Carlos Rad
@investigacion.ubu.es
Faculty of Sciences, Chemistry Dpt. Soil and Agricultural Sciences Area
University of Burgos
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Soil Science, Environmental Science
Scopus Publications
Scopus Publications
Sandra Curiel-Alegre, Dalia de la Fuente-Vivas, Aqib Hassan Ali Khan, Javier García-Tojal, Blanca Velasco-Arroyo, Carlos Rumbo, Gerhard Soja, Carlos Rad, and Rocío Barros
Elsevier BV
Blanca Velasco-Arroyo, Sandra Curiel-Alegre, Aqib Hassan Ali Khan, Carlos Rumbo, Daniel Pérez-Alonso, Carlos Rad, Herwig De Wilde, Alfredo Pérez-de-Mora, and Rocío Barros
Elsevier BV
Juliana Ferreira Lorentz, Maria Lúcia Calijuri, Carlos Rad, Paulo Roberto Cecon, Paula Peixoto Assemany, Jorge Miñon Martinez, and Rajaa Kholssi
Springer Science and Business Media LLC
Sandra Curiel-Alegre, Aqib Hassan Ali Khan, Carlos Rad, Blanca Velasco-Arroyo, Carlos Rumbo, Rafael Rivilla, David Durán, Miguel Redondo-Nieto, Eduard Borràs, Daniele Molognoni,et al.
Springer Science and Business Media LLC
AbstractThe biodegradation of total petroleum hydrocarbon (TPH) in soil is very challenging due to the complex recalcitrant nature of hydrocarbon, hydrophobicity, indigenous microbial adaptation and competition, and harsh environmental conditions. This work further confirmed that limited natural attenuation of petroleum hydrocarbons (TPHs) (15% removal) necessitates efficient bioremediation strategies. Hence, a scaling-up experiment for testing and optimizing the use of biopiles for bioremediation of TPH polluted soils was conducted with three 500-kg pilots of polluted soil, and respective treatments were implemented: including control soil (CT), bioaugmentation and vermicompost treatment (BAVC), and a combined application of BAVC along with bioelectrochemical snorkels (BESBAVC), all maintained at 40% field capacity. This study identified that at pilot scale level, a successful application of BAVC treatment can achieve 90.3% TPH removal after 90 days. BAVC’s effectiveness stemmed from synergistic mechanisms. Introduced microbial consortia were capable of TPH degradation, while vermicompost provided essential nutrients, enhanced aeration, and, potentially, acted as a biosorbent. Hence, it can be concluded that the combined application of BAVC significantly enhances TPH removal compared to natural attenuation. While the combined application of a bioelectrochemical snorkel (BES) with BAVC also showed a significant TPH removal, it did not differ statistically from the individual application of BAVC, under applied conditions. Further research is needed to optimize BES integration with BAVC for broader applicability. This study demonstrates BAVC as a scalable and mechanistically sound approach for TPH bioremediation in soil.
Ángel Arroyo, Carlos Cambra, Nuño Basurto, Carlos Rad, Milagros Navarro, and Álvaro Herrero
Springer Nature Switzerland
Sandra de la Parra, Verónica González, Patricia Solórzano Vives, Sandra Curiel-Alegre, Blanca Velasco-Arroyo, Carlos Rad, Rocío Barros, Juan Antonio Tamayo-Ramos, and Carlos Rumbo
Elsevier BV
Sandra Curiel-Alegre, Blanca Velasco-Arroyo, Carlos Rumbo, Aqib Hassan Ali Khan, Juan Antonio Tamayo-Ramos, Carlos Rad, José Luis R. Gallego, and Rocío Barros
Elsevier BV
Lucie Mota, Violeta Hevia, Carlos Rad, Joana Alves, António Silva, José A. González, Jorge Ortega‐Marcos, Oscar Aguado, Paloma Alcorlo, Francisco M. Azcárate,et al.
Wiley
Abstract Intensification of agricultural landscapes to fulfil increased global food demands has dramatically impacted biodiversity and ecosystem services. Several pollinator groups, which are vital for the maintenance of pollinator‐dependent crops, have been severely affected by this intensification process. Management tools, such as the implementation of agri‐environmental schemes, have been widely proposed to improve pollinator's communities and pollination services, although the effectiveness of wildflower strips in comparison to existing natural or semi‐natural habitats and the impact on yield has not been fully demonstrated. Here, we aimed to assess the effect of flower strips implementation near sunflower fields in two intensive agricultural regions and to quantify their impact on visitation rates and sunflower productivity. Data were obtained in two regions in Spain (Burgos and Cuenca) in sunflower fields with associated semi‐natural vegetation (SNVs), with implemented wildflower strips (WFSs) and without vegetation structures (NonVs). Visitation rates were monitored over 2 years by direct observations, and both sunflower seed production and weight were assessed in 52 fields per year. Our results revealed regional and inter‐annual variation in visitation rates, likely driven by structural differences in the landscapes studied. In Cuenca, characterized by more heterogeneous and floral resources‐richer landscapes, the effects of WFSs were significant in the second year of implementation, with higher visitation rates and productivity values in fields with implemented wildflower strips compared to those without. In contrast, in Burgos, no consistent effects among field treatments between years were observed. Synthesis and applications. The implementation of flower strips or maintenance of remnant semi‐natural habitats adjacent to sunflower fields showed context‐dependent effects on pollinator visitation rates and crop yield. In highly simplified agroecosystems, these interventions may be insufficient or may need longer times to produce significant effects. Yet, in regions where natural and semi‐natural patches were already present, the implementation of flower strips was a successful strategy to promote pollinators and sunflower productivity.
Alfonso Allen‐Perkins, Ainhoa Magrach, Matteo Dainese, Lucas A. Garibaldi, David Kleijn, Romina Rader, James R. Reilly, Rachael Winfree, Ola Lundin, Carley M. McGrady,et al.
Wiley
AbstractSeventy five percent of the world's food crops benefit from insect pollination. Hence, there has been increased interest in how global change drivers impact this critical ecosystem service. Because standardized data on crop pollination are rarely available, we are limited in our capacity to understand the variation in pollination benefits to crop yield, as well as to anticipate changes in this service, develop predictions, and inform management actions. Here, we present CropPol, a dynamic, open, and global database on crop pollination. It contains measurements recorded from 202 crop studies, covering 3,394 field observations, 2,552 yield measurements (i.e., berry mass, number of fruits, and fruit density [kg/ha], among others), and 47,752 insect records from 48 commercial crops distributed around the globe. CropPol comprises 32 of the 87 leading global crops and commodities that are pollinator dependent. Malus domestica is the most represented crop (32 studies), followed by Brassica napus (22 studies), Vaccinium corymbosum (13 studies), and Citrullus lanatus (12 studies). The most abundant pollinator guilds recorded are honey bees (34.22% counts), bumblebees (19.19%), flies other than Syrphidae and Bombyliidae (13.18%), other wild bees (13.13%), beetles (10.97%), Syrphidae (4.87%), and Bombyliidae (0.05%). Locations comprise 34 countries distributed among Europe (76 studies), North America (60), Latin America and the Caribbean (29), Asia (20), Oceania (10), and Africa (7). Sampling spans three decades and is concentrated on 2001–2005 (21 studies), 2006–2010 (40), 2011–2015 (88), and 2016–2020 (50). This is the most comprehensive open global data set on measurements of crop flower visitors, crop pollinators and pollination to date, and we encourage researchers to add more datasets to this database in the future. This data set is released for non‐commercial use only. Credits should be given to this paper (i.e., proper citation), and the products generated with this database should be shared under the same license terms (CC BY‐NC‐SA).
Mercedes Yartu, Carlos Cambra, Milagros Navarro, Carlos Rad, Ángel Arroyo, and Álvaro Herrero
Elsevier BV
Rajaa Kholssi, Evan A.N. Marks, Jorge Miñón, Olimpio Montero, Juliana F. Lorentz, Abderrahmane Debdoubi, and Carlos Rad
Informa UK Limited
ABSTRACT There are a substantial number of studies on the biofertilization effects of cyanobacteria in rice paddy fields, mainly attributed to biological fixation of N2, but not much attention has been given to their fertilizing capacity in aerobic soils. Few studies have used solid media (i.e. a soil) when testing the plant-growth-promoting effects of microalgae on plants, and particularly on wheat. The purpose of this study was to test the biofertilizing effect of a filamentous cyanobacterium, previously isolated from an agricultural soil, in order to evaluate the potential substitution of chemical fertilizers and to test its phyto-stimulating capacity. Seedlings of Triticum aestivum were grown in pots with a peat-vermiculite mixture (1:1 weight basis) in an experiment designed as a complete randomized block, consisting of four treatments and with four replicates each: a pure culture of Anabaena cylindrica concentrated by centrifugation to 2 g dry matter L−1 (treatment B); spent cyanobacteria growth medium filtered at 0.22 µm (treatment F); harvested cyanobacterial mat re-suspended in distilled water (treatment WB); and distilled water as a control (treatment W). Aboveground wheat plant mass was improved by 40% in both treatments with cyanobacterial biomass (B and WB), as compared to the control (W) and filtrate (F), demonstrating that the co-cultivation with living cyanobacterial biomass was key to plant improvement. Chlorophyll contents were also increased by nearly 50% and nitrogen by over 10% in the treatment WB, clearly indicating that nutrients in the filtrate were irrelevant to the beneficial effects on plant growth.
Rajaa Kholssi, Evan A.N. Marks, Jorge Miñón, Ana Pascual Maté, Gonzalo Sacristán, Olimpio Montero, Abderrahmane Debdoubi, and Carlos Rad
Elsevier BV
Olimpio Montero, Marta Velasco, Jorge Miñón, Evan A. N. Marks, Aurelio Sanz-Arranz, and Carlos Rad
MDPI AG
The membrane glycerolipids of four phototrophs that were isolated from an edaphic assemblage were determined by UPLC–MS after cultivation in a laboratory growth chamber. Identification was carried out by 18S and 16S rDNA sequencing. The algal species were Klebsormidium flaccidum (Charophyta), Oocystis sp. (Chlorophyta), and Haslea spicula (Bacillariophyta), and the cyanobacterium was Microcoleus vaginatus (Cyanobacteria). The glycerolipid profile of Oocystis sp. was dominated by monogalactosyldiacylglycerol (MGDG) species, with MGDG(18:3/16:4) accounting for 68.6%, whereas MGDG(18:3/16:3) was the most abundant glycerolipid in K. flaccidum (50.1%). A ratio of digalactosyldiacylglycerol (DGDG) species to MGDG species (DGDG/MGDG) was shown to be higher in K. flaccidum (0.26) than in Oocystis sp. (0.14). This ratio increased under high light (HL) as compared to low light (LL) in all the organisms, with its highest value being shown in cyanobacterium (0.38–0.58, LL−HL). High contents of eicosapentaenoic acid (EPA, C20:5) and hexadecenoic acid were observed in the glycerolipids of H. spicula. Similar Fourier transform infrared (FTIR) and Raman spectra were found for K. flaccidum and Oocystis sp. Specific bands at 1629.06 and 1582.78 cm−1 were shown by M. vaginatus in the Raman spectra. Conversely, specific bands in the FTIR spectrum were observed for H. spicula at 1143 and 1744 cm−1. The results of this study point out differences in the membrane lipid composition between species, which likely reflects their different morphology and evolutionary patterns.
Josu Narbarte-Hernandez, Eneko Iriarte, Ángel Carrancho-Alonso, Asier Olazabal-Uzkudun, Carlos Rad, Martin Arriolabengoa, Arantza Aranburu, and Juan Antonio Quirós-Castillo
Elsevier BV
Rajaa Kholssi, Priscila Vogelei Ramos, Evan A.N. Marks, Olimpio Montero, and Carlos Rad
Elsevier BV
Javier García-Tojal, Eneko Iriarte, Susana Palmero, María R. Pedrosa, Carlos Rad, Silvia Sanllorente, María Cruz Zuluaga, Mónica Cavia-Saiz, Dolores Rivero-Perez, and Pilar Muñiz
Elsevier BV
Evan Marks, Hanene Akrout, Vasiliki Kinigopoulou, Charalampos Doulgeris, Salah Jellali, Carlos Rad, Paula Sánchez Zulueta, Evangelos Tziritis, Leila El-Bassi, and Mejdi Jeguirim
Springer International Publishing
Mercedes Yartu, Carlos Cambra, Milagros Navarro, Carlos Rad, Ángel Arroyo, and Álvaro Herrero
Springer International Publishing
Gonzalo Sacristán-Pérez-Minayo, Domingo Javier López-Robles, Carlos Rad, and Luis Miranda-Barroso
Frontiers Media SA
Used mainly for sucrose production, sugar beet is one of the most important crops in Castilla y León (Spain). Several studies have demonstrated the benefits of microorganisms in different crop management programs, among which Plant Growth Promoting Rhizobacteria (PGPR). This research aims to assess the beneficial effects of two PGPRs strains (Pseudomonas fluorescens Pf0-1 and Pseudomonas chlororaphis CECT 462) on sugar beet (Beta vulgaris) production. Three treatments: a PGPRs co-inoculation assay of untreated seeds without any chemical treatment (TB), a conventional treatment with commercial seeds and fungicide application (TT); and a control with seeds without protective coating, bacterial inoculation and chemical treatment (ST). The efficacy of PGPRs inoculation on sugar beet production was determined measuring periodically the photosynthetic status of plants, and the final yield and quality of tubers. Aerial and root plant biomass, maximum beet perimeter, polarization, and sugar values of the sugar beet plants inoculated with PGPRs showed higher values and significant differences to sugar beet subjected to other treatments. We could see that PGPRs inoculation (TB treatment) produced significant differences in the quantum yield of PSII (ΦPSII). TB showed the highest value for ΦPSII and the NPQ (non-photochemical quenching), the lowest value, even though the PSII (maximum quantum yield of photosystem II) was very similar in all treatments. The two assayed PGPR strains triggered a significant increase in sugar beet production yield and quality. PGPRs inoculation techniques could be used in different crops and they could be applied as biofertilizers, improving the agricultural production.
Evan A.N. Marks, Vasiliki Kinigopoulou, Hanene Akrout, Ahmed Amine Azzaz, Charalampos Doulgeris, Salah Jellali, Carlos Rad, Paula Sánchez Zulueta, Evangelos Tziritis, Leila El-Bassi,et al.
MDPI AG
Olive mill wastes continue to be a management challenge due to the large volumes produced, particularly due to their toxicity and impacts on the environment. Thermal conversion through pyrolysis or hydrothermal carbonization techniques can detoxify wastes while conserving nutrient contents. In this work, we produced up-to-date data on olive mill waste flows in Spain, Tunisia, and Greece and characterized representative samples in the laboratory. Assays of thermal conversion of olive mill wastewaters and solid wastes were also performed to understand biochar yields and final properties, and the total quantities of nutrients contained were estimated. Of particular note were the quantities of potassium in Tunisian wastewaters, representing 0.6% of the total mass and an annual flow of approximately 5000 t, and in the Spanish solid wastes, an average of 1.7% of the total mass is potassium, representing an annual flow of approximately 23,000 t. Concerning phosphorus, Spanish solid wastes had the highest contents (0.1%), double that of other countries’ wastes. Annually, olive mill wastes from the three countries are estimated to contain approximately 35 × 103 tons of potassium and 2.6 × 103 tons of phosphorus. With this resource converted to biochar, each year more than 700 km2 of soils could be enriched in 0.2% carbon with biochar at an application rate of 7 t ha−1.
Josu Narbarte-Hernández, Eneko Iriarte, Carlos Rad, Carlos Tejerizo, Javier Fernández Eraso, and Juan Antonio Quirós-Castillo
Elsevier BV
Ahmed Amine Azzaz, Mejdi Jeguirim, Evan A. N. Marks, Carlos Rad, Salah Jellali, Mary-Lorène Goddard, and Camelia Matei Ghimbeu
Cellule MathDoc/CEDRAM
In this study, we assessed the transformation of raw olive pomace into carbon-rich material using olive mill wastewater (OMWW) as the liquid medium for the hydrothermal carbonization (HTC) process. The findings were compared accordingly with the use of distilled water (DW), which is the conventional practice. The use of OMWW as a liquid matrix enhanced the hydrochar yield, but volatile matter, fixed carbon content, and O/C and H/C ratios followed a decreasing trend. Furthermore, for an HTC temperature of 220 °C, the use of OMWW considerably increased the high heating value of the hydrochars from approximately 24.2 MJ/kg to 31.6 MJ/kg. According to the van Krevelen diagram of feedstock and derived hydrochars, dehydration was the predominant carbonization reaction for both liquid sources. Morphological characterization of both sets of hydrochars indicated the generation of specific carbon nuclei when using DW while OMWW led to the creation of hydrochar with a less ∗Corresponding author. ISSN (electronic) : 1878-1543 https://comptes-rendus.academie-sciences.fr/chimie/ 636 Ahmed Amine Azzaz et al. homogeneous surface. Structural analysis revealed the heterogeneous aspect of the hydrochar surface with an abundance of crystallized metal-based inorganic salts.
Evan A.N. Marks, Olimpio Montero, and Carlos Rad
Elsevier BV
Josu Narbarte-Hernández, Eneko Iriarte, Carlos Rad, Ángel Carrancho-Alonso, Penélope González-Sampériz, Leonor Peña-Chocarro, and Juan Antonio Quirós-Castillo
Elsevier BV
Rajaa Kholssi, Evan A. N. Marks, Jorge Miñón, Olimpio Montero, Abderrahmane Debdoubi, and Carlos Rad
Springer Science and Business Media LLC