VELLIYUR KANNIAPPAN GOPALAKRISHNAN
@bhu.edu.et
PROFESSOR IN BIOCHEMISTRY, SCHOOL OF MEDICINE, BULE HORA UNIVERSITY INSTITUTE OF HEALTH
BULE HORA UNIVERSITY, ETHIOPIA
EDUCATION
Ph.D. Biochemistry
1996 University of Madras, Dr. ALMPGIBMS, Taramani Campus, Chennai.
Doctoral thesis title: Biochemical studies on the effect of cis-diammine dichloro platinum - II (cisplatin) and sodium selenite in experimental fibrosarcoma rats.
M. Phil., Biochemistry
1990 University of Madras, Dr. ALMPGIBMS, Taramani Campus, Chennai.
M.Phil thesis title: Mycotoxicoses, effect of patulin on plasma proteins. A preliminary study in rats.
M.Sc. Biochemistry
1988 University of Madras, Dr. ALMPGIBMS, Taramani Campus, Chennai.
B.Sc., Chemistry
1986 MSSWB College, Madurai Kamaraj University, Madurai.
RESEARCH INTERESTS
• Anticancer effect of Medicinal plants against prostate and ovarian cancer in vitro, in vivo and in silico model.
• Antidiabetic and anti-inflammatory activity of medicinal plants.
• Synthesis and Characterization of Nanoparticles from medicinal plants against cancer cell lines.
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Shine Kadaikunnan, P. Vijayaragavan, M.A. Rathi, Balasubramanian Balamuralikrishnan, Naiyf S. Alharbi, V.K. Gopalakrishnan, Sumitha Purushothaman, and Ravikumar Sivanesan
Elsevier BV
M. Valan Arasu, V. K. Gopalakrishnan, P. Vijayaragavan, M. A. Rathi, Naif Abdullah Al-Dhabi, Ki Choon Choi, and Gauri Singh
De Gruyter
Dugganaboyana Guru Kumar, Raghu Ram Achar, Jajur Ramanna Kumar, Ganamaedi Amala, Velliyur Kanniappan Gopalakrishnan, Sushma Pradeep, Ali A. Shati, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Ekaterina Silina,et al.
Springer Science and Business Media LLC
Abstract Background Viscum orientale is a largely used parasitic plant with traditional medicinal properties. They are considered to possess the medicinal properties of host tree which they grow on. It’s a least explored plant with ethanopharmacological importance. As a result, the current work aimed to investigate the biological effects of Viscum orientale extract and silver nanoparticles (AgNPs) generated from it. Methods AgNPs synthesized using Viscum orientale plant extract and analysed on time dependent series and was characterized using Ultra Violet UV–visible spectra, Fourier Transform Infrared Spectroscopy FTIR, X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX), Scanning Electron Microscopy (SEM). Further using disc method anti-microbial assay was performed following antioxidation screening using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), reducing power and nitric oxide content and heamgglutination with human blood. Results On green synthesis using silver, the phyto contituents of plant Viscum orientale effectively reduced silver ions at 3-4 h of continuous stirring to form AgNPs. UV–vis spectra showed a typical peak of AgNPs at 480 nm. The FTIR analysis confirmed the covering of silver layers to bio-compounds of the extract. SEM analysis represented AgNPs as spherical morphologies ranging from 119–222 nm. AgNPs exhibited impressive zone of inhibition against Escherichia coli (8.1 ± 0.3 mm), Staphylococcus aureus (10.3 ± 0.3 mm), Bacillus subtilis (7.3 ± 0.3 mm), Bacillus cereus (8.2 ± 0.3 mm), Salmonella typhi (7.1 ± 0.2 mm). AgNps exhibited efficiency against DPPH at EC50 value of 57.60 µg/ml. and reducing power at EC50 of 53.42 µg/ml and nitric oxide scavenging of EC50 of 56.01 µg/ml concentration. Further, anthelmintic activity results showed synthesized nanoparticles significant reduction in the paralysis time to 5.4 ± 0.3 min and death time to 6.5 ± 0.6 min in contrast to the individual factors. On hemagglutination using AgNPs, above 80 µg/ml of concentration showed very significant effect on comparison with water extract. Conclusion Synthesized AgNPs using Viscum orientale water extract displayed versatile biological activity than individual extract. This study has forecasted a new path to explore more on this AgNPs for further research.
P. Vijayaragavan, Sumitha Purushothaman, Selvaraj Arokiyaraj, M.A. Rathi, V.K. Gopalakrishnan, Mansour K. Gatasheh, and Ravikumar Sivanesan
Elsevier BV
M.Valan Arasu, P. Vijayaragavan, Sumitha Purushothaman, M.A. Rathi, Naif Abdullah Al-Dhabi, V.K. Gopalakrishnan, Ki Choon Choi, and S. Ilavenil
Elsevier BV
Guru Kumar Dugganaboyana, Chethan Kumar Mukunda, Anisha Jain, Raghavendra Mandya Kantharaju, Rani R. Nithya, Divya Ninganna, Rathi Muthaiyan Ahalliya, Ali A. Shati, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi,et al.
Frontiers Media SA
Introduction: The use of plant extracts in the green synthesis of metallic nanoparticles is one of the simplest, most practical, economical, and ecologically friendly methods for avoiding the use of toxic chemicals.Method: Silver nanoparticles (AgNPs) were synthesized, employing a high-efficiency, non- toxic, cost-effective, green, and simple technique that included the use of Salacia oblonga root extract (SOR) as a capping agent compared to synthetic nanoparticles. The use of S. oblonga can be seen in traditional medicines for treating diabetes, obesity, rheumatism, gonorrhea, asthma, and hyperglycemia. The objectives of the current study were to green synthesize S. oblonga root extract silver nanoparticles (SOR-AgNPs), characterize them, and study their antioxidant, antibacterial, and antidiabetic activities.Result: The shape of SOR-AgNPs was spherical, at less than 99.8 nm in size, and exhibited a crystalline peak at XRD. The green synthesized SOR-AgNPs showed significant antioxidant properties like DPPH (80.64 μg/mL), reducing power capacity (81.09 ± SEM μg/mL), nitric oxide (96.58 μg/mL), and hydroxyl (58.38 μg/mL) radical scavenging activities. The MIC of SOR-AgNPs was lower in gram-positive bacteria. The SOR-AgNPs have displayed efficient inhibitory activity against α-amylase, with an EC50 of 58.38 μg/mL. Analysis of capping protein around the SOR-AgNPs showed a molecular weight of 30 kDa.Discussion: These SOR-AgNPs could be used as antibacterial and antidiabetic drugs in the future as it is cheap, non-toxic, and environmentally friendly. Bio-fabricated AgNPs had a significant impact on bacterial strains and could be used as a starting point for future antibacterial drug development.
D. Sophia, V. K. Gopalakrishnan, C. Ram Kumar, and B. Vijayalakshmi
Springer International Publishing
R. Vijay, V.M. Drisya, Daniel Raja Femil Selta, M.A. Rathi, VK. Gopalakrishnan, Dalal Hussien M. Alkhalifah, and Wael N. Hozzein
Elsevier BV
Rami Adel Pashameah, Atif Abdulwahab A. Oyouni, Osama M. Al-Amer, Waseem AlZamzami, Hussam A.E. Althagafi, Fahad Alharthi, P. Vijayaraghavan, M.A. Rathi, V.K. Gopalakrishnan, and V. Duraipandiyan
Elsevier BV
P. Vijayaragavan, M.A. Rathi, V.K. Gopalakrishnan, Rami Adel Pashameah, Atif Abdulwahab A. Oyouni, Osama M. Al-Amer, Waseem AlZamzami, Hussam Awwadh E. Althagafi, V. Duraipandiyan, and Fahad Alharthi
Elsevier BV
Anusooriya Palanirajan, Poornima Kannappan, and Gopalakrishnan Velliyur Kanniappan
Elsevier BV
Muthaiyan Ahalliya Rathi, Elliangal Kunhappa Girisan, Velliyur Kanniappan Gopalakrishnan, Periasamy Meenakshi, and Dugganaboyana Guru Kumar
Springer Nature Singapore
Haftamu Tesfay Berihu, Tesfay Welderfael, Berihu Tekluu, V. K. Gopalakrishnan, M. Rama Rao, P. P. N. Vijay Kumar, U. Shameem, P. John Dogulas, and K. Krishna Chaithanya
Springer Science and Business Media LLC
Solomon Yeabyo, Zenebe Teka M, Gopalakrishnan V. K, Kamalakararao K, Muthulingam M, and Krishna Chaithanya K
A and V Publications
Objective: The present study was aimed to evaluate the in vivo immune protective potential of chloroform root extract of Sida schimperiana on E.coli 018:K1 induced peritonitis in albino Wistar rats. Methods: Acute toxicity of was performed by oral administration of S. schimperiana chloroform root extract (SSRCH) 5, 50, 300 and 2000mg/kg body of male albino Wistar and mortality was monitored for 14 days. Based on LD50, 1/10th, 1/5th cut-off values of the (SSRCH) plant extract was selected as a dose for E. coli induced peritonitis in albino Wistar rats. Wistar rats were pre-treated with 200 and 400mg/kg/bwt of SSRCH and Standard antibiotic Ofloxacin 5mg/Kg body weight was given oraly for a period of 7 days. The dosing regimens were started on day -1,-2, -3, 0, 1, 2 and 3 relative to the day of challenge (day 0) with 2×104 CFU of E. coli CFU/ml (i.p.) and mortality was monitored for 14 days. After the monitoring the mortality, the treated (Groups I-IV) rats were sacrificed, and assess the in vivo antibacterial activity of S. schimperiana chloroform root extract by determination of CFU/ml in peritoneal lavage fluid. Further SSRCH extract (400mg /kg bw) was analyzed by the neutrophil adhesion in Wistar rats for evaluated immunomodulatory activity. Results: In acute toxicity studies no mortality was observed for 24-48 hours. SSRCH extract 400mg (Group-IV) showed protection against E. coli induced peritonitis in albino Wistar rats by showing 60% survivability and also exhibited significantly increased percentage of neutrophils adhesion. i.e (25.74±2.351and 36.14±5.609) at 200 and 400mg/kg respectively. Conclusion: The present study concluded that the chloroform root extract of S. schimperiana had a significant amount of polyphenolic compounds and could serve as a potential source of natural antibacterial and Immunomodulatory agents for the development of therapeutic antibiotics with immunostimulatory activity in the treatment of intraperitoneal infections.
Tekleweyni Tadege, Kidu Hintsa, Tesfay Weletnsae, Gopalakrishnan V K, Muthulingam M, Kamalakararao K, and Krishna Chaithanya K
GP Innovations Pvt. Ltd.
Acacia etbaica Schweinf belongs to the family Fabaceae widely distributed in Africa and various parts of this plant such as bark, leaves, flowers and roots are widely used as a folk medicine for curing of various ailments.This study was aimed to screen the phytoconstituents and evaluate the antibacterial activity of leaf extract of A. etbaica against selected multidrug resistant Enterobacteriaceae family. Leaves of A. etbaica were extracted with petroleum ether, chloroform, acetone, and ethanol by sequential soxhlet extraction. Phytochemical screening of organic leaf extract of A.etbaica was carried out for the detection of phytoconstituents accountable for antibacterial activity. In vitro antibacterial activities of A.etbaica leaf extracts against selected Enterobacteriaceae family gram‑positive bacteria such as (B.subtilis, E.faecalis,S.aureus)and gram-negative (E. coli, K. pneumonia, V. cholera) were evaluated by agar well diffusion. The antibacterial potential of acetone and ethanol leaf extracts of A.etbaica were determined by 96 well plate broth dilution assay. Among the tested organic leaf extracts, both acetone and ethanolic leaf extract of A.etbaica showed a potentially broad spectrum of in vitro antibacterial activity against tested multiple drug resistant Enterobacteriaceae family gram-positive pathogens such as B.subtilis, E. faecalis , and S.aureus and gram-negative bacterial strains E. coli, K. Pneumonia and V. Cholera with significant MIC values. The significant antibacterial activity of both acetone and ethanolic leaf extracts of A.etbaica was due to the subsistence of secondary metabolites phytoconstituents such as flavonoids and phenolic compounds.
Kidu Hintsa, Tekleweyni Tadege, Tesfay Weletnsae, Gopalakrishnan V K, Kamalakararao K, and Krishna Chaithanya K
GP Innovations Pvt. Ltd.
Otostegia integrifolia Benth (O. integrifolia) is the endogenous medicinal plant of Ethiopia mostly used for the treatment of Stomach ache, tonsillitis, hypertension, malaria, ascariasis, and lung diseases. The current study was focused on phytochemical analysis and evaluation of the antibacterial activity of O. integrifolia Benth leave extracts against selected human bacterial pathogen by the agar well diffusion and microtube broth dilution method. Plant leaves were extracted with less polar to high polar solvents such as petroleum ether, chloroform, acetone, and ethanol. Phytochemical investigation was carried out for the identification of secondary metabolites responsible for antibacterial activity. In vitro antibacterial potential of O.integrifolia Benth leaf organic extracts against human pathogenic gram-negative (E. coli, K. pneumonia, V. cholera) and positive bacteria (B.subtilis, E. faecalis, S.aureus) were assessed by agar well diffusion, and bacterial inhibitory concentration of effective plant extracts was determined by 96 well plate broth dilution assay. Among all the tested organic leaf extracts, the acetone leaf extract of O.integrifolia Benth exhibited a promising broad spectrum of in vitro antibacterial activity against all tested multiple drug-resistant bacteria pathogens with significant MIC values of K.pneumoniae (2.144µg/ml), V. cholera (2.025µg), B.substilis (2.604 µg), and S.aureus (3.028 µg), respectively. The significant antibacterial activity of acetone leaf extracts of O. integrifolia Benth was due to the existence of flavonoids and phenolic compounds. The current studies demonstrated that the broad-spectrum antibacterial activity of acetone leaf extracts of O.integrifolia Benth might be helpful for the isolation of novel potent antibacterial agents against infectious bacterial pathogens without any side effects.
B. Vidya, M. Palaniswamy, J. Angayarkanni, K Ayub Nawaz, M. Thandeeswaran, K Krishna Chaithanya, Berihu Tekluu, Karthi Muthusamy, and V.K. Gopalakrishnan
Elsevier BV
Tesfay Weletnsae, Kidu Hintsa, Tekleweyni Tadege, V. K. Gopalakrishnan, Zenebe Hagos, Berihu Tekluu, and K. Krishna Chaithanya
A and V Publications
Cyclooxygenase-2 (COX-2) and 5- Lipoxygenase (5-LOX) are major enzymes involved in inflammatory pathways and plays a key role in the pathophysiology of various inflammatory diseases and several cancers. Cucumis ficifolius is a perennial herb belongs to the family name Cucurbitaceae, traditionally its roots have been used to treat abdominal cramp and the fresh fruit with an end cut off is applied for inflamed fingers. Based on the traditional importance the plant, it was selected for the screening of anti-inflammatory activity. The aims of the present study were to evaluate the in vitro anti-inflammatory activity of organic root extract of C. ficifolius by COX-2 and 5- LOX inhibitory activities by ELISA method. Qualitative phytochemical analysis of ethanolic root extract of Cucumis ficifolius revealed that the presence of flavonoids, phenolic compounds, tannins, saponins, quinones, and carbohydrates. Among all the tested extracts, the ethanolic root extract of C.ficifolius showed significant 5-LOX (60.14% at 100 µg/ml with IC50 values of 66.25µg/ml) and COX-2, selective inhibitory activity. (36.55% at 100 µg/ml with IC50 140.10 µg/ml. The present study concluded that the ethanolic root extract of C. ficifolius (CFRET) is a promising source for the isolation and development of 5-LOX and COX-2 dual inhibitory compound for management of inflammatory disease.
K. Krishna Chaithanya, V.K. Gopalakrishnan, Zenebe Hagos, K. Kamalakararao, Patricia Ponce Noyola, P. John Dogulas, and D. Govinda Rao
Informa UK Limited
Abstract Mesua ferrea L. is a medicinal plant belongs to the family Calophyllaceae, it is extensively used in folk medicine for treatment of chronic inflammatory diseases. The present study was aimed to isolate the anti-inflammatory compound(s) from M. ferrea L bark ethyl acetate extracts by using analytical techniques such as Column Chromatography, TLC, HPLC, FT-IR, 1H, 13C NMR and LC-MS against TNF-α and NO production in LPS induced RAW 264.7 cells. Among the eight fractions obtained M. ferrea L chloroform-ethyl acetate fraction 5 (MFCE-F5) showed significant inhibitory activities on nitric oxide and TNF-α production in LPS induced RAW 264.7 cells. This study results recommended that the isolated bioactive compound Mesuaferrin-A from M. ferrea L bark ethyl acetate extract may be useful in the treatment of inflammatory diseases. MS.
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GRANT DETAILS
Completed Projects
1. Co-investigator in Department of Science and Technology (DST), New Delhi, India, Funded Major Research Project on “Development of mechanism-based antidiabetic formulation, standardization from Indian Traditional Herbz viz., bioavailability, stability studies” Amount sanctioned for the period of three years. lakhs.
2. Principal Investigator in University Grants Commission (UGC) New Delhi, India, Funded Major Research Project on “Expression of transfected FHIT gene in ovarian cancer cell lines” Amount sanctioned for the period of three years (01.04.2007-31.03.2010) Rs-8,03, 600.00 F.No 32-607/2006(SR) dated 02March 2007
3. Principal Investigator in Indian Council of Medical and Research (ICMR) New Delhi, India, Funded Major Research Project on “Anticancer effect of Medicinal Plants on Prostate Cancer Progression Model in vitro” Amount sanctioned for the period of two years (15.05.2007 – 14.5.2009) dated 07.05.2007