Sahar Ragab Mohamed El hadad

EDUCATION

1997-07 - 2010-01 Immunology and Molecular biology
Cairo University.
* Professional development completed in Immunology and Molecular Biology Ph.D

1986-01 - 1991-01 Master of Science: Cell biology and Histochemistry
Medical Research Institute, Alexandria University - Alexandria, Egypt
• Professional development completed in Oral cancer Cell Biology and histochemistry project

1981-09 - 1985-07 Bachelor of Science Degree: Zoology and Chemistry
Ain Shams University - Cairo, Egypt

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Genetics and Molecular Biology, Cell Biology, Molecular Biology, Molecular Medicine

Scopus Publications

The development of a novel oral 5-Fluorouracil in-situ gelling nanosuspension to potentiate the anticancer activity against colorectal cancer cells
Samaa Abdullah, Sahar El Hadad, Alia Aldahlawi
International Journal of Pharmaceutics, 2022
In vitro optimization, characterization and anti-tumor evaluation against colorectal cancer of a novel 5-fluorouracil oral nanosuspension using soy protein, polysaccharides-protein complexation, and in-situ gel formation
Samaa Abdullah, Sahar El Hadad, Alia Aldahlawi
Journal of Drug Delivery Science and Technology, 2022
Sustaining exposure to high concentrations of bifidobacteria inhibits gene expression of Mouse's mucosal immunity
Sahar El. Hadad, Ayeshah Zakareya, Ahmed Al-Hejin, Alia Aldahlawi, Mona Alharbi
Heliyon, 2019
Publisher Correction: In vitro and in vivo evidences for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products (Scientific Reports, (2018), 8, 1, (12553), 10.1038/s41598-018-31006-3)
Khaled Elbanna, Sahar El Hadad, Abdelrahaman Assaeedi, Alia Aldahlawi, Manal Khider, et al.
Scientific Reports, 2019
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Lactobacillus rhamnosus enhances the immunological antitumor effect of 5-fluorouracil against colon cancer
Aji Winanta, Triana Hertiani, Purwantiningsih ., Siswadi .
Pakistan Journal of Biological Sciences, 2019
BACKGROUND AND OBJECTIVE Faloak (Sterculia quadrifida R.Br) is widely used as traditional medicine in Indonesia to improve stamina (reduce tiredness for heavy workers). However, no scientific reports so far on the immunomodulatory effect. The aim of this study was to determine the effect of the bark of faloak as immunomodulatory agents by evaluating their effect on BALB/c mice lymphocytes proliferation, the activity of macrophage, nitric oxide production and the immunoglobulin G titer by in vivo techniques. MATERIALS AND METHODS Decoction of the faloak bark was used for the in vivo assay. BALB/c mice were divided into 5 dose groups, each consisting of 5 mice. One group was chosen as the baseline, 3 groups were used for the group treated with the test substance at doses of 7.5, 11.75 and 17.5 g kg-1 of body weight of mice (p.o) and a positive control group was treated with Phyllanthus niruri Linn. (PN) extract (Stimuno®) 0.585 g kg-1 b.wt., (p.o). The test samples were given every day. All mice were induced by hepatitis B vaccine at day 7 and 14. The activity of in vivo assay was determined at day 19. The activity of immunomodulatory effect is expressed in phagocytic capacity, phagocytosis index, nitric oxide, OD of lymphocyte proliferation and IgG titers. RESULTS The macrophage phagocytic capacity and phagocytosis index were significantly increased (p<0.05), nitric oxide production were altered significantly (p<0.05), but OD of lymphocyte proliferation and production of IgG titers were unchanged (p>0.05). CONCLUSION This study showed that the faloak bark could increase the macrophages phagocytic activity, but no effect on lymphocyte cells and therefore did not influence the adaptive immune response.
In vitro and in vivo evidences for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products
Khaled Elbanna, Sahar El Hadad, Abdelrahaman Assaeedi, Alia Aldahlawi, Manal Khider, et al.
Scientific Reports, 2018
Probiotics are commensals with special characteristics that are essential for the development of the immune system, and may protect mucosal surfaces against pathogens. In this study, a total of 40 lactic acid bacteria (LAB) were isolated from different raw and fermented camel’s milk samples collected from Saudi Arabia (Makkah area) and Egypt (Fayoum), and tested for the probiotic properties. Among them, Pro 4 and Pro 7 isolates exhibited excellent probiotic potential including bile salt (0.2–0.6%), phenol tolerance (0.2–0.4%) and salt tolerance (0.0–10%). Furthermore, both strains exhibited antimicrobial activity against wide range of food-borne pathogens and Dermatophytes with average zone inhibition of 37.5, 35.5, 34.5, 27.5, 25 and 23.5 mm for Staphylococcus aureus, Trichophyton mentagrophytes, Escherichia coli, Listeria monocytogens, Candida albicans and Salmonella typhi, respectively. Furthermore, the in vivo study indicated that these strains significantly improved the mucosal immune responses through an increase in expression of TLR2 and IFNγ mRNA in mice intestine as well as increased the synthesis of polyclonal IgG, IgM and IgA in mice blood sera. Accordingly, due to these unique probiotic properties, both selected strains could be potentially used as probiotic starter cultures for fermented dairy foods as well as functional food and health products.
Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia
Sahar EL Hadad, Saleha Alakilli, Samar Rabah, Jamal Sabir
Saudi Journal of Biological Sciences, 2018
Molecular characterization of dengue E/NS1 junction genotype 2 isolated from Saudi patients, Jeddah province
Sahar EL Hadad, Alawiah Alhebshi, Haifa Al Amri
Pakistan Journal of Biological Sciences, 2018
BACKGROUND AND OBJECTIVE Dengue is the most important arthropod-borne viral disease which caused by the four-dengue virus serotypes (1-4) and its incidence has grown dramatically around the world in the recent decades. This study was conducted to determine the molecular characterization of dengue virus genotypes spreading in Jeddah province. METHODOLOGY To distinguish dengue virus genotypes, serum samples from 13 infected patients were subjected to envelop and non-structural 1 (E/NS1) gene amplification and sequence analysis at the nucleotide and amino acid levels. RESULTS The present partial dengue virus phylogenetic analysis announced the domination of dengue virus 2 genotype among the current dengue virus samples circulating in Jeddah province. Dengue virus 2 current isolates were grouped in one branch and seemed to be more closely related to various strains isolated from Sri-Lanka, Australia and Singapore and confirmed by internucleotide distance average ranged +/-0.01. Interestingly, sequences analysis of amino acids confirmed substitution of 8 amino acid residue (Ser729Gua, Ser729Arg, Val762Gau, Val780phe, Val781Leu, Val781Ala, Glu858Asp and Gln873His) among the present isolates comparing with previous references strains isolated from different countries. Remarkably, one unique amino acid residue Ala741Val was verified in the 10 present isolates compared to the reference sequence previously isolated from Jeddah. CONCLUSION Notably, the present study demonstrated the sequencing analysis of the dengue virus 2-E/NS1 on both nucleotide and amino acid levels and confirmed its endogenously prevalence in Jeddah.
Partial sequencing analysis of the NS5B region confirmed the predominance of hepatitis C virus genotype 1 infection in Jeddah, Saudi Arabia
Sahar EL Hadad, Hesa Al-Hamdan, Sabah Linjawi
Plos One, 2017
Chronic hepatitis C virus (HCV) infection and its progression are major health problems that many countries including Saudi Arabia are facing. Determination of HCV genotypes and subgenotypes is critical for epidemiological and clinical analysis and aids in the determination of the ideal treatment strategy that needs to be followed and the expected therapy response. Although HCV infection has been identified as the second most predominant type of hepatitis in Saudi Arabia, little is known about the molecular epidemiology and genetic variability of HCV circulating in the Jeddah province of Saudi Arabia. The aim of this study was to determine the dominance of various HCV genotypes and subgenotypes circulating in Jeddah using partial sequencing of the NS5B region. To the best of our knowledge, this is the first study of its kind in Saudi Arabia. To characterize HCV genotypes and subgenotypes, serum samples from 56 patients with chronic HCV infection were collected and subjected to partial NS5B gene amplification and sequence analysis. Phylogenetic analysis of the NS5B partial sequences revealed that HCV/1 was the predominant genotype (73%), followed by HCV/4 (24.49%) and HCV/3 (2.04%). Moreover, pairwise analysis also confirmed these results based on the average specific nucleotide distance identity: ±0.112, ±0.112, and ±0.179 for HCV/1, HCV/4, and HCV/3, respectively, without any interference between genotypes. Notably, the phylogenetic tree of the HCV/1 subgenotypes revealed that all the isolates (100%) from the present study belonged to the HCV/1a subgenotype. Our findings also revealed similarities in the nucleotide sequences between HCV circulating in Saudi Arabia and those circulating in countries such as Morocco, Egypt, Canada, India, Pakistan, and France. These results indicated that determination of HCV genotypes and subgenotypes based on partial sequence analysis of the NS5B region is accurate and reliable for HCV subtype determination.
Identification of novel D11 hepatitis B surface antigen subgenotype in Jeddah, Kingdom of Saudi Arabia
Sahar El Hadad, Saleha Alakilli, Samar Rabah, Jamal Sabir
Biotechnology, 2015
Hepatitis B virus (HBV) infection remains to be a worldwide health problem. In Saudi Arabia, HBV is the most predominant type of hepatitis followed by hepatitis C and hepatitis A while little is known about the molecular epidemiology of the prevalence of HBV genotype/subgenotype particularly in Jeddah. Serum samples were collected from HBV chronic patients and subjected to HBsAg gene amplification. Sequencing and phylogenetic analysis of the entire HBsAg gene sequences revealed that 11 isolates belonged to HBV/D while 4 isolates were associated with HBV/C. Interestingly, HBV/D subgenotypes identified eight HBV/D present isolates belonged to HBV/D1 while three isolates showed a new cluster supporting by a branch with 99% bootstrap value and 4.3-5.8% nucleotide divergence over the entire HBsAg gene from other known subgenotypes D1 to D10, despite they were appearing more related to HBV/D5. The three strains of the new D subgenotype showed unique amino acid sequences consisting of Thr7non, 75Pro in the preS1 gene, 112Ile, 161Gly in the preS2 gene and 196Glu, 197Ala, 238Ser, 259Cys in the S gene. In addition to three amino acid residues in the S gene (373Ile, 374Ala and 381Thr) were specified S118S isolate. Subsequently, it have been verified that HBV/D1 is the most prevalent HBV subgenotype in Jeddah as well as we proposed a novel subgenotype designated HBV/D11. The identification of HBV/D11 novel subgenotypes in the present study suggested that further studies with a large number of subjects in previously examined and unexamined areas may lead to discovering new HBV strain genotypes and/or subgenotypes circulating in Saudi Arabia.
Histological changes of Mice lungs after daily exposure to different concentration of Incense smoke
Life Science Journal, 2013
Ultrastructural changes occur in mice lungs after cessation to exposure of incense smoke
Life Science Journal, 2012

Publications

EL Hadad S, Alhebshi A and Al Amri A, 2018. Molecular Characterization of Dengue E/NS1 Junction Genotype 2 Isolated from Saudi Patients, Jeddah Province. Pakistan Journal of Biological Sciences, 21: 38-50.
Elbanna, K, El Hadad S, Assaeedi A, Aldahlawi A, Khider A& Alhebshi A. (2018) In vitro and in vivo evidence for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products. Scientific Reports 8:12553 DOI:10.1038/s41598-018-31006-3
EL Hadad S, Alhebshi A and Al Amri A, 2018. Molecular Characterization of Dengue E/NS1 Junction Genotype 2 Isolated from Saudi Patients, Jeddah Province. Pakistan Journal of Biological Sciences, 21: 38-50.
El Hadad S, Zakareya A, Hejin A, Aldahlawi A and Al Harbi M. 2019 A Sustaining Exposure to High Concentrations of Bifidobacteria Inhibit Gene Expression of Mice's Mucosal immunity. Heliyon. 12;5(12):e02866. doi: 10.1016/j..
El Hadad S, Al Hazmi B, Alhebshi A, Aldahlawi AM, and Al Bassam R. 2019 Lactobacillus rhamnosus Enhances the Immunological Antitumor Effect of 5' Fluorouracil against Colon Cancer. Pakistan Journal of Biological Sciences, 22:597-606.
Abdullah S, El Hadad S, Aldahlawi A (2021). The development of a novel oral 5-Fluorouracil in-situ gelling nanosuspension to potentiate the anticancer activity against colorectal cancer cells. Int J Pharm. 2022 Feb 5;613:121406. doi: 10.1016/j.. Epub 2021 Dec 27

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

1- Patent number: 11285104,
Title: ORAL ADMINISTRATION OF 5-FU IN A GELLING NANOSUSPENSION FOR TARGETED DELIVERY TO TREAT COLORECTAL CANCERS
Type: Grant
Filed: May 21, 2021
Date of Patent: March 29, 2022
Assignee: KING ABDULAZIZ UNIVERSITY
Inventors: Alia M Aldahlawi, Sahar R El Hadad, Samaa T Abdullah
2- Patent number: 11058635
Title: ORAL ADMINISTRATION OF 5-FU IN A GELLING NANOSUSPENSION FOR TARGETED DELIVERY TO TREAT COLORECTAL CANCERS
Type: Grant
Filed: October 15, 2020
Date of Patent: July 13, 2021
Assignee: King Abdulaziz University
Inventors: Alia M Aldahlawi, Sahar R El Hadad, Samaa T Abdullah