Supratim Mahapatra
@iitbhu.ac.in
Researcher, School of Biochemical Engineering
Indian Institute of Technology (BHU) Varanasi
EDUCATION
CSIR - Indian Institute of Chemical Biology
University of Calcutta
St. Lawrence High School
RESEARCH INTERESTS
Biosensor, Nanotechnology, Cancer Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Supratim Mahapatra, Rohini Kumari, and Pranjal Chandra
Elsevier BV
Supratim Mahapatra and Pranjal Chandra
American Chemical Society (ACS)
Divya, Monalisha Ghosh Dastidar, Supratim Mahapatra, Rohini Kumari, Daphika S. Dkhar, Sharmili Roy, and Pranjal Chandra
Elsevier BV
Supratim Mahapatra and Pranjal Chandra
Elsevier BV
Daphika S Dkhar, Rohini Kumari, Supratim Mahapatra, Divya, and Pranjal Chandra
Wiley
Supratim Mahapatra, Rohini Kumari, and Pranjal Chandra
Elsevier BV
Rahul Kumar, Vinish Ranjan Srivastava, Supratim Mahapatra, Daphika S Dkhar, Rohini Kumari, Kumari Prerna, Vikash Kumar Dubey, and Pranjal Chandra
Ivyspring International Publisher
The focus of this research is to design a bioengineered drug delivery vehicle that is efficient in anti-cancer drug delivery in a controlled manner. The experimental work focuses on constructing a methotrexate-loaded nano lipid polymer system (MTX-NLPHS) that can transport methotrexate (MTX) in MCF-7 cell lines in a controlled manner through endocytosis via phosphatidylcholine. In this experiment, MTX is embedded with polylactic-co-glycolic acid (PLGA) in phosphatidylcholine, which acts as a liposomal framework for regulated drug delivery. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and dynamic light scattering (DLS) were utilized to characterize the developed nanohybrid system. The particle size and encapsulation efficiency of the MTX-NLPHS were found to be 198 ± 8.44 nm and 86.48 ± 0.31 %, respectively, which is suitable for biological applications. The polydispersity index (PDI) and zeta potential of the final system were found to be 0.134 ± 0.048 and -28 ± 3.50 mV, respectively. The lower value of PDI showed the homogenous nature of the particle size, whereas higher negative zeta potential prevented the system from agglomeration. An in vitro release kinetics was conducted to see the release pattern of the system, which took 250 h for 100% drug release This kind of system may carry the drug for a long time in the circulatory system and prevent the drug discharge. Other cell culture assays such as 3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) and reactive oxygen species (ROS) monitoring were used to see the effect of inducers on the cellular system. MTT assay showed cell toxicity of MTX-NLPHS reduced at the lower concentration of the MTX, however, toxicity increased at the higher concentration of the MTX as compared to free MTX. ROS monitoring c revealed more scavenging of ROS using MTX-NLPHS as compared to free MTX. Confocal microscopy suggested the MTX-NLPHS induced more nuclear elongation with cell shrinkage comparatively.
Rohini Kumari, Daphika S. Dkhar, Supratim Mahapatra, Divya, Surinder P. Singh, and Pranjal Chandra
MDPI AG
Metallic dendrites, a class of three-dimensional nanostructured materials, have drawn a lot of interests in the recent years because of their interesting hierarchical structures and distinctive features. They are a hierarchical self-assembled array of primary, secondary, and terminal branches with a plethora of pointed ends, ridges, and edges. These features provide them with larger active surface areas. Due to their enormous active areas, the catalytic activity and conductivity of these nanostructures are higher as compared to other nanomaterials; therefore, they are increasingly used in the fabrication of sensors. This review begins with the properties and various synthetic approaches of nanodendrites. The primary goal of this review is to summarize various nanodendrites-engineered biosensors for monitoring of small molecules, macromolecules, metal ions, and cells in a wide variety of real matrices. Finally, to enlighten future research, the limitations and future potential of these newly discovered materials are discussed.
Divya, Supratim Mahapatra, and Pranjal Chandra
MDPI AG
Creatinine is one of the most common and specific biomarkers for renal diseases, usually found in the serum and urine of humans. Its level is extremely important and critical to know, not only in the case of renal diseases, but also for various other pathological conditions. Hence, detecting creatinine in clinically relevant ranges in a simplistic and personalized manner is interesting and important. In this direction, an optical sensing device has been developed for the simple, point-of-care detection of creatinine. The developed biosensor was able to detect creatinine quantitatively based on optical signals measured through a change in color. The sensor has been integrated with a smartphone to develop a palm-sized device for creatinine analysis in personalized settings. The sensor has been developed following facile chemical modification steps to anchor the creatinine-selective antibody to generate a sensing probe. The fabricated sensor has been thoroughly characterized by FTIR, AFM, and controlled optical analyses. The quantitative analysis is mediated through the reaction between picric acid and creatinine which was detected by the antibody-functionalized sensor probe. The differences in color intensity and creatinine concentrations show an excellent dose-dependent correlation in two different dynamic ranges from 5 to 20 μM and 35 to 400 μM, with a detection limit of 15.37 (±0.79) nM. Several interfering molecules, such as albumin, glucose, ascorbic acid, citric acid, glycine, uric acid, Na+, K+, and Cl−, were tested using the biosensor, in which no cross-reactivity was observed. The utility of the developed system to quantify creatinine in spiked serum samples was validated and the obtained percentage recoveries were found within the range of 89.71–97.30%. The fabricated biosensor was found to be highly reproducible and stable, and it retains its original signal for up to 28 days.
Rahul Kumar, Daphika S. Dkhar, Rohini Kumari, Divya, Supratim Mahapatra, Ananya Srivastava, Vikash Kumar Dubey, and Pranjal Chandra
Wiley
Cancer is one of the major health-related issues affecting the population worldwide and subsequently accounts for the second-largest death. Genetic and epigenetic modifications in oncogenes or tumor suppressor genes affect the regulatory systems that lead to the initiation and progression of cancer. Conventional methods, including chemotherapy/radiotherapy/appropriate combinational therapy and surgery, are being widely used for theranostics of cancer patients. Surgery is useful in treating localized tumors, but it is ineffective in treating metastatic tumors, which spread to other organs and result in a high recurrence rate and death. Also, the therapeutic application of free drugs is related to substantial issues such as poor absorption, solubility, bioavailability, high degradation rate, short shelf-life, and low therapeutic index. Therefore, these issues can be sorted out using nano lipid-based carriers (NLBCs) as promising drug delivery carriers. Still, at most, they fail to achieve site targeted drug delivery and detection. This can be achieved by selecting a specific ligand/antibody for its cognate receptor molecule expressed on the surface of cancer cell. In this review, we have mainly discussed the various types of ligands used to decorate NLBCs. A list of the ligands used to design nanocarriers to target malignant cells has been extensively undertaken. The approved ligand decorated lipid-based nanomedicines with their clinical status has been explained in tabulated form to provide a wider scope to the readers regarding ligand coupled NLBCs. This article is protected by copyright. All rights reserved.
Daphika S. Dkhar, Rohini Kumari, Supratim Mahapatra, Divya, Rahul Kumar, Timir Tripathi, and Pranjal Chandra
Elsevier BV
Rohini Kumari, Daphika S. Dkhar, Supratim Mahapatra, Divya, Rahul Kumar, and Pranjal Chandra
Elsevier BV
Rahul Kumar, Daphika S. Dkhar, Rohini Kumari, Divya, Supratim Mahapatra, Vikash Kumar Dubey, and Pranjal Chandra
Elsevier BV
Namita Bedi, Dhaval K. Srivastava, Arti Srivastava, Supratim Mahapatra, Daphika S. Dkhar, Pranjal Chandra, and Ashutosh Srivastava
Wiley
The Ocean covers two-third of our planet and has great biological heterogeneity. Marine organisms like algae, vertebrates, invertebrates, and microbes are known to provide many natural products with biological activities as well as potent sources of biomaterials for therapeutic, biomedical, biosensors, and climate stabilization. Over the years, the field of biosensors have gained huge attention due to their extraordinary ability to provide early disease diagnosis, rapid detection of various molecules and substances along with long term monitoring. This review aims to focus on the properties and employment of various biomaterials (Carbohydrate polymers, proteins, polyacids etc) of marine origin such as Alginate, Chitin, Chitosan, Fucoidan, Carrageenan, Chondroitin Sulfate (CS), Hyaluronic acid (HA), Collagen, marine pigments, marine nanoparticles, Hydroxyapatite (HAp), Biosilica, lectins, and marine whole cell in the design and development of biosensors. Further, this review also covers the source of such marine biomaterials and their promising evolution in the fabrication of biosensors that are potent to be employed in the biomedical, environmental science and agricultural sciences domains. The use of such fabricated biosensors harness the system with excellent specificity, selectivity, biocompatibility, thermally stable and minimal cost advantages. This article is protected by copyright. All rights reserved.
Rahul Kumar, Neelima Varshney, Supratim Mahapatra, Sanjeev Kumar Mahto, Vikash Kumar Dubey, and Pranjal Chandra
Elsevier BV
Rahul Kumar, Divya, Supratim Mahapatra, Vikash Kumar Dubey, and Pranjal Chandra
Elsevier BV
Aditi Sammi, Divya, Supratim Mahapatra, Rahul Kumar, and Pranjal Chandra
Wiley
Silk is a fibrous protein, has been a part of human lives for centuries and was used as suture and textile material. Silk is mainly produced by members of certain arthropods such as spiders, butterflies, mites, and moths. However, recent technological advances have revolutionized silk as a biomaterial for various applications ranging from heat sensors to robust fibers. The biocompatibility, mechanical resilience, and biodegradability of the material make it a suitable candidate for biomaterials. Silk can also be easily converted into several morphological forms, including fibers, films, sponges, and hydrogels. Provided these abilities, silk have received excellent traction from scientists worldwide for various developments, one of them being its use as a bio-sensor. The diversity of silk materials offers various options, giving scientists the freedom to choose from and personalize them as per their needs. In this review, we foremost look upon the composition, production, properties, and various morphologies of silk. The numerous applications of silk and its derivatives for fabricating biosensors to detect small molecules, macromolecules, and cells have been explored comprehensively. Also, the data from various globally developed sensors using silk have been described into organized tables for each category of molecules, along with their important analytical details. This article is protected by copyright. All rights reserved.
Divya, Daphika S Dkhar, Rohini Kumari, Supratim Mahapatra, Rahul Kumar, and Pranjal Chandra
MDPI AG
Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.
Uday Pratap Azad, Supratim Mahapatra, Divya, Ananya Srivastava, Nagaraj P. Shetti, and Pranjal Chandra
Elsevier
Laxmi S. Killedar, Mahesh M. Shanbhag, Shweta J. Malode, Gangadhar B. Bagihalli, Supratim Mahapatra, Ronald J. Mascarenhas, Nagaraj P. Shetti, and Pranjal Chandra
Elsevier BV
Mahesh M. Shanbhag, Davalasab Ilager, Supratim Mahapatra, Nagaraj P. Shetti, and Pranjal Chandra
Elsevier BV
Divya, Supratim Mahapatra, Vinish Ranjan Srivastava, and Pranjal Chandra
MDPI AG
Recent advancement has been accomplished in the field of biosensors through the modification of cellulose as a nano-engineered matrix material. To date, various techniques have been reported to develop cellulose-based matrices for fabricating different types of biosensors. Trends of involving cellulosic materials in paper-based multiplexing devices and microfluidic analytical technologies have increased because of their disposable, portable, biodegradable properties and cost-effectiveness. Cellulose also has potential in the development of cytosensors because of its various unique properties including biocompatibility. Such cellulose-based sensing devices are also being commercialized for various biomedical diagnostics in recent years and have also been considered as a method of choice in clinical laboratories and personalized diagnosis. In this paper, we have discussed the engineering aspects of cellulose-based sensors that have been reported where such matrices have been used to develop various analytical modules for the detection of small molecules, metal ions, macromolecules, and cells present in a diverse range of samples. Additionally, the developed cellulose-based biosensors and related analytical devices have been comprehensively described in tables with details of the sensing molecule, readout system, sensor configuration, response time, real sample, and their analytical performances.
Suhana Karim, Somali Mukherjee, Supratim Mahapatra, Rumana Parveen, and Debasis Das
Royal Society of Chemistry (RSC)
A facile green hand grinding technique has been adopted towards the design of three nanoscale crystalline materials that serves as an excellent selective bio tracker for intracellular lysosome.
Supratim Mahapatra and Pranjal Chandra
Elsevier BV
The recent outbreak of the coronavirus disease (COVID-19) has left the world clueless. As the WHO declares this new contagion as a pandemic on the 11th of March 2020, the alarming rate of the spawn of the disease in such a short period has disarranged the globe. Standing against this situation researchers are strenuously searching for the key traits responsible for this pandemic. As knowledge regarding the dynamics and host-path interaction of COVID-19 causing Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is currently unknown, the formulation of strategies concerning antiviral treatment, vaccination, and epidemiological control stands crucial. Before designing adequate therapeutic strategies, it is extremely essential to diagnose the disease at the outset as early detection can have a greater impact on building health system capacity. Hence, a comprehensive review of strategies for COVID-19 diagnosis is essential in this existing global situation. In this review, sequentially, we have provided the clinical details along with genetic and proteomic biomarkers related to COVID-19. The article systematically enlightens a clear overview of the clinically adopted techniques for the detection of COVID-19 including oligonucleotide-based molecular detection, Point-of-Care immunodiagnostics, radiographical analysis/sensing system, and newly developed biosensing prototypes having commercial viability. The commercial kits/analytical methods based-sensing strategies have also been tabulated categorically. The critical insights on the developer, commercial brand name, detection methods, technical operational details, detection time, clinical specimen, status, the limit of detection/detection ability have been discussed comprehensively. We believe that this review may provide scientists, clinicians and healthcare manufacturers valuable information regarding the most recent developments/approaches towards COVID-19 diagnosis.
Kuldeep Mahato, Ashutosh Kumar, Buddhadev Purohit, Supratim Mahapatra, Ananya Srivastava, and Pranjal Chandra
Springer Singapore