Sandeep Kumar Swain
@instpath.gov.in
Project Scientist-II
ICMR-National Institute of Child Health and Development Research (Previously as; ICMR-NIP)
Highly capable and motivated biotechnology professional with superior analytical and problem-solving skills with more than 10+ years of research experience. Possesses extensive knowledge in bioassay development, phytochemical analysis, isolation of bioactive molecules and clinical trials in animal models (disease induction/ toxicity study/drug treatment). Expertise in the handling of GC-MS/MS, HPTLC, FTIR, HPLC, TLC analyzer, Lyophilizer, Ultra centrifuges, ELISA/Microplate reader, Gel electrophoresis, WB Analysis study on the protein of interest. Extensive knowledge in diabetes, diabetic complications, various types of leukaemia and their pathophysiology with having 10+ research publications in reputed international peer reviewed scientific journals.
Role:
New product/ingredient Development
Drug application on the animal model
upgradation of assay
Formulation development and improvisation
Bioinformatics application on drug development
EDUCATION
PhD (Biotechnology)
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Plant Science, Cancer Research, Pharmacology (medical)
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Satish Kanhar, Sandeep Kumar Swain, Umesh Chandra Dash, Neelam Meher, and Atish Kumar Sahoo
Elsevier BV
Sandeep Kumar Swain, Bikash Kisan, Neelam Meher, and Atish Kumar Sahoo
Elsevier BV
Satyaprakash Dehury, Priyanka Priyadarsini, Ashirbad Nanda, Debasmita Dubey, Sandeep Kumar Swain, Biswajit Samantaray, Barsha Tripathy, and Satish Kanhar
Horizon E-Publishing Group
Dental caries is the most prevalent oral disease. It is caused by infection of Streptococcus mutans and Candida albicans. It is associated with inflammation of the dental gum. Antimicrobial agents or systemic antibiotics are administered to prevent dental caries. However, the pathogens become drug-resistant to specific antibiotics, so a combinational therapy approach may lead to the management of dental caries. In the current investigation, the peel of Citrus sinensis (L.) Osbeck was evaluated for antimicrobial and anti-inflammatory activities in dental caries. Different fractions of hydroalcohol extract were tested for in vitro antimicrobial activity against S. mutans and C. albicans. Based on the results, methanol fraction was selected for ex-vivo anti-inflammatory activity. The bioactive compounds of the methanol faction were identified by GC-MS. Only selected compounds were subjected to in silico docking analysis towards selective proteins of S. mutans and C. albicans. Amongst all the fractions, the methanol fraction showed significant antimicrobial activity against S. mutans (ZOI, 27 mm; MIC, 0.78 mg/ml; and MBC, 1.56 mg/ml) and C. albicans (ZOI, 29 mm; MIC, 0.39 mg/ml; and MBC, 1.56 mg/ml). Methanol fraction (100 µg/ml) exhibited the highest inhibition of 79.29% than other fractions in the anti-inflammatory study. GC-MS analysis of methanol fraction reported 17 compounds. Out of these, only ten compounds satisfied Lipinski’s rule of five in ADMET analysis and were subjected to in silico docking analysis. The results confirmed that the compounds of methanol fraction have the potential to inhibit the active proteins of dental caries pathogen.
Neelam Meher, Bikash Kisan, Sandeep Kumar Swain, and Atish Kumar Sahoo
Elsevier BV
Sandeep Kumar Swain, Umesh Chandra Dash, Satish Kanhar, and Atish Kumar Sahoo
Elsevier BV
Umesh Chandra Dash, Sandeep Kumar Swain, Atala Bihari Jena, Jagneshwar Dandapat, and Atish Kumar Sahoo
Elsevier BV
Ashirbad Nanda, Rudra Narayan Sahoo, Mahendra Gour, Sandeep Kumar Swain, Debajyoti Das, Amit Kumar Nayak, and Subrata Mallick
Bentham Science Publishers Ltd.
Background: The tear ferning test can be an easy clinical procedure for the evaluation and characterization of the ocular tear film. Objective: The objective of this study was to examine the restoration of tear ferning patterns and reduction of glycosylation peak after amlodipine application in carrageenan-induced conjunctivitis. Methods: At the rabbit’s upper palpebral region, carrageenan was injected for cytokine-mediated conjunctivitis. Ferning pattern and glycosylation of the tear fluid were characterized using various instrumental analyses. The effect of amlodipine was also examined after ocular instillation and flexible docking studies. Results: Optical microscopy showed a disrupted ferning of the tear collected from the inflamed eye. FTIR of the induced tear fluid exhibited peaks within 1000-1200 cm-1, which might be due to the protein glycosylation absent in the normal tear spectrogram. The glycosylation peak reduced significantly in the tear sample collected from the amlodipine-treated group. Corresponding energy dispersive analysis showed the presence of sulphur, indicating protein leakage from the lacrimal gland in the induced group. The disappearance of sulphur from the treated group indicated its remedial effect. The flexible docking studies revealed a stronger binding mode of amlodipine with Interleukin-1β (IL-1β). The reduction in the intensity of the glycosylated peak and the restoration offering are probably due to suppression of IL-1β. Conclusion: This study may be helpful in obtaining primary information for drug discovery to be effective against IL-1β and proving tear fluid as a novel diagnostic biomarker.
Sandeep Kumar Swain, Umesh Chandra Dash, and Atish Kumar Sahoo
Elsevier BV
Deeptimayee Rout, Umesh Chandra Dash, Satish Kanhar, Sandeep Kumar Swain, and Atish Kumar Sahoo
Elsevier BV
Umesh Chandra Dash, Sandeep Kumar Swain, Satish Kanhar, Purusottam Banjare, Partha Pratim Roy, Jagneshwar Dandapat, and Atish Kumar Sahoo
Elsevier BV
Deeptimayee Rout, Umesh Chandra Dash, Satish Kanhar, Sandeep Kumar Swain, and Atish Kumar Sahoo
Elsevier BV
Sandeep Kumar Swain, Umesh Chandra Dash, Satish Kanhar, and Atish Kumar Sahoo
Elsevier BV