Guttikonda Venkateswara Rao

EDUCATION

Bachelor of Dental Surgery-1991
SDM College of Dental Sciences, Karnatak University, Dharwad
Master of Dental Surgery, Oral Pathology & Microbiology- 1995
SDM College of Dental Sciences, Karnatak University, Dharwad

Scopus Publications

A comparative study of salivary flow rate, pH, buffer capacity, total antioxidant capacity, and ferritin levels in children with beta thalassemia major and healthy children
Velagala Daneswari, Guttikonda Venkateshwara Rao, Vem Preethi, Venugopal Reddy, Venumbaka Sivakalyan, and Rajesh Garge
Jaypee Brothers Medical Publishing
Abstract Introduction Thalassemia is a genetically diverse group of disease which occurs due to an imbalance in the production of the alpha and beta chains with ensuring chronic hemolysis, ineffective erythropoiesis, and iron overload. Aim and objective The main aim of the study is to compare the salivary flow rate, pH, buffer capacity, total antioxidant capacity, and ferritin levels in children with beta thalassemia major and healthy children. Materials and methods The present study includes 40 children consisting of 20 beta thalassemia major and 20 healthy children. Unstimulated saliva was collected by the spitting method. The salivary flow rate, pH, buffer capacity, total antioxidant capacity, and ferritin levels were evaluated. Results Salivary ferritin levels were found to be higher in children with beta thalassemia major. Conclusion Iron overload is also known as hemochromatosis which is one of the complications of thalassemia. How to cite this article Rao GV, Preethi V, Daneswari V, et al. A Comparative Study of Salivary Flow Rate, pH, Buffer Capacity, Total Antioxidant Capacity, and Ferritin Levels in Children with Beta Thalassemia Major and Healthy Children. Int J Clin Pediatr Dent 2021;14(3):342-348.


Salivary malondialdehyde in oral submucous fibrosis - A marker for oxidative damage
ShyamRaj Ganta, Samatha Chittemsetti, Taneeru Sravya, and VenkateswaraRao Guttikonda
Medknow
Background: Oral submucous fibrosis (OSMF) is a potentially malignant disorder of oral mucosa and its malignant transformation rate accounts to about 7%–13%. Oxidative damage and lipid peroxidation plays an important role in OSMF. Lipid peroxidation has not been widely investigated in OSMF patients with respect to clinical staging and histopathological grading. As human saliva is a diagnostic fluid which can be obtained in a noninvasive procedure as compared to the blood for serum analysis, the present study was aimed at evaluating the salivary malondialdehyde (MDA) levels in OSMF and comparison with respect to clinical staging and histopathological grading. Aim: This study aims to evaluate salivary MDA levels in OSMF and compare the levels with respect to clinical and histopathological grading systems. Materials and Methods: Forty cases of clinically diagnosed and histopathologically proven cases of OSMF were included for the purpose of this study. As controls 40 age-matched individuals without any systemic disease were selected. Unstimulated whole saliva was collected from each individual, centrifuged and frozen at − 20°C until analysis. Lipid peroxidation products MDA were analyzed by thiobarbituric acid reaction. Results: Salivary MDA levels were significantly increased in OSMF patients compared to controls. The progressively increased salivary MDA levels showed a positive correlation with the clinical stages and histopathological grades of OSMF and the results were statistically significant. Conclusion: The increased salivary MDA levels in OSMF patients compared to the control group suggests an increased oxidative stress levels in the potentially malignant disorders such as OSMF. The mean salivary MDA levels were increased significantly as the clinical stage and histopathological grade of OSMF advances, suggesting MDA to be used as a reliable biochemical marker and also a prognostic marker to assess the extent of oxidative damage in OSMF.


Assessing the accuracy of Cameriere’s Indian-specific formula for age estimation on right and left sides of orthopantomogram
Purnima Vadla, R. Surekha, Guttikonda Venkateswara Rao, G. Deepthi, Sayani Naveen, and Chippalapally Arun Kumar
Springer Science and Business Media LLC
Abstract Background Age estimation is of prime importance in forensic science and clinical dentistry. In children, age estimation can be done by skeletal maturity indicators like hand-wrist radiographs and dental age estimation. Skeletal maturity indicators compared with dental age estimation is limited as they are influenced by various environmental parameters, whereas calcification of teeth depends on genes rather than environmental factors. Many of the dental age estimation methods use extracted teeth, which are quite invasive, whereas Cameriere’s method is a recently introduced radiographic method first in European population; where in age estimation is done using open apices of teeth by orthopantomogram (OPG). Indian-specific formula was introduced later using permanent mandibular teeth on left side of jaw. The present study aimed to estimate the age and also to determine the accuracy of Cameriere’s method using Indian-specific formula on both right and left sides of mandible in Khammam population of South India and also to determine the side which can be efficient in determining age. Methodology The present study comprised radiographs of 50 subjects (25 boys and 25 girls) ranging from 5 to 15 years. The soft copies of the radiographs of selected subjects were retrieved from the computer attached to the digital orthopantomogram machine (Orthophos XG5; Sirona Dental Systems). The 7 left and right permanent mandibular teeth were assessed in OPGs. The number of teeth with closed apical and with open apical ends of roots was examined and measured. The values were tabulated based on the Cameriere method of age estimation using Indian-specific formula. Statistical analysis was done using paired t test and Karl Pearson’s correlation coefficient test. Results Comparison of dental age with chronological age in males showed non-significant results on both left and right sides of the OPG with a p value of 0.3765 and 0.3045, respectively. Likewise in females, p values of 0.2167 and 0.8089 were noted. When males and females were compared, non-significant results were obtained with a p value of 0.1613 in the age estimated on the left side of the OPG and a p value of 0.4322 on the right side of the OPG. Correlation test showed that left side of the OPG showed better results in determining age than the right side of the OPG with an r value of 0.9982 and 0.9485 in males and females, respectively. Conclusion Cameriere’s method of age estimation using Indian-specific formula proves to be an accurate and a reliable method which can be used to assess the chronological age of individuals. There is also a good correlation found between the chronological age and dental age of younger age group individuals.


Immunohistochemical expression of kallikrein 7 in oral squamous cell carcinoma
Dodda Kumar, Y. Sivaranjani and G. Rao

Background and Objectives: The kallikrein (KLK) family of genes consists of 15 members, many of which are highly expressed in number of cancers compared to their normal parent tissues. KLK7 was initially characterized as an enzyme implicated in the degradation of intercellular cohesive structures in the stratum corneum of stratified squamous epithelia, preceding desquamation in the skin. It catalyzes the degradation of desmosomes in the outermost layer of skin and permits cell shedding to take place at the skin surface. Overexpression of KLK7 in tumor cells has been reported to significantly enhance the invasive potential in intracranial malignancies and ovarian cancer cells. Thus, KLK7 could contribute to the degradation of extracellular matrices in oral squamous cell carcinoma (OSCC) tissues, promoting invasion of neoplastic cells locally and facilitating metastasis to regional lymph nodes. The objectives of the present study were to compare the expression of KLK 7 in normal subjects and patients with OSCC, to correlate the expression of KLK 7 with respect to the clinical staging of OSCC and to evaluate the expression of KLK 7with respect to different histopathological grades of OSCC. Materials and Methods: Thirty cases of OSCC were staged clinically and graded histopathologically. The immunohistochemical method was used to detect the expression of KLK 7 in OSCC. The scores obtained were documented and compared statistically. Results: KLK 7 immunoreactivity was noticed in all cases of OSCC. A statistically significant difference was observed in immunoreactivity of KLK 7 between the normal and OSCC (P = 0.0001*) and in different histopathological grades (P = 0.0001*) and in different clinical stages (P = 0.0127*) of OSCC using Kruskal–Wallis analysis of variance test. Conclusion: The KLK 7 immunoexpression histopathologically increased from low grade to high grade and clinically from Stage 1 to Stage 4 in OSCC. Hence, increased expression of KLK 7 may be related to poor prognosis in patients with OSCC.


Estimation of serum pyruvic acid levels in oral squamous cell carcinoma
Priyanka Guduguntla and V. Guttikonda

Introduction: Cancer cells generally exhibit increased glycolysis for adenosine triphosphate generation (the Warburg effect) due to mitochondrial respiration injury and hypoxia, which are frequently associated with resistance to therapeutic agents. Pyruvic acid is produced as an end product of glycolysis along with lactic acid. At room temperature, lactic acid converts into pyruvic acid as it is chemically unstable. Aim: To evaluate the serum pyruvic acid levels in patients with oral squamous cell carcinoma (OSCC). Materials and Methods: Thirty clinically and histopathologically confirmed cases of OSCC were included in the study. The cases were staged clinically (tumor–node–metastasis staging) and graded histopathologically (modified Broder's classification). A control group of 30 age-matched individuals with no systemic diseases were selected and the serum levels of pyruvic acid were measured and the absorbance was read using a spectrophotometer at a wavelength of 540 nm. The results were evaluated statistically and discussed. Results: Pair-wise comparison of clinical stages and histological grades of OSCC was done using Tukey's multiple post hoc procedure. The increase in mean serum pyruvic acid levels between any two groups and grades was found to be statistically significant respectively (P<0.05). Conclusion: Serum pyruvic acid levels gradually increased from individuals without OSCC to individuals with OSCC. Higher levels of serum pyruvic acid were seen with increasing clinical stage and the mean serum pyruvic acid levels were also found to be significantly increasing with advancing histopathological grades of OSCC.


Recurrent adenoid cystic carcinoma of paranasal sinuses: A rare case report
SarahAbdul Wahed, Katakam Pradeepthi, MadalaJaya Kiran, and GuttikondaVenkateswara Rao
Medknow
Adenoid cystic carcinoma (ACC) is a distinct salivary gland neoplasm that predominantly occurs in the minor salivary gland. ACC is an uncommon tumor that progress slowly affecting 5% of paranasal sinuses with high distant metastasis rate in advance stages. This salivary gland tumor is difficult to diagnose both clinically and histopathologically due to its indolent presentation and diverse histopathological patterns. Hence, immunohistochemistry plays a decisive role in diagnosing this tumor. A recurrent case of ACC of paranasal sinuses in the maxillary sinus of a 41-year-old female patient without any extraoral abnormality is reported.


Stathmin! An Immunohistochemical Analysis of the Novel Marker in Oral Squamous Cell Carcinoma and Oral Leukoplakia
Purnima Vadla, Sivaranjani Yeluri, G Deepthi, Venkateswara Rao Guttikonda, Sravya Taneeru, and Srikanth Naramala
EpiSmart Science Vector Ltd
Background: Stathmin is an intracellular phosphoprotein that controls the microtubule dynamics by further regulating proper attachment and alignment of chromosomes in a dividing cell. Thus, any mutation or aberrantly expressed protein that reduces the fidelity of spindle assembly will enhance chromosomal instability contributing to aneuploidy. Oral Squamous Cell Carcinoma is an extensively studied malignancy that occurs due to accumulated genetic changes due to carcinogens. The current study is done to evaluate the stathmin role and its expression in OSCC and Oral epithelial dysplasia (OED). Objective: The aim of the present study is to evaluate the role of stathmin in OSCC and Oral dysplasia and also to correlate the expression of Stathmin with respect to the different histopathological grades of OED and OSCC. Materials and Methods: 30 neutral buffered formalin fixed, paraffin embedded (FFPE) tissues of Oral Leukoplakia/OED and 30 FFPE tissues of OSCC were subjected to immunohistochemistry with stathmin antibody. Five fields of each case with 300 cells were examined and a mean percentage of positive–stained slides were determined. The percentages were recorded accordingly with their respective histological grades. The results were analysed statistically. Results: The results of the present study demonstrated higher mean values of stathmin in tissues with OSCC (2.50) compared to leukoplakia (2.11) and normal tissues (0.00) with a high level of statistical significance (0.0001). There is also an increase in the percentage levels of stathmin with increase in the histological grade of differentiation in OSCC as well as leukoplakia. Conclusion: The present study found a statistical correlation between increased grades of the disease with expression levels of stathmin. This confirms that stathmin expression can contribute to disease progression and that stathmin might have a potential role as an early diagnostic biomarker and can be a therapeutic target for OSCC.


Sclerosing polycystic adenosis of hard palate: A rare entity in salivary glands
Priyanka Guduguntla, Rajani Korlepara, and VenkateswaraRao Guttikonda
Medknow
Sclerosing polycystic adenosis (SPA) is a rare neoplasm of salivary glands, with a striking resemblance to the benign fibrocystic disease of the breast. The most common site of occurrence is the parotid gland. However, submandibular gland, minor salivary glands, and buccal mucosa may also be affected. It shows a slight female predilection and occurs over a wide age range. The exact nature of the disease is unknown but is postulated to be a neoplasm recently. The treatment is effective by localized surgical excision, and recurrence is rare. To the best of our knowledge, there is only one case report of SPA involving palate. Here, we describe an additional case report of SPA occurring in the palatal region of a 49-year-old male patient.


Rhinocerebral mucormycosis associated with actinomycosis in a diabetic patient: A rare presentation
SandaUsha Rani, Yeluri Sivaranjani, ManchikatlaPraveen Kumar, and GuttikondaVenkateswara Rao
Medknow
Mucormycosis is an opportunistic fulminant fungal infection which mainly affects the immunocompromised individuals. It begins in the nose and paranasal sinuses due to the inhalation of fungal spores. The common predisposing factors include diabetes mellitus and immunosuppression. Actinomycosis is a bacterial infection caused by nonspore-forming, anaerobic or microaerophilic bacterial species of the genus Actinomyces. It is a suppurative and chronic granulomatous disease characterized by abscess formation, tissue fibrosis and draining sinuses rarely diagnosed in humans. A case of rhinocerebral mucormycosis associated with actinomycosis of the maxilla involving the palate in an uncontrolled diabetic patient is reported.


Solitary plasmacytoma of mandible: A rare entity
Samatha Chittemsetti, V. Guttikonda, Taneeru Sravya and P. K. Manchikatla

Plasmacytoma is a discrete, unifocal, monoclonal neoplastic proliferation of the plasma cells. It may present as one of the three distinct clinical entities: multiple myeloma (MM), solitary plasmacytoma of bone and extramedullary plasmacytoma. Solitary bone plasmacytoma accounts for 3% of all plasma cell neoplasms with approximately 50% of the cases transforming into MM. It is frequently seen in vertebrae and secondarily in long bones. Its presence in jaws is extremely rare and when it is seen, retromolar area, angle and ramus of the mandible are most common sites of occurrence. A comprehensive clinical, radiological, histological and immunohistochemical features of solitary plasmacytoma of the mandible in a 46-year-old female patient is reported.


Estimation of serum sialic acid in oral submucous fibrosis and oral squamous cell carcinoma
Samatha Chittemsetti, P. K. Manchikatla and V. Guttikonda

Background and Objectives: Sialic acid (SA) N-acetyl neuraminic acid is a negatively charged 9-carbon monosaccharide, commonly attached to the nonreducing residues of carbohydrate chains of glycoconjugates by glycosidic linkage. SA is widely distributed in glycoproteins (GPs) of cell membrane. The alterations in GPs start at an early stage of tumorigenesis. Hence, the aim of the present study is to evaluate the levels of serum SA in normal individuals, in patients with oral submucous fibrosis (OSMF), oral squamous cell carcinoma (OSCC), and compare the levels with respect to the clinical staging and histological grading. Materials and Methods: A total of 90 individuals were selected for the purpose of the study. Thirty cases of clinically diagnosed and histopathologically confirmed cases of OSMF and OSCC each were included. A control group of 30 age and gender-matched individuals with no systemic diseases were selected. Serum levels of SA were measured based on the reaction between SA and ninhydrin. The absorbance was read using a spectrophotometer. Results: Serum SA levels were significantly increased in OSMF, OSCC patients as compared with controls. When multiple comparison was done using post hoc Tukey test, there is a statistically significant difference between clinical staging and histopathological grading of OSMF and OSCC (P < 0.05). Conclusion: The serum SA levels in OSMF and OSCC patients were increased as compared with controls suggesting that, it can be used as a reliable biomarker for prognostic evaluation, and also give a clue about the amount of tumor burden in the individual.


Demystifying the nature of hard tissues in odontogenic tumors using Modified Gallego's stain: A preliminary study




Basaloid squamous cell carcinoma: Report of two rare cases and review of literature
Kalavani Peddapelli, GuttikondaVenkateswara Rao, Taneeru Sravya, and Sravya Ravipati
Medknow
Basaloid squamous cell carcinoma (BSCC) is a histologically distinct variant of SCC in the head-and-neck region it occurs most commonly in older men with a history of heavy smoking and alcohol abuse and usually presents as a high stage disease with widespread metastases and hence poor prognosis. BSCC is believed to arise from a totipotential primitive cell in the basal layer of the surface epithelium or from the salivary duct lining epithelium. BSCC is an uncommon tumor with a predilection for the upper aerodigestive tract, is a distinct variant of squamous carcinoma, due to its unique histological features and ominous clinical behavior. In the oral cavity, BSCC has a predilection for the tongue, followed by the floor of the mouth, palate, buccal mucosa, retromolar trigone and gingiva. In the oral cavity, retromolar trigone/gingiva is very rare sites and only a few cases have been reported in the literature. This paper reports an additional two cases of BSCC, one reported in the retromolar region and other reported on the gingiva.


Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study
Taneeru Sravya, G. Rao, Masabattula Geetha Kumari, Yerraguntla Vidya Sagar, Y. Sivaranjani and K. Sudheerkanth

Background: Xylene is synthetic hydrocarbon produced from coal tar known for its wide usage as universal solvent which has many hazardous effects. The aim of this study is to compare the efficacy of xylene-free hematoxylin and eosin (H and E) sections with conventional H and E sections. Materials and Methods: The study included ninety paraffin-embedded tissue blocks. Of these, sixty blocks were processed with sesame oil (xylene alternative) and thirty blocks with xylene. The study sample was divided into three groups. Sixty sections which are taken from sesame oil-processed blocks were stained with xylene-free H and E staining method. In xylene-free staining method, 95% diluted lemon water (Group A) and 1.7% dish washing solution (DWS, Group B) were used as deparaffinizing agents whereas the remaining 30 sections were processed with xylene and stained with conventional H and E staining method (Group C). Slides were scored for the following parameters: (i) nuclear staining (adequate = score 1, inadequate = score 0), (ii) cytoplasmic staining (adequate = score 1, inadequate = score 0), (iii) uniformity (present = score 1, absent = score 0), (iv) clarity (present = score 1, absent = score 0) and (v) intensity (present = score 1, absent = score 0). Score ≤2 was considered inadequate for diagnosis while scores 3–5 were considered adequate for diagnosis. Results: Adequate nuclear staining was noted in 90% of sections of Group A and 100% each in Group B and Group C (P < 0.05); adequate cytoplasmic staining in 96.7% in Group A and 100% each in Group B and Group C (P > 0.05); adequate uniformity of staining in 53.3% of sections of Group A, 70% in Group B and 83.3% in Group C (P < 0.05); adequate clarity of staining in 73.3% sections of Group A, 80% in Group B and 83.3% in Group C (P > 0.05) and adequate intensity of staining in 76.7% sections of Group A, 93.3% in Group B and 100% in Group C (P < 0.05). Group C sections stained adequate for diagnosis (93.3%) followed by Group B (88.7%) and Group A (78%; P< 0.05). Conclusion: Tissues processed with sesame oil and stained using 1.7% DWS were found to be effective alternative to xylene.


Natural substitutes for formalin: A boon to histopathology!!
Samatha Chittemsetti, S. Nallamala, Taneeru Sravya, V. Guttikonda, P. K. Manchikatla and S. Kondamari

Introduction: In routine tissue processing, formalin has been proved as efficient as fixative since inception and hazards associated with it are of major safety and health concern. Aim: The aim of this study is to compare the efficacy of natural fixatives such as jaggery and Khandsari over formalin. Materials and Methods: Ninety normal tissue specimen collected during minor oral surgical procedures were included in this study. Thirty specimen each were fixed in 30% jaggery (Group-A), 30% Khandsari (Group-B) and 10% formalin (Group-C). The slides obtained after tissue processing were analyzed for the quality of fixation. The tissue sections were assessed for cellular outline, cytoplasmic details, nuclear details, staining quality and overall morphology. Each criterion was rated on a scale of 1–4. (one for poor and four for excellent). The study was double-blinded and subjected to the Kruskal–Wallis ANOVA and Chi-square test. Results: The cellular outline is excellent in 90% (Group-C) followed by 36.67% (Group-B) of specimens. With respect to cytoplasmic staining 83.33% (Group-C) of tissues showed excellent results followed by 60% (Group-B), 33.33% (Group-A). Nuclear details are excellent in 86.67% (Group-C) followed by 83.33% (Group-B), 36.67% (Group-A) of specimens. With respect to staining quality 93.33% (Group-C) followed by 50% (Group-B), 26.67% (Group-A). Overall morphology is excellent in 90% (Group-C) followed by 46.67% (Group-B). Conclusion: In the present study, Khandsari was on par with formalin and our effort of using Khandsari and jaggery for tissue fixation in human oral tissues yielded good results.


Polymorphous adenocarcinoma of the oral cavity: A skeptical case mimicking lobular carcinoma of breast and gastric carcinoma
Purnima Vadla, Sankeerthana Pathipaka, Jayakiran Madala, and VenkateswaraRao Guttikonda
Medknow
Polymorphous adenocarcinoma (PAC) is a distinctive salivary gland neoplasm that predominantly occurs in the minor salivary glands. The tumor is characterized by cytological uniformity, morphological diversity, an infiltrative growth pattern and low metastatic potential. It presents as an asymptomatic, slow- growing mass within the oral cavity. This salivary gland tumor is difficult to diagnose both clinically and histopathologically due to its indolent presentation and diverse architectural pattern which includes various microscopic patterns namely- solid, ductal-tubular, cribriform, trabecular and single-file growth. Hence, Immunohistochemistry plays a very important role in diagnosing this tumor. We hereby report a rare case of PAC occurring in a 50yr old female patient presenting with a lesion in right upper posterior part of alveolus extending to the hard palate.


Ameloblastoma arising in the wall of dentigerous cyst: Report of a rare entity
SudheerKanth Kondamari, Sravya Taneeru, VenkateswaraRao Guttikonda, and GeethaKumari Masabattula
Medknow
Dentigerous cyst (DC) is a developmental odontogenic cyst that encloses the crown of an unerupted tooth by expansion of its follicle with accumulation of fluid between the reduced enamel epithelium and the tooth crown and is attached to the neck of the tooth. The lining of DCs shows a potential for neoplastic transformation to ameloblastoma, squamous cell carcinoma, and mucoepidermoid carcinoma. Here, we report a rare case of an ameloblastoma arising in the wall of a DC.


Mucormycosis in a diabetic patient: A case report with an insight into its pathophysiology
SyedaNeelam Afroze, Rajani Korlepara, GuttikondaVenkateswara Rao, and Jayakiran Madala
Medknow
Mucormycosis is one of the most rapidly progressing and fulminant forms of fungal infection which usually begins in the nose and paranasal sinuses following inhalation of fungal spores. It is caused by organisms of the subphylum Mucormycotina, including genera as Absidia, Mucor, Rhizomucor, and Rhizopus. The incidence of mucormycosis is approximately 1.7 cases per 1,000,000 inhabitants per year. Mucormycosis affecting the maxilla is rare because of rich blood vessel supply of maxillofacial areas although more virulent fungi such as Mucor can overcome this difficulty. The common form of this infection is seen in the rhinomaxillary region and in patients with immunocompromised state such as diabetes. Hence, early diagnosis of this potentially life-threatening disease and prompt treatment is of prime importance in reducing the mortality rate.


Early diagnosis of an isolated primary peripheral T-cell lymphoma masquerading as massive gingival enlargement in a pediatric patient
Sravani Ghattamaneni, VenkateswaraRao Guttikonda, Sivaranjani Yeluri, and Rajani Kolipara
Medknow
Lymphomas are malignant neoplasm of the lymphocyte cell lines, classified as either Hodgkin's or non-Hodgkin's lymphoma (NHL). NHL comprises a heterogeneous group of lymphoid neoplasm arising from B-cell, T-cell or natural killer cell with a spectrum of behavior ranging from relatively indolent to highly aggressive and potentially fatal. Peripheral T-cell lymphoma, a variant of NHL, is a disease characterized by the presence of diffuse lymphadenopathy, extranodal involvement, classical B symptoms such as fever (>100.4°F) for 3 consecutive days, weight loss exceeding 10% of body weight in 6 months and drenching night sweats with a tendency for recurrence. Among NHLs, extranodal presentations are relatively common. Extranodal presentation particularly in the oral cavity is very rare and may misinterpret the diagnosis. Lesions of this type should be cautiously dealt especially in pediatric patients and young adolescents. The present case report is about an atypical presentation of disease process in a 15-year-old male patient, which was diagnosed early with the help of a combination of histopathology and immunohistochemistry techniques.


Modified ultrafast Papanicolaou staining technique: A comparative study
Moni Thakur and VenkateswaraRao Guttikonda
Medknow
Introduction: Ultrafast Papanicolaou stain (UFP) was introduced as a hybrid of Romanowsky and Papanicolaou (PAP) stain. It enhances the quality and reduces the time. In the present study, a modified staining technique was adapted where Gill's Hematoxylin was replaced by Harris Hematoxylin. Aims: The aim of the study was to assess the use of the modified ultrafast Papanicolaou (MUFP) stain for fine needle aspiration cytology (FNAC) of head and neck swellings in comparison with the routine PAP stain, hematoxylin and eosin (H and E), and Giemsa. Materials and Methods: Forty FNACs of head and neck swellings were collected. FNAC procedure was performed by standard method; two smears were fixed in 95% propanol and stained with PAP and H and E. Two smears were air dried, 1 was stained with Giemsa, and 1 was rehydrated with normal saline, fixed in alcoholic formalin, and stained with MUFP. Four parameters were considered and scored background, cell morphology, nuclear staining, and overall staining pattern). Results: The quality of MUFP smears were better when compared to routine PAP, H and E, and Giemsa, and was statistically significant by Wilcoxon matched pair test. Conclusions: MUFP stain in comparison to routine PAP, H and E, and Giemsa provides an excellent and suitable alterative in cytological staining for the study of various organs.


Non Calcifying Type of Calcifying Epithelial Odontogenic Tumor: An Unusual Case Report with Special Emphasis on Histogenesis of Calcifications
Sravya Taneeru, Venkateswara Rao Guttikonda, Rajani Korlepara, Rajasekhar Gaddipati, and Vinay Kumar Kundoor
Springer Science and Business Media LLC



Unicystic ameloblastoma in conjunction with peripheral ameloblastoma: A unique case report presenting with diverse histological patterns
Sravani Ghattamaneni, Shilpa Nallamala, and VenkateswaraRao Guttikonda
Medknow
Ameloblastomas are benign epithelial odontogenic neoplasms which are locally aggressive with an insidious growth pattern. Based on the clinical, radiographic, histopathology and behavioral and prognostic aspects, four variants of ameloblastoma are distinguished. They include solid/multicystic ameloblastoma, unicystic ameloblastoma (UA), peripheral ameloblastoma (PA) and desmoplastic ameloblastoma. UA and PA are two variants that have no clinical or radiological resemblance to each other. A case of simultaneous occurrence of these two lesions displaying an array of different histopathological entities together, with a note on pathological insight, has been reported in a 59-year-old adult male patient.


Estimation of hemoglobin, serum iron, total iron-binding capacity and serum ferritin levels in oral submucous fibrosis: A clinicopathological study
Moni Thakur and VenkateswaraRao Guttikonda
Medknow
Introduction: Oral submucous fibrosis (OSMF) is also considered as an Asian version of sideropenic dysphagia, wherein chronic iron deficiency leads to mucosal susceptibility to irritants, such as chillies and areca nut products. Hemoglobin (Hb) levels, in particular, serum iron levels, are considered as biochemical indicators for nutritional assessment. Serum iron content can thus be a predictor for the progression of the condition, and there appears to be an association between serum iron content and oral carcinogenesis. Therefore, biochemical assessment of oral precancerous conditions may help in early diagnosis and prognosis. Hence, the present study was aimed to evaluate Hb, serum iron, total iron-binding capacity (TIBC) and serum ferritin in OSMF patients. Materials and Methods: Forty cases of OSMF were staged clinically and graded histopathologically to assess the levels of Hb, serum iron, TIBC and serum ferritin and compared with normal subjects. The results were compared using t-test, ANOVA, and Tukeys multiple post hoc procedures. Results: It was observed that the levels of Hb, serum iron and serum ferritin levels gradually reduced in OSMF patients compared with controls and as the clinical stage and histological grade of OSMF progressed with a statistically significant P < 0.05. It was observed that the levels of TIBC gradually increased in OSMF patients compared with controls and as the clinical stage and histological grade of OSMF progressed with a statistically significant P < 0.05. Conclusion: The Hb, serum iron and serum ferritin levels in OSMF patients were reduced and TIBC increased as compared with controls and as the clinical stage and histological grade of OSMF advances indicating their role as a reliable biochemical indicator.


Inflammatory myofibroblastic tumor of mandible: A rare case report and review of literature
Rajani Korlepara, VenkateswaraRao Guttikonda, Jayakiran Madala, and Sravya Taneeru
Medknow
Inflammatory pseudotumor is a term given to different neoplastic and nonneoplastic entities that have a common histological appearance, which comprises spindle cell proliferation with a prominent chronic inflammatory cell infiltrate. Inflammatory myofibroblastic tumor (IMT) is an uncommon lesion with distinctive clinical, pathological and molecular features and is considered to be pseudotumor for the past two decades due to its appearance. IMT is an intermediate soft tissue tumor which was first observed in lungs. It was named as IMT because it mimics a malignant neoplasm clinically, radiologically and histopathologically. The most common sites are lungs, liver and gastrointestinal tract. IMT in head and neck region is exceptionally rare and the sites reported include gingiva, tongue, hard palate, mandible, buccal mucosa and submandibular salivary gland. Till now, 8 cases of intramandibular IMT were reported. Here, we report an additional case of intramandibular IMT in a 20-year-old male patient.


Oral adenosquamous carcinoma: Report of a rare entity with a special insight on its histochemistry
Taneeru Sravya, GuttikondaVenkateswara Rao, ManchikatlaPraveen Kumar, and K Sudheerkanth
Medknow
Adenosquamous carcinoma (ASC) of the head and neck (H and N) is an aggressive variant of squamous cell carcinoma (SCC). They are described as SCC subtype with high infiltrative capacity and also presents with dual histomorphology, having both squamous and glandular cell components. ASC of the H and N region is considered as a controversial tumor, as it is similar to salivary gland mucoepidermoid carcinoma. It has been described in a variety of body sites, including uterine cervix, lung and pancreas. ASC rarely develops in the upper aerodigestive tract, particularly in the oral cavity. The affected sites in oral cavity include palate, tonsillar pillar areas and floor of the mouth. To the best of our knowledge in the literature, only 17 cases of ASC in the floor of the mouth have been reported. Hereby, we report an additional case of ASC occurring in the floor of the mouth in a 70-year-old male patient.

RECENT SCHOLAR PUBLICATIONS

Evaluation of biosafe alternative to eosin in hematoxylin and eosin staining procedure: A comparative study
K Pradeepthi, K Rajani, GV Rao, T Sravya, SA Wahed, J Sailaja
Journal of Oral and Maxillofacial Pathology 27 (2), 424-425 2023



Digitilized Radiographic Analysis of Coronal Pulp for Age Estimation in Adults using Tooth Coronal Index Method-A Pilot Study
S Joparti, MJ Kiran, GV Rao, Y Sivaranjani, M Thakur, K Pradeepthi
Journal of Forensic Dental Sciences 13 (1), 38-43 2021



Age estimation using mandibular ramus and gonial angle using digital orthopantamogram
M Kathoju, VR Guttikonda
Int J Forensic Odontol 6 (1), 27-31 2021



Salivary malondialdehyde in oral submucous fibrosis–A marker for oxidative damage
SR Ganta, S Chittemsetti, T Sravya, VR Guttikonda
Journal of Oral and Maxillofacial Pathology 25 (1), 82-87 2021



Assessing the accuracy of Cameriere’s Indian-specific formula for age estimation on right and left sides of orthopantomogram
P Vadla, R Surekha, GV Rao, G Deepthi, S Naveen, CA Kumar
Egyptian Journal of Forensic Sciences 10, 1-6 2020



Stathmin! An immunohistochemical analysis of the novel marker in Oral Squamous Cell Carcinoma and Oral Leukoplakia
P Vadla, S Yeluri, G Deepthi, VR Guttikonda, S Taneeru, S Naramala
Asian Pacific Journal of Cancer Prevention: APJCP 21 (11), 3317 2020



Estimation of serum pyruvic acid levels in oral squamous cell carcinoma
P Guduguntla, VR Guttikonda
Journal of Oral and Maxillofacial Pathology 24 (3), 585 2020



Immunohistochemical expression of kallikrein 7 in oral squamous cell carcinoma
DV Kumar, Y Sivaranjani, GV Rao
Journal of Oral and Maxillofacial Pathology 24 (3), 580 2020



Recurrent adenoid cystic carcinoma of paranasal sinuses: a rare case report
SA Wahed, K Pradeepthi, MJ Kiran, GV Rao
Journal of Oral and Maxillofacial Pathology 24 (Suppl 1), S28-S32 2020



Sclerosing polycystic adenosis of hard palate: A rare entity in salivary glands
P Guduguntla, R Korlepara, VR Guttikonda
Contemporary Clinical Dentistry 10 (4), 676-678 2019



Analysis of collagen fibers in keratocystic odontogenic tumor and ameloblastoma: A polarizing microscopic study
K Peddapelli, V Rao, MP Kumar, T Sravya, D Rakesh
Indian Journal of Dental Research 30 (5), 731-735 2019



Odontogenic myxoma-A rare case report
K Mounika, S Ramulu, GV Rao, MP Kumar
Journal of Dr. NTR University of Health Sciences 8 (2), 118-121 2019



Rhinocerebral mucormycosis associated with actinomycosis in a diabetic patient: A rare presentation
SU Rani, Y Sivaranjani, MP Kumar, GV Rao
Journal of Oral and Maxillofacial Pathology 23 (Suppl 1), 122-125 2019



Solitary plasmacytoma of mandible: a rare entity
S Chittemsetti, VR Guttikonda, T Sravya, PK Manchikatla
Journal of Oral and Maxillofacial Pathology 23 (1), 136-139 2019



Estimation of serum sialic acid in oral submucous fibrosis and oral squamous cell carcinoma
S Chittemsetti, PK Manchikatla, V Guttikonda
Journal of Oral and Maxillofacial Pathology 23 (1), 156 2019



Demystifying the nature of hard tissues in odontogenic tumors using Modified Gallego's stain: A preliminary study
SN Afroze, S Ramulu, GV Rao, S Taneeru, R Bashamalla, P Vadla
Journal of Oral and Maxillofacial Pathology 22 (3), 448 2018



Nevoid basal cell carcinoma syndrome: A case report
VK Dodda, S Taneeru, VR Guttikonda, R Gaddipati
Journal of Dr. NTR University of Health Sciences 7 (3), 200-203 2018



Basaloid squamous cell carcinoma: Report of two rare cases and review of literature
K Peddapelli, GV Rao, T Sravya, S Ravipati
Journal of Oral and Maxillofacial Pathology 22 (2), 285 2018



Natural substitutes for formalin: A boon to histopathology!!
S Chittemsetti, S Nallamala, T Sravya, VR Guttikonda, PK Manchikatla, ...
Journal of Oral and Maxillofacial Pathology 22 (1), 143 2018



Ameloblastoma arising in the wall of dentigerous cyst: Report of a rare entity
SK Kondamari, S Taneeru, VR Guttikonda, GK Masabattula
Journal of oral and maxillofacial pathology 22 (Suppl 1), S7-S10 2018

MOST CITED SCHOLAR PUBLICATIONS

Mucormycosis in a diabetic patient: a case report with an insight into its pathophysiology
SN Afroze, R Korlepara, GV Rao, J Madala
Contemporary clinical dentistry 8 (4), 662-666 2017
Citations: 151



Sex determination using maxillary sinus
RK Kanthem, VR Guttikonda, S Yeluri, G Kumari
Journal of forensic dental sciences 7 (2), 163-167 2015
Citations: 122



Age and gender assessment through three-dimensional morphometric analysis of maxillary sinus using magnetic resonance imaging
SU Rani, GV Rao, DR Kumar, T Sravya, Y Sivaranjani, MP Kumar
Journal of forensic dental sciences 9 (1), 81-85 2017
Citations: 56



Glut-1 as a prognostic biomarker in oral squamous cell carcinoma
JM Harshani, S Yeluri, VR Guttikonda
Journal of oral and maxillofacial pathology 18 (3), 372-378 2014
Citations: 51



An increased risk of osteoporosis during acquired immunodeficiency syndrome
N Annapoorna, GV Rao, NS Reddy, P Rambabu, K Rao
International Journal of Medical Sciences 1 (3), 152 2004
Citations: 50



Ameloblastoma arising in the wall of dentigerous cyst: Report of a rare entity
SK Kondamari, S Taneeru, VR Guttikonda, GK Masabattula
Journal of oral and maxillofacial pathology 22 (Suppl 1), S7-S10 2018
Citations: 42



Estimation of hemoglobin, serum iron, total iron-binding capacity and serum ferritin levels in oral submucous fibrosis: a clinicopathological study
M Thakur, VR Guttikonda
Journal of Oral and Maxillofacial Pathology 21 (1), 30-35 2017
Citations: 31



Modified ultrafast Papanicolaou staining technique: A comparative study
M Thakur, VR Guttikonda
Journal of cytology 34 (3), 149-153 2017
Citations: 30



Studies on the extraction and characterization of thermostable a-amylase from pericarp of Borassus indica
MS Rao, NS Reddy, GV Rao, KRSS Rao
African Journal of Biotechnology 4 (3), 288-291 2005
Citations: 23



Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study
T Sravya, GV Rao, MG Kumari, YV Sagar, Y Sivaranjani, K Sudheerkanth
Journal of oral and Maxillofacial Pathology 22 (1), 148 2018
Citations: 21



Appendage deformity syndrome a nutritional disease of Macrobrachium rosenbergii
AR Kumar, GV Rao, KRSS Rao
Diseases of aquatic organisms 59 (1), 75-78 2004
Citations: 21



Assessing the accuracy of Cameriere’s Indian-specific formula for age estimation on right and left sides of orthopantomogram
P Vadla, R Surekha, GV Rao, G Deepthi, S Naveen, CA Kumar
Egyptian Journal of Forensic Sciences 10, 1-6 2020
Citations: 19



Solitary plasmacytoma of mandible: a rare entity
S Chittemsetti, VR Guttikonda, T Sravya, PK Manchikatla
Journal of Oral and Maxillofacial Pathology 23 (1), 136-139 2019
Citations: 19



Inflammatory myofibroblastic tumor of mandible: A rare case report and review of literature
R Korlepara, VR Guttikonda, J Madala, S Taneeru
Journal of Oral and Maxillofacial Pathology 21 (1), 136-139 2017
Citations: 17



Mesiodistal odontometrics as a distinguishing trait: A comparative preliminary study
T Sravya, RK Dumpala, VR Guttikonda, PK Manchikatla, VC Narasimha
Journal of Forensic Dental Sciences 8 (2), 99-102 2016
Citations: 17



Granular cell ameloblastoma of jaw–Report of a case with an emphasis on its characterization
S Taneeru, VR Guttikonda, S Yeluri, J Madala
Journal of Clinical and Experimental Dentistry 5 (3), e154 2013
Citations: 17



Rhinocerebral mucormycosis associated with actinomycosis in a diabetic patient: A rare presentation
SU Rani, Y Sivaranjani, MP Kumar, GV Rao
Journal of Oral and Maxillofacial Pathology 23 (Suppl 1), 122-125 2019
Citations: 16



Estimation of serum sialic acid in oral submucous fibrosis and oral squamous cell carcinoma
S Chittemsetti, PK Manchikatla, V Guttikonda
Journal of Oral and Maxillofacial Pathology 23 (1), 156 2019
Citations: 16



Age estimation using exfoliative cytology and radiovisiography: A comparative study
S Nallamala, VR Guttikonda, PK Manchikatla, S Taneeru
Journal of Forensic Dental Sciences 9 (3), 144-148 2017
Citations: 16



Unicystic ameloblastoma in conjunction with peripheral ameloblastoma: A unique case report presenting with diverse histological patterns
S Ghattamaneni, S Nallamala, VR Guttikonda
Journal of Oral and Maxillofacial Pathology 21 (2), 267-272 2017
Citations: 16