Prof Shiv Kant Shukla
@kaushalyaskilluniversity.ac.in
Professor & Head
Kaushalya The Skill University
EDUCATION
PhD Biotechnology
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Multidisciplinary
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Susmita Shukla, Subhankar Das, Sparsh Phutela, Ayushi Triathi, Chanchal Kumari, and Shiv Kant Shukla
Springer Nature Switzerland
Ritupriya Singh, Shiv Kant Shukla, and Susmita Shukla
A and V Publications
The prevalence of antibiotic resistance has necessitated the identification of effective drug candidates derived from natural sources and plants. Citrus macropterais an endangered species belonging to the family Rutaceae. The current study focuses on the preliminary screening of the extracts from various parts of C. macroptera and antimicrobial activity assessment against two bacterial strains (Pseudomonas geniculate and Lysinbacillus fusiformis) and two fungal strains (Fusarium oxysporum and Paecilomyceslilacinus). The phytoconstituents such as alkaloids, terpenoids, glycosides, terpenoids etc. were present in varying solvent extracts. The ethanolic and methanolic leaf extracts of C.macroptera showed maximum inhibition against Lysinibacillus fusiformis with inhibition percentages (68.75% and 43.75%) respectively and no inhibition was recorded in C. macroptera aqueous leaf extract.The antifungal activity of the C. macroptera extracts was found maximum in the ex vitro methanolic leaf extract followed by albedo methanolic extract and in vitro ethanolic leaf extract whereas the least activity was recorded in the aqueous leaf extract. The extracts could not develop effective inhibition against Pseudomonas geniculate and Paecilomyces lilacinus. It can be concluded that extracts from C.macroptera have antimicrobial effects against some pathogens implying that they may have prospective applications in the pharmaceutical industry.
Ritupriya Singh, Susmita Shukla, Shiv Kant Shukla, and Deepshikha Pande Katare
Horizon E-Publishing Group
The main component of drug production and formulation, with minimal to no adverse effects, is plant-based medicine. Citrus macroptera is an endangered species of North-Eastern India and has a diverse array of pharmaceutical applications owing to the presence of several bioactive compounds. The study aimed to evaluate the presence of bioactive metabolites, antioxidants, and cytotoxic mechanisms of the in vitro and ex-vitro leaf extracts of C.macroptera. Gas chromatography–mass spectrometry (GC-MS) profiling was performed using the ethanolic leaf extracts. Methanolic and aqueous extracts were profiled using High-performance thin-layer chromatography (HPTLC). Moreover, the cytotoxicity of the methanolic leaf extracts was assessed using the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test against SH-SY5Y, human neuroblastoma cell lines. GC-MS analysis revealed the existence of numerous pharmacologically significant components such as esters, hydrocarbons, fatty alcohols, and ester compounds. The presence of flavonoids and stigmasterol was exhibited by the HPTLC profile. The in-situ HPTLC analysis revealed white fluorescence after derivatization under white light that explicitly confirms the antioxidant potential of the extracts and can serve as a lead source of natural antioxidants. The cytotoxic efficacy of the extracts was observed in a concentration-dependent manner. However, the in vitro leaf extract displayed better suppressive effects against the SH-SY5Y cells with a 24-h IC50 of 167.71?g/mL compared to the ex-vitro leaf extract. Further, a comprehensive study is necessary to identify the cytotoxic compounds and their mode of action. The outcome of our investigation provides empirical evidence that phytoconstituents present in the leaf extracts of C. macroptera can be used as an effective pharmaceutical agent to combat several diseases.
Susmita Shukla and Shiv Kant Shukla
Elsevier
Ritupriya Singh, Susmita Shukla, and Shiv Kant Shukla
Diva Enterprises Private Limited
Susmita Shukla, Umme Aiba, Ritambhara Bhutani, Shiv Kant Shukla, and Anagbogu Florence Chinyere
Elsevier
Susmita Shukla, Ritupriya Singh, Ritambhara Bhutani, Ayushi Tripathi, and Shiv Kant Shukla
Springer Nature Singapore
Ritambhara, Shiv Kant Shukla, and Susmita Shukla
Springer International Publishing
Ritambhara Bhutani, ShivKant Shukla, and Susmita Shukla
Springer Science and Business Media LLC
V. Gupta, S.K. Shukla, S.M. Shrivastav, S. Shukla, K. Kumar, D.P. Saxena, B. Shrivastav, and M. Chaudhary
Science Alert
Susmita Shukla, S. K. Shukla, and S. K. Mishra
Springer Science and Business Media LLC