Advancing Wildlife Conservation Through Biobanking in South America Carla B. Madelaire, Alexsandra F. Pereira, Adrián J. Sestelo, Aléxia P. Bom-Conselho, Carolina Vaj, et al. Animals, 2025 South America harbors one of the world’s richest biodiversities, yet its wildlife faces escalating threats from climate change and anthropogenic pressures. Biobanking different types of cells and tissues represents an important strategy to preserve genetic diversity and support conservation efforts in the long run. This review highlights the main challenges, opportunities, and future perspectives for biobanking as a conservation tool in South America. Key challenges include technical standardization, funding, and integration with conservation policies. Despite these barriers, recent advances demonstrate the growing importance of biobanking as a complementary tool for safeguarding endangered species and strengthening long-term conservation strategies in the region. The integration of biotechnological approaches into conservation programs positions biobanks as pivotal tools for advancing wildlife management and safeguarding the unique biodiversity of South America.
Evaluation of Echocardiographic, Conventional Electrocardiographic, and Holter Variables in Jaguars (Panthera Onca) Anesthetized with Medetomidine and Ketamine: Implications for Management and Conservation Murillo Daparé Kirnew, Matheus Folgearini Silveira, Roberto Andres Navarrete Ampuero, Ana Paula Rodrigues Simões, Felippe Azzolini, et al. Journal of Zoological and Botanical Gardens, 2025 Jaguars play a crucial role in population control across multiple biomes. They are endangered and protected by in situ and ex situ conservation mechanisms to ensure their conservation. Cardiovascular diseases in wild mammals, including jaguars, often have unclear etiopathogenies, underscoring the need for research into novel hemodynamic parameters. This study evaluates the cardiovascular health of fifteen clinically healthy jaguars using conventional and Holter electrocardiography, non-invasive systemic blood pressure measurement, and echocardiography. Chemical restraint was achieved with medetomidine (0.08–0.1 mg/kg) and ketamine (5 mg/kg), with anesthesia reversed using atipamezole (0.25 mg/kg). The average heart rate was 72 ± 18 bpm, with sinus rhythm in ten animals and sinus arrhythmia in five. Six animals exhibited first and second-degree atrioventricular blocks, one had supraventricular complexes, and another had premature ventricular complexes. Non-invasive systolic blood pressure remained stable at 163 ± 29 mmHg during anesthesia. Echocardiographic examination revealed mitral, tricuspid, pulmonary, and aortic valve insufficiencies via color Doppler. The transmitral flow showed a normal E/A ratio and E` < A`, suggesting a pseudonormal ventricular filling pattern. No significant anesthetic complications were observed, affirming the protocol’s safety. This study provides valuable data, validating the anesthetic protocol and establishing reference cardiovascular values for jaguars, thus paving the way for future research in other veterinary species.
Tongue swallowing in a wild giant anteater (Myrmecophaga tridactyla) Grazielle Soresini, Mario H. Alves, Alessandra Bertassoni, Thyara de Deco-Souza, Diogo H. Freire, et al. Notas Sobre Mamiferos Sudamericanos, 2025 O tamanduá-bandeira Myrmecophaga tridactyla é um mamífero insetívoro Neotropical classifica-do como Vulnerável, cuja especialização alimentar é central para as características biológicas da espécie. Monitoramos um indivíduo de vida livre desde o nascimento até a morte em uma área altamente modificada por atividades humanas, incluindo pastagens e campos agrícolas, onde a fau-na silvestre está exposta a contaminantes provenientes dessas mudanças no uso da terra. Esse tamanduá foi encontrado em uma pastagem apresentando dificuldade respiratória e sem a língua visível na cavidade oral, condição posteriormente confirmada como deglutição da língua por meio de endoscopia. Nenhum sinal de trauma ou doença foi detectado, e as amostras analisadas revela-ram concentrações elevadas de metais traço, sugerindo um problema neurológico possivelmente relacionado à exposição a agroquímicos durante a transição no uso da terra.
Molecular detection of filarioid nematodes (Nematoda: Onchocercidae) in wild mammals from different Brazilian biomes Matheus de Souza Santana, Aline Pedroso Lorenz, Maite Cardoso Coelho da Silva, Gediendson Ribeiro de Araujo, Antonio Carlos Csermak-Junior, et al. Parasitology, 2025 Parasitic nematodes within Onchocercidae are a diverse group transmitted by hematophagous arthropods. This study investigated the molecular occurrence of filarioid nematodes in 93 wild mammals from the Amazon, Cerrado and Pantanal biomes in Brazil, based on the analysis of the mitochondrial genes 12S ribosomal DNA gene (12S rDNA) and cytochrome c oxidase subunit I gene (COI). Conventional polymerase chain reaction (cPCR) targeting the 12S rDNA gene yielded positive results in 14·44% (13/93) of the samples, including 9·86% of jaguars (7/71), 50% of pumas (1/2), 12·5% of giant anteaters (1/8), 50% of ocelots (1/2) and 60% of crab-eating foxes (3/5). Among the 12S-positive samples, 46% (6/13) also tested positive for the COI gene; however, only 1 sequence was suitable for further analysis. Phylogenetic analyses based on 12S gene sequences revealed 4 distinct lineages within the family Onchocercidae. Groups Ia and Ib, composed of Cerrado and Pantanal sequences from jaguars, formed sister clades to Brugia pahangi and Malayfilaria sofiani , respectively. The sequence from the giant anteater (Group Ic) was more divergent, forming a sister clade to species of the genera Malayfilaria, Wuchereria, and Brugia . Group II included sequences closely related to Dirofilaria immitis and D. striata , encompassing samples from crab-eating foxes, ocelots and a puma. These findings suggest that several wild mammal species may serve as reservoirs for previously uncharacterized Onchocercidae nematodes. Our findings expand the existing knowledge on host associations of filarioid nematodes infecting wild mammals from the Pantanal, the Cerrado and the Amazon Rainforest.
Answer to Caravaggi et al. (2021) Gediendson Ribeiro Araujo, Thyara Deco‐Souza, Antônio Carlos Csermak‐Jr, Pedro Nacib Jorge‐Neto, Ronaldo Gonçalves Morato, et al. Methods in Ecology and Evolution, 2021
Use of foot snares to capture large felids Gediendson Ribeiro Araujo, Thyara Deco‐Souza, Ronaldo Gonçalves Morato, Peter G. Crawshaw, Leanes Cruz Silva, et al. Methods in Ecology and Evolution, 2021
Can jaguar (Panthera onca) ovulate without copulation? Pedro Nacib Jorge-Neto, Thiago Cavalheri Luczinski, Gediendson Ribeiro de Araújo, Jorge Aparecido Salomão Júnior, Anneliese de Souza Traldi, et al. Theriogenology, 2020
Advancing Wildlife Conservation Through Biobanking in South America CB Madelaire, AF Pereira, AJ Sestelo, AP Bom-Conselho, C Vaj, ... Animals 15 (22), 3261 , 2025 2025
Molecular detection of filarioid nematodes (Nematoda: Onchocercidae) in wild mammals from different Brazilian biomes M de Souza Santana, AP Lorenz, MCC da Silva, GR de Araujo, ... Parasitology 152 (13), 1387-1397 , 2025 2025
Low human disturbance levels alter movement patterns and induce behavioural changes in an apex predator J Morant, ES González, LR Tambosi, AE de Barros, GR de Araujo, ... Biological Conservation 309, 111278 , 2025 2025 Citations: 6
Tongue swallowing in a wild giant anteater (Myrmecophaga tridactyla) G Soresini, MH Alves, A Bertassoni, T de Deco-Souza, DH Freire, ... Notas sobre Mamíferos Sudamericanos 7 (1) , 2025 2025
Atualidades da reprodução de onças pintadas (Panthera onca) T de Deco-Souza, PN Jorge-Neto, T de Deco Souza, C Schilbach, ... Rev Bras Reprod Anim 49 (1), 202-213 , 2025 2025
Semen collection and cryopreservation from live nurse sharks (Ginglymostoma cirratum): A conservation milestone PN Jorge-Neto, SRB Santos, MDD Carneiro, MF de Goes, RF Valle, ... Animal Reproduction Science 272, 107756 , 2025 2025
Male external genitalia morphology of the solo ex situ pacarana Dynomis branickii (mammalia: Rodentia: Dinomyidae) LS Brandão-Souza, GR de Araujo, T Deco-Souza, DA Rosenfield, ... Zoologischer Anzeiger 310, 17-22 , 2024 2024 Citations: 4
Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm PN Jorge-Neto, F de Moraes Francisco, MDD Carneiro, SRB Santos, ... Theriogenology Wild 4, 100091 , 2024 2024 Citations: 13
Pharmacological induction of parturition in pacarana (Dinomys branickii) LA Requena, GR de Araújo, F Azzolini, MF de Oliveira, T de Deco-Souza, ... Theriogenology Wild 4, 100076 , 2024 2024 Citations: 2
In situ and ex situ jaguar (Panthera onca) reproduction: What do we have so far? T Deco-Souza, GR Araújo, CS Pizzutto, LA Requena, PN Jorge-Neto Theriogenology Wild 4, 100070 , 2024 2024 Citations: 5
A Review on Peanuts Resistance to Aspergillus flavus and Prospects in Susceptibility Genes-Mediated Resistance T Souza Masters Dissertation , 2023 2023 Citations: 1
The Canids T de Deco-Souza, GR de Araujo Assisted Reproduction in Wild Mammals of South America, 51-69 , 2023 2023
The Puma GR de Araujo, T de Deco-Souza Assisted Reproduction in Wild Mammals of South America, 96-108 , 2023 2023
Reproductive evaluation of Luisa, the last jaguar of the Caatinga LA Requena, TC Luczinski, AS Traldi, T Deco-Souza, GR Araújo, ... Animal Reproduction 20 (4), e20230090 , 2023 2023 Citations: 11
Surgical approach in correction of a crack in the gnathotheca of a black swan (Cygnus atratus, Latham, 1790)-First case report L Cazati, MB Ferreira, TBP Faiete, GRD Silva, GS Xavier, TDS Araujo, ... Arquivo Brasileiro de Medicina Veterinária e Zootecnia 75 (4), 709-714 , 2023 2023 Citations: 2
Systemic Histiocytic disorder in a Jaguar (Panthera onca) CC Guizelini, RC Pupin, IR Santos, MB Bandinelli, SP Pavarini, ... Journal of Comparative Pathology 204, 51-54 , 2023 2023 Citations: 3
Cryopreservation of jaguar ( Panthera onca ) sperm cells using different cryoprotectants and different thawing temperatures PN Jorge-Neto, TC Luczinski, GR Araújo, LA Requena, RS Jesus, ... Animal Reproduction 20 (1), e20230009 , 2023 2023 Citations: 15
From livestock production to conservation: innovation for evaluating testicular parenchyma echotexture, and seminal quality in jaguars LA Requena, PN Jorge-Neto, GRD Araújo, L Silveira, ATA Jácomo, ... Rev Bras Reprod Anim [Internet] 47 (2), 438 , 2023 2023 Citations: 2
GPS collars as a tool to uncover environmental crimes in Brazil: The jaguar as a sentinel. AC Csermak, GR De Araújo, CS Pizzutto, T de Deco‐Souza, ... Animal Conservation 26 (2) , 2023 2023 Citations: 12
Xenotransplantation of jaguar (Panthera onca) tissue into NSG mice: an alternative technique for obtaining fibroblasts from road‐killed animals LSB Souza, SR Polizelle, SL Olindo, MCC Silva, GC Vieira, BR Acácio, ... Revista Brasileira de Reprodução Animal 47 (2), 483 , 2023 2023
MOST CITED SCHOLAR PUBLICATIONS
Comparison of semen samples collected from wild and captive jaguars (Panthera onca) by urethral catheterization after pharmacological induction GR de Araujo, TAR de Paula, T de Deco-Souza, RG Morato, LCF Bergo, ... Animal Reproduction Science 195, 1-7 , 2018 2018 Citations: 85
Can jaguar (Panthera onca) ovulate without copulation? PN Jorge-Neto, TC Luczinski, GR de Araújo, JAS Júnior, ... Theriogenology 147, 57-61 , 2020 2020 Citations: 36
Cryptorchidism in free-living jaguar ( Panthera onca ): first case report PN Jorge-Neto, MCC Silva, AC Csermak-Júnior, JA Salmão-Júnior, ... Animal Reproduction 17 (4), e20200555 , 2020 2020 Citations: 26
Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor) T Deco-Souza, TAR de Paula, DS Costa, EP Costa, JBG Barros, ... Pesquisa Veterinária Brasileira 33, 512-516 , 2013 2013 Citations: 26
Use of foot snares to capture large felids GR de Araujo, T de Deco‐Souza, RG Morato, PG Crawshaw Jr, ... Methods in Ecology and Evolution 12 (2), 322-327 , 2021 2021 Citations: 23
Copulatory behavior of the Jaguar Panthera onca (Mammalia: Carnivora: Felidae) PN Jorge-Neto, CS Pizzutto, GR de Araújo, T de Deco-Souza, ... Journal of Threatened Taxa 10 (15), 12933-12939 , 2018 2018 Citations: 22
Uma visão integrada das biotecnologias reprodutivas com o conceito de One Conservation CS Pizzutto, GR de Araújo, AC Csermak-Jr, PN Jorge-Neto, TC Luczinski, ... Rev. Bras. Reprodução Anim 45, 241-245 , 2021 2021 Citations: 21
Can detomidine replace medetomidine for pharmacological semen collection in domestic cats? MCC Silva, KM Ullony, GR Araújo, PN Jorge-Neto, VB Albuquerque, ... Animal Reproduction 18, e20210017 , 2021 2021 Citations: 19
Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes GR de Araujo, TAR de Paula, T de Deco-Souza, R de Morais Garay, ... Animal reproduction science 163, 56-62 , 2015 2015 Citations: 19
Colheita farmacológica de sêmen de onças-pardas (Puma concolor: Mammalia: Carnivora: Felidae) GR Araujo, TAR Paula, T Deco-Souza, RG Morato, LCF Bergo, LC Silva, ... Arquivo Brasileiro de Medicina Veterinária e Zootecnia 72 (02), 437-442 , 2020 2020 Citations: 18
Manejo de população problema através de método contraceptivo cirúrgico em grupos de capivaras (Hydrochoerus hydrochaeris) MV Rodrigues, TAR Paula, VHD Silva, LBC Ferreira, AC Csermak Jr, ... Rev Bras Reprod Anim 41 (4), 710-5 , 2017 2017 Citations: 17
Cryopreservation of jaguar ( Panthera onca ) sperm cells using different cryoprotectants and different thawing temperatures PN Jorge-Neto, TC Luczinski, GR Araújo, LA Requena, RS Jesus, ... Animal Reproduction 20 (1), e20230009 , 2023 2023 Citations: 15
Efficient recovery of in vivo mature and immature oocytes from jaguars (Panthera onca) and pumas (Puma concolor) by Laparoscopic Ovum Pick-Up (LOPU) PN Jorge-Neto, LA Requena, GR de Araújo, A de Souza Traldi, ... Theriogenology Wild 3, 100042 , 2023 2023 Citations: 14
Aspectos do uso territorial por onça parda (Puma concolor), através de monitoramento via satélite, na região do Parque Estadual da Serra do Brigadeiro, MG TAR Paula, GR Araujo, T Deco-Souza, AC Csermak Jr, LCF Bergo, ... Arquivo Brasileiro de Medicina Veterinária e Zootecnia 67, 80-88 , 2015 2015 Citations: 14
Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm PN Jorge-Neto, F de Moraes Francisco, MDD Carneiro, SRB Santos, ... Theriogenology Wild 4, 100091 , 2024 2024 Citations: 13
Reproductive parameters of male crab-eating foxes (Cerdocyon thous) subjected to pharmacological semen collection by urethral catheterization MCC da Silva, PN Jorge-Neto, GM Miranda, AC Csermak-Jr, R Zanella, ... Theriogenology Wild 1, 100004 , 2022 2022 Citations: 13
Fatal infection caused by Cytauxzoon felis in a captive-reared jaguar (Panthera onca) CC Guizelini, CAR Nascimento, JT Echeverria, RL Soares, MM Pimenta, ... International Journal for Parasitology: Parasites and Wildlife 16, 187-190 , 2021 2021 Citations: 13
Field friendly method for wild feline semen cryopreservation GR de Araujo, T de Deco-Souza, LCF Bergo, LC da Silva, RG Morato, ... Journal of Threatened Taxa 12 (5), 15557-15564 , 2020 2020 Citations: 13
GPS collars as a tool to uncover environmental crimes in Brazil: The jaguar as a sentinel. AC Csermak, GR De Araújo, CS Pizzutto, T de Deco‐Souza, ... Animal Conservation 26 (2) , 2023 2023 Citations: 12
Reproductive evaluation of Luisa, the last jaguar of the Caatinga LA Requena, TC Luczinski, AS Traldi, T Deco-Souza, GR Araújo, ... Animal Reproduction 20 (4), e20230090 , 2023 2023 Citations: 11