Mallesham Bulle
@ttu.edu
Research Scientist, IGCAST, Texas Tech University
RESEARCH, TEACHING, or OTHER INTERESTS
Plant Science, General Biochemistry, Genetics and Molecular Biology, Molecular Biology, Horticulture
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Mallesham Bulle, Ajay Kumar Venkatapuram, Sadanandam Abbagani, and P.B. Kirti
Elsevier BV
Mallesham Bulle, Vijay Sheri, Mahender Aileni, and Baohong Zhang
MDPI AG
The world population’s growing demand for food is expected to increase dramatically by 2050. The agronomic productivity for food is severely affected due to biotic and abiotic constraints. At a global level, insect pests alone account for ~20% loss in crop yield every year. Deployment of noxious chemical pesticides to control insect pests always has a threatening effect on human health and environmental sustainability. Consequently, this necessitates for the establishment of innovative, environmentally friendly, cost-effective, and alternative means to mitigate insect pest management strategies. According to a recent study, using chloroplasts engineered with double-strand RNA (dsRNA) is novel successful combinatorial strategy deployed to effectively control the most vexing pest, the western flower thrips (WFT: Frankliniella occidentalis). Such biotechnological avenues allowed us to recapitulate the recent progress of research methods, such as RNAi, CRISPR/Cas, mini chromosomes, and RNA-binding proteins with plastid engineering for a plausible approach to effectively mitigate agronomic insect pests. We further discussed the significance of the maternal inheritance of the chloroplast, which is the major advantage of chloroplast genome engineering.
Mahender Aileni, Mallesham Bulle, Ramesh Naik Malavath, Satyamraj Thurpu, Kiranmayi Bandaram, Bhargavi Balkampeta, Meghana Marri, Vijaya Simha Reddy Singasani, and E. N. Murthy
Springer Science and Business Media LLC
Pooja Singh, Ranjan Kumar Sahoo, Mallesham Bulle, and Kapuganti Jagadis Gupta
Springer US
Mallesham Bulle, Reddy Kishorekumar, Pradeep K. Pathak, Aakanksha Wany, and Kapuganti Jagadis Gupta
Springer New York
Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany, and Kapuganti Jagadis Gupta
Springer New York
Reddy Kishorekumar, Mallesham Bulle, Aakanksha Wany, and Kapuganti Jagadis Gupta
Springer New York
Veeresh Lokesh, Girigowda Manjunatha, Namratha S. Hegde, Mallesham Bulle, Bijesh Puthusseri, Kapuganti Jagadis Gupta, and Bhagyalakshmi Neelwarne
MDPI AG
Nitric oxide (NO) is known to antagonize ethylene by various mechanisms; one of such mechanisms is reducing ethylene levels by competitive action on S-adenosyl-L-methionine (SAM)—a common precursor for both ethylene and polyamines (PAs) biosynthesis. In order to investigate whether this mechanism of SAM pool diversion by NO occur towards PAs biosynthesis in banana, we studied the effect of NO on alterations in the levels of PAs, which in turn modulate ethylene levels during ripening. In response to NO donor sodium nitroprusside (SNP) treatment, all three major PAs viz. putrescine, spermidine and spermine were induced in control as well as ethylene pre-treated banana fruits. However, the gene expression studies in two popular banana varieties of diverse genomes, Nanjanagudu rasabale (NR; AAB genome) and Cavendish (CAV; AAA genome) revealed the downregulation of SAM decarboxylase, an intermediate gene involved in ethylene and PA pathway after the fifth day of NO donor SNP treatment, suggesting that ethylene and PA pathways do not compete for SAM. Interestingly, arginine decarboxylase belonging to arginine-mediated route of PA biosynthesis was upregulated several folds in response to the SNP treatment. These observations revealed that NO induces PAs via l-arginine-mediated route and not via diversion of SAM pool.
Abhaypratap Vishwakarma, Aakanksha Wany, Sonika Pandey, Mallesham Bulle, Aprajita Kumari, Reddy Kishorekumar, Abir U Igamberdiev, Luis A J Mur, and Kapuganti Jagadis Gupta
Oxford University Press (OUP)
AbstractNitric oxide (NO) is now established as an important signalling molecule in plants where it influences growth, development, and responses to stress. Despite extensive research, the most appropriate methods to measure and localize these signalling radicals are debated and still need investigation. Many confounding factors such as the presence of other reactive intermediates, scavenging enzymes, and compartmentation influence how accurately each can be measured. Further, these signalling radicals have short half-lives ranging from seconds to minutes based on the cellular redox condition. Hence, it is necessary to use sensitive and specific methods in order to understand the contribution of each signalling molecule to various biological processes. In this review, we summarize the current knowledge on NO measurement in plant samples, via various methods. We also discuss advantages, limitations, and wider applications of each method.
Aprajita Kumari, Pradeep Kumar Pathak, Mallesham Bulle, Abir U Igamberdiev, and Kapuganti Jagadis Gupta
Oxford University Press (OUP)
AbstractPlant mitochondria possess two different pathways for electron transport from ubiquinol: the cytochrome pathway and the alternative oxidase (AOX) pathway. The AOX pathway plays an important role in stress tolerance and is induced by various metabolites and signals. Previously, several lines of evidence indicated that the AOX pathway prevents overproduction of superoxide and other reactive oxygen species. More recent evidence suggests that AOX also plays a role in regulation of nitric oxide (NO) production and signalling. The AOX pathway is induced under low phosphate, hypoxia, pathogen infections, and elicitor treatments. The induction of AOX under aerobic conditions in response to various stresses can reduce electron transfer through complexes III and IV and thus prevents the leakage of electrons to nitrite and the subsequent accumulation of NO. Excess NO under various stresses can inhibit complex IV; thus, the AOX pathway minimizes nitrite-dependent NO synthesis that would arise from enhanced electron leakage in the cytochrome pathway. By preventing NO generation, AOX can reduce peroxynitrite formation and tyrosine nitration. In contrast to its function under normoxia, AOX has a specific role under hypoxia, where AOX can facilitate nitrite-dependent NO production. This reaction drives the phytoglobin–NO cycle to increase energy efficiency under hypoxia.
Rajesh Yarra, Mallesham Bulle, Ramesh Mushke, and E. Narasimha Murthy
Elsevier BV
Ramesh Mushke, Rajesh Yarra, and Mallesham Bulle
Springer Science and Business Media LLC
Mallesham Bulle, Rajesh Yarra, and Sadanandam Abbagani
Springer Science and Business Media LLC
Mallesham Bulle, Deepa Rathakatla, Raghuvardhan Lakkam, Venugopal Rao Kokkirala, Mahender Aileni, Zhang Peng, and Sadanandam Abbagani
Springer Science and Business Media LLC
Radhika Tippani, Rajesh Yarra, Mallesham Bulle, Mahendar Porika, Sadanandam Abbagani, and Christopher Thammidala
Springer Science and Business Media LLC
Rajesh Yarra, Si-Jie He, Sadanandam Abbagani, Biao Ma, Mallesham Bulle, and Wan-Ke Zhang
Springer Science and Business Media LLC
Mallesham Bulle, Srinivas Kota, Deepa Rathakatla, Mahender Aileni, Venugopal Rao Kokkirala, Kranthi Kumar Gadidasu, and Sadanandam Abbagani
Informa UK Limited
An efficient in vitro leaf regeneration protocol has been established in Woodfordia fruticosa L. (Lathyraceae), a threatened woody shrub. The leaf segments derived from in vitro-grown plants were cultured on Murashige and Skoog's medium supplemented with Thidiazuron (2.27, 4.54, 6.81, and 9.08 μM) and 6-Benzyladenine (4.4, 8.90, 13.30, and 17.70 μM) alone or in combination with Indole-3-acetic acid (1.14 and 2.28 μM). Maximum number of shoots (15.60 ± 1.15) with highest shoot length (2.90 ± 0.24) were regenerated directly from the leaf explants with a combination of TDZ (4.54 μM) and IAA (2.28 μM), whereas the intervening callus phase was observed in the media supplemented with TDZ or BA alone. The in vitro-regenerated shoots were rooted (100%) on half-strength MS salts fortified with 4.90 μM IBA. The regenerated plantlets hardened in the greenhouse showed an 85% survival rate. ISSR PCR of in vitro-regenerated plantlets reveals their genetic fidelity with the mother plant.