Vancomycin-resistant Enterococcus (VRE) isolates from dogs and cats in veterinary hospitals in Brazil Ricardo Antonio Pilegi Sfaciotte, Leandro Parussolo, Fernanda Danielle Melo, Mateus Felipe Schneider, Ubirajara Maciel da Costa, David Germano Gonçalves Schwarz, Fabiano Zanini Salbego, Sandra Maria Ferraz BMC Veterinary Research, 2025 BACKGROUND: In veterinary medicine, particularly concerning dogs and cats, there is limited data regarding vancomycin-resistant Enterococcus (VRE). However, multidrug-resistant Enterococcus is frequently identified, raising concerns about the potential for spreading these resistant microorganisms to humans due to their zoonotic nature. This study aimed to identify VRE colonizing animals admitted to a veterinary hospital and to determine the presence of the major resistance genes responsible for vancomycin resistance. RESULTS: Enterococcus was found to carry the vanA gene in 52.54% of cases, the vanB gene in 23.73%, the vanC gene in 20.34%, and the vanE gene in 3.39%. The antimicrobials with the lowest resistance were chloramphenicol (5.08%) and ampicillin (6.78%). In contrast, the highest resistance was observed with enrofloxacin (79.66%), rifampicin (67.80%), and ciprofloxacin (61.02%). Seven Enterococcus isolates showed resistance to vancomycin as well as high-level aminoglycoside resistance (HLAR). CONCLUSION: A total of 46 animals were found to be colonized by VRE, of which 16 were healthy. The vanA gene was the most commonly isolated (52.54%), followed by vanB (23.73%), vanC (20.34%), and vanE (3.39%). This represents the first report of the vanE gene being identified in a dog in Brazil. Among the 59 Enterococcus isolates, 84.75% (n = 50) were found to be multidrug resistant. The colonization of VRE and Enterococcus HLAR in dogs and cats poses a public health concern, as it increases the risk of environmental dissemination and has implications for One Health.
Phenotypic and Genotypic of Antimicrobial Resistance Profile of Staphylococcus spp. Isolated from Raw Milk and Artisanal Cheese Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz Foodborne Pathogens and Disease, 2025 Staphylococcus aureus and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in Staphylococcus coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (n = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (mecA, mecC, and blaZ) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for mecA, whereas 10 strains (4 CoPS and 6 CoNS) were positive for blaZ. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.
Cross-border surveillance of sheep and goat rabies in Brazil Francisco Alyson Silva Oliveira, Rivanni Jeniffer Souza Castro, Abelardo Silva-Júnior, Raizza Eveline Escócio Pinheiro, Ricardo Antônio Pilegi Sfaciotte, David Germano Gonçalves Schwarz Comparative Immunology Microbiology and Infectious Diseases, 2024
Spread analysis of glanders in the state of Piauí, northeastern Brazil Roselma de Carvalho Moura, Francisco Alyson de Oliveira, Dayseanny de Oliveira Bezerra, Raizza Eveline Escórcio Pinheiro, Ricardo Antônio Pilegi Sfaciotte, Joandes Henrique Fonteque, Sandra Maria Ferraz, Raylson Pereira de Oliveira, Verônica Flores da Cunha Scheeren, David Germano Gonçalves Schwarz Comparative Immunology Microbiology and Infectious Diseases, 2024
Spatial risk assessment of ovine Scrapie in Brazil Paulo Gomes do Nascimento Corrêa, Francisco Alyson Silva Oliveira, Rivanni Jeniffer Souza Castro, Carlos Thiago Silveira Alvim Mendes de Oliveira, Glenda Lídice de Oliveira Cortez Marinho, Abelardo Silva Júnior, Ricardo Antonio Pilegi Sfaciotte, David Germano Gonçalves Schwarz Microbial Risk Analysis, 2023
Causes of fetal death in the Flemish cattle herd in Brazil Lucas Marian, Jéssica Aline Withoeft, Leonardo da Silva Costa, Luiza Ramos Ribeiro, Isadora Cristina Melo, Raquel Silva Alves, Letícia Ferreira Baumbach, Maicon Gaissler Lorena Pinto, Alessandra Snak, Luiz Claudio Miletti, Sandra Maria Ferraz, Ricardo Antônio Pilegi Sfaciotte, Cláudio Wageck Canal, Renata Assis Casagrande Veterinary World, 2023
