Mohamed Omar Taqi Aboubakr
@arc.sci.eg
Central Laboratory for Agricultural Climate
Agricultural Research Center
EDUCATION
2015 – 2019 Ph.D. of Agriculture Science. Thesis title: Transcription Factors mediated oxidative stress response in Bovine Follicular Cells and Preimplantation Embryos
Institute of Animal Science, Faculty of Agriculture, University of Bonn, Bonn, Germany
2007 – 2012 Master of Agriculture Science. Thesis title: Milk Productivity and Physiological parameters of Egyptian Buffalo under climatic conditions of Giza and Qena Governorates
Faculty of Agriculture, Cairo University, Giza, Egypt
2000 – 2004 Bachelor of Agriculture Science (Animal Production)
Faculty of Agriculture, Cairo University, Giza, Egypt
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Animal Science and Zoology, Biochemistry, Genetics and Molecular Biology, Aquatic Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Sally Ibrahim, Mohamed O. Taqi, A.S.A. Sosa, Al-Shimaa Al-H.H. El-Naby, Karima Gh.M. Mahmoud, Hassan R.H. Darwish, Amal R. Abd El Hameed, and M.F. Nawito
Elsevier BV
Sally Ibrahim, Mohamed Hedia, Mohamed O. Taqi, Mohamed K. Derbala, Karima Gh. M. Mahmoud, Youssef Ahmed, A. S. Sosa, Yasser H. A. Saber, M. H. Hasanain, M. F. Nawito,et al.
Springer Science and Business Media LLC
AbstractBackgroundExtracellular vesicles (EVs) are a promising biomarker and play a vital role in cell–cell communication. This study aimed (I) to identify and characterize EVs from low volume uterine lavage (LVL) and serum in mares with endometritis, compared to healthy controls and (II) to measure serum levels of interleukin 6 (IL-6), and prostaglandins (PGF2αand PGE2). Mares were divided into 30 sub-fertile (endometritis) and 20 fertile (controls). Serum and LVL was collected for EV isolation, and determination of serum levels of inflammatory mediators. Characterization and visualization of EVs were done by electron microscopy, dynamic light scattering and flow cytometry.ResultsSerial ultracentrifugation of LVL and use of a commercial kit for serum were strategies for EVs isolation. Mares with endometritis released higher amounts of larger size EVs. The EVs from mares with endometritis differentially expressed CD9 and CD63, compared to controls. Mares suffering from endometritis evoked higher levels of inflammatory mediators.ConclusionsThus, EVs could be used for a better understanding the regulatory mechanisms associated with developing endometritis in mares.
Mohamed Omar Taqi, Mohammed Saeed-Zidane, Samuel Gebremedhn, Dessie Salilew-Wondim, Ernst Tholen, Christiane Neuhoff, Michael Hoelker, Karl Schellander, and Dawit Tesfaye
Springer Science and Business Media LLC
AbstractTranscription factors (TFs) are known to be involved in regulating the expression of several classes of genes during folliculogenesis. However, the regulatory role of TFs during oxidative stress (OS) is not fully understood. The current study was aimed to investigate the regulation of the TFs in bovine granulosa cells (bGCs) during exposure to OS induced by H2O2in vitro. For this, bGCs derived from ovarian follicles were cultured in vitro till their confluency and then treated with H2O2for 40 min. Twenty-four hours later, cells were subjected to various phenotypic and gene expression analyses for genes related to TFs, endoplasmic reticulum stress, apoptosis, cell proliferation, and differentiation markers. The bGCs exhibited higher reactive oxygen species accumulation, DNA fragmentation, and endoplasmic reticulum stress accompanied by reduction of mitochondrial activity after exposure to OS. In addition, higher lipid accumulation and lower cell proliferation were noticed in H2O2-challenged cells. The mRNA level of TFs includingNRF2,E2F1,KLF6,KLF9,FOS,SREBF1,SREBF2, andNOTCH1was increased in H2O2-treated cells compared with non-treated controls. However, the expression level ofKLF4and its downstream gene,CCNB1, were downregulated in the H2O2-challenged group. Moreover, targeted inhibition ofNRF2using small interference RNA resulted in reduced expression ofKLF9,FOS,SREBF2, andNOTCH1genes, while the expression ofKLF4was upregulated. Taken together, bovine granulosa cells exposed to OS exhibited differential expression of various transcription factors, which are mediated by the NRF2 signaling pathway.
Sally Ibrahim, Mohamed Hedia, Mohamed O. Taqi, Mohamed K. Derbala, Karima Gh. M. Mahmoud, Youssef Ahmed, Sayed Ismail, and Mohamed El-Belely
MDPI AG
So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF2α and PGE2) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4–7 years), and old (8–14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF2α, and PGE2, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE2, and PGF2α were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE2, and PGF2α concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine.
Ahmed A. A. Abdel-Wareth, Mohammed Ali Al-Kahtani, Khalid Mushabab Alsyaad, Fatma Mohsen Shalaby, Islam M. Saadeldin, Fahdah Ayed Alshammari, Muhammad Mobashar, Mohamed H. A. Suleiman, Abdalla H. H. Ali, Mohamed O. Taqi,et al.
MDPI AG
The present study aimed to determine the effects of zinc oxide nanoparticles (ZnO-NPs), thyme oil (THO), or their combination on the nutrient digestibility coefficients, reproductive parameters, and some blood metabolites of male Californian rabbits. One hundred rabbits, 29-weeks of age (initial body weight 3.48 ± 0.08 kg) were randomly distributed into four groups, 25 rabbits each. Treatment groups were fed a control diet, a control diet supplemented with ZnO-NPs (100 mg/kg), THO (500 mg/kg), or combination of ZnO-NPs (100 mg/kg) and THO (500 mg/kg). The feeding trial lasted for 35 days. Results showed improvements in dry matter, crude protein, ether extract, and crude fiber in ZnO-NPs, THO, and their combination treated groups compared to those of control. Furthermore, semen volume, sperm motility, vitality, and morphology were significantly improved (p < 0.01) in ZnO-NPs and THO groups rather than the control. Both ZnO-NPs and THO, as either individual or combined treatments significantly improved the serum alanine amino-transferase (ALT), aspartate amino-transferase (AST), urea, and creatinine compared to the control. Moreover, serum concentrations of testosterone were significantly increased in rabbits supplemented with ZnO-NPs, THO, or their combination compared to those of control (p < 0.05). In conclusion, ZnO-NPs, THO, or their combination improved the digestibility of nutrients, liver/ kidney functions, semen characteristics, and testosterone concentration in male rabbits.
Mohamed O. Taqi, Mohammed Saeed‐Zidane, Samuel Gebremedhn, Dessie Salilew‐Wondim, Omar Khdrawy, Franca Rings, Christiane Neuhoff, Michael Hoelker, Karl Schellander, and Dawit Tesfaye
Wiley
AbstractSexually dimorphic differences in genome activity, which is orchestrated by transcription factors (TFs), could explain the differential response of male and female embryos to environmental stressors. To proof this hypothesis, the expression of cellular and extracellular TFs was investigated in male and female bovine embryos in vitro cultured either under low (5%) or high (20%) oxygen levels. The intracellular reactive oxygen species (ROS), total cell number, expression of nuclear factor (erythroid‐derived 2) factor 2 (NFE2L2), Krüppel‐like factor 4 (KLF4), notch receptor 1 (NOTCH1), E2F transcription factor 1 (E2F1), and SREBF2 along with extracellular vesicles (EVs) biogenesis genes were assessed at the blastocyst stage and their released EVs. Low blastocyst rate in both sexes due to oxidative stress (OS) was accompanied by increased ROS accumulation and reduced cell number in female embryos. The messenger RNA and protein levels of NFE2L2, as well as KLF4 expression, were higher in male embryos exposed to OS compared with female embryos. However, the expression of NOTCH1 and E2F1 was higher in female embryos cultured in high oxygen level. Male embryos exposed to OS released more EVs enriched with NFE2L2, superoxide dismutase 1, and NOTCH1 accompanied by elevated expression of EVs biogenesis genes. Accordingly, differential expression of TFs and their release into spent media could partially explain the sexual dimorphic response of bovine embryos to environmental stresses.
Omar Khadrawy, Samuel Gebremedhn, Dessie Salilew-Wondim, Mohamed Taqi, Christiane Neuhoff, Ernst Tholen, Michael Hoelker, Karl Schellander, and Dawit Tesfaye
MDPI AG
Nrf2 is a redox sensitive transcription factor regulating the expression of antioxidant genes as defense mechanism against various stressors. The aim of this study is to investigate the potential role of noncoding miRNAs as endogenous and quercetin as exogenous regulators of Nrf2 pathway in bovine granulosa cells. For this cultured granulosa cells were used for modulation of miRNAs (miR-28, 153 and miR-708) targeting the bovine Nrf2 and supplementation of quercentin to investigate the regulatory mechanisms of the Nrf2 antioxidant system. Moreover, cultured cells were treated with hydrogen peroxide to induce oxidative stress in those cells. Our results showed that, oxidative stress activated the expression of Nrf2 as a defense mechanism, while suppressing the expression of those miRNAs. Overexpression of those miRNAs resulted in downregulation of Nrf2 expression resulted in higher ROS accumulation, reduced mitochondrial activity and cellular proliferation. Quercetin supplementation showed its protective role against oxidative stress induced by H2O2 by inducing the expression of antioxidant enzymes. In conclusion, this study highlighted the involvement of miR-153, miR-28 and miR-708 in regulatory network of Nrf2 mediated antioxidant system in bovine granulosa cells function. Furthermore, quercetin at a low dose played a protective role in bovine granulosa cells against oxidative stress damage.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
• Sadek, I.I., El-Desokey, W.M.S., Taqi, M.O., Moursy F.S. (2014). Monitoring and description of annual accumulative growing degree days changes in Nile Delta governorates. Life science Journal, 11 (6): 655-665
• Hassanein, M. K.; Fahim, M. A.; Khalil, A. A.; Abolmaty, S. M.; Refaie, K. M.; Taqi, M.O. and Abou Hadid, A. F. (2014). Impact of urbanization on agricultural land losses and climate change Case study: Gharbiah Governorate, Egypt. Journal of American Science, 10(1): 99-107
• Taqi, M. O.; Hassanein, M. K.; Khalil, A. A. (2013). The possible impact of climate change and adaptation options on African livestock: A review. Researcher, 5(12): 139-145
• Hassanein, M. K.; Abolmaaty, S. M.; Khalil, A. A.; Taqi, M. O.; Essa, Yassmin H.; and Shawki, H. H. (2013). Geographical Distribution and Developmental Pattern of Buffalo in Egypt. World Rural Observations, 5(4): 17-19
• Abdrabbo M. A.; Farag, A.A.; Abul-Soud, M.; Gad El-Mola Manal, M.H.; Moursy Fatma, S.; Sadek, I. I.; Hashem, F. A.; Taqi, M. O.; El-Desoky, W.M.S. and Shawki, H.H. (2012). Utilization of satellite imagery for drought monitoring in Egypt. World Rural Observations, 4(3): 27-37
• Hafez, Y. M., Taqi, M.O., Baiomy, A.A., Medany, M.A. and Abou-Bakr, S. (2011). Physiological and hormonal responses of Egyptian buffalo to different climatic conditions. Egyptian J. Anim. Prod., 48:61-73
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