Investigation of the Intensity of Thrombocytosis as a Potential Prognostic Indicator in Canine Malignant Neoplasms Letícia Gondim Souto, Fwu Shing Teng, Susana Eduardo Vieira, Regina Kiomi Takahira Veterinary Medicine International, 2026 Platelets contribute to cancer progression, and thrombocytosis is a recognized poor prognostic factor in human oncology; however, its significance in veterinary medicine remains underexplored. We retrospectively analyzed 166 dogs diagnosed with neoplasia and thrombocytosis (platelet count ≥ 430,000/μL) to assess the relationship between thrombocytosis intensity and survival. Survival analyses revealed that compared with dogs with mild thrombocytosis, those with moderate‐to‐severe thrombocytosis had significantly shorter survival times. With respect to specific diagnoses, dogs with mammary carcinoma and moderate‐to‐severe thrombocytosis had a significantly higher risk of death (HR = 3.4; p = 0.02). A similar trend was observed in patients with mast cell tumors, where the risk of death was 7.39 times higher, although this finding reached only borderline statistical significance ( p = 0.05). Further prospective studies with larger cohorts are warranted to validate thrombocytosis as a prognostic biomarker and to elucidate its role in tumor biology and clinical outcomes in veterinary oncology.
Assessment of Renal Function and Bilirubin Measurement in Urine as Prognostic Value in Immune-Mediated Hemolytic Anemia in Dogs IVANIO T. BORBA-JUNIOR, ROBERTA M. BASSO, TATIANA G. GORENSTEIN, NAYARA LIMONTA, BRUNA DOS SANTOS, et al. Anais Da Academia Brasileira De Ciencias, 2026 Immune-mediated hemolytic anemia (IMHA) is the leading cause of hemolytic anemia in dogs. Although renal injury has been reported in IMHA patients, laboratory findings defining the type and extent of renal damage remain inconsistent. The aim of this study was to evaluate key laboratory findings in the assessment of renal function in dogs with a suspected diagnosis of IMHA and to correlate these findings with disease prognosis. A prospective study was conducted on 21 dogs treated at a veterinary hospital with a suspected diagnosis of IMHA. Blood samples were collected for complete blood count and biochemical analyses, and urinalysis was performed. Significant alterations in renal function were observed in IMHA patients. The urinary protein-to-creatinine ratio (UPC) was elevated compared to controls (P < 0.0002) and correlated with the degree of anemia (R = -0.638) and the inflammatory status, assessed by white blood cell count (R = 0.550). In addition, urinary bilirubin levels were increased in IMHA patients (P < 0.0001), suggesting that bilirubin is an important prognostic marker of the disease. Renal function is impaired in IMHA patients, and urinary bilirubin levels serve as a valuable prognostic indicator.
Comparative Effects of Aminophylline, Caffeine, and Doxapram in Hypoxic Neonatal Dogs Born by Cesarean Section Júlia Cosenza Mendonça, Keylla Helena Nobre Pacífico Pereira, Gleice Mendes Xavier, Kárita da Mata Fuchs, Thaís Gomes Faustino, et al. Animals, 2025 Background: Perinatal hypoxia is a leading cause of early mortality in canine neonates and accounts for over 90% of early deaths in canine neonates. Respiratory depression may result from dystocia, prolonged labor, or anesthetic protocols used during cesarean delivery, requiring rapid pharmacological support. This study evaluated the effects of aminophylline, caffeine, and doxapram on clinical and biochemical viability parameters in hypoxic neonatal dogs. Methods: Forty-five puppies born via elective or emergency cesarean section were included, of which 35 exhibiting cardiorespiratory depression (heart rate ≤ 180 bpm, respiratory rate < 15 movements per minute, Apgar score < 7, and reflex score < 3) were randomly allocated into aminophylline group (AG; n = 12), caffeine group (CafG; n = 11), or doxapram group (DG; n = 12). Ten clinically healthy neonates constituted the physiological control group (CG). Apgar score, neonatal reflexes, lactate, blood glucose, oxygen saturation, blood gas parameters, and cardiac troponin I (cTnI) were evaluated immediately after birth (M1) and 10 min following treatment (M10). Results: No significant differences were observed in laboratory parameters among groups (p > 0.05). Although all evaluated drugs improved clinical status by increasing heart rate, respiratory rate, and reflex scores, caffeine produced the greatest enhancement in vitality. The Δ Apgar score showed a significant difference between CafG and DG (p = 0.0095), while no statistical differences were detected between AG and CafG (p = 0.08) or between AG and GD (p = 0.60). Conclusions: Aminophylline, caffeine, and doxapram supported postnatal resuscitation in hypoxic neonatal puppies; however, caffeine demonstrated superior improvement in Apgar recovery, suggesting its potential as a preferred pharmacological adjunct in neonatal canine resuscitation protocols.
Outbreak of Trypanosoma vivax in Dairy Cattle from São Paulo, Brazil: Haematological and Molecular Diagnostic Approaches Karoline Koether, Ana L. H. de Albuquerque, Luiza S. Zakia, Fernanda Panseri Rodrigues, José P. Oliveira-Filho, et al. Parasitologia, 2025 Trypanosoma vivax, a haemoprotozoan of African origin, has spread throughout Latin America, mainly affecting cattle through mechanical transmission by flies. This study reports an outbreak of T. vivax on a dairy farm in the midwestern region of São Paulo State, Brazil. Clinical signs included progressive weight loss, lethargy, hyporexia, reduced milk production, abortions, neurological signs, and deaths. The herd comprised 238 crossbred Holstein-Gir cattle (200 cows, 38 calves). Blood samples were collected from 104 randomly selected cows and all 38 calves to assess hematocrit (HCT) and total plasma protein (TPP), and detect trypomastigote forms using blood and buffy coat smears. PCR was performed on EDTA blood from 30 smear-negative cows. Trypanosoma sp. was identified in 51.9% (54/104) of the smear samples, with morphometric confirmation of T. vivax infection. About 66.6% (20/30) of the smear-negative cows tested positive by PCR, showing the higher sensitivity of molecular tests. Anemia was observed in 56.8% of infected cows, with significantly lower HCT and TPP values than in uninfected animals. The absence of quarantine for new animals, high fly density, and shared needles for oxytocin injections were likely contributing factors. This study underscores the crucial role of molecular diagnostics in accurately investigating outbreaks.
Vagal indigestion in mini-cattle1 Lucas V.O. Ferreira, Maria L.G. Lourenço, Regina K. Takahira, Juliana M. Alonso, José P. Oliveira-Filho, et al. Pesquisa Veterinaria Brasileira, 2023
Cerebrospinal fluid analysis in horses, cattle, and sheep diagnosed with rabies: A retrospective study of 62 cases Canadian Veterinary Journal, 2022
Evaluations hematological and biochemical by the use of sodium diclofenac, meloxicam and firocoxib in rats Brazilian Journal of Veterinary Research and Animal Science, 2010
Reference values of the urea and creatinine in wild boar (sus scrofa scrofa, linnaeus, 1758) raised in captivity Archives of Veterinary Science, 2010
Development of a dog model of hemodialysis Andre M.C Meneses, Jacqueline C.T Caramori, João R.A.C Brant, Raquel C Gonçalves, Nazaré F. de Souza, et al. Pesquisa Veterinaria Brasileira, 2010
First report of strongyloides sp. (Nematoda, Strongyloididae) in Leopardus tigrinus (Carnivora: Felidae) in the municipality of Botucatu, State of São Paulo, Brazil Revista Brasileira De Parasitologia Veterinaria, 2009
[Occurrence of gastrointestinal parasites in dogs and cats in the laboratory of routine of parasitic diseases FMVZ/Unesp-Botucatu, SP]. Revista Brasileira De Parasitologia Veterinaria Brazilian Journal of Veterinary Parasitology Orgao Oficial do Colegio Brasileiro De Parasitologia Veterinaria, 2008
Assessment of Renal Function and Bilirubin Measurement in Urine as Prognostic Value in Immune-Mediated Hemolytic Anemia in Dogs IT Borba-Junior, RM Basso, TG Gorenstein, N Limonta, BDOS SANTOS, ... Anais da Academia Brasileira de Ciências 98 (1), e20241141 , 2026 2026
Establishing the Plasma Proteomic Baseline of Crotalus durissus terrificus for Health Monitoring IT Makida, AA Resende, LG de Pontes, LA de Oliveira, L Melo, ... Analytical Biochemistry, 116051 , 2026 2026
Investigation of the Intensity of Thrombocytosis as a Potential Prognostic Indicator in Canine Malignant Neoplasms LG Souto, FS Teng, SE Vieira, RK Takahira Veterinary Medicine International 2026 (1), 1271185 , 2026 2026
Reference Plasma Proteome Map of Crotalus durissus terrificus. IT Makida, AA Resende, LG de Pontes, LA de Oliveira, L Melo, ... Anal Biochem, 116051-116051 , 2026 2026
Comparative Effects of Aminophylline, Caffeine, and Doxapram in Hypoxic Neonatal Dogs Born by Cesarean Section JC Mendonça, KHNP Pereira, GM Xavier, KM Fuchs, TG Faustino, ... Animals 15 (23), 3485 , 2025 2025
Feasibility and safety of intrathecal transplantation of allogeneic bone marrow mesenchymal stem cells in horses LV de Oliveira Ferreira, L Maia, DJ Barberini, RK Takahira, ... Veterinary Research Communications 49 (6), 333 , 2025 2025
Biochemical parameters of lambs under frequent treatment with antiparasitic association HJS Bello, JGG Lins, NMM da Silva, ACA de Albuquerque, ... Small Ruminant Research, 107662 , 2025 2025
Clinical, laboratory, and ultrasonographic insights into Platynosomum fastosum infection in domestic cats: diagnostic challenges and hepatobiliary implications LMN Sato, HS Carrenho, RK Takahira, PF Marcusso, A Melchert, ... The Veterinary Journal, 106442 , 2025 2025 Citations: 1
Outbreak of Trypanosoma vivax in Dairy Cattle from São Paulo, Brazil: Haematological and Molecular Diagnostic Approaches K Koether, ALH de Albuquerque, LS Zakia, FP Rodrigues, ... Parasitologia 5 (3), 42 , 2025 2025 Citations: 1
Laboratorial parameters of septic neonate dogs after adjuvant treatment with fresh frozen plasma KH Pereira, K Fuchs, G Xavier, JC Mendonca, D Camara, LEC Correia, ... REPRODUCTION IN DOMESTIC ANIMALS 60 , 2025 2025
Evaluation of biochemical alterations in canine neonatal sepsis K Fuchs, KH Pereira, G Xavier, JC Mendonca, D Camara, LEC Correia, ... REPRODUCTION IN DOMESTIC ANIMALS 60 , 2025 2025
Evaluation of the safety and feasibility of extracorporeal therapy: therapeutic plasma exchange in dogs-report of five cases SR Maia, RS de Moraes, SS Geraldes, MGP de Azevedo, AG Stefaniszen, ... BMC Veterinary Research 21 (1), 348 , 2025 2025
Traumatic reticulopericarditis in cattle: a retrospective case series LV de Oliveira Ferreira, GG Macedo, RK Takahira, NS Rocha, ... Large Animal Review 31 (2), 107-111 , 2025 2025 Citations: 1
Evaluation of intermittent hemodialysis therapy in the bypass mode in dogs with chronic kidney disease in uremic crisis MGP de Azevedo, SR Maia, RS de Moraes, SS Geraldes, HDM García, ... BMC Veterinary Research 21 (1), 286 , 2025 2025 Citations: 1
Chemical sensors and biosensors for point-of-care testing of pets: Opportunities for individualized diagnostics of companion animals WT Fonseca, T Parra Vello, GC Lelis, AV Ferreira Deleigo, RK Takahira, ... ACS sensors 10 (5), 3222-3238 , 2025 2025 Citations: 11
Suspected chlorfenapyr poisoning in a horse BP Simões, FM Cerri, RK Takahira, AS Borges, JP Oliveira-Filho, ... Journal of Equine Veterinary Science 146, 105380 , 2025 2025
Valores de hemogasometria venosa de urubus (Coragyps atratus) de vida livre RM Basso, FM Cerri, WS Ferreira, IT Borba Junior, LS Rolim, RK Takahira, ... Universidade Federal Rural do Semi-Árido (UFERSA) , 2025 2025
Periovulatory anticoagulant therapy enhances embryo recovery rates in superovulated mares LT Rodrigues, L Segabinazzi, M Frasson, C Dell’Aqua, MT Carmo, ... Theriogenology 231, 182-189 , 2025 2025
Serum concentrations of IL-1β, IL-6, and TNF-α in dogs with chronic kidney disease in uremic syndrome undergoing intermittent hemodialysis with and without bypass SR Maia, MG Picelli de Azevedo, SS Geraldes, RS de Moraes, ... Veterinary Quarterly 44 (1), 1-12 , 2024 2024 Citations: 2
A retrospective description of blood and urine alterations in 386 male cats with urethral obstruction in Botucatu, São Paulo, Brazil RS de Moraes, D Ribeiro, A Melchert, HDM García, D Regalin, ... Open veterinary journal 14 (11), 2901 , 2024 2024 Citations: 4
MOST CITED SCHOLAR PUBLICATIONS
Evaluation of adverse effects of long-term oral administration of carprofen, etodolac, flunixin meglumine, ketoprofen, and meloxicam in dogs SPL Luna, AC Basílio, PVM Steagall, LP Machado, FQ Moutinho, ... American journal of veterinary research 68 (3), 258-264 , 2007 2007 Citations: 312
The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area C Bulla, R Takahira, JP Araújo Jr, L Aparecidatrinca, R Lopes, ... Veterinary Research 35 (1), 141-146 , 2004 2004 Citations: 199
Citrus aurantium L. essential oil exhibits anxiolytic-like activity mediated by 5-HT 1A -receptors and reduces cholesterol after repeated oral treatment CARA Costa, TC Cury, BO Cassettari, RK Takahira, JC Flório, M Costa BMC complementary and alternative medicine 13 (1), 42 , 2013 2013 Citations: 170
Beta‐caryophyllene as an antioxidant, anti‐inflammatory and re‐epithelialization activities in a rat skin wound excision model LFS Gushiken, FP Beserra, MF Hussni, MT Gonzaga, VP Ribeiro, ... Oxidative medicine and cellular longevity 2022 (1), 9004014 , 2022 2022 Citations: 139
Cholesterol reduction and lack of genotoxic or toxic effects in mice after repeated 21-day oral intake of lemongrass (Cymbopogon citratus) essential oil CARA Costa, LT Bidinotto, RK Takahira, DMF Salvadori, LF Barbisan, ... Food and Chemical Toxicology 49 (9), 2268-2272 , 2011 2011 Citations: 128
From inflammation to cutaneous repair: Topical application of lupeol improves skin wound healing in rats by modulating the cytokine levels, NF-κB, Ki-67, growth factor … F Pereira Beserra, LF Sergio Gushiken, AJ Vieira, D Augusto Bérgamo, ... International journal of molecular sciences 21 (14), 4952 , 2020 2020 Citations: 125
Canine hepatozoonosis in Brazil: description of eight naturally occurring cases LFP Gondim, A Kohayagawa, NX Alencar, AW Biondo, RK Takahira, ... Veterinary Parasitology 74 (2-4), 319-323 , 1998 1998 Citations: 115
Constituents and antiulcer effect of Alchornea glandulosa: activation of cell proliferation in gastric mucosa during the healing process TR Calvo, ZP Lima, JS Silva, KVR Ballesteros, CH Pellizzon, ... Biological and Pharmaceutical Bulletin 30 (3), 451-459 , 2007 2007 Citations: 84
Effect of menthol in experimentally induced ulcers: Pathways of gastroprotection AL Rozza, CA Hiruma-Lima, RK Takahira, CR Padovani, CH Pellizzon Chemico-Biological Interactions 206 (2), 272-278 , 2013 2013 Citations: 81
The analgesic effect of crotoxin on neuropathic pain is mediated by central muscarinic receptors and 5-lipoxygenase-derived mediators F de Sousa Nogueira-Neto, RL Amorim, P Brigatte, G Picolo, ... Pharmacology Biochemistry and Behavior 91 (2), 252-260 , 2008 2008 Citations: 55
Amniocentesis and biochemical evaluation of amniotic fluid in ewes at 70, 100 and 145 days of pregnancy NC Prestes, MCL Chalhoub, MD Lopes, RK Takahira Small Ruminant Research 39 (3), 277-281 , 2001 2001 Citations: 50
Studies of gastric mucosa regeneration and safety promoted by Mouriri pusa treatment in acetic acid ulcer model PCP Vasconcelos, H Kushima, M Andreo, CA Hiruma-Lima, W Vilegas, ... Journal of Ethnopharmacology 115 (2), 293-301 , 2008 2008 Citations: 47
Pre-and post-operative evaluations of eight dogs following right nephrectomy due to Dioctophyma renale LR Mesquita, SC Rahal, LG Faria, RK Takahira, NS Rocha, MJ Mamprim, ... Veterinary Quarterly 34 (3), 167-171 , 2014 2014 Citations: 46
Ultrasonographic, computed tomographic, and operative findings in dogs infested with giant kidney worms ( Dioctophyme renale ) SC Rahal, MJ Mamprim, HS Oliveira, LR Mesquita, LG Faria, RK Takahira, ... Journal of the American Veterinary Medical Association 244 (5), 555-558 , 2014 2014 Citations: 37
Hemostatic profile and thromboembolic risk in healthy dogs treated with prednisone: a randomized controlled trial FG Romão, EF Campos, CRS Mattoso, RK Takahira BMC veterinary research 9 (1), 268 , 2013 2013 Citations: 36
Seasonal influence on hematologic values and hemoglobin electrophoresis in Brazilian Boa constrictor amarali CC Machado, LFN Silva, PRR Ramos, RK Takahira Journal of Zoo and Wildlife Medicine 37 (4), 487-491 , 2006 2006 Citations: 35
Routine screening examinations in attendance of cats with obstructive lower urinary tract disease AM Neri, LH de Araújo Machado, PTCG Okamoto, MG Filippi, ... Topics in companion animal medicine 31 (4), 140-145 , 2016 2016 Citations: 34
Effects of dipyrone, meloxicam, or the combination on hemostasis in conscious dogs FS Zanuzzo, FJ Teixeira‐Neto, CM Thomazini, RK Takahira, B Conner, ... Journal of Veterinary Emergency and Critical Care 25 (4), 512-520 , 2015 2015 Citations: 33
Valores hematológicos de capivaras (Hydrochoerus hydrochaeris) criadas em cativeiro no município de Botucatu, SP ME Arouca, LB Miranda, RS Lopes, RK Takahira, A Kohayagawa, ... Ciência Rural 30, 813-817 , 2000 2000 Citations: 33