Joao Manuel Cunha Rodrigues
@cqm.uma.pt
- Departamento de Química/Faculdade de Ciências Exactas e da Engenharia - Centro de Química da Madeira
Universidade da Madeira
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Jaison Jeevanandam and João Rodrigues
Elsevier BV
Jinxia Wang, Zhiqiang Wang, Guixiang Zhang, João Rodrigues, Helena Tomás, Xiangyang Shi, and Mingwu Shen
Royal Society of Chemistry (RSC)
Blood–brain barrier-crossing dendrimers for glioma imaging diagnostics, chemotherapy, gene therapy, or imaging-guided therapy have been concisely reviewed with perspectives briefly discussed.
Yue Gao, Zhijun Ouyang, Gaoming Li, Qiuyu Yu, Waicong Dai, João Rodrigues, Andrij Pich, Meera Moydeen Abdul Hameed, Mingwu Shen, and Xiangyang Shi
American Chemical Society (ACS)
Ye Tian, Zhiqiang Wang, Xu Xu, Yunqi Guo, Yanni Ma, Yanqi Lu, Mingwu Shen, Yang Geng, Helena Tomás, João Rodrigues,et al.
Informa UK Limited
Herein, we isolated five natural alkaloids, iso-corydine (iso-CORY), corydine (CORY), sanguinarine (SAN), chelerythrine (CHE) and magnoflorine (MAG), from traditional medicinal herb Dicranostigma leptopodum (Maxim.) Fedde (whole herb) and elucidated their structures. Then we synthesised G5. NHAc-PBA as targeting dendrimer platform to encapsulate the alkaloids into G5. NHAc-PBA-alkaloid complexes, which demonstrated alkaloid-dependent positive zeta potential and hydrodynamic particle size. G5. NHAc-PBA-alkaloid complexes demonstrated obvious breast cancer MCF-7 cell targeting effect. Among the G5. NHAc-PBA-alkaloid complexes, G5.NHAc-PBA-CHE (IC50=13.66 μM) demonstrated the highest MCF-7 cell inhibition capability and G5.NHAc-PBA-MAG (IC50=24.63 μM) had equivalent inhibitory effects on cell proliferation that comparable to the level of free MAG (IC50=23.74 μM), which made them the potential breast cancer targeting formulation for chemotherapeutic application. This work successfully demonstrated a pharmaceutical research model of 'natural bioactive product isolation-drug formulation preparation-breast cancer cell targeting inhibition'.
Jinkai Zhang, Yu Zhuang, Ruilong Sheng, Helena Tomás, João Rodrigues, Guangyin Yuan, Xudong Wang, and Kaili Lin
Royal Society of Chemistry (RSC)
Schematic summary of various smart stimuli-responsive strategies applied for titanium implant functionalization.
Jaison Jeevanandam, Mara Gonçalves, Rita Castro, Juan Gallo, Manuel Bañobre-López, and João Rodrigues
Elsevier BV
Guizhi Zhang, Mengsi Zhan, Changchang Zhang, Zhiqiang Wang, Huxiao Sun, Yuchen Tao, Qiusheng Shi, Meijuan He, Han Wang, João Rodrigues,et al.
Wiley
Developing a multifunctional nanoplatform to achieve efficient theranostics of tumors through multi-pronged strategies remains to be challenging. Here, the design of the intelligent redox-responsive generation 3 (G3) poly(amidoamine) dendrimer nanogels (NGs) loaded with gold nanoparticles (Au NPs) and chemotherapeutic drug toyocamycin (Au/Toy@G3 NGs) for ultrasound-enhanced cancer theranostics is showcased. The constructed hybrid NGs with a size of 193 nm possess good colloidal stability under physiological conditions, and can be dissociated to release Au NPs and Toy in the reductive glutathione-rich tumor microenvironment (TME). The released Toy can promote the apoptosis of cancer cells through endoplasmic reticulum stress amplification and cause immunogenic cell death to maturate dendritic cells. The loaded Au NPs can induce the conversion of tumor-associated macrophages from M2-type to antitumor M1-type to remodulate the immunosuppressive TME. Combined with antibody-mediated immune checkpoint blockade, effective chemoimmunotherapy of a pancreatic tumor mouse model can be realized, and the chemoimmunotherapy effect can be further ultrasound enhanced due to the sonoporation-improved tumor permeability of NGs. The developed Au/Toy@G3 NGs also enable Au-mediated computed tomography imaging of tumors. The constructed responsive dendrimeric NGs tackle tumors through a multi-pronged chemoimmunotherapy strategy targeting both cancer cells and immune cells, which hold a promising potential for clinical translations.
Ivo J. Martins, Helena Tomás, and João Rodrigues
Elsevier BV
Xichao Hu, Yingjun Ke, Huan Ye, Beitong Zhu, João Rodrigues, and Ruilong Sheng
Elsevier BV
Jaison Jeevanandam, Kei Xian Tan, João Rodrigues, and Michael K. Danquah
MDPI AG
Nanosized Janus and dendrimer particles have emerged as promising nanocarriers for the target-specific delivery and improved bioavailability of pharmaceuticals. Janus particles, with two distinct regions exhibiting different physical and chemical properties, provide a unique platform for the simultaneous delivery of multiple drugs or tissue-specific targeting. Conversely, dendrimers are branched, nanoscale polymers with well-defined surface functionalities that can be designed for improved drug targeting and release. Both Janus particles and dendrimers have demonstrated their potential to improve the solubility and stability of poorly water-soluble drugs, increase the intracellular uptake of drugs, and reduce their toxicity by controlling the release rate. The surface functionalities of these nanocarriers can be tailored to specific targets, such as overexpressed receptors on cancer cells, leading to enhanced drug efficacy The design of these nanocarriers can be optimized by tuning the size, shape, and surface functionalities, among other parameters. The incorporation of Janus and dendrimer particles into composite materials to create hybrid systems for enhancing drug delivery, leveraging the unique properties and functionalities of both materials, can offer promising outcomes. Nanosized Janus and dendrimer particles hold great promise for the delivery and improved bioavailability of pharmaceuticals. Further research is required to optimize these nanocarriers and bring them to the clinical setting to treat various diseases. This article discusses various nanosized Janus and dendrimer particles for target-specific delivery and bioavailability of pharmaceuticals. In addition, the development of Janus-dendrimer hybrid nanoparticles to address some limitations of standalone nanosized Janus and dendrimer particles is discussed.
Mengsi Zhan, Huxiao Sun, Joao Rodrigues, Dzmitry Shcharbin, Mingwu Shen, and Xiangyang Shi
Future Medicine Ltd
Cláudia Camacho, Dina Maciel, Helena Tomás, and João Rodrigues
MDPI AG
Cisplatin (cis-diamminedichloroplatinum(II)) is a potent chemotherapeutic agent commonly used to treat cancer. However, its use also leads to serious side effects, such as nephrotoxicity, ototoxicity, and cardiotoxicity, which limit the dose that can be safely administered to patients. To minimize these problems, dendrimers may be used as carriers for cisplatin through the coordination of their terminal functional groups to platinum. Here, cisplatin was conjugated to half-generation anionic PAMAM dendrimers in mono- and bidentate forms, and their biological effects were assessed in vitro. After preparation and characterization of the metallodendrimers, their cytotoxicity was evaluated against several cancer cell lines (A2780, A2780cisR, MCF-7, and CACO-2 cells) and a non-cancer cell line (BJ cells). The results showed that all the metallodendrimers were cytotoxic and that the cytotoxicity level depended on the cell line and the type of coordination mode (mono- or bidentate). Although, in this study, a correlation between dendrimer generation (number of carried metallic fragments) and cytotoxicity could not be completely established, the monodentate coordination form of cisplatin resulted in lower IC50 values, thus revealing a more accessible cisplatin release from the dendritic scaffold. Moreover, most of the metallodendrimers were more potent than the cisplatin, especially for the A2780 and A2780cisR cell lines, which showed higher selectivity than for non-cancer cells (BJ cells). The monodentate G0.5COO(Pt(NH3)2Cl)8 and G2.5COO(Pt(NH3)2Cl)32 metallodendrimers, as well as the bidentate G2.5COO(Pt(NH3)2)16 metallodendrimer, were even more active towards the cisplatin-resistant cell line (A2780cisR cells) than the correspondent cisplatin-sensitive one (A2780 cells). Finally, the effect of the metallodendrimers on the hemolysis of human erythrocytes was neglectable, and metallodendrimers’ interaction with calf thymus DNA seemed to be stronger than that of free cisplatin.
Jaison Jeevanandam, João Rodrigues, Sharadwata Pan, and Michael K. Danquah
Elsevier
Dina Maciel, Nádia Nunes, Francisco Santos, Yu Fan, Gaoming Li, Mingwu Shen, Helena Tomás, Xiangyang Shi, and João Rodrigues
Royal Society of Chemistry (RSC)
Pre-clinical results highlight the potential of the low-generation poly(alkylidenamine)-based dendrimers as ruthenium metallodrug nanocarriers.
Huxiao Sun, Mengsi Zhan, Serge Mignani, Dzmitry Shcharbin, Jean-Pierre Majoral, João Rodrigues, Xiangyang Shi, and Mingwu Shen
MDPI AG
Curcumin (Cur), a traditional Chinese medicine extracted from natural plant rhizomes, has become a candidate drug for the treatment of diseases due to its anti-inflammatory, anticancer, antioxidant, and antibacterial activities. However, the poor water solubility and low bioavailability of Cur limit its therapeutic effects for clinical applications. A variety of nanocarriers have been successfully developed to improve the water solubility, in vivo distribution, and pharmacokinetics of Cur, as well as to enhance the ability of Cur to polarize macrophages and relieve macrophage oxidative stress or anti-apoptosis, thus accelerating the therapeutic effects of Cur on inflammatory diseases. Herein, we review the design and development of diverse Cur nanoformulations in recent years and introduce the biomedical applications and potential therapeutic mechanisms of Cur nanoformulations in common inflammatory diseases, such as arthritis, neurodegenerative diseases, respiratory diseases, and ulcerative colitis, by regulating macrophage behaviors. Finally, the perspectives of the design and preparation of future nanocarriers aimed at efficiently exerting the biological activity of Cur are briefly discussed.
Jaison Jeevanandam, Saravanan Krishnan, Yiik Siang Hii, Sharadwata Pan, Yen San Chan, Caleb Acquah, Michael K. Danquah, and João Rodrigues
Springer Science and Business Media LLC
Beitong Zhu, Ruilong Sheng, Tianhong Chen, João Rodrigues, Qin-Hua Song, Xichao Hu, and Lintao Zeng
Elsevier BV
Jaison Jeevanandam, Sharadwata Pan, João Rodrigues, M. Abd Elkodous, and Michael K. Danquah
Royal Society of Chemistry (RSC)
This review is an overview of various biopolymer nanofibers and their distinct synthesis approaches. Further, the medical applications of biopolymer nanofibers, including drug delivery systems and biosensor fabrication, are also discussed.
Zeyu Fu, Yu Zhuang, Jinjie Cui, Ruilong Sheng, Helena Tomás, João Rodrigues, Bin Zhao, Xudong Wang, and Kaili Lin
Elsevier BV
Serge Mignani, Xiangyang Shi, João Rodrigues, Helena Tomás, and Jean-Pierre Majoral
Elsevier BV
Lirong Jiang, Huan Ye, Dini Ma, João Rodrigues, Ruilong Sheng, and Douyong Min
Royal Society of Chemistry (RSC)
Fish-based food products play important roles in our daily diet. The related food safety is vitally essential for human health, thus it is very necessary to screen the freshness of fish-based foods.
Jaison Jeevanandam, Siaw Fui Kiew, Stephen Boakye-Ansah, Sie Yon Lau, Ahmed Barhoum, Michael K. Danquah, and João Rodrigues
Royal Society of Chemistry (RSC)
Green synthesis approaches are gaining significance as promising routes for the sustainable preparation of nanoparticles, offering reduced toxicity towards living organisms and the environment.
Shewaye Lakew Mekuria, Zhijun Ouyang, Cong Song, João Rodrigues, Mingwu Shen, and Xiangyang Shi
American Chemical Society (ACS)
Recent advances in the field of nanotechnology bring an alternative approach to personalized medicine in cancer treatment. Nanogels (NGs) are among the nanosized superconstructs composed of amphiphilic or hydrophilic polymer networks. The design of different types of biodegradable polymer-based NGs in various biomedical applications has received extensive attention, due to their unique physicochemical properties such as highly porous structure, stimuli-responsiveness, and mimicking of some biological properties. In this review, we concisely surveyed the synthesis of dendrimer-based NGs synthesized via different methods including covalent conjugation, inverse nanoprecipitation, physical cross-linking, or self-assembly for various cancer nanomedicine applications, particularly for drug delivery, gene delivery, photothermal therapy, and combination therapy, as well as for biological imaging-guided chemotherapy. Additionally, we provide herein future perspective toward the new design of dendrimer-based NGs for different cancer nanomedicine uses.
Monika Müllerová, Dina Maciel, Nádia Nunes, Dominika Wrobel, Marcel Stofik, Lucie Červenková Št́astná, Alena Krupková, Petra Cuřínová, Kateřina Nováková, Matěj Božík,et al.
American Chemical Society (ACS)
The complexity of drug delivery mechanisms calls for the development of new transport system designs. Here, we report a robust synthetic procedure toward stable glycodendrimer (glyco-DDM) series bearing glucose, galactose, and oligo(ethylene glycol)-modified galactose peripheral units. In vitro cytotoxicity assays showed exceptional biocompatibility of the glyco-DDMs. To demonstrate applicability in drug delivery, the anticancer agent doxorubicin (DOX) was encapsulated in the glyco-DDM structure. The anticancer activity of the resulting glyco-DDM/DOX complexes was evaluated on the noncancerous (BJ) and cancerous (MCF-7 and A2780) cell lines, revealing their promising generation- and concentration-dependent effect. The glyco-DDM/DOX complexes show gradual and pH-dependent DOX release profiles. Fluorescence spectra elucidated the encapsulation process. Confocal fluorescence microscopy demonstrated preferential cancer cell internalization of the glyco-DDM/DOX complexes. The conclusions were supported by computer modeling. Overall, our results are consistent with the assumption that novel glyco-DDMs and their drug complexes are very promising in drug delivery and related applications.
Mara Gonçalves, Visvaldas Kairys, João Rodrigues, and Helena Tomás
American Chemical Society (ACS)