Harnessing endophytes for enhancing the production of valuable plant secondary metabolites Vrishali Rajendra Bankar, Shilpa Samir Chapadgaonkar Natural Resources for Human Health, 2025 The vast diversity of plant species on Earth harbors an equally rich and untapped reservoir of microbial endophytes, offering immense potential for sustainable development and biotechnological innovations. The plant microbiome is a key regulator of plant secondary metabolic pathways. This interaction could be exploited to increase the production of phytochemicals of commercial interest. These phytochemicals have diverse applications in industry, agriculture, and pharmaceuticals. The growing potential of endophytes as bio-factories for synthesizing bioactive phytochemicals is particularly intriguing. Recent breakthroughs in host-independent metabolite synthesis by endophytes present an interesting alternative to over harvesting endangered medicinal plants. The examples of host-free metabolite production and bioprocessing techniques for harnessing the endophytes to improve plant metabolite production have been thoroughly examined. This review sheds light on the omics technologies poised to transform endophyte-plant interactome research and its potential applications.
Enzyme engineering of choline oxidase for improving stability Sonia Kaushik, Rashmi Rameshwari, Shilpa S. Chapadgaonkar Journal of Biomolecular Structure and Dynamics, 2025 Functioning as a flavoprotein, choline oxidase facilitates the transformation of choline into glycine betaine. Notably, choline oxidase and its resultant product, glycine betaine, find extensive applications across various industries and fields of study. However, its high sensitivity and tendency to lose functional activity at high temperatures reduces its industrial usage. MD simulation and mutation studies have revealed the role of certain residues responsible for the enzyme's thermal instability. This study focuses on inducing thermal stability to choline oxidase of A. globiformis through computational approaches at a maximum temperature of 60 °C. MD simulation analysis showed that Trp 331, Val 464 and Ser 101 contribute to structural instability, leading to the instability at 60 °C. Mutation of these residues with phenylalanine residues and simulation of the mutated enzyme at 60 °C exhibited thermostability and insignificant residual fluctuation. The re-docking and MM/GBSA analyses further validated the mutated enzyme's binding affinity and catalytic activity.Communicated by Ramaswamy H. Sarma.
Cosmeceuticals from the Sea Handbook of Research in Marine Pharmaceutics Exploring Oceanic Microbial Diversity for Human Health and Wellness, 2025
Harnessing the Untapped Potential of Cold-Adapted Enzymes Shilpa S. Chapadgaonkar, Bidisha Borah Das, Abhilasha Shourie Industrial Biotechnology, 2024 Cold-adapted enzymes exhibit high catalytic activity at low temperatures and specificity, making them ideal biocatalysts for various industrial applications. Their thermolability facilitates easy inactivation under mild conditions, preventing excessive enzymatic activity. In the detergent industry, cold-adapted proteases, lipases, and amylases enable effective stain removal and washing at low temperatures, reducing energy consumption. The textile industry benefits from cold-adapted cellulases and amylases for fabric processing and surface modification. In the food industry, these enzymes enhance processes like meat tenderization, fruit juice clarification, and lactose removal in dairy products, ensuring better preservation and quality at lower temperatures. Cold-adapted enzymes are also vital in the pharmaceutical industry, aiding in peptide synthesis, wound healing, and antiviral treatments. Their role extends to agriculture, where they enhance soil fertility, plant growth, and biocontrol of pathogens, and to bioremediation, where they degrade pollutants in cold environments. Additionally, cold-adapted enzymes are used in the production of fine chemicals, such as biodiesel and chiral intermediates, and as tools in research for processes like DNA manipulation. Their unique properties offer significant advantages across diverse fields, driving innovation and sustainability in industrial processes. This review discusses promising new applications of cold-adapted enzymes in diverse industrial sectors and the strategies to overcome the present upstream and downstream processing challenges including in the application of cold-adapted enzymes.
The in-silico study of the structural changes in the Arthrobacter globiformis choline oxidase induced by high temperature Sonia Kaushik, Rashmi Rameshwari, Shilpa S. Chapadgaonkar Journal of Genetic Engineering and Biotechnology, 2024 BACKGROUND: Choline oxidase, a flavoprotein, is an enzyme that catalyzes the reaction which converts choline into glycine betaine. Choline oxidase started its journey way back in 1933. However, the impact of the high temperature on its structure has not been explored despite the long history and availability of its crystal structure. Both choline oxidase and its product, glycine betaine, have enormous applications spanning across multiple industries. Understanding how the 3D structure of the enzyme will change with the temperature change can open new ways to make it more stable and useful for industry. PROCESS: This research paper presents the in-silico study and analysis of the structural changes of A. globiformis choline oxidase at temperatures from 25 °C to 60 °C. A step-wise process is depicted in Fig. 1. RESULTS: was considered for molecular dynamics simulations. A simulated annealing option was used to gradually increase the temperature of the system from 25 °C to 60 °C. Analysis of the conserved residues, as well as residues involved in Flavin Adenine Dinucleotide (FAD) binding, substrate binding, substate gating, and dimer formationwas done. At high temperatures, the formation of the inter-chain salt bridge between Arg50 and Glu63 was a significant observation near the active site of choline oxidase. CONCLUSION: Molecular dynamics studies suggest that an increase in temperature has a significant impact on the extended Flavin Adenine Dinucleotide (FAD) binding region. These changes interfere with the entry of substrate to the active site of the enzyme and make the enzyme inactive.
Biocontaminants in Indoor Environments: Occurrence, Spread, and Prevention Abhilasha Shourie, Anita Girdhar, Kriti Singhal, Chanchal Rawat, Shilpa Samir Chapadgaonkar Airborne Biocontaminants and their Impact on Human Health, 2024 Biocontaminants is a broad term that encompasses a panorama of toxicants, microbes, and other pollutants of biological origin, including viruses, fungi, bacteria, algae, mites, insects, and pollen grains. Their origin can be traced through their sources that are highly diverse and comprise articles and materials in human vicinity such as home furnishings, air conditioners (ACs), humidifiers, kitchen surfaces, bathroom objects and surfaces, household pets, construction sites, building materials, dust particles, plants, and many more. Biocontaminants, being omnipresent, have a profound impact on the environment and human health as they cause several types of communicable or noncommunicable diseases and other ailments that compromise the quality of human life and in certain instances lead to severe morbidity and mortality. Indoor confinement of humans has not only increased the biocontaminants in the microenvironments but has also contributed to the enhanced burden of diseases. The chapter gives an overview of the occurrence and types of biocontaminants in various microenvironments, factors that are instrumental in their build-up, and conditions supporting their spread within and across various indoor microenvironments. The chapter also proposes various measures that would help restrict the spread and sustenance of biocontaminants and prevent related diseases in indoor occupants.
Nanomaterials mediated and biosorption-based photocatalytic treatment for efficient and rapid bioremediation of textile industry wastewater Pooja Sharma, Vishal Virole, Tauseef Anwar, Om Kolekar, Anup A. Kale, Shilpa Chapadgaonkar, Ashish Yengantiwar Proceedings of SPIE the International Society for Optical Engineering, 2024 The textile industry significantly contributes to toxic wastewater generation, with processing steps consuming large volumes of water and releasing hazardous dyes. These dyes, with complex aromatic structures, are stable and challenging to remove using conventional on-site treatments. Herein, the present work investigates the comparative effectiveness of various techniques for treating textile dye wastewater, including biosorption using agricultural waste, bioremediation using microbial consortia, and photocatalytic degradation. A simulated textile waste has been coated with commercial blue dye and further treated with synthesised nanoparticles of bismuth vanadate (BiVO<sub>4</sub>). It shows an effective activity of about 65% dye degradation within 1 hour of treatment. The residual dye has adsorbed in a packed bed reactor (PBR) with different agricultural waste biomass such as rice stalk, peanut hulls, sawdust. The adsorption led to more than 80% removal of textile dyes from the dye solution. The bioremediation process has carried out to degrade the residual dyes eluting from the biomass PBR and the dye recovered by solvent from the biomass adsorbent. It has observed that the dye containing wastewater eluted from the PBR and completely degraded by microbial degradation within 24-72 hours of culture. Based on these findings, we propose a combined approach of nanomaterials enabled photocatalysis and biosorption that leverages the strengths of each method, breakdown of toxicants via photocatalysis coupled with the microbial degradation and biosorption to completely remove toxicants for releasing safe water from textile industry.
Thermo-alkali stable bacterial xylanase for deinking of copier paper Girisha Malhotra, Shilpa S. Chapadgaonkar Journal of Genetic Engineering and Biotechnology, 2023 BACKGROUND: The bleach-boosting capability of xylanases is well-known. The use of xylanase pre-treatment before the application of chemical bleach has multiple advantages including (i) lesser use of polluting chemicals of the traditional bleaching process; (ii) less damage to the cellulosic fibers, therefore better recyclability; and (iii) better brightness of chemical bleach. The major impediment in the application is the availability of commercial enzymes that are active at the elevated temperature and pH that exist during the industrial pulping process. In the present paper, xylanase having suitability for application in deinking is reported. RESULTS: The xylanase used showed high deinking potential. Optimal deinking was obtained at the xylanase dosing of 20U/g of the dried pulp at 60℃ for a treatment time of 1h. It could bring about a 50% reduction in the usage of chemical bleach that was applied after xylanase pre-treatment. The comparison of FTIR spectra showed changes in intensity without significant changes in the functional group signatures implying that there is negligible damage to the fiber strength in the xylanase pre-treatment process as compared to the chemical bleach process. CONCLUSION: The xylanase used in this study was effective in deinking paper pulp and can be used for bio-bleaching of recycled paper.
Enzyme engineering of choline oxidase for improving stability S Kaushik, R Rameshwari, SS Chapadgaonkar Journal of Biomolecular Structure and Dynamics 43 (11), 5593-5605 , 2025 2025 Citations: 3
Combinatorial Approach Integrating Advanced Oxidation Process to the Microalgal Treatment for PPCP Removal from Wastewater A Shourie, A Girdhar, S Chapadgaonkar, S Mazahar, A Singh Advanced Oxidation Process-Based Integrated and Hybrid Technologies for … , 2025 2025
Adaptive deep CNN: an effective Alzheimer’s affected MRI image registration using heuristic-aided deep learning model and patch-based level fusion V Deshmukh, S Chapadgaonkar, M Kowdiki, A Khaparde Pattern Analysis and Applications 28 (2), 67 , 2025 2025 Citations: 2
Cosmeceuticals from the Sea S Polshettiwar, SS Chapadgaonkar, D Kannur, V Musale, S Kabra Handbook of Research in Marine Pharmaceutics, 435-446 , 2025 2025 Citations: 2
Advanced Oxidation Process-based Integrated and Hybrid Technologies for Degradation of Pharmaceuticals and Personal Care Products R Kumar, NA Khan, AH Khan, R Shankar, N Pervez Elsevier , 2025 2025 Citations: 2
Harnessing endophytes for enhancing the production of valuable plant secondary metabolites VR Bankar, SS Chapadgaonkar Natural Resources for Human Health 5 (4), 686-704 , 2025 2025 Citations: 3
Harnessing the untapped potential of cold-adapted enzymes SS Chapadgaonkar, BB Das, A Shourie Industrial Biotechnology 20 (6), 257-267 , 2024 2024 Citations: 3
Nanomaterials mediated and biosorption-based photocatalytic treatment for efficient and rapid bioremediation of textile industry wastewater P Sharma, V Virole, T Anwar, O Kolekar, AA Kale, SS Chapadgaonkar, ... Second International Conference on Current Trends in Physics and Photonics … , 2024 2024 Citations: 3
Biocontaminants in Indoor Environments: Occurrence, Spread, and Prevention A Shourie, A Girdhar, K Singhal, C Rawat, SS Chapadgaonkar Airborne Biocontaminants and Their Impact on Human Health, 1-25 , 2024 2024 Citations: 5
SWASTIIK Technology for Drinking Water Treatment-“Enhancing Water Disinfection Efficacy using Fennel Oil and Hydrodynamic Cavitation” SN Berde, D Dixit, SS Chapadgaonkar, VM Bhandari JOURNAL OF ISAS 3 (1), 62-82 , 2024 2024 Citations: 1
The in-silico study of the structural changes in the Arthrobacter globiformis choline oxidase induced by high temperature S Kaushik, R Rameshwari, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 22 (1), 100348 , 2024 2024 Citations: 2
Thermo-alkali stable bacterial xylanase for deinking of copier paper G Malhotra, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 21 (1), 107 , 2023 2023 Citations: 10
Phyto-microbiome to Mitigate Abiotic Stress in Crop plants A Singh, S Mazahar, SS Chapadgaonkar, P Giri, A Shourie Frontiers in Microbiology 14, 1210890 , 2023 2023 Citations: 94
An attention-based swin u-net-based segmentation and hybrid deep learning based diabetic retinopathy classification framework using fundus images A Khaparde, S Chapadgaonkar, M Kowdiki, V Deshmukh Sensing and Imaging 24 (1), 20 , 2023 2023 Citations: 23
Gut microbiome influences incidence and outcomes of breast cancer by regulating levels and activity of steroid hormones in women SS Chapadgaonkar, SS Bajpai, MS Godbole Cancer reports (Hoboken, NJ), e1847 , 2023 2023 Citations: 28
Potential Rational Methods For Improving Thermostability Of Choline Oxidase S Kaushik, R Rameshwari, SS Chapadgaonkar Journal For Basic Sciences/Fangzhi Gaoxiao Jichukexue Xuebao 23 (1), 928-950 , 2023 2023 Citations: 2
Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser G Malhotra, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 20 (1), 50 , 2022 2022 Citations: 8
Choline oxidase: An enzyme of immense industrial potential S Kaushik, R Rameshwari, SS Chapadgaonkar Asia Pacific Journal of Molecular Biology and Biotechnology 30 (1), 37-50 , 2022 2022 Citations: 2
Preparation of nanoparticles incorporated green paper using organic waste. CSS Mitra, Sanchita, Yuktika Research Journal of Chemical Sciences 11 (3), 33-38 , 2021 2021
Optimization of a-Amylase Production from Bacillus Amyloliquefaciens using Taguchi Method P Sharma, S Chapadgaonkar Biosciences Biotechnology Research Asia 18 (2), 337-345 , 2021 2021 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Phyto-microbiome to Mitigate Abiotic Stress in Crop plants A Singh, S Mazahar, SS Chapadgaonkar, P Giri, A Shourie Frontiers in Microbiology 14, 1210890 , 2023 2023 Citations: 94
Chemically modified papain for applications in detergent formulations SS Khaparde, RS Singhal Bioresource technology 78 (1), 1-4 , 2001 2001 Citations: 81
Production and applications of xylanases–an overview G Malhotra, SS Chapadgaonkar BioTechnologia. Journal of Biotechnology Computational Biology and … , 2018 2018 Citations: 38
Alcohol production from fruit and vegetable waste C Shilpa, G Malhotra International Journal of Applied Engineering Research 8 (15), 1749-1756 , 2013 2013 Citations: 37
Synthesis, regulation and production of urokinase using mammalian cell culture: a comprehensive review PK Roychoudhury, SS Khaparde, B Mattiasson, A Kumar Biotechnology advances 24 (5), 514-528 , 2006 2006 Citations: 34
Taguchi optimization and scale up of xylanase from Bacillus licheniformis isolated from hot water geyser G Malhotra, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 18 (1), 65 , 2020 2020 Citations: 29
Gut microbiome influences incidence and outcomes of breast cancer by regulating levels and activity of steroid hormones in women SS Chapadgaonkar, SS Bajpai, MS Godbole Cancer reports (Hoboken, NJ), e1847 , 2023 2023 Citations: 28
Homozygosity and heterozygosity of the pericentric inversion of chromosome 9 and its clinical impact. M KUMAR, A THATAI, SS CHAPADGAONKAR Journal of Clinical & Diagnostic Research 6 (5) , 2012 2012 Citations: 28
An attention-based swin u-net-based segmentation and hybrid deep learning based diabetic retinopathy classification framework using fundus images A Khaparde, S Chapadgaonkar, M Kowdiki, V Deshmukh Sensing and Imaging 24 (1), 20 , 2023 2023 Citations: 23
Optimization of lipase production medium for a bacterial isolate S Dhiman, SS Chapadgaonkar Inter J Chem Technol Res 5, 2837-2843 , 2013 2013 Citations: 21
Production of thermo-alkali-stable xylanase from Bacillus licheniformis isolated from natural hot water geyser G Malhotra, SS Chapadgaonkar Journal of Microscopy and Ultrastructure 9 (3), 131-135 , 2021 2021 Citations: 15
Phytoremediation of indoor pollution–a mini review P Sharma, PC Toma, SS Chapadgaonkar World J Pharma Res 8, 2136-2143 , 2019 2019 Citations: 12
Thermo-alkali stable bacterial xylanase for deinking of copier paper G Malhotra, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 21 (1), 107 , 2023 2023 Citations: 10
Gender bias in autism spectrum disorders-a review A Baruah, K Singla, P Narwat, N Das, SS CHAPADGAONKAR behaviour 19, 20 , 2018 2018 Citations: 9
Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser G Malhotra, SS Chapadgaonkar Journal of Genetic Engineering and Biotechnology 20 (1), 50 , 2022 2022 Citations: 8
Decolourization of Yamuna water using peanut hull in packed bed reactor V Panchal, A Ghosh, PC Tomar, SS Chapadgaonkar Rasayan Journal of Chemistry 13 (2), 949-954 , 2020 2020 Citations: 8
Effect of low culture temperature on urokinase production in hollow fiber reactor SS Khaparde, PK Roychoudhury Applied biochemistry and biotechnology 168 (6), 1655-1663 , 2012 2012 Citations: 7
Production of urokinase using hollow fiber bioreactor SS Khaparde, PK Roychoudhury Indian Chemical Engineering Conference, Technical Session Transcription 6, 11 , 2004 2004 Citations: 7
Aqueous two phase purification of xylanase obtained from a bacterial isolate G Malhotra, R Jeshreena, SS Chapadgaonkar Int J Pharma Bio Sci 7, 305-308 , 2016 2016 Citations: 6
External modulation of HT‐1080 human fibrosarcoma cells improves urokinase production SS Khaparde, PK Roychoudhury, J Gomes, A Mukhopadhyay Biotechnology progress 24 (6), 1325-1332 , 2008 2008 Citations: 6