Gupta, RK Gupta, G Khare, DM Salunke, AK Tyagi
PloS one 4 (11), e8028 2009
Citations: 49



Metallohistins: A new class of plant metal-binding proteins
RK Gupta, SV Dobritsa, CA Stiles, ME Essington, Z Liu, CH Chen, ...
Journal of protein chemistry 21, 529-536 2002
Citations: 41



Dissecting the Role of Critical Residues and Substrate Preference of a Fatty Acyl-CoA Synthetase (FadD13) of Mycobacterium tuberculosis
G Khare, V Gupta, RK Gupta, R Gupta, R Bhat, AK Tyagi
PLoS One 4 (12), e8387 2009
Citations: 35



Structural ordering of disordered ligand-binding loops of biotin protein ligase into active conformations as a consequence of dehydration
V Gupta, RK Gupta, G Khare, DM Salunke, A Surolia, AK Tyagi
PLoS One 5 (2), e9222 2010
Citations: 31



Endonuclease IV Is the major apurinic/apyrimidinic endonuclease in Mycobacterium tuberculosis and is important for protection against oxidative damage
RV Puri, N Singh, RK Gupta, AK Tyagi
PLoS One 8 (8), e71535 2013
Citations: 30



Incorporation of nisin in stirred yogurt. I. Effect on lactic and non-lactic organisms during storage.
RK Gupta, DN Prasad
1988
Citations: 16



Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosis
V Gupta, RK Gupta, G Khare, DM Salunke, AK Tyagi
Acta Crystallographica Section F: Structural Biology and Crystallization 2008
Citations: 14



Antibiotic activity of nisin in food preservation-a review.
RK Gupta, DN Prasad
1989
Citations: 14



Modified luciferase nucleic acids and methods of use
S Patterson, R Gupta, G Sayler, H Dionisi
US Patent App. 10/697,419 2004
Citations: 12



Genetic evidence for plasmid-encoded lactococcin production inLactococcus lactis subsp.lactis 484
RK Gupta, VK Batish
Current Microbiology 24, 231-238 1992
Citations: 11



Lux expression in eukaryotic cells
RK Gupta, SS Patterson, GS Sayler, SA Ripp
US Patent 7,300,792 2007
Citations: 10



Evaluating Alterations of the Oral Microbiome and Its Link to Oral Cancer among Betel Quid Chewers: Prospecting Reversal through Probiotic Intervention
P Diwan, M Nirwan, M Bahuguna, SP Kumari, J Wahlang, RK Gupta
Pathogens 12 (8), 996 2023
Citations: 7



Identifying oral microbiome alterations in adult betel quid chewing population of Delhi, India
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Plos one 18 (1), e0278221 2023
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Listeria monocytogenes in dairy products―an overview
DN Prasad, RK Gupta
MAN Microbiologie, aliments, nutrition 8 (4), 383-405 1990
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Screening of lactic streptococci for antibacterial activity, plasmid profiles and biochemical performance
RK Gupta, VK Batish
MAN Microbiologie, aliments, nutrition 8 (1), 45-52 1990
Citations: 7