Shagufta Perveen
@bact.wisc.edu
Scientist III at Department of Bacteriology
University of Wisconsin - Madison
Dr. Shagufta Perveen, a Pakistani national, Natural Product Chemist/Scientist by profession, born in a religious, modern, well-educated and patriotic family. Her father, Muhammad Kamran Khan is Deputy Manager (R) from civil engineering department of Pakistan Steels Mills Corporation and mother Razia Khanum is a housewife. Since Pakistan came into being, most of her family members were serving Government of Pakistan in different departments such as Pakistan Railway, Accountant General Office, Education, Police, etc.
Dr. Shagufta obtained her early education from her home town Shah Faisal Colony, Karachi and later moved to University of Karachi and graduated in Organic Chemistry with 1st division. After graduation, she got scholarship from Higher Education Commission of Pakistan and joined one of the finest institutes of Pakistan, International Center for Chemical and Biological Sciences (HEJ Research Institute of Chemistry and Dr Panjwani Center for Molecular Medicine and Drug Research), University of Karachi for higher studies in Chemistry and Biology. The ICCBS was governed by world’s well-known scientists including Professor Dr Atta-ur-Rahman a UNESCO, Science Laureate (F.R.S., N.I., H.I., S.I., T.I.) as Patron-in-Chief and his mentee Prof. Dr Muhammad Iqbal Choudhary (H.I., S.I., T.I.) as Director. Dr Shagufta started her PhD in 2003 under supervision of Prof Dr Abdul Malik (R) (S.I.), who was a senior faculty member of ICCBS and also performed duties of acting director in the absence of Director. Dr Shagufta completed Part A of her thesis topic “Phytochemical Investigation on the Chemical Constituents of Perovskia Atriplicifolia” from ICCBS (HEJ), University of Karachi, Pakistan.
At the end of 2006, she got another short-term scholarship and joined Prof. Dr Jeremy D Kilburn (Head of School of Chemistry) at University of Southampton, GB, where she completed Part B of her thesis topic “The Synthesis of Super-molecules”.
EDUCATION
1. 2009 PhD (Natural Product Chemistry / Organic Chemistry / Synthetic Chemistry):
Thesis Part A from the HEJ Institute of Chemistry, University of Karachi, Karachi, Pakistan; under supervision of Professor Dr Abdul Malik, topic "Phytochemical Investigation on the Chemical Constituents of Perovskia Atriplicifolia".
Thesis Part B from the School of Chemistry, the University of Southampton, Southampton, UK; under supervision of Professor Jeremy D Kilburn (Head of School), topic "The Synthesis of Supramolecules".
2. 2002 MSc (Organic Chemistry) from University of Karachi, Karachi, Pakistan.
3. 1998 BSc (Chemistry/Biology/Physics) from University of Karachi, Karachi, Pakistan.
RESEARCH, TEACHING, or OTHER INTERESTS
Multidisciplinary
FUTURE PROJECTS
Beauty and Anti-Bacterial Soap
Applications Invited
Testing & Patent
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Shagufta Perveen, Azadeh Hamedi, Ardalan Pasdaran, Reza Heidari, Muhammad Shafiq ul Azam, Sobia Tabassum, Rashad Mehmood, and Jiangnan Peng
Springer Science and Business Media LLC
Abida Parveen, Shagufta Perveen, Sana Tariq, Muhammad Atif, Farah Saeed, and Sara Zafar
Springer Science and Business Media LLC
Muhammad Atif and Shagufta Perveen
Springer Science and Business Media LLC
Muhammad Hamzah Saleem, Abida Parveen, Shagufta Perveen, Naheed Akhtar, Fozia Abasi, Maria Ehsan, Habib Ali, Mohammad K. Okla, Ibrahim A. Saleh, Naser Zomot,et al.
Springer Science and Business Media LLC
Muhammad Hamzah Saleem, Abida Parveen, Shagufta Perveen, Naheed Akhtar, Fozia Abasi, Maria Ehsan, Habib Ali, Mohammad K. Okla, Ibrahim A. Saleh, Naser Zomot,et al.
Springer Science and Business Media LLC
Fredi Cifuentes, Javier Palacios, Daniel Asunción-Alvarez, Ricardo D. G. de Albuquerque, Mario J. Simirgiotis, Adrián Paredes, Chukwuemeka R. Nwokocha, Raha Orfali, and Shagufta Perveen
Springer Science and Business Media LLC
Ghanwa Riaz, Amna Shoaib, Sidra Javed, Shagufta Perveen, Waseem Ahmed, Mohamed A. El-Sheikh, and Prashant Kaushik
Elsevier BV
Fredi Cifuentes, Javier Palacios, Daniel Asunción-Alvarez, Ricardo D. G. de Albuquerque, Mario J. Simirgiotis, Adrián Paredes, Chukwuemeka R. Nwokocha, Raha Orfali, and Shagufta Perveen
Springer Science and Business Media LLC
Hafiza Sibgha Yaqoob, Amna Shoaib, Aneela Anwar, Shagufta Perveen, Sidra Javed, and Samina Mehnaz
Springer Science and Business Media LLC
Muhammad Saeed, Muhammad Naeem, Alia Javed, Shagufta Perveen, Iqra Sajjad, Muhammad Zaid Yousaf, M. Shahid Munir Chohan, Muhammad Riaz, Sana Ullah, and Xianliang Song
Springer Science and Business Media LLC
Eman Fikry, Raha Orfali, Shaimaa S. El-Sayed, Shagufta Perveen, Safina Ghafar, Azza M. El-Shafae, Maher M. El-Domiaty, and Nora Tawfeek
MDPI AG
Methotrexate (MTX) therapy encounters significant limitations due to the significant concern of drug-induced liver injury (DILI), which poses a significant challenge to its usage. To mitigate the deleterious effects of MTX on hepatic function, researchers have explored plant sources to discover potential hepatoprotective agents. This study investigated the hepatoprotective effects of the ethanolic extract derived from the aerial parts of Chamaecyparis lawsoniana (CLAE) against DILI, specifically focusing on MTX-induced hepatotoxicity. UPLC-ESI-MS/MS was used to identify 61 compounds in CLAE, with 31 potential bioactive compounds determined through pharmacokinetic analysis. Network pharmacology analysis revealed 195 potential DILI targets for the bioactive compounds, including TP53, IL6, TNF, HSP90AA1, EGFR, IL1B, BCL2, and CASP3 as top targets. In vivo experiments conducted on rats with acute MTX-hepatotoxicity revealed that administering CLAE orally at 200 and 400 mg/kg/day for ten days dose-dependently improved liver function, attenuated hepatic oxidative stress, inflammation, and apoptosis, and reversed the disarrayed hepatic histological features induced by MTX. In general, the findings of the present study provide evidence in favor of the hepatoprotective capabilities of CLAE in DILI, thereby justifying the need for additional preclinical and clinical investigations.
Mohammad K. Okla, Muhammad Hamzah Saleem, Ibrahim A. Saleh, Naser Zomot, Shagufta Perveen, Abida Parveen, Fozia Abasi, Habib Ali, Baber Ali, Yasmeen A. Alwasel,et al.
Springer Science and Business Media LLC
AbstractIn the current industrial scenario, cadmium (Cd) as a metal is of great importance but poses a major threat to the ecosystem. However, the role of micronutrient − amino chelates such as iron − lysine (Fe − lys) in reducing Cr toxicity in crop plants was recently introduced. In the current experiment, the exogenous applications of Fe − lys i.e., 0 and10 mg L − 1, were examined, using an in vivo approach that involved plant growth and biomass, photosynthetic pigments, oxidative stress indicators and antioxidant response, sugar and osmolytes under the soil contaminated with varying levels of Cd i.e., 0, 50 and 100 µM using two different varieties of canola i.e., Sarbaz and Pea − 09. Results revealed that the increasing levels of Cd in the soil decreased plant growth and growth-related attributes and photosynthetic apparatus and also the soluble protein and soluble sugar. In contrast, the addition of different levels of Cd in the soil significantly increased the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), which induced oxidative damage in both varieties of canola i.e., Sarbaz and Pea − 09. However, canola plants increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and non-enzymatic compounds such as phenolic, flavonoid, proline, and anthocyanin, which scavenge the over-production of reactive oxygen species (ROS). Cd toxicity can be overcome by the supplementation of Fe − lys, which significantly increased plant growth and biomass, improved photosynthetic machinery and sugar contents, and increased the activities of different antioxidative enzymes, even in the plants grown under different levels of Cd in the soil. Research findings, therefore, suggested that the Fe − lys application can ameliorate Cd toxicity in canola and result in improved plant growth and composition under metal stress.
Muhammad Atif, Shagufta Perveen, Abida Parveen, Sara Zafar, Fatima Batool, and Farah Saeed
Springer Science and Business Media LLC
Khalid Sultan, Shagufta Perveen, Abida Parveen, Muhammad Atif, and Sara Zafar
Springer Science and Business Media LLC
Sidra Hameed, Muhammad Atif, and Shagufta Perveen
Springer Science and Business Media LLC
Khlood Jamal, Areej Al-Taweel, Sarah I. Bukhari, Raha Orfali, Nadine M. S. Moubayed, Jawaher Al-Qahtani, Hanan Aati, Orazio Taglialatela-Scafati, Jiangnan Peng, and Shagufta Perveen
MDPI AG
A phytochemical investigation of the stems of the Arabian plant Artemisia sieberi afforded three new isochlorogenic acid derivatives, namely isochlorogenic acid A-3′-O-β-glucopyranoside (1), isochlorogenic acid A-3′-O-β-glucopyranoside methyl ester (2), and isochlorogenic acid C-3′-O-β-glucopyranoside (3), obtained along with thirteen known secondary metabolites belonging to distinct structural classes. The structures of the new metabolites were elucidated by modern spectroscopic techniues based on high-resolution mass spectrometry (HR-ESIMS) and 1D/2D nuclear magnetic resonance (NMR). All isolated compounds were tested for their potential antimicrobial activity against four different bacterial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), in addition to a fungal strain (Candida tropicalis), The results were expressed as the diameter of the clear zone (in millimetres) around each well. Compounds 1 and 3 (isochlorogenic acid A-3′-O-β-glucopyranoside and isochlorogenic acid C-3′-O-β-glucopyranoside, respectively) displayed remarkable antifungal effect and potent antibacterial activities against B. subtilis and S. aureus, respectively. 3α,4α-10β-trihydroxy-8α-acetyloxyguaian-12,6α-olide (6) and angelicoidenol 2-O-β-d-glucopyranoside (9) emerged as interesting dual antibacterial (selective on P. aeruginosa)/antifungal agents.
Ghanwa Riaz, Amna Shoaib, Sidra Javed, Shagufta Perveen, Waseem Ahmed, Mohamed A. El-Sheikh, and Prashant Kaushik
Elsevier BV
Eman Fikry, Raha Orfali, Samar S. Elbaramawi, Shagufta Perveen, Azza M. El-Shafae, Maher M. El-Domiaty, and Nora Tawfeek
MDPI AG
Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions.
Saifullah Bullo, Rifat Jawaria, Iqra Faiz, Iqra Shafiq, Muhammad Khalid, Muhammad Adnan Asghar, Rabia Baby, Raha Orfali, and Shagufta Perveen
American Chemical Society (ACS)
Currently, we reported the synthesis of six novel salicylaldehyde-based thiosemicarbazones (BHCT1–HBCT6) via condensation of salicylaldehyde with respective thiosemicarbazide. Through various spectroscopic methods, UV–visible and NMR, the chemical structures of BHCT1–HBCT6 compounds were determined. Along with synthesis, a computational study was also performed at the M06/6-31G(d,p) functional. Various analyses such as natural bond orbital (NBO) analysis, natural population analysis, frontier molecular orbital (FMO) analysis, and molecular electrostatic potential surfaces were carried out to understand the nonlinear optical (NLO) characteristics of the synthesized compounds. Additionally, a comparative study was carried out between DFT and experimental results (UV–vis study), and a good agreement was observed in the results. The energy gap calculated through FMOs was found to be in decreasing order as 4.505 (FHCT2) > 4.499 (HBCT6) > 4.497 (BHCT1) = 4.497(HMCT5) > 4.386 (CHCT3) > 4.241(AHCT4) in eV. The global reactivity parameters (GRPs) were attained through EHOMO and ELUMO, which described the stability and hardness of novel compounds. The NBO approach confirmed the charge delocalization and stability of the molecules. Among all the investigated compounds, a larger value (557.085 a.u.) of first hyperpolarizability (βtot) was possessed by CHCT3. The NLO response (βtot) of BHCT1–HBCT6 was found to be 9.145, 9.33, 13.33, 5.43, 5.68, and 10.13 a.u. times larger than that of the standard para-nitroaniline molecule. These findings ascertained the potential of entitled ligands as best NLO materials for a variety of applications in modern technology.
Jia Li, Sara Zafar, Ayesha Javaid, Shagufta Perveen, Zuhair Hasnain, Muhammad Ihtisham, Adeel Abbas, Muhammad Usman, Ahmed H. El-Sappah, and Manzar Abbas
MDPI AG
The detrimental effects of drought have adverse impacts on the crop yield as global climatic changes put unusual pressure on water resources. The challenge of attaining water security is key for the sustainable development of crops. Zinc (Zn2+) is an important nutrient that helps to alleviate drought stress by modulating the growth and yield of crops. Recently, zinc nanoparticles (ZnNPs) have been used as a novel strategy for the fertilization of crops. This study was specifically developed to observe the comparative effects of ZnNPs and conventional zinc sulfate (ZnSO4) at diverse concentration levels (0.01%, 0.05%, and 0.1%) that could effectively decrease the injurious effect of drought stress on turnip plants. In experiments on the golden turnip variety, drought stress caused a significant reduction in all growth and biochemical attributes, and increased antioxidant enzymatic activity. In a comparison with the conventional fertilizer ZnSO4, the foliar application of 0.1% ZnNPs significantly improved plant height, biomass, root/turnip length, turnip diameter, antioxidant defense system, secondary metabolites, and photosynthetic pigments in the leaves under drought stress. Based on the collected results, it is suggested that the foliar application of ZnNPs, instead of ZnSO4, under drought stress is helpful in increasing the growth and yield of turnip plants.
Muhammad Nawaz Tahir, Akbar Ali, Muhammad Khalid, Muhammad Ashfaq, Mubashir Naveed, Shahzad Murtaza, Iqra Shafiq, Muhammad Adnan Asghar, Raha Orfali, and Shagufta Perveen
MDPI AG
Two aminobenzoic acid based crystalline imines (HMBA and DHBA) were synthesized through a condensation reaction of 4-aminobenzoic acid and substituted benzaldehydes. Single-crystal X-ray diffraction was employed for the determination of structures of prepared Schiff bases. The stability of super molecular structures of both molecules was achieved by intramolecular H-bonding accompanied by strong, as well as comparatively weak, intermolecular attractive forces. The comparative analysis of the non-covalent forces in HMBA and DHBA was performed by Hirshfeld surface analysis and an interaction energy study between the molecular pairs. Along with the synthesis, quantum chemical calculations were also accomplished at M06/6-311G (d, p) functional of density functional theory (DFT). The frontier molecular orbitals (FMOs), molecular electrostatic potential (MEP), natural bond orbitals (NBOs), global reactivity parameters (GRPs) and natural population (NPA) analyses were also carried out. The findings of FMOs found that Egap for HMBA was examined to be smaller (3.477 eV) than that of DHBA (3.7933 eV), which indicated a greater charge transference rate in HMBA. Further, the NBO analysis showed the efficient intramolecular charge transfer (ICT), as studied by Hirshfeld surface analysis.
Hasnain Mehmood, Tashfeen Akhtar, Muhammad Haroon, Muhammad Khalid, Simon Woodward, Muhammad Adnan Asghar, Rabia Baby, Raha Orfali, and Shagufta Perveen
American Chemical Society (ACS)
A novel series of fluorophenyl-based thiazoles was synthesized following the Hanztsch method. All of the compounds were initially verified with physical parameters (color, melting point, retardation factor (Rf)), which were further confirmed by several spectroscopic methods, including ultraviolet–visible (UV–visible), Fourier-transform infrared (FTIR), 1H, 13C, 19F NMR, and high-resolution mass spectrometry (HRMS). The binding interactions of all compounds were studied using a molecular docking simulation approach. Furthermore, each compound was evaluated for its alpha(α)-amylase, antiglycation, and antioxidant potentials. The biocompatibility of all compounds was checked with an in vitro hemolytic assay. All synthesized scaffolds were found biocompatible with minimal lysis of human erythrocytes as compared to the standard Triton X-100. Among the tested compounds, the analogue 3h (IC50 = 5.14 ± 0.03 μM) was found to be a highly potent candidate against α-amylase as compared to the standard (acarbose, IC50 = 5.55 ± 0.06 μM). The compounds 3d, 3f, 3i, and 3k exhibited excellent antiglycation inhibition potential with their IC50 values far less than the standard amino guanidine (IC50 = 0.403 ± 0.001 mg/mL). The antidiabetic potential was further supported by docking studies. Docking studies revealed that all synthesized compounds exhibited various interactions along enzyme active sites (pi–pi, H-bonding, van der Waals) with varied binding energies.
Muhammad Haroon, Tashfeen Akhtar, Muhammad Khalid, Hasnain Mehmood, Muhammad Adnan Asghar, Rabia Baby, Raha Orfali, and Shagufta Perveen
Royal Society of Chemistry (RSC)
Solar energy being a non-depleting energy resource, has attracted scientists' attention to develop efficient solar cells to meet energy demands.
Vikas Kumar, Nitin Sharma, Raha Orfali, Chirag N. Patel, Radwan Alnajjar, Rakshandha Saini, Anuradha Sourirajan, Prem Kumar Khosla, Kamal Dev, and Shagufta Perveen
MDPI AG
Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide. Terminalia arjuna (Roxb. ex DC.) Wight & Arnot of the Combretaceae family is one of the most frequently approved and utilized medicinal trees in the traditional medicinal system, which was used for the treatment of a variety of diseases, including cardiovascular disorders. The present study aims to identify phytochemicals from T. arjuna, that do not exhibit any toxicity and have significant cardioprotective activity using an in-silico technique. Four different cardiovascular proteins, namely human angiotensin receptor (PDB ID: 4YAY), P38 mitogen-activated protein kinase (MAPK, PDB ID: 4DLI), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-Co A) reductase (PDB ID: 1HW9), and human C-reactive protein (PDB ID: 1B09), were used as target proteins to identify potential inhibitors using a virtual screening of the phytochemicals in T. arjuna revealed casuarinin as a potential inhibitor of all selected target proteins with strong binding energy. Furthermore, MD simulations for a 100 ns time scale also revealed that most of the key protein contacts of all target proteins were retained throughout the simulation trajectories. Binding free energy calculations using the MM-GBSA approach also support a strong inhibitory effect of casuarinin on target proteins. Casuarinin’s effective binding to these proteins lays the groundwork for the development of broad-spectrum drugs as well as the understanding of the underlying mechanism against cardiovascular diseases through in vivo and clinical studies.
Abdul Basit, Saeed Ahmad, Kashif ur Rehman Khan, Hanan Y. Aati, Asmaa E. Sherif, Chitchamai Ovatlarnporn, Safiullah Khan, Huma Rao, Muhammad Adeel Arshad, Muhammad Nadeem Shahzad,et al.
Frontiers Media SA
Introduction:Cardamine amara L. (Brassicaceae) is an important edible plant with ethnomedicinal significance. This study aimed at evaluating the phytochemical composition, anti-inflammatory, antioxidant and cytotoxicity aspects of the hydro-alcoholic extract of C. amara (HAECA).Methods: The phytochemical composition was evaluated through total phenolic contents (TPC), total flavonoid contents (TFC) determination and UPLC-QTOF-MS profiling. Anti-inflammatory evaluation of HAECA was carried out through the carrageenan induced paw edema model. Four in vitro methods were applied in the antioxidant evaluation of HAECA. MTT assay was used to investigate the toxicity profile of the species against human normal liver cells (HL7702), human liver cancer cell lines (HepG2) and human breast cancer cell lines (MCF-7). Three major compounds (Gentisic acid, skullcapflavone and conidendrine) identified in UPLC-Q-TOF-MS analysis were selected for in silico study against cyclooxygenase (COX-I and COX-II).Results and Discussion: The findings revealed that HAECA is rich in TPC (39.32 ± 2.3 mg GAE/g DE) and TFC (17.26 ± 0.8 mg RE/g DE). A total of 21 secondary metabolites were tentatively identified in UPLC-Q-TOF-MS analysis. In the MTT cytotoxicity assay, the extract showed low toxicity against normal cell lines, while significant anticancer activity was observed against human liver and breast cancer cells. The carrageenan induced inflammation was inhibited by HAECA in a dose dependent manner and showed a marked alleviation in the levels of oxidative stress (catalase, SOD, GSH) and inflammatory markers (TNF-α, IL-1β). Similarly, HAECA showed maximum antioxidant activity through the Cupric reducing power antioxidant capacity (CUPRAC) assay (31.21 ± 0.3 mg TE/g DE). The in silico study revealed a significant molecular docking score of the three studied compounds against COX-I and COX-I. Conclusively the current study encourages the use of C. amara as a novel polyphenolic rich source with anti-inflammatory and antioxidant potential and warrants further investigations on its toxicity profile.