Dr. Sarmad Moin
@gyanvihar.org
Associate Professor, School of Applied Sciences
Suresh Gyan Vihar University
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Plant Science, Molecular Biology, Biochemistry, Genetics and Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Gunjan Sharma, Zoya Zaidi, Mohammad Irfan Ali, and Sarmad Moin
Informatics Publishing Limited
The objective of this study was the assessment of antioxidant and cytotoxic activity from Tanacetum dolicophyllum. Soxhlet extraction has been used for preparing plant extracts for which five solvents have been used, i.e., Petroleum ether, benzene, ethyl acetate, ethanol, and water. Proteins, tannins, phlobatannins, amino acids, carbohydrates, alkaloids, steroids, flavonoids, glycosides, cardiac glycosides, and phenols were detected in the sample extracts. In the phytochemical screening of plant extracts, ethyl acetate and ethanolic extract show the best results which further leads to the investigation of antioxidant activity. Ferric-Reducing Antioxidant Power (FRAP), 2,2-diphenyl-2-picrylhydrazyl (DPPH), Reducing Power assay, and Phosphomolybdenum assays were performed to estimate antioxidant activity. In the RPA and TAC, the absorbance of sample extracts increases as there is an increase in concentration. In DPPH and FRAP assay, ethyl acetate showed good results. DPPH assay for ethyl acetate extract with 172.73(µg/ml) and ethanolic extract with 171.07(µg/ml) of IC50 values. The ethyl acetate extract had the highest antioxidant activity. The MTT assay was used to investigate the anticancer activity. The MTT assay discovered that the ethyl acetate extract had the highest anticancer activity against HeLa cells with an IC50 75 µg/mL as compared to a normal cell line J774A. Several compounds present in the extract of ethyl acetate acted as anticancer and contributed to cytotoxic activity.
Nalini . Tomer, Mohammad Irfan Ali, and Sarmad . Moin
Dr. Yashwant Research Labs Pvt. Ltd.
Digera muricata (L.) mart (Amaranthaceae) is a promising medicinal plant used as a prophylactic, antioxidant, anthelmintic, antimicrobial, allelopathic, and antidiabetic. The present study assesses the quantification of primary and secondary metabolites along with the antimicrobial potential of D. muricata. Qualitative and quantitative phytochemical analysis was undertaken to evaluate secondary metabolites extracted from leaf extracts of D. muricata by using standard methods. The antibacterial and antifungal potential was studied by using the disc diffusion method. A (gas chromatography-mass spectrometry) (GC–MS) study was carried out to recognize the bioactive molecules in the active fraction. D. muricata contained carbohydrates, proteins, steroids, amino acids, cardiac glycosides, coumarin, polyphenol, alkaloids, saponin, tannin, and flavonoids. Results also indicate that ethyl acetate extracts of D. muricata showed significant antimicrobial potential against Streptococcus agalactiae (ATCC13813), Klebsiella pneumoniae (MTCC432), Escherichia coli (MTCC730), Streptococcus pyogenes (MTCC1924), Macrophomina phaseolina, and Candida albicans (MTCC7315). These findings showed that the ethyl acetate extract of D. muricata confined bioactive molecules of therapeutic rank with significant antibacterial and antifungal activity.
Tarun Kumar Upadhyay, Mohammed Irfan Ali, Fahad Khan, Harsh Goel, Manas Mathur, Keshav Goyal, Sarmad Moin, Pratibha Pandey, Pranay Tanwar, Amit Baran Sharangi,et al.
Bentham Science Publishers Ltd.
: Nanotechnology has been extensively exploited for its enormous therapeutic and diagnostic potential in the management of multiple disorders. It employs nanomaterials as drug carriers with enhanced efficacy and limited side effects on normal tissues. A lot of nanomaterials have been studied and produced, imminently reforming the treatment and diagnostics of numerous malignancies, including cancer. The purpose of the present study is to explore the role of nanotechnology-based devices/therapies that have a vital function in the therapeutics and diagnostics of cancer with potential impact at three levels: early detection, tumor imaging, and drug delivery methods. Concentrating on cancer, promising nanotechnology-based approaches have been planned to satisfy the need for targeted specificity of traditional agents of chemotherapeutics, in addition to early recognition of malignant and precancerous lesions. Prostate cancer is the fifth most well-- known cancer worldwide and the second most usually detected cancer in men. Therefore, there is a crucial need to improve therapeutic prospects for the diagnosis and treatment of prostate cancer via the exploitation of the potential of nanomaterials for cell-targeted specificity and improved primary diagnosis of precancerous tumors. The present review, therefore, focuses on summarizing all prospective applications of nanotechnology in the prognosis and diagnosis of prostate cancer, which would further help researchers to elucidate a more potent therapeutic approach for the better management of prostate cancer in the days ahead.
Zoya Zaidi, Niladri Maiti, Mohammad Irfan Ali, Gunjan Sharma, Sarmad Moin, Harihara Padhy, G. L. Balaji, and Venkatesa Prabhu Sundramurthy
Hindawi Limited
Carbon nanodots are a recently discovered sort of carbon nanoparticles, demonstrating excellent fluorescence and physical-chemical properties that make them appealing for diagnostics and chemotherapeutics, including biosensing, bioimaging, and nanocarriers for drug delivery ground-breaking therapeutic agents in photothermal and photodynamic therapy. This critical review strongly focuses on the varied sorts of processes involved in the synthesis of carbon nanodots alongside the benefits and shortcomings. Furthermore, the multiple applications of carbon nanodots are established and used to develop potential theranostic nanoarchitectures. This review paper analyses with a discussion focusing on the discovery, synthesis processes, and diverse biomedical applications.
Sarmad Moin and
Advanced Research Publications
Pyrethroids have been developed as a powerful insecticide that is widely used for space spray and residual indoor spraying, with impregnated nets included in the vector control programme. Insecticide resistance reduces the effectiveness of pesticides. Therefore, the vector susceptibility condition of the Anopheles stephensi is monitored in order to select the active ones. The current study is being conducted in a malaria-ridden area in various parts of the Alwar region of Rajasthan, India, against the malaria vector An. stephensi. The Susceptibility study was conducted by the WHO standardized method using the diagnostic doses of DDT, Alpha-cypermethrin and Deltamethrin. An. stephensi showed intermediate resistance to DDT from all over the study while susceptible to Alpha-cypermethrin and Deltamethrin.
Mohammad Irfan Ali, Jyotsna Dayma, and Sarmad Moin
IGI Global
Nano dosage forms the drug delivery method of herbal formulation used for improving the efficacy and overwhelming the problems related to plant medicines. Therefore, incorporation of the nano-sized carrier in conventional plant-derived medicine is essential to cure more chronic and lifestyle diseases. Several nano dosage forms like polymeric nanoparticles, liposomes, lipid nanoparticles, nano-emulsion, proliposomes, etc. has great advantages for herbal medicine including bioavailability, enhancement of solubility, improvement of pharmacological activity, protection from toxicity, stability enhancement, sustained delivery, improving the distribution of macrophages, protection from chemical, and physical degradation. The chapter focused on the advantages of nano herbal formulations over traditional herbal formulations.
Sen Amit, Ali Mohammad Irfan, Sharma Gaurav, Tomer Nalini, and Moin Sarmad
A and V Publications
Sarmad Moin and
Advanced Research Publications
Pyrethroids are powerful insecticides used in the vector control program with impregnated mesh, and residual indoor sprays. However, resistance to insecticide reduces the effectiveness. The present susceptibility study carried out against theAnopheles Stephensi to monitor the sensitivity conditions of An. Stephensi vector, which raises the need to understand the state of vector resistance in the Dungarpur region of Rajasthan, India in order to better report vector-based interventions. The sensitivity study was carried out by the WHO standard method using recommended diagnostic doses of DDT, alpha-cypermethrin, permethrin, and deltamethrin. An. Stephensi showed resistance to DDT from the entire study while sensitive to alpha-cypermethrin, permethrin, and deltamethrin. The study looks at the selection and circulation of the appropriate insecticide’s molecule for a vector control program as insecticide need constant monitoring to develop effective vector control strategies such as improving insecticide by applying integrated biological and ecological methods.
Wilda Onyancha, Mohammad Irfan Ali, Gaurav Sharma, and Sarmad Moin
Diva Enterprises Private Limited
Onyancha Wilda, Gaurav Sharma, Sarmad Moin, and Mohammad Irfan Ali
Diva Enterprises Private Limited
Microorganisms are becoming resistant to numerous drugs faster than the development of an alternative or new therapy. Bacteria that were earlier easily denatured or inactivated with available conventional antibiotics currently have become resistant threatening both human and animals’ life. These organisms have become conventional due to their modes of acquisition of drug-resistant and as a consequence of gene mutations and transfer of resistant genes through gene swapping. The existence of antibiotics in the environment has enhanced selective pressure where the drug-resistant strains continue multiplying. Both high and lower antibiotic doses contribute to bacterial resistance. In most cases, resistance to a certain drug from a specific class leads to resistance to all other drugs in the same class. The resistance is further increased by bacteria releasing enzymes that deactivate the drugs, the efflux pump, and modification of the cell wall or alteration of the drug target site. Major factors that drive to drug-resistant microbes are : the misuse of the drugs by the patients, improper drug description, and swapping of the resistant genes between the bacteria. It is, therefore, important to understand the various ways of bacterial resistances as well as the drivers involved to raise their spread. Appropriate prevention and control measures can be implemented to limit the spread of these drug resistance bacteria.
Amit Sen, Nalini Tomer, Mohammad Irfan Ali, and Sarmad Moin
Diva Enterprises Private Limited
The genus Chenopodium from the Chenopodiaceae family comprises at least 150 plant species. These are distributed over a large geographical area from wild to domesticated forms with high diversity. Some species such as C. album and C. quinoa are notable for their nutritional value and are commonly consumed with diet. They are also well adapted to adverse climatic conditions and are important in the conservation of the environment. The pharmacological evaluation of Chenopodium plant species also ranged from the treatment of external ailment (wound healing activity) to the cellular level (anticancer and immunomodulatory activities). It is concluded that the genus has lots of potential for further scientific studies, search of the new bioactive molecules, and the development of the new herbal drug.
Lekha Singh, Gaurav Sharma, Asha Sharma, Gyanendra Awasthi, Lokendra Kumar, Mohammad Irfan Ali, and Sarmad Moin
Journal of Pure and Applied Microbiology
A thermophilic esterase isolated from Rhodococcus sp. LKE-021. This enzyme was purified with purification fold 60 from the crude extracts of enzyme and recovery of enzyme obtained approximately 21%. The specific activity of the lKe-021 esteraseis 795.1 U/mg. SDS-PAGE analysis determined the molecular weight of LKE-21 esteraseis around 32,000Da/32KDa. The enzyme activity of lKe-021 esterase exhibited over a wide range of temperature i.e. 30° to 80°C and the enzyme remained stable when incubated on 60° for 2h. This indicates that the isolated lKe-021 esterase is thermostable. The isolated enzyme exhibits activity on various pH ranges from 2.0 to 12.0 and the highest activity observed on 11.0 pH.The LKE-021 esterase was active after proteinase K treatment and shows over 75 % specific activity i.e. 50 U/μg Proteinase K.
Lekha Singh, Gaurav Sharma, Gyanendra Awasthi, Lokendra Kumar, Mohammad Irfan Ali, and Sarmad Moin
Journal of Pure and Applied Microbiology
Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, catalase positive, indole negative, positive to glucose and xylose fermentation test, and can grow on the Nutrient Broth medium. Esterase production confirmed on the basis of spectrophotometric enzyme assay. Taxonomic characteristics Rhodococcus confirmed by 16s rRNA gene sequencing.
Sarmad Moin, Chitra B. Devi, Servin P. Wesley, Shibu B. Sahaya, and Zoya Zaidi
Informa UK Limited
Abstract Loeseneriella obtusifolia (Roxb.) A.C. Smith and Cassine glauca (Rottb.) Kuntze are highly valued for their economic and medicinal properties. The petroleum ether, ethyl acetate, ethanol, and water extracts from mature leaves of both plants were screened individually for their chemical constituents and antibacterial activity. Water extract of both plants possess maximum phytochemical constituents followed by ethanol and ethyl acetate. Ethanol extracts of L. obtusifolia and C. glauca was active against the tested pathogenic bacteria. The strongest minimum inhibitory concentration (MIC) was observed against Staphylococcus aureus and Corynebacterium sp. at 0.250 mg mL-1 of the L. obtusifolia and C. glauca ethanol leaf extract, respectively. The antibacterial potential of the plants was then compared with positive (tetracycline) and negative (dimethyl sulfoxide) controls.