Tsuyoshi Imasaki

@structure.med.kobe-u.ac.jp

Lectular, Department of Phisiology and Biochemistry
Kobe University Graduate School of Medicine

Tsuyoshi Imasaki


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https://researchid.co/timasaki1

RESEARCH, TEACHING, or OTHER INTERESTS

Structural Biology, Biophysics, Biochemistry, Anatomy
1311

Scholar Citations

17

Scholar h-index

19

Scholar i10-index

RECENT SCHOLAR PUBLICATIONS

  • RAF1 activation drives epithelial cell competition through parallel mitochondrial dysfunction and GAS7-dependent protrusion formation
    L Cheng, H Fujita, T Imasaki, R Nitta, K Aoyama, Y Sakamaki, T Nakata, ...
    2026
  • Structural analysis of a motor with increased mechanical output reveals new transitions in kinesin microtubule motility
    S Shibata, MY Wang, T Imasaki, H Shigematsu, D Ugarte La Torre, Y Wei, ...
    Scientific Reports , 2026
    2026
  • Human spastin functions as an ATPase-independent microtubule nucleator via ring-stack formation
    H Liu, N Matsumoto, T Kaneshiro, T Kato, H Shigematsu, S Kikkawa, ...
    bioRxiv, 2025.11. 28.691112 , 2025
    2025
  • 3169–STRATEGIES TO REGULATE HSC AGING THROUGH THE BONE MARROW MICROENVIRONMENT NICHE BY BRM
    H Suzuki, H Miyachi, H Nishikawa, S Kuno, N Yamada, T Imasaki, ...
    Experimental Hematology 151, 105110 , 2025
    2025
  • Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    H Inaba, T Imasaki, K Aoyama, S Yoshihara, H Takazaki, T Kato, H Goto, ...
    Iscience 28 (6) , 2025
    2025
  • Enhanced axonal transport in large vertebrates: KIF5A adaptations in giraffes and pythons
    T Kambara, L Rao, Y Yamagishi, K Ikeda, D Taniguchi, T Imasaki, ...
    bioRxiv, 2025.05. 04.652084 , 2025
    2025
    Citations: 1
  • Dimerization of GAS2 mediates crosslinking of microtubules and F-actin
    J An, T Imasaki, A Narita, S Niwa, R Sasaki, T Makino, R Nitta, M Kikkawa
    The EMBO Journal 44 (10), 2997 , 2025
    2025
    Citations: 2
  • Dimerization GAS2 mediates microtubule and F-actin crosslinking
    J An, T Imasaki, A Narita, S Niwa, R Sasaki, T Makino, R Nitta, M Kikkawa
    bioRxiv, 2024.08. 19.608523 , 2024
    2024
  • Cryo-electron tomography of the actin cytoskeleton and membrane organization during lamellipodia formation using optogenetics
    H Inaba, T Imasaki, K Aoyama, S Yoshihara, H Takazaki, T Kato, H Goto, ...
    bioRxiv, 2024.08. 13.607852 , 2024
    2024
  • Structural transitions in kinesin minus-end directed microtubule motility
    S Shibata, MY Wang, T Imasaki, H Shigematsu, Y Wei, C Jobichen, ...
    bioRxiv , 2024
    2024
    Citations: 4
  • Structural basis of Irgb6 inactivation by Toxoplasma gondii through the phosphorylation of switch I
    H Okuma, Y Saijo‐Hamano, H Yamada, AA Sherif, E Hashizaki, N Sakai, ...
    Genes to Cells 29 (1), 17-38 , 2024
    2024
  • TEAD1 trapping by the Q353R–Lamin A/C causes dilated cardiomyopathy
    S Yamada, T Ko, M Ito, T Sassa, S Nomura, H Okuma, M Sato, T Imasaki, ...
    Science advances 9 (15), eade7047 , 2023
    2023
    Citations: 26
  • Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12–tubulin complex
    S Taguchi, J Nakano, T Imasaki, T Kita, Y Saijo-Hamano, N Sakai, ...
    Elife 11, e77877 , 2022
    2022
    Citations: 16
  • CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation
    T Imasaki, S Kikkawa, S Niwa, Y Saijo-Hamano, H Shigematsu, ...
    Elife 11, e77365 , 2022
    2022
    Citations: 47
  • Solubility enhancing protein expression systems
    Y TAKAGI, T Imasaki
    US Patent App. 16/641,175 , 2021
    2021
  • CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre
    T Imasaki, S Kikkawa, S Niwa, Y Saijo-Hamano, H Shigematsu, ...
    bioRxiv, 2021.03. 01.433304 , 2021
    2021
    Citations: 2
  • Author Correction: High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity
    W Zhu, A Radadiya, C Bisson, S Wenzel, BE Nordin, F Martínez-Márquez, ...
    Communications biology 2 (1), 438 , 2019
    2019
    Citations: 1
  • High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity
    W Zhu, A Radadiya, C Bisson, S Wenzel, BE Nordin, F Martínez-Márquez, ...
    Communications biology 2 (1), 345 , 2019
    2019
    Citations: 48
  • A practical method for efficient and optimal production of Seleno‐methionine‐labeled recombinant protein complexes in the insect cells
    S Wenzel, T Imasaki, Y Takagi
    Protein Science 28 (4), 808-822 , 2019
    2019
    Citations: 8
  • Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs
    H Shigematsu, T Imasaki, C Doki, T Sumi, M Aoki, T Uchikubo-Kamo, ...
    Journal of Cell Biology 217 (12), 4155-4163 , 2018
    2018
    Citations: 65

MOST CITED SCHOLAR PUBLICATIONS

  • MultiBac: expanding the research toolbox for multiprotein complexes
    C Bieniossek, T Imasaki, Y Takagi, I Berger
    Trends in biochemical sciences 37 (2), 49-57 , 2012
    2012
    Citations: 267
  • Architecture of the Mediator head module
    T Imasaki, G Calero, G Cai, KL Tsai, K Yamada, F Cardelli, ...
    Nature 475 (7355), 240-243 , 2011
    2011
    Citations: 151
  • Mediator structural conservation and implications for the regulation mechanism
    G Cai, T Imasaki, Y Takagi, FJ Asturias
    Structure 17 (4), 559-567 , 2009
    2009
    Citations: 133
  • Mediator head module structure and functional interactions
    G Cai, T Imasaki, K Yamada, F Cardelli, Y Takagi, FJ Asturias
    Nature structural & molecular biology 17 (3), 273-279 , 2010
    2010
    Citations: 128
  • Structural basis for substrate recognition and dissociation by human transportin 1
    T Imasaki, T Shimizu, H Hashimoto, Y Hidaka, S Kose, N Imamoto, ...
    Molecular cell 28 (1), 57-67 , 2007
    2007
    Citations: 119
  • Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs
    H Shigematsu, T Imasaki, C Doki, T Sumi, M Aoki, T Uchikubo-Kamo, ...
    Journal of Cell Biology 217 (12), 4155-4163 , 2018
    2018
    Citations: 65
  • Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
    CM Rogers, JCY Wang, H Noguchi, T Imasaki, Y Takagi, ML Bochman
    Nucleic Acids Research 45 (9), 5217-5230 , 2017
    2017
    Citations: 55
  • High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity
    W Zhu, A Radadiya, C Bisson, S Wenzel, BE Nordin, F Martínez-Márquez, ...
    Communications biology 2 (1), 345 , 2019
    2019
    Citations: 48
  • CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation
    T Imasaki, S Kikkawa, S Niwa, Y Saijo-Hamano, H Shigematsu, ...
    Elife 11, e77365 , 2022
    2022
    Citations: 47
  • Interaction of the mediator head module with RNA polymerase II
    G Cai, YL Chaban, T Imasaki, JA Kovacs, G Calero, PA Penczek, ...
    Structure 20 (5), 899-910 , 2012
    2012
    Citations: 45
  • Substrate specificity of lymphoid-specific tyrosine phosphatase (Lyp) and identification of Src kinase-associated protein of 55 kDa homolog (SKAP-HOM) as a Lyp substrate
    X Yu, M Chen, S Zhang, ZH Yu, JP Sun, L Wang, S Liu, T Imasaki, ...
    Journal of Biological Chemistry 286 (35), 30526-30534 , 2011
    2011
    Citations: 38
  • Crystal structures of type II restriction endonuclease EcoO109I and its complex with cognate DNA
    H Hashimoto, T Shimizu, T Imasaki, M Kato, N Shichijo, K Kita, M Sato
    Journal of Biological Chemistry 280 (7), 5605-5610 , 2005
    2005
    Citations: 37
  • Crystal Structure of the Human Hsmar1 -Derived Transposase Domain in the DNA Repair Enzyme Metnase ,
    KD Goodwin, H He, T Imasaki, SH Lee, MM Georgiadis
    Biochemistry 49 (27), 5705-5713 , 2010
    2010
    Citations: 29
  • Structural insights into the altering function of CRMP2 by phosphorylation
    T Sumi, T Imasaki, M Aoki, N Sakai, E Nitta, M Shirouzu, R Nitta
    Cell structure and function 43 (1), 15-23 , 2018
    2018
    Citations: 28
  • TEAD1 trapping by the Q353R–Lamin A/C causes dilated cardiomyopathy
    S Yamada, T Ko, M Ito, T Sassa, S Nomura, H Okuma, M Sato, T Imasaki, ...
    Science advances 9 (15), eade7047 , 2023
    2023
    Citations: 26
  • Titer estimation for quality control (TEQC) method: A practical approach for optimal production of protein complexes using the baculovirus expression vector system
    T Imasaki, S Wenzel, K Yamada, ML Bryant, Y Takagi
    PLoS One 13 (4), e0195356 , 2018
    2018
    Citations: 21
  • Recent progress in structural biology: lessons from our research history
    R Nitta, T Imasaki, E Nitta
    Microscopy 67 (4), 187-195 , 2018
    2018
    Citations: 17
  • Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells
    M Khanna, T Imasaki, VM Chikwana, S Perez-Miller, GO Hunter, A Mosley, ...
    Protein expression and purification 90 (2), 78-83 , 2013
    2013
    Citations: 17
  • Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12–tubulin complex
    S Taguchi, J Nakano, T Imasaki, T Kita, Y Saijo-Hamano, N Sakai, ...
    Elife 11, e77877 , 2022
    2022
    Citations: 16
  • A practical method for efficient and optimal production of Seleno‐methionine‐labeled recombinant protein complexes in the insect cells
    S Wenzel, T Imasaki, Y Takagi
    Protein Science 28 (4), 808-822 , 2019
    2019
    Citations: 8