Vasilichin Vladisval Aleksandrovich

@clinic.nrcii.ru

molecular immuno-genetics laboratory
National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia



           

https://researchid.co/vladislavvasilichin

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Genetics and Molecular Biology, Cell Biology, Cancer Research

6

Scopus Publications

Scopus Publications

  • A Novel Pot-Economy Approach to the Synthesis of Triantennary GalNAc-Oligonucleotide
    Artem Evgenievich Gusev, Vladimir Nikolaevich Ivanov, Nikolai Andreevich Dmitriev, Aleksandr Viktorovich Kholstov, Vladislav Aleksandrovich Vasilichin, Ilya Andreevich Kofiadi, and Musa Rakhimovich Khaitov
    MDPI AG
    N-Acetylgalactosamine (GalNAc) is an efficient and multifunctional delivery tool in the development and synthesis of chemically modified oligonucleotide therapeutics (conjugates). Such therapeutics demonstrate improved potency in vivo due to the selective and efficient delivery to hepatocytes in the liver via receptor-mediated endocytosis, which is what drives the high interest in this molecule. The ways to synthesize such structures are relatively new and have not been optimized in terms of the yields and stages both in lab and large-scale synthesis. Another significant criterion, especially in large-scale synthesis, is to match ecological norms and perform the synthesis in accordance with the Green Chemistry approach, i.e., to control and minimize the amounts of reagents and resources consumed and the waste generated. Here, we provide a robust and resource effective pot-economy method for the synthesis of triantennary GalNAc and GalNAc phosphoramidite/CPG optimized for laboratory scales.

  • Novel substituted 5-methyl-4-acylaminoisoxazoles as antimitotic agents: Evaluation of selectivity to LNCaP cancer cells
    Kirill S. Sadovnikov, Dmitry A. Vasilenko, Yulia A. Gracheva, Nikolay A. Zefirov, Eugene V. Radchenko, Vladimir A. Palyulin, Yuri K. Grishin, Vladislav A. Vasilichin, Alexander A. Shtil, Pavel N. Shevtsov,et al.
    Wiley
    AbstractA series of novel antimitotic agents was designed using the replacement of heterocyclic cores in two tubulin‐targeting lead molecules with the acylated 4‐aminoisoxazole moiety. Target compounds were synthesized via heterocyclization of β‐aryl‐substituted vinylketones by tert‐butyl nitrite in the presence of water as a key step. 4‐Methyl‐N‐[5‐methyl‐3‐(3,4,5‐trimethoxyphenyl)isoxazol‐4‐yl]benzamide (1aa) was found to stimulate partial depolymerization of microtubules of human lung carcinoma A549 cells at a high concentration of 100 µM and to totally inhibit cell growth (IC50 = 0.99 µM) and cell viability (IC50 = 0.271 µM) in the nanomolar to submicromolar concentration range. These data provide evidence of the multitarget profile of the cytotoxic action of compound 1aa. The SAR study demonstrated that the 3,4,5‐trimethoxyphenyl residue is the key structural parameter determining the efficiency both towards tubulin and other molecular targets. The cytotoxicity of 3‐methyl‐N‐[5‐methyl‐3‐(3,4,5‐trimethoxyphenyl)isoxazol‐4‐yl]benzamide (1ab) to the androgen‐sensitive human prostate adenocarcinoma cancer cell line LNCaP (IC50 = 0.301 µM) was approximately one order of magnitude higher than that to the conditionally normal cells lines WI‐26 VA4 (IC50 = 2.26 µM) and human umbilical vein endothelial cells (IC50 = 5.58 µM) and significantly higher than that to primary fibroblasts (IC50 > 75 µM).

  • Novel organotin complexes with phenol and imidazole moieties for optimized antitumor properties
    E.A. Nikitin, D.B. Shpakovsky, V. Yu Tyurin, A.A. Kazak, Yu A. Gracheva, V.A. Vasilichin, M.S. Pavlyukov, E.M. Mironova, V.E. Gontcharenko, K.A. Lyssenko,et al.
    Elsevier BV

  • Phenotypic and functional characteristic analysis of THP-1 cell line as a model of inflammation
    , E.K. Kokinos, D.O. Kuzmina, , O.A. Kuchur, , S.A. Tsymbal, , V.A. Vasilichin, ,et al.
    Geotar-Media Publishing Group

  • Synthesis of modified conformationally fixed tricarbocyanine dyes for conjugation with therapeutic agents
    Irina A. Doroshenko, Kamilla G. Aminulla, Viatcheslav N. Azev, Tatiana M. Kulinich, Vladislav A. Vasilichin, Alexander A. Shtil, and Tatyana A. Podrugina
    OOO Zhurnal "Mendeleevskie Soobshcheniya"

  • Effects of metal oxide nanoparticles on toll-like receptor mRNAs in human monocytes
    Vladislav A. Vasilichin, Sergey A. Tsymbal, Anna F. Fakhardo, Elizaveta I. Anastasova, Andrey S. Marchenko, Alexander A. Shtil, Vladimir V. Vinogradov, and Elena I. Koshel
    MDPI AG
    For the widespread application of nanotechnology in biomedicine, it is necessary to obtain information about their safety. A critical problem is presented by the host immune responses to nanomaterials. It is assumed that the innate immune system plays a crucial role in the interaction of nanomaterials with the host organism. However, there are only fragmented data on the activation of innate immune system factors, such as toll-like receptors (TLRs), by some nanoparticles (NPs). In this study, we investigated TLRs’ activation by clinically relevant and promising NPs, such as Fe3O4, TiO2, ZnO, CuO, Ag2O, and AlOOH. Cytotoxicity and effects on innate immunity factors were studied in THP-1(Tohoku Hospital Pediatrics-1) cell culture. NPs caused an increase of TLR-4 and -6 expression, which was comparable with the LPS-induced level. This suggests that the studied NPs can stimulate the innate immune system response inside the host. The data obtained should be taken into account in future research and to create safe-by-design biomedical nanomaterials.