Anticancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) capped silver and zinc oxide nanoparticles and its molecular characterization Zabin K. Bagewadi, Gouri H. Illanad, T. M. Yunus Khan, Shaik Mohamed Shamsudeen, Sikandar I. Mulla Bioresources and Bioprocessing, 2025 The current investigation reports anti-cancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) and L-Glutaminase capped nanoparticles. The highest L-Glutaminase production of 9.57 U/mL was achieved on the 4th day of fermentation when L-Glutamine was used as the sole carbon and nitrogen source. Enhanced recycling stability was observed after 6 cycles using L-Glutaminase immobilized in 3% agar and agarose matrices. Free and immobilized L- Glutaminase showed K m of 13.89 ± 0.8 and 7.13 ± 0.3 mM and V max of 18.40 ± 1.5 and 24.21 ± 1.7 U/mg respectively. L- Glutaminase capped silver (AgNP) and zinc oxide (ZnONP) nanoparticles were synthesized and structurally characterized using UV visible spectroscopy, FTIR, SEM–EDS, XRD and AFM. L- Glutaminase capped AgNP and ZnONP exhibited good thermal stability with five and three stages weight loss pattern respectively based on TGA. L-Glutaminase capped AgNP exhibited highest inhibitory activity against B. subtilis (45 $$\\pm$$ ± 0.5 mm) and E. coli (33 $$\\pm$$ ± 0.8 mm) whereas, L-Glutaminase capped ZnONP demonstrated highest inhibition against E. coli (30 $$\\pm$$ ± 0.3 mm) and B. cereus (25 $$\\pm$$ ± 0.5 mm). Increased nanoparticles concentration exhibited increased inhibitory potential as compared to wild L-Glutaminase and lowest MIC of 0.09 µg/mL was exhibited against B. cereus. L-Glutaminase capped nanoparticles demonstrated significant antioxidant properties through in-vitro ABTS and DPPH radical scavenging assays in a dosage-dependent manner. L-Glutaminase and capped AgNP and ZnONP, demonstrated pronounced cell cytotoxicity against MCF-7 cancerous cell line with 57.17 µg/mL, 8.13 µg/mL and 28.31 µg/mL IC50 values respectively, suggesting promising properties as anticancer agents in enzyme-based therapy. The results reveal promising biological activities with potential applications in healthcare sector. Graphical Abstract
Computational Screening of Selected Bioactive Phytochemicals Against α-Amylase with Drug-Likeness Evaluation SI Mulla, R Madapathy, K Gouthami, VD Reddy, ZK Bagewadi, AA Khan, ... In Silico Research in Biomedicine, 100286 , 2026 2026
HR-LCMS Metabolic Profiling from Kalanchoe pinnata for Tumor Suppression and Therapeutic Bioactivities by In-vitro and Integrated Network Pharmacology, Molecular Docking and … RY Halayal, ZK Bagewadi, VS Patil, PB Kumbar, DA Yaraguppi, SI Mulla In Silico Research in Biomedicine, 100291 , 2026 2026
In-Silico analysis, molecular docking and dynamic simulation of anti-cancer metabolites derived from Bacillus velezensis ZK Bagewadi, DA Yaraguppi, SI Mulla, VS Patil, MM Naik Medicine in Microecology, 100165 , 2026 2026 Citations: 1
Optimized solar desalination: integrating nanofluids, TiO2-coated basins, and neural network prediction HS Lisboa, VRS Nascimento, ARC da Silva, ITF de Resende, ... Solar Energy 299, 113744 , 2025 2025 Citations: 4
The phytochemicals present in Vitex negundo leaf extract transcriptionally regulates colorectal cancer: An In vitro study using HCT-116 cells K Gouthami, ZK Bagewadi, SI Mulla, S Maity, AH Shaik, H Reddyvari, ... Indian Journal of Biochemistry and Biophysics (IJBB) 62 (9), 1015-1029 , 2025 2025
Optimization of lipid production in Tetradesmus dimorphus using response surface methodology using tannery wastewater for biodiesel production A Devi, V Singh, A Raj, GD Saratale, LFR Ferreira, SI Mulla, ... Bioresource Technology 430, 132585 , 2025 2025 Citations: 2
Green Technologies for Sustainable Wastewater Management S Bharti, GD Saratale, LFR Ferreira, RL de Souza, SI Mulla, A Raj, ... Recent Advances in Bioremediation and Phytoremediation, 93-121 , 2025 2025 Citations: 1
Current strategy of non-model-based bioprocess optimizations with genetic algorithms in bioscience-A systematic review R Surma, D Wojcieszyńska, SI Mulla, U Guzik Computers in Biology and Medicine 192, 110247 , 2025 2025 Citations: 7
Microbial naringinase: from microbial source to its current applications in various fields DB Golgeri M, SI Mulla, ZK Bagewadi, B Faniband, P Mishra, PO Bankole, ... Biologia 80 (4), 977–991 , 2025 2025 Citations: 5
Anticancer, antioxidant and antibacterial potential of L-Glutaminase ( Streptomyces roseolus strain ZKB1) capped silver and zinc oxide nanoparticles and its … ZK Bagewadi, GH Illanad, TMY Khan, SM Shamsudeen, SI Mulla Bioresources and Bioprocessing 12 (1), 23 , 2025 2025 Citations: 2
A critical review on the symbiotic effect of bacteria and microalgae on treatment of sewage with biofertilizer production S Bharti, A Raj, GD Saratale, LFR Ferreira, RL de Souza, SI Mulla, ... Journal of Environmental Management 373, 123704 , 2025 2025 Citations: 11
Distinctive toxic repercussions of polystyrene nano plastic towards aquatic non target species Nitrobacter vulgaris, Scenedesmus sp and Daphnia magna SS Nagaraja, Y Gouda, D Miguez, Y Muralidaran, LF Romanholo Ferreira, ... Ecotoxicology 34 (1), 61-75 , 2025 2025 Citations: 5
Closing the sustainability loop: CuO-infused antibacterial cellulose-dominant matrices for multi-tasking wastewater clean-up and energy storage SV Kamath, V Ankalgi, JR Attokkaran, SI Mulla, K Hegde, AS Maraddi, ... Sustainable Materials and Technologies 41, e01012 , 2024 2024 Citations: 2
Development of a simple biogas analyzer module (BAM) for real-time biogas production monitoring L de Melo, IA Cruz, LT Fuess, CE Maynard Santana, RN Bharagava, ... Environmental Technology 45 (19), 3877-3887 , 2024 2024 Citations: 2
Sustainable Algal Industrial Wastewater Treatment: Applications and Challenges A Devi, C Saran, GD Saratale, RG Saratale, LFR Ferreira, SI Mulla, ... Microbes based approaches for the management of hazardous contaminants, 190-205 , 2024 2024 Citations: 3
Bacterial Treatment of Industrial Wastewaters: Applications and Challenges C Saran, A Devi, GD Saratale, RG Saratale, LFR Ferreira, SI Mulla, ... Microbes Based Approaches for the Management of Hazardous Contaminants, 171-189 , 2024 2024 Citations: 1
Micro and nanoplastics pollution: sources, distribution, uptake in plants, toxicological effects, and innovative remediation strategies for environmental sustainability D Kumar, JK Biswas, SI Mulla, R Singh, R Shukla, MA Ahanger, ... Plant Physiology and Biochemistry 213, 108795 , 2024 2024 Citations: 78
Nanotechnology for removal of personal care products and related compounds RKB Sakappa, SI Mulla, B Faniband, DB Golgeri M., LFR Ferreira, ... Nanotechnology to Monitor, Remedy, and Prevent Pollution A volume in Micro … , 2024 2024 Citations: 1
Regeneration and reusability of non-conventional low-cost adsorbents to remove dyes from wastewaters in multiple consecutive adsorption–desorption cycles: a review N El Messaoudi, M El Khomri, A El Mouden, A Bouich, A Jada, A Lacherai, ... Biomass conversion and biorefinery 14 (11), 11739-11756 , 2024 2024 Citations: 207
The interaction between chromophoric dissolved organic matter and xenobiotics (bisphenols and perfluoroalkyl substances) identified by UV-vis absorption and FT-IR spectroscopy BE Ifon, F Suanon, C Kiki, PO Peter, VD Wotto, D Mama, SI Mulla, CP Yu, ... Journal of Molecular Structure 1295, 136762 , 2024 2024 Citations: 7
MOST CITED SCHOLAR PUBLICATIONS
Phytoremediation of Heavy Metal-Contaminated Sites: Eco-environmental Concerns, Field Studies, Sustainability Issues and Future Prospects G Saxena, D Purchase, SI Mulla, GD Saratale, RN Bharagava Reviews of Environmental Contamination and Toxicology 249, 71-131 , 2019 2019 Citations: 478
A comprehensive overview and recent advances on polyhydroxyalkanoates (PHA) production using various organic waste streams RG Saratale, SK Cho, GD Saratale, AA Kadam, GS Ghodake, M Kumar, ... Bioresource Technology, 124685 , 2021 2021 Citations: 348
Ecological risk assessment of pharmaceuticals in the receiving environment of pharmaceutical wastewater in Pakistan M Ashfaq, KN Khan, MSU Rehman, G Mustafa, MF Nazar, Q Sun, J Iqbal, ... Ecotoxicology and Environmental Safety 136, 31-39 , 2017 2017 Citations: 324
Strong impact of anthropogenic contamination on the co‐occurrence patterns of a riverine microbial community A Hu, F Ju, L Hou, J Li, X Yang, H Wang, SI Mulla, Q Sun, H Bürgmann, ... Environmental microbiology 19 (12), 4993-5009 , 2017 2017 Citations: 312
Applications of metagenomics in microbial bioremediation of pollutants: from genomics to environmental cleanup RN Bharagava, D Purchase, G Saxena, SI Mulla Microbial diversity in the genomic era, 459-477 , 2019 2019 Citations: 229
Characterization and identification of recalcitrant organic pollutants (ROPs) in tannery wastewater and its phytotoxicity evaluation for environmental safety RN Bharagava, G Saxena, SI Mulla, DK Patel Archives of Environmental Contamination and Toxicology 75 (2), 259-272 , 2018 2018 Citations: 215
Biodegradation of sulfamethoxazole in bacteria from three different origins SI Mulla, A Hu, Q Sun, J Li, F Suanon, M Ashfaq, CP Yu Journal of Environmental Management 206, 93-102 , 2018 2018 Citations: 212
Regeneration and reusability of non-conventional low-cost adsorbents to remove dyes from wastewaters in multiple consecutive adsorption–desorption cycles: a review N El Messaoudi, M El Khomri, A El Mouden, A Bouich, A Jada, A Lacherai, ... Biomass conversion and biorefinery 14 (11), 11739-11756 , 2024 2024 Citations: 207
Purification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization ZK Bagewadi, SI Mulla, HZ Ninnekar Journal of Genetic Engineering and Biotechnology 15 (1), 139-150 , 2017 2017 Citations: 189
Introduction to industrial wastes containing organic and inorganic pollutants and bioremediation approaches for environmental management RN Bharagava, G Saxena, SI Mulla Bioremediation of Industrial Waste for Environmental Safety: Volume I … , 2019 2019 Citations: 188
Sustainable production of biofuels from the algae-derived biomass T Mahmood, N Hussain, A Shahbaz, SI Mulla, HMN Iqbal, M Bilal Bioprocess and biosystems engineering 46 (8), 1077-1097 , 2023 2023 Citations: 185
Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation RN Bharagava, S Mani, SI Mulla, GD Saratale Ecotoxicology and environmental safety 156, 166-175 , 2018 2018 Citations: 173
Degradation mechanism and toxicity reduction of methyl orange dye by a newly isolated bacterium Pseudomonas aeruginosa MZ520730 R Kishor, D Purchase, GD Saratale, LFR Ferreira, CM Hussain, SI Mulla, ... Journal of Water Process Engineering 43, 102300 , 2021 2021 Citations: 165
VOCs-mediated hormonal signaling and crosstalk with plant growth promoting microbes S Tyagi, SI Mulla, KJ Lee, JC Chae, P Shukla Critical Reviews in Biotechnology, 20 , 2018 2018 Citations: 157
Synthesis of Gossypium hirsutum ‐derived silver nanoparticles and their antibacterial efficacy against plant pathogens GL Vanti, VB Nargund, BK N, R Vanarchi, M Kurjogi, SI Mulla, S Tubaki, ... Applied Organometallic Chemistry 33 (1), e4630 , 2019 2019 Citations: 131
Development of ultrasound aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization GD Saratale, RG Saratale, S Varjani, SK Cho, GS Ghodake, A Kadam, ... Industrial crops and products 150, 112425 , 2020 2020 Citations: 112
Characterization of triclosan metabolism in Sphingomonas sp. strain YL-JM2C SI Mulla, H Wang, Q Sun, A Hu, CP Yu Scientific Reports 6, 21965 , 2016 2016 Citations: 109
Organophosphate Pesticides: Impact on Environment, Toxicity, and Their Degradation SI Mulla, F Ameen, MP Talwar, SAMAS Eqani, RN Bharagava, G Saxena, ... 2020 Citations: 103
Biochemical and enzyme inhibitory attributes of methanolic leaf extract of Datura inoxia Mill. ZK Bagewadi, UM Muddapur, SS Madiwal, SI Mulla, A Khan Environmental Sustainability 2 (1), 75-87 , 2019 2019 Citations: 97
Optimization of laccase production and its application in delignification of biomass ZK Bagewadi, SI Mulla, HZ Ninnekar International Journal of Recycling of Organic Waste in Agriculture 6 (4 … , 2017 2017 Citations: 96
