Sudipta Chakrabarti
@mcconline.org.in
Principal & Associate Professor of Biological Sciences
Midnapore City College
Neuro biology
RESEARCH, TEACHING, or OTHER INTERESTS
Molecular Medicine, Immunology, Neuroscience, Cellular and Molecular Neuroscience
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Tridip Kumar Das, Priyanka Kar, Titli Panchali, Amina Khatun, Ananya Dutta, Smita Ghosh, Sudipta Chakrabarti, Shrabani Pradhan, Keshab Chandra Mondal, and Kuntal Ghosh
Springer Science and Business Media LLC
Sudipta Chakrabarti, Sujyoti Chandra, Avik Roy, Madhuchhanda Kundu, and Kalipada Pahan
Elsevier BV
Pipika Das, Ananya Dutta, Titli Panchali, Amina Khatun, Riya Kar, Tridip Kumar Das, Manisha Phoujdar, Sudipta Chakrabarti, Kuntal Ghosh, and Shrabani Pradhan
Elsevier BV
Titli Panchali, Ananya Dutta, Pipika Das, Amina Khatun, Riya Kar, Subhadeep Mondal, Keshab Chandra Mondal, Sudipta Chakrabarti, Kuntal Ghosh, and Shrabani Pradhan
University of South Bohemia in Ceske Budejovice
We have extracted and characterized Phasa fish (Setipinna phasa) oil for the first time to evaluate the anti-obesity and related anti-inflammatory effects on obese mice. Inbred male albino BALB/c mice were segregated into three categories: control (C), Obese control group (OC), and Phasa fish oil treated group (TX). To establish the potentiality of Setipinna phasa oil for its anti-obesity and anti-inflammatory properties, it was extracted and characterized using GC-MS method. To evaluate the anti-obesity effect, different parameters were considered, such as body weight, lipid composition, obesity, and obesity associated inflammation. The physicochemical characteristics of Phasa fish oil revealed that the oil quality was good because acid value, peroxide value, p-anisidine value, Totox value, refractive index, and saponification value were within the standard value range. The GC-MS study explored the presence of fatty acids beneficial to health such as Hexadec-9-enoic acid; Octadec-11-enoic acid; EPA, DHA, Methyl Linolenate, etc. The application of Setipinna phasa oil on the treated mice group acutely lowered body weight and serum lipid profile compared to the obese group. In connection with this, leptin, FAS, and pro-inflammatory cytokines TNF-α genes expression were downregulated in the treated group compared to the obese group. The Phasa oil treated group had an elevated expression of PPAR-α, adiponectin, LPL gene, and anti-inflammatory markers IL-10 and IL-1Ra compared to the obese group. This study suggests that Phasa fish oil, enriched with essential fatty acid, might be used as an anti-obesity and anti-inflammatory supplement.
Amina Khatun, Titli Panchali, Sukhamoy Gorai, Ananya Dutta, Tridip Kumar Das, Kuntal Ghosh, Shrabani Pradhan, Keshab Chandra Mondal, and Sudipta Chakrabarti
Informa UK Limited
OBJECTIVES
In this study mice were fed a high-fat diet for 12 weeks to establish diet-induced obesity and syringic acid (SA) was assessed for anti-obese, neuroprotective, and neurogenesis.
METHOD
Animals were given HFD for 12 weeks to measure metabolic characteristics and then put through the Barns-maze and T-maze tests to measure memory. Additionally, the physiology of the blood-brain barrier, oxidative stress parameters, the expression of inflammatory genes, neurogenesis, and histopathology was evaluated in the brain.
RESULT
DIO raised body weight, BMI, and other metabolic parameters after 12 weeks of overfeeding. A reduced spontaneous alternation in behavior (working memory, reference memory, and total time to complete a task), decreased enzymatic and non-enzymatic antioxidants, oxidative biomarkers, increased neurogenesis, and impaired blood-brain barrier were all seen in DIO mice. SA (50 mg/kg) treatment of DIO mice (4 weeks after 8 weeks of HFD feeding) reduced diet-induced changes in lipid parameters associated with obesity, hepatological parameters, memory, blood-brain barrier, oxidative stress, neuroinflammation, and neurogenesis. SA also reduced the impact of malondialdehyde and enhanced the effects of antioxidants such as glutathione, superoxide dismutase (SOD), and total thiol (MDA). Syringic acid improved neurogenesis, cognition, and the blood-brain barrier while reducing neurodegeneration in the hippocampal area.
DISCUSSION
According to the results of the study, syringic acid therapy prevented neurodegeneration, oxidative stress, DIO, and memory loss. Syringic acid administration may be a useful treatment for obesity, memory loss, and neurogenesis, but more research and clinical testing is needed.
Ananya Dutta, Titli Panchali, Amina Khatun, Sreenivasa Rao Jarapala, Koushik Das, Kuntal Ghosh, Sudipta Chakrabarti, and Shrabani Pradhan
Springer Science and Business Media LLC
AbstractThe implication of inflammation in the pathophysiology of several types of cancers has been under intense investigation. Conjugated fatty acids can modulate inflammation and present anticancer effects, promoting cancer cell death. In this paper, we evaluated the efficacy of new conjugated fatty acids isolated from marine Opisthopterus tardoore (Tapra fish) in human breast cancer cell lines MCF-7. Linoelaidic acid, a marine fish (O. tardoore) derived unsaturated fatty acids, showed effective anticancer activity against MCF-7. Cell viability (MTT) assay revealed a dose-dependent decline in cancer cell viability. It was noteworthy that 5 µM linoelaidic acid decreased the MCF-7 cell viability by 81.82%. Besides that, linoelaidic acid significantly (P< 0.05) increased the level of tumor necrosis factor-α (TNF-α) and interleukin-1 receptor antagonist (IL-1ra) studied by ELISA. Not only that, linoelaidic acid significantly decreased the reduced glutathione level and increased the oxidized glutathione level in MCF-7 cells indicating the oxidative stress inside the cell. Two different cell staining methods with acridine orange-ethidium bromide and DAPI confirmed that the linoelaidic acid rendered their detrimental effect on cancer cells. To decipher the mode of apoptosis Western blotting was performed in which the expression pattern of several proteins (p53, IL-10, and IL-1ra) established the apoptosis in the studied cell lines after linoelaidic acid exposure. Hence it may be conferred that linoelaidic acid has prompt anticancer activity. Therefore this drug can be used further for the treatment of cancer.
Smita Ghosh, Priyanka Kar, Sudipta Chakrabarti, Shrabani Pradhan, Keshab Chandra Mondal, and Kuntal Ghosh
Elsevier BV
Smita Ghosh, Priyanka Kar, Sudipta Chakrabarti, Shrabani Pradhan, Keshab Chandra Mondal, and Kuntal Ghosh
Springer Science and Business Media LLC
Surendra Patra, Sukhamoy Gorai, Soumitra Pal, Kuntal Ghosh, Shrabani Pradhan, and Sudipta Chakrabarti
Wiley
Phytoestrogens are plant secondary metabolite that is structurally and functionally similar to mammalian estrogens, which have been shown to have various health benefits in humans. Isoflavones, coumestans, and lignans are the three major bioactive classes of phytoestrogens. It has a complicated mechanism of action involving an interaction with the nuclear estrogen receptor isoforms ERα and ERβ, with estrogen agonist and estrogen antagonist effects. Depending on their concentration and bioavailability in various plant sources, phytoestrogens can act as estrogen agonist or antagonists. Menopausal vasomotor symptoms, breast cancer, cardiovascular disease, prostate cancer, menopausal symptoms, and osteoporosis/bone health have all been studied using phytoestrogens as an additional standard hormone supplemental remedy. The botanical sources, techniques of identification, classification, side effects, clinical implications, pharmacological and therapeutic effects of their proposed mode of action, safety issues, and future directions for phytoestrogens have all been highlighted in this review.
Priyanka Kar, Smita Ghosh, Pijush Payra, Sudipta Chakrabarti, Shrabani Pradhan, Keshab Ch. Mondal, and Kuntal Ghosh
Springer Science and Business Media LLC
Koustav Chatterjee, Sankar Deb Roy, Koushik Chakraborty, Asmaul Haque, Sudipta Chakrabarti, Syamantak Mukherjee, Sudipa Mal, Nilanjana Das, Sushil Kumar Sahu, Nabanita Roy Chattopadhyay,et al.
Springer Science and Business Media LLC
Tridip K. Das, Shrabani Pradhan, Sudipta Chakrabarti, Keshab Chandra Mondal, and Kuntal Ghosh
Elsevier BV
Priyanka Kar, Tridip Kr. Das, Smita Ghosh, Shrabani Pradhan, Sudipta Chakrabarti, Keshab Ch. Mondal, and Kuntal Ghosh
Elsevier BV
Papan Kumar Hor, Shilpee Pal, Joy Mondal, Suman Kumar Halder, Kuntal Ghosh, Sourav Santra, Mousumi Ray, Debabrata Goswami, Sudipta Chakrabarti, Somnath Singh,et al.
Frontiers Media SA
The present study has been aimed at evaluating the antiobesity, antihyperglycemic, and antidepressive potentials of Asparagus racemosus starter-based rice fermented foods. High-throughput NGS technology has revealed a number of bacterial genera in the prepared fermented rice, such as Lactobacillus (29.44%), Brevundimonas (16.21%), Stenotrophomonas (6.18%), Pseudomonas (3.11%), Bacillus (2.88%), and others (&lt;2%). Eight-week administration of rice fermented food has increased food intake, whole-body weight, organ weight, different fat masses, serum lipid profiles, and histology of liver and adipose tissues in HFD-induced obese mice. In addition, upregulation of fatty acid oxidation and downregulation of adipocytogenesis- and lypogenesis-related genes along with the expression of their regulatory nuclear factors such as PPARα, PPARγ, PPARδ, and SREBP-1c have also been noted. Moreover, fermented food decreases fasting blood glucose level and improves glucose and insulin tolerance as well as the expression of GLUT4 receptor. Antiobesity and antihyperglycemic effects are also supported by the changes in insulin, leptin, and adiponectin hormone levels. The real-time polymerase chain reaction (RT-PCR) and denaturing gradient gel electrophoresis (DGGE) analyses have clearly demonstrated the intense colonization of Bacteroides, Lactobacillus, and Bifidobacterium, as well as the suppressed growth rate of γ- and δ-Proteobacteria and Firmicutes in the gut after fermented food intake. In the intestine, the latter group of microorganisms possibly modulate short-chain fatty acid (SCFA) levels such as acetate, butyrate, and propionate more than twofold. The impairment of memory-learning and anxiety-like obesity-associated cognitive phenotypes is mitigated significantly (p &lt; 0.01) by fermented food as well. Thus, the formulated fermented food could be used as a natural therapeutic to alleviate obesity and its associated psychological and pathophysiological ailments.
Avik Roy, Madhuchhanda Kundu, Sudipta Chakrabarti, Dhruv R. Patel, and Kalipada Pahan
IOS Press
Background: Doublecortin (DCX), a microtubule associated protein, has emerged as a central biomarker of hippocampal neurogenesis. However, molecular mechanisms by which DCX is regulated are poorly understood. Objective: Since sleep is involved with the acquisition of memory and oleamide or 9-Octadecenamide (OCT) is a sleep-inducing supplement in human, we examined whether OCT could upregulate DCX in hippocampal progenitor cells (HPCs). Methods: We employed real-time PCR, western blot, immunostaining, chromatin immunoprecipitation, lentiviral transduction in HPCs, and the calcium influx assay. Results: OCT directly upregulated the transcription of Dcx in HPCs via activation of peroxisome proliferator-activated receptor α (PPARα), a lipid-lowering transcription factor. We observed that, HPCs of Ppara-null mice displayed significant impairment in DCX expression and neuronal differentiation as compared to that of wild-type mice. Interestingly, treatment with OCT stimulated the differentiation process of HPCs in wild-type, but not Ppara-null mice. Reconstruction of PPARα in mouse Ppara-null HPCs restored the expression of DCX, which was further stimulated with OCT treatment. In contrast, a dominant-negative mutant of PPARα significantly attenuated the stimulatory effect of OCT on DCX expression and suppressed neuronal differentiation of human neural progenitor cells. Furthermore, RNA microarray, STRING, chromatin immunoprecipitation, site-directed mutagenesis, and promoter reporter assay have identified DCX as a new target of PPARα. Conclusion: These results indicate that OCT, a sleep supplement, directly controls the expression of DCX and suggest that OCT may be repurposed for stimulating the hippocampal neurogenesis.
Sudipta Chakrabarti, Tim Prorok, Avik Roy, Dhruv Patel, Sridevi Dasarathi, and Kalipada Pahan
IOS Press
Background: Neuroinflammation is a recognized aspect of Alzheimer’s disease (AD) and other neurological illnesses. Interleukin 1 receptor antagonist (IL-1Ra) is an anti-inflammatory molecule, which inhibits inflammatory molecules in different cells including brain cells. However, mechanisms for upregulating IL-1Ra in brain cells are poorly understood. Objective: Since aspirin is a widely available pain reliever that shows promise beyond its known pain-relieving capacity, we examined whether aspirin could upregulate the IL-1Ra in the brain. Methods: We employed PCR, real-time PCR, western blot, immunostaining, chromatin immunoprecipitation (ChIP), and lentiviral transduction in glial cells. 5xFAD mice, an animal model of AD, were treated with aspirin orally via gavage. Results: Aspirin increased the expression of IL-1Ra mRNA and protein in primary mouse astrocytes and mouse BV-2 microglial cells. While investigating the mechanism, we found that the IL-1Ra gene promoter harbors peroxisome proliferator response element (PPRE) and that aspirin upregulated IL-1Ra in astrocytes isolated from peroxisome proliferator-activated receptor-beta knockout (PPARβ–/–), but not PPARα–/–, mice. Moreover, we observed that aspirin bound to tyrosine 314 residue of PPARα to stimulate IL-1Ra and that aspirin treatment also increased the recruitment of PPARα to the IL-1Ra promoter. Accordingly, aspirin increased IL-1Ra in vivo in the brain of wild type and PPARβ–/–, but not in PPARα–/– mice. Similarly, aspirin treatment also increased astroglial and microglial IL-1Ra in the cortex of 5xFAD, but not 5xFAD/PPARα–/– mice. Conclusion: Aspirin may reduce the severity of different neurological conditions by upregulating IL-1Ra and reducing the inflammation.
Shrabani Pradhan, Titli Panchali, Bani Paul, Amina Khatun, Sreenivasa Rao Jarapala, Keshab Chandra Mondal, Kuntal Ghosh, and Sudipta Chakrabarti
Hindawi Limited
In this present investigation, we have extracted and characterized the Tapra fish oil as well as applied it to evaluate anti-obesity potentiality. The Tapra fish oil had 1.14 ± 0.10 mg KOH/g of acid value, 129.8 ± 5.09 mg KOH/g of saponification number, 2.67 ± 0.67 mEq/kg of peroxide value, 121.9 ± 2.14 mg of iodine value, and 17.67 ± 1.45 totox value. Gas Chromatography-Mass Spectrometric analysis clearly revealed the presence of nine different fatty acids. When the fish oil was applied to high-fat diet-induced obese mice, it showed significant reduction of body weight, Body Mass Index, and serum lipid profiles compared to the high-fat diet-induced obese mice. The levels of leptin and TNF-α were moderately reduced in fish oil treated high-fat diet-induced obese mice than control obese mice. In conclusion, the Tapra fish oil was enriched with essential fatty acids and it could be used as an antiobese food supplement. PRACTICAL APPLICATIONS: Considering the adverse effects of drugs used for the treatment of obesity, there is always a need to find out the alternatives. While the anti-obesity potentialities of different sea fish oil have been documented, the same for the Tapra fish (Opisthopterus tardoore) oil has not been studied at all. The extracted Tapra fish oil was found good in quality. Administration of fish oil in the mice exhibited anti-obesity effect in terms of lowering body weight, Body Mass Index, and serum lipid profiles, leptin, and TNF-α in mice model. These findings are fostering new therapeutic approaches to obesity treatment.
Nabanita Roy Chattopadhyay, Koustav Chatterjee, Nikhil Tiwari, Sudipta Chakrabarti, Sushil Kumar Sahu, Sankar Deb Roy, Arijit Ghosh, R. Rajendra Reddy, Piyanki Das, Sudipa Mal,et al.
Elsevier BV
Sudipta Chakrabarti, Avik Roy, Tim Prorok, Dhruv Patel, Sridevi Dasarathi, and Kalipada Pahan
Wiley
Neuroinflammation is being recognized as a hallmark of different neurodegenerative disorders, including Alzheimer’s disease. Suppressor of cytokine signaling 3 (SOCS3) is an anti‐inflammatory molecule, which is known to inhibit cytokine signaling and inflammatory gene expression in different cells. However, the pathways by which SOCS3 could be up‐regulated in brain cells are poorly understood. Aspirin is a widely available pain reliever that is showing promise beyond its known pain‐relieving capacity. This study underlines the importance of aspirin in upregulating SOCS3 in astrocytes and microglia. Aspirin increased the expression of Socs3 mRNA and protein in mouse astrocytes and BV‐2 microglial cells in both a time‐ and dose‐dependent manner. While investigating the mechanism, we found that Socs3 gene promoter harbors peroxisome proliferator response element and that aspirin up‐regulated SOCS3 in astrocytes isolated from PPARβ (−/−), but not PPARα (−/−), mice. Accordingly, aspirin increased SOCS3 in vivo in the cortex of wild type and PPARβ (−/−), but not PPARα (−/−), mice. Similarly, aspirin treatment increased astroglial and microglial SOCS3 in the cortex of FAD5X, but not FAD5X/PPARα (−/−), mice. Finally, recruitment of PPARα by aspirin to the proximal, but not distal, peroxisome proliferator response element of the Socs3 promoter suggests that aspirin increases the transcription of Socs3 gene via PPARα. This study describes a novel property of aspirin in elevating SOCS3 in glial cells via PPARα and suggests that aspirin may be further considered for therapeutic application in neuroinflammatory and neurodegenerative disorders.
Nabanita Roy Chattopadhyay, Sudipta Chakrabarti, Koustav Chatterjee, Sankar Deb Roy, Sushil Kumar Sahu, R. Rajendra Reddy, Piyanki Das, Basab Bijay Kanrar, Ashok Kumar Das, Sam Tsering,et al.
Wiley
Nasopharyngeal carcinoma (NPC) is one the most confusing and rare malignancy in most part of the world with significantly high occurrence in some populations of Southeast Asia, North Africa and Alaska. Apart from the dietary and environmental factors, NPC is well‐associated with Epstein‐Barr virus (EBV) infection in these ethnic groups. However, the internal molecular mechanism(s) for such association in specific populations is not known till date. Polymorphisms in the genes of histocompatibility locus antigens (HLA) are reported in NPC, but association of any particular polymorphism with ethnicity is not established yet. Here, we report a set of HLA polymorphisms in EBV‐infected NPC samples from Northeast Indian population. These polymorphisms might play an important role for the lack of proper immune function against EBV infection and thus, eventually, for NPC generation in endemic populations like those of Northeast India.
Sudipta Chakrabarti, Sujyoti Chandra, Avik Roy, Sridevi Dasarathi, Madhuchhanda Kundu, and Kalipada Pahan
Elsevier BV
Sudipta Chakrabarti, Malabendu Jana, Avik Roy, and Kalipada Pahan
Bentham Science Publishers Ltd.
Background: Neuroinflammation plays an important role in the pathogenesis of various neurodegenerative diseases including Alzheimer’s disease (AD). Suppressor of cytokine signaling 3 (SOCS3) is an anti-inflammatory molecule that suppresses cytokine signaling and inflammatory gene expression in different cells including microglia. Objective: The pathways through which SOCS3 could be upregulated are poorly described. Cinnamic acid is a metabolite of cinnamon, a natural compound that is being widely used all over the world as a spice or flavoring agent. Here, we examined if cinnamic acid could upregulate SOCS3 in microglia. Method: Microglia and astroglia isolated from mouse brain as well as BV-2 microglial cells were treated with cinnamic acid followed by monitoring the level of SOCS3 and different proinflammatory molecules by RT-PCR and real-time PCR. To nail down the mechanism, we also performed ChIP analysis to monitore the recruitment of cAMP response element binding (CREB) to the socs3 gene promoter and carried out siRNA knockdown of CREB. Results: Cinnamic acid upregulated the expression of SOCS3 mRNA and protein in mouse BV-2 microglial cells in dose- and time-dependent manner. Accordingly, cinnamic acid also increased the level of SOCS3 and suppressed the expression of inducible nitric oxide synthase and proinflammatory cytokines (TNFα, IL-1β and IL-6) in LPSstimulated BV-2 microglial cells. Similar to BV-2 microglial cells, cinnamic acid also increased the expression of SOCS3 in primary mouse microglia and astrocytes. We have seen that cAMP response element is present in the promoter of socs3 gene, that cinnamic acid induces the activation of CREB, that siRNA knockdown of CREB abrogates cinnamic acid-mediated upregulation of SOCS3, and that cinnamic acid treatment leads to the recruitment of CREB to the socs3 gene. Conclusions: These studies suggest that cinnamic acid upregulates the expression of SOCS3 in glial cells via CREB pathway, which may be of importance in neuroinflammatory and neurodegenerative disorders.
Manu Asthana, Sushil Kumar Sahu, Amit Kumar, Suchitra Mohanty, Sudipta Chakrabarti, Piyanki Das, Nabanita Roy Chattopadhya, Koustav Chatterjee, Shivaram Prasad Singh, Shanmugam Rajasubramaniam,et al.
Bentham Science Publishers Ltd.
BACKGROUND
Interleukin-28B (IL28B) locus on a human chromosomal region mapped to 19q13 execute immune defense against viruses. During Hepatitis C Virus (HCV) infection the IL28B has a promising role in deciding the consequence of infection for spontaneous clearance of viruses or causing chronic liver infection. Treatment of chronic hepatitis C includes use of direct acting antivirals, Pegylated-Interferon (PEG-IFN) and Ribavirin (RBV) therapy. Also, interferon free regimens are suggested to be useful in resistant patients. Numerous reports including Genome-Wide Association Studies (GWAS), comprehensive meta-analysis and independent case-control studies in different population have revealed the association between certain Il-28B polymorphisms and response to the PEGIFN- RBV therapy in patients infected with HCV.
METHOD
We searched all peer-reviewed relevant and recent literature manually for the present review.
CONCLUSION
The GWAS studies have revealed an important role of IL28B in HCV infection, which was supported by many independent studies and meta-analysis by different groups in different ethnicities. IL28B genotyping may be use as predictors of response for IFN-based therapy and personalized treatment of hepatitis C patient.