Dr Shanmuga Sundar Saravanabhavan
@avit.ac.in
Assistant Professor (Grade - II), Department of Biotechnology, Faculty of Technology
Aarupadai Veedu Institute of Technology
EDUCATION
B. Tech, MS (UK), MS (Res) in Tech, PhD
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Molecular Medicine, Cancer Research, Biomedical Engineering
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Suthinee Sangkana, Komgrit Eawsakul, Tassanee Ongtanasup, Rachasak Boonhok, Watcharapong Mitsuwan, Siriphorn Chimplee, Alok K. Paul, Shanmuga Sundar Saravanabhavan, Tooba Mahboob, Muhammad Nawaz,et al.
Royal Society of Chemistry (RSC)
Garcinia mangostana extract (GME) has severe pharmacokinetic deficiencies and is made up of a variety of bioactive components.
Sundar S. Shanmuga, Kannan Natarajan, Sarang Zsolt, Lakshminarayanan Srimathi Priya, Manikandan Ayyar, Sasikala Anboo, Veeranoot Nissapatorn, Narendranath Jonna, Kamalakannan Vasanthapalaniappan, Rajavelu Packirisamy,et al.
Walter de Gruyter GmbH
Abstract There have been significant developments in the use of siRNA in the silencing of cancer-allied target proteins with substantial apoptotic effects. Nevertheless, the challenges regarding siRNA delivery for cancer therapy remain a major concern for taking these therapies successfully from laboratory to in vivo studies. Biomaterials are defined traditionally as any material that is used for either medical or dental applications that contact the host cells in any form, such as a drug carrier a device, or as a prosthesis towards the replacement of damaged tissues. The main issues to be fulfilled by the biomaterial for clinical applications are biocompatibility, bioactivity, ability to carry drug to target site, inflammatory responses and other factors based on its application. The present study focuses on the in vitro inflammatory response to the CS nanoparticles using RAW 264.7 and bone marrow derived macrophage cells. Additionally, the in vitro release kinetics of siRNA with varying concentrations and pH, transfection efficacy and biocompatibility were also investigated. The results of siRNA cumulative release increased at pH 5 and 3, which may be corresponding to the protonation, and a delayed release was seen at 7, which was ascribed to unprotonated amine groups inside the CS. The results of release kinetics confirmed a sustained release of siRNA from CS NPs. Considering that CS is a biocompatible polymer, it typically has little impact/damage on cells, as numerous researchers have observed during in vitro experiments. Inflammatory studies were carried out in vitro with RAW 264.7 and BMC cells derived from mice. The gene and protein expression studies showed that the materials might cause some slight inflammation on exposure with both RAW 264.7 and BMC cells in vitro, which is completely negligible. However, putting together the overall data it can be concluded that CS NPs can be a promising material for in vivo applications, which is in agreement with the results of other researchers, but the only concern being its ability to carry siRNA and protect it from nuclease and other enzymatic attacks.
Deepan Gautam, Karma Gurmey Dolma, Bidita Khandelwal, Madhu Gupta, Meghna Singh, Tooba Mahboob, Anil Teotia, Prasad Thota, Jaydeep Bhattacharya, Ramesh Goyal,et al.
PeerJ
The biosynthesis of nanoparticles using the green route is an effective strategy in nanotechnology that provides a cost-effective and environmentally friendly alternative to physical and chemical methods. This study aims to prepare an aqueous extract of Ocimum sanctum (O. sanctum)-based silver nanoparticles (AgNPs) through the green route and test their antibacterial activity. The biosynthesized silver nanoparticles were characterised by colour change, UV spectrometric analysis, FTIR, and particle shape and size morphology by SEM and TEM images. The nanoparticles are almost spherical to oval or rod-shaped with smooth surfaces and have a mean particle size in the range of 55 nm with a zeta potential of −2.7 mV. The antibacterial activities of AgNPs evaluated against clinically isolated multidrug-resistant Acinetobacter baumannii (A. baumannii) showed that the AgNPs from O. sanctum are effective in inhibiting A. baumannii growth with a zone of inhibition of 15 mm in the agar well diffusion method and MIC and MBC of 32 µg/mL and 64 µg/mL, respectively. The SEM images of A. baumannii treated with AgNPs revealed damage and rupture in bacterial cells. The time-killing assay by spectrophotometry revealed the time- and dose-dependent killing action of AgNPs against A. baumannii, and the assay at various concentrations and time intervals indicated a statistically significant result in comparison with the positive control colistin at 2 µg/mL (P < 0.05). The cytotoxicity test using the MTT assay protocol showed that prepared nanoparticles of O. sanctum are less toxic against human cell A549. This study opens up a ray of hope to explore the further research in this area and to improve the antimicrobial activities against multidrug resistant bacteria.
Nithya Ramasamy, Anbudayanidhi Sivalingam, Shanmuga Sundar Saravanabhavan, Kavitha Nagarasampatti Palani, and Balasubramanian Natesan
Springer Science and Business Media LLC
J. Narendranath, J. Manokaran, S. Shanmuga Sundar, R. Muruganantham, Ahmed Al Shoaibi, C. Srinivasakannan, and N. Balasubramanian
Informa UK Limited
ABSTRACT In spite of industries making huge investments, for a viable energy saving, still the methods for conservation of energy are lagging and the industries are still in search for alternatives. The major concern that persists with the oil and gas operations is emission of hydrogen sulfide, frequently or inadvertently, during the initial or later stages of the process. Hence, this study focuses on the indirect electrolysis toward the recovery of sulfur and hydrogen from hydrogen sulfide using graphite as both anode and cathode with calomel as reference electrode. The recovered sulfur was analyzed using high-resolution scanning electron microscope was in range of few microns that was in agreement with the X-ray diffraction analysis confirming the crystalline orthorhombic structure of sulfur (JCPDS Card no.89–2600). The cyclic voltammetry experiment was carried out by sweeping the potential between −1.2 and +1.2 V at a scan rate of 50 mVs−1 obtaining a maximum current density of 0.41 A/cm2. Maximum deposition of sulfur observed at pH 13 with a current density of 0.23 A/cm2 and temperature 80°C. The quantity of sulfur recovered by indirect electrolysis was in the range of 2.9 mg with efficiency of 90.44%.Thus an effective method using indirect electrolysis method was successfully used to achieve crystalline sulfur and hydrogen from H2S.
Kamalakannan Vasanthapalaniappan, Kavitha Palani, Shanmuga Sundar Saravanabhavan, Narendranath Jonna, Maharaja Pounsamy, Kannan Natarajan, Yun Suk Huh, and Balasubramanian Natesan
Korean Society of Environmental Engineering
The present study focuses on a novel method to integrate the electro-oxidation process with membrane bioreactor to reduce biofouling and increase the biodegradability index. Here, we used electro-oxidation as pretreatment with membrane bioreactor operating at a current density of 1.5 mA/cm2 with hydraulic retention time at six h. The mixed liquor suspended solids concentration was maintained constant at 3,200 mg/L throughout the experiment for 30 days. The results obtained were promising with the percentage removal of COD, TOC, total nitrogen, and chlorides were in the range of 97%, 90%, 94%, and 15%, respectively, which was comparatively higher than the existing membrane bioreactor. The biodegradable index of treated water was higher, reaching a maximum of 0.6, which is remarkably high compared with 0.3 in a membrane bioreactor. The integrated electro-oxidation process was efficient for the complete removal of pollutants from wastewater, which was confirmed using gas chromatography. In addition, the phytotoxicity test showed a significantly higher quality of treated water compared with that of raw tannery effluent. Hence, our proposed integrated electro-oxidation process can be used to decrease biofouling with increased biodegradability index as a replacement for MBR.
Kannan N, Shanmuga Sundar S, Balaji S, Anil Kumar NV, and Balasubramanian N
Elsevier BV
Anna L. Kaysheva, Tatyana O. Pleshakova, Alexander A. Stepanov, Vadim S. Ziborov, Shanmuga S. Saravanabhavan, Balasubramanian Natesan, Alexander I. Archakov, and Yurii D. Ivanov
Elsevier BV
Shanmuga Sundar Saravanabhavan, Muruganantham Rethinasabapathy, Sarang Zsolt, Aravind Bhat Kalambettu, Sundaravadivel Elumalai, Manokaran Janakiraman, Yun Suk Huh, and Balasubramanian Natesan
Elsevier BV
S. Shanmugasundar, N. Kannan, E. Sundaravadivel, Sarang Zsolt, K. S. Mukunthan, J. Manokaran, J. Narendranath, V. P. Kamalakannan, P. Kavitha, V. Prabhu,et al.
Public Library of Science (PLoS)
Kavitha Nagarasampatti Palani, Darshini Saravanan, Kamalakannan Vasantha Palaniappan, Shanmuga Sundar, and N. Balasubramanian
Springer Science and Business Media LLC
Kannan N, Shanmuga Sundar S, Balaji S, Arul Amuthan, Anil Kumar NV, and Balasubramanian N
Public Library of Science (PLoS)
Background The present study is aimed to evaluate the physiochemical properties and cytotoxicity of mercury-based formulation for the development of anticancer therapeuticals. Methods The elemental and morphological features of the formulation were characterized by FE-SEM, XPS and EDS. The described formulation was evaluated for its cytotoxicity on Hek293 and MCF7 cell lines using MTT assay to study the in vitro effects. The in vivo developmental toxicity was also studied on zebrafish embryos and the lethal concentration (LC50) values were calculated as per the OECD regulations. Results The elemental and morphological characterizations confirmed the presence of mercuric compounds. The particles were spherical and stable with the size ranges between 20 and 80nm. Although the PK formulation contains mercurials it was very effective only to cancerous cells (MCF-7) and it is less toxic to normal cells (HEK 293). The in vivo assessment of developmental toxicity on zebrafish embryo confirmed the safer dosage of 100μg/ml. However, a higher dosage of 1mg/ml led to the malformation of embryos such as pericardial, tail and yolk sac edema. Conclusion The physiochemical characterization of PK formulation confirmed the presence of HgS. The results of both in vitro and in vivo studies showed that the formulation is less toxic. Although the test sample contains mercurials it was very effective against cancerous cells (MCF-7) and it is less toxic to normal cells (HEK 293). Future studies Further studies on effectiveness of the formulation along with inflammatory response in mice models are to be conducted.
Saravanabhavan Sundar, Kalambettu Aravind, Rethinasababathy Muruganantham, and Dharmalingam Sangeetha
Bentham Science Publishers Ltd.
Shanmuga Sundar Saravanabhavan and Sangeetha Dharmalingam
Elsevier BV
Saravanabhavan Shanmuga Sundar and Dharmalingam Sangeetha
Springer Science and Business Media LLC
Saravanabhavan Shanmuga Sundar and Dharmalingam Sangeetha
Springer Science and Business Media LLC