Antioxidant Activity, LC-MS/MS Identification and In Silico Analysis of the Ethanol Extract of Beneng Taro Leaves (Xanthosoma undipes K. Koch) Grown in Three Different Locations Diana Widiastuti, Rudi Dwi Hariyanto, Siska Elisahbet Sinaga, Wira Eka Putra, Sustiprijatno, et al. Tropical Life Sciences Research, 2026 Xanthosoma undipes K. Koch (Beneng taro) leaves are rich in bioactive compounds with reported antioxidant and anticancer potential. This study evaluated the antioxidant activity of ethanol extracts from Beneng taro leaves cultivated in three locations, identified active compounds using liquid chromatography–tandem mass spectrometry (LC-MS/MS), and assessed their anticancer potential through in silico analysis. Extraction was performed with 96% ethanol, followed by phytochemical screening, quantification of total flavonoids and phenolics, and antioxidant evaluation using the DPPH assay. LC-MS/MS identified several bioactive phenolic and flavonoid compounds, including quercetin, isorhamnetin, hispidulin, oleocanthal and cyclovalone, as key contributors to antioxidant activity. Leaves from high-altitude Cisarua (TB-1) had the highest flavonoid (94.49 ± 1.61 mg QE/g) and phenolic (97.35 ± 1.74 mg GAE/g) contents, with the strongest antioxidant activity (IC₅₀ = 42.96 μg/mL). Drug-likeness screening indicated favourable pharmacokinetic properties for several compounds. Molecular docking revealed strong binding affinities of quercetin, isorhamnetin, hispidulin, cochliophilin A, cyclovalone and oleocanthal to Kirsten rat sarcoma viral oncogene homolog (KRAS), a key oncogenic protein regulating cell growth, division and mutation, suggesting potential anticancer effects. These findings indicate that cultivation at higher altitudes enhances bioactive compound levels and antioxidant potential. Future studies should isolate active compounds, validate their effects in vivo, optimise cultivation practices, and explore their development into functional foods or nutraceuticals.
Drug-Likeness Screening, Enrichment Analysis, Molecular Docking, and Dynamics Simulation of Bioactive Compounds from Red Ginger (Zingiber officinale var. rubrum) as Anti-Angiogenic Agents Targeting VEGFA for Potential Pancreatic Cancer Treatment Wira Eka Putra, Diana Widiastuti, Hary Isnanto, Sustiprijatno Sustiprijatno, Arief Hidayatullah, et al. Gazi University Journal of Science, 2026 Pancreatic cancer is one of the deadliest malignancies and a leading cause of cancer-related mortality worldwide. It is often diagnosed at advanced stages, characterized by angiogenesis, metastasis, and resistance to conventional therapies, making it particularly difficult to treat. Therefore, the discovery of safer and more effective therapeutic options is urgently needed. Zingiber officinale var. rubrum known as red ginger is a traditional medicinal plant recognized for its therapeutic properties, particularly its anticancer potential. This present work utilized an extensive in silico methodology to assess the antiangiogenic potential of red ginger bioactive compounds by targeting VEGFA, a key regulator of angiogenesis. Drug-likeness screening, toxicity prediction, permeability analysis, and biological activity profiling were used to identify lead compounds. Enrichment analysis revealed involvement in cancer-associated pathways such as PI3K-Akt, MAPK, Ras, and Rap1. Molecular docking and dynamics simulations showed that apigenin, (+)-catechin, and (-)-epicatechin bind effectively to VEGFA, with performance comparable to or better than the reference drug Sunitinib. Apigenin demonstrated the highest complex stability, followed by (+)-catechin and (-)-epicatechin. These findings highlight the therapeutic promise of red ginger compounds for pancreatic cancer treatment through VEGFA inhibition. Additional lab-based experiments are needed to validate their molecular mechanisms and clinical potential.
Fish Protein Hydrolysate from Lemuru (Sardinella lemuru) Scales as a Food Protein: Isolation, Characterization, Antibacterial and Antioxidant Activities Evi Susanti, Jannatul Firdaus, Safriza Lailatul Hikmah, Norazlinaliza Salim, Nuniek Herdyastuti, et al. Trends in Sciences, 2026 Fish protein hydrolysates (FPHs) have attracted significant interest owing to their robust nutritional composition, including glutamic acid, aspartic acid, leucine, lysine, and valine. The scales of lemuru (Sardinella lemuru) are currently underutilized. Therefore, in this research, FPHs from lemuru scales were produced by using papain through various stages including pretreatment of fish scales waste; optimization enzymatically of the FPHs production from waste scales of lemuru fish using papain includes the incubation time and the quantity of papain; characterization includes proximate analysis, FTIR, and SDS-PAGE; and determination of FPHs antibacterial and antioxidant activities. The results showed that the optimum FPHs production at 90 min incubation time with 0.705 U papain produced FPHs as much as 21.43%, with the molecular weight achieved less than 37 kDa. The proximate analysis of the prepared FPHs showed moisture, protein, fat and ash content of 16.85, 36.52, 0.298 and 46.34%, respectively. FTIR results showed that identical hydroxyl, carbonyl, and amide functional groups indicated the presence of peptide bonds. These findings suggest the complete enzymatic hydrolysis, and at the same time the presence of the functional groups supporting the bioactivity potencies of the hydrolysates which are involved in antibacterial and antioxidant activities. Interestingly, FPHs from lemuru scales with a concentration of 10,000 ppm could inhibit the growth of Escherichia coli bacteria indicating clear zone as much as 5.62 ± 0.19 mm and Staphylococcus aureus bacteria was 7.50 ± 0.14 mm. The IC50 value for inhibiting DPPH free radical oxidation was 60.441 µg/mL for the FPHs of lemuru scales powder. Thus, in conclusion, the FPHs from the lemuru scales has the potential to be developed as a raw material for food protein with antibacterial and antioxidant activities. HIGHLIGHTS FPHs were successfully produced from lemuru ( lemuru) scales using papain, achieving an optimal yield (21.43%) at 90 min of incubation and 0.705 U of papain enzyme. Proximate analysis showed high protein content (36.52%) and the presence of key functional groups (hydroxyl, carbonyl, amide) was confirmed by FTIR, supporting the potential use of lemuru scale-derived FPHs as a functional food ingredient. The resulting FPHs demonstrated antibacterial activity against coli (5.62 mm inhibition zone) and S. aureus (7.50 mm), and antioxidant activity with an IC50 of 60.441 µg/mL for DPPH radical inhibition. GRAPHICAL ABSTRACT
elucidating the anti-Metastatic Potency of Morinda citrifolia l. compounds via targeting MMP9: an integrative Network Pharmacology and Molecular Dynamics simulation WK Putra, A Hidayatullah, D Widiastuti, H Isnanto, MF Heika, et al. South African Journal of Chemistry, 2026 Metastasis is a major cause of mortality in breast cancer, making it a critical target in therapeutic strategies. Morinda citrifolia L., a tropical medicinal plant, has shown promise due to its bioactive compounds. This study investigates the anti-metastatic potential of M. citrifolia compounds by targeting matrix metalloproteinase-9 (MMP-9), a key protein involved in tumor invasion and metastasis. An integrative in silico approach was employed, including drug-likeness and toxicity screening, biological activity prediction, membrane permeability analysis, target protein identification, enrichment analysis, protein structure validation, molecular docking, and molecular dynamics simulations. Among the screened compounds, quercetin and obtusin exhibited strong binding affinity for MMP-9. We suggest that quercetin inhibits MMP-9 via zinc chelation and multi-point hydrogen bonding, while obtusin acts through cavity remodeling and potential allosteric modulation. Both compounds overlap with Marimastat's binding site, an established MMP-9 inhibitor, but engage the enzyme through distinct molecular interactions, highlighting their complementary mechanisms. Quercetin and obtusin from M. citrifolia inhibit MMP-9 through different yet complementary modes of action, suggesting their potential as anti-metastatic agents. These findings support further investigation through in vitro and in vivo studies to validate their therapeutic efficacy.
Modulation Effect of Muntingia calabura Leaves Extracts Supplementation in High Fat Diet-Administrated BALB/c Mouse Wira Eka Putra, Intan Nilatus Shofiyah, Adelia Riezka Rahim, Arief Hidayatullah, Muhaimin Rifa’i Iraqi Journal of Science, 2025 Hyperglycemia is recognized as the primary indicator of diabetes. The occurrence of Hyperglycemia results from a combination of contributing factors, such as dietary habits, exercise routines, and overall lifestyle choices. Accumulating evidence showed the hyperglycemia also causes inflammation, which changes the body's cellular and immune systems. Similarly, the acute hyperglycemia increases the levels of pro-inflammatory cytokines such as TNF-α and IL-6. The present study aimed to determine the effect of Muntingia calabura leaves extracts administration toward the immune system in hyperglycemia-induced high-fat diet in mouse model. Five experimental groups were applied in this study, including the control group, hyperglycemic group, and three groups treated with M. calabura leaves extracts in several doses such as 400, 700, and 2800 mg/kg BW. Flow cytometry analysis was performed to evaluate the inflammation markers such as IL-1β, IFN-ɣ, or TNF-α on the subset of macrophages and Th1 cells. Our findings indicate that the extracts did not enhance or elevate the production of IL-1β by the macrophage subgroup/population. Similarly, the pro-inflammatory cytokines such as IFN-ɣ and TNF-α were reduced on the CD4 after treated with the extracts. Therefore, this study demonstrated that M. calabura leaves extracts exerts suppressive activity to maintain the physiological level of immune system. To be highlighted, the immunosuppression action reduced several inflammatory markers, including macrophages and Th1 cells. Thus, the present study suggested that M. calabura leaves extracts possibly become alternative and complementary medicine against hyperglycemia.
Molecular Docking and Molecular Dynamics Simulation Study of Anti-Tuberculosis Drug Candidates from Plant-Derived Natural Products against InhA Protein Science and Technology Asia, 2024
Management practices and development opportunities for intensive KUB chicken farming in Bengkulu, Indonesia N Nurmeiliasari, H Kusnadi, J Firison, E Ramon, WE Putra, E Fauzi, ... AIP Conference Proceedings 3228 (1), 030021 , 2026 2026
Improving the performance of village-owned enterprises in Mukomuko Regency Bengkulu Province F Rosbarnawan, Alfayanti, Harwindah, Almidianto, R Wati, A Ishak, ... IOP Conference Series: Earth and Environmental Science 1471 (1), 012043 , 2025 2025
Artikel Review: Pembangunan Pedesaan Berkelanjutan WE Putra, MZ Yuliarso Jurnal Ilmu Pertanian Kelingi 4 (1), 1-10 , 2024 2024 Citations: 6
Community perceptions of village innovation development using the pentahelix approach in Central Bengkulu regency WE Putra, H Subagio, D Wamaer, V Siagian, A Ishak, E Fauzi, ... Technological Innovations in Tropical Livestock Development for … , 2024 2024
Social Changes in the Development of Beef Cattle in Oil Palm Plantation Areas: Case of Jayakarta Village, Central Bengkulu Regency. A Ishak, J Firison, E Ramon, Z Efendi, T Hidayat, E Fauzi, WE Putra, ... Sodality: Jurnal Sosiologi Pedesaan 12 (2) , 2024 2024 Citations: 2
VALUASI MANAJEMEN PENGEMBANGAN KERBAU DI DESA KEMBANG SERI, KABUPATEN BENGKULU TENGAH WE Putra, PS Asriani JURNAL PETERNAKAN SILAMPARI (JPS) ISSN: 2089-4791 3 (1), 43-54 , 2024 2024
Farmers’ responses to the use of rice agro-advisory service application in South Bengkulu Regency A Ishak, T Margono, YH Alfayanti, E Fauzi, WE Putra, Miswarti, Yahumri, ... IOP Conference Series: Earth and Environmental Science 1297 (1), 012004 , 2024 2024
Evaluation of nutrition status and fertilization recommendations for lowland paddy (Case study in Merigi Kelindang District, Bengkulu Central Regency) Yahumri, Miswarti, Afrizon, S Rosmanah, A Ishak, WE Putra, E Fauzi, ... IOP Conference Series: Earth and Environmental Science 1297 (1), 012005 , 2024 2024
Optimization Model of Sustainable Income of Sheep-Corn Farm Integration in Banten Province, Indonesia V Siagian, R Ayu, M Thoha, T Ermawati, S Setiawan, W Eka, I Putra, ... 2024
Kondisi Keberlanjutan Budidaya Padi Gogo pada Lahan Kering Masam di Provinsi Bengkulu WE Putra Buletin Agritek 4 (2), 1-9 , 2023 2023 Citations: 1
Tingkat Adopsi Varietas Unggul Baru di Sentra Produksi Padi Sawah Irigasi Bengkulu Selatan WE Putra, A Ishak, E Fauzi, M Miswarti, Y Yahumri, S Rosmanah, ... Prosiding Seminar Nasional Pembangunan dan Pendidikan Vokasi Pertanian 4 (1 … , 2023 2023
Mobile E-Commerce UMKM Sumbawa M Fauzi, S Esabella Buletin Ilmiah Informatika Teknologi 2 (1), 19-25 , 2023 2023 Citations: 1
Optimization model of maize production in various land use in Banten province V Siagian, W Eka Putra, Z Salim, Ragimun E3S Web of Conferences 444, 02036 , 2023 2023
Adaptive Soybean Cultivation Technology on Space Between Immature Rubber Plants S Rosmanah, M Miswarti, A Alfayanti, T Wahyuni, T Rahman, H Artanti, ... AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian 7 (1), 40-47 , 2023 2023
Knowledge improvement of agricultural extender through technical guidance of paddy soil test kit Y Yahumri, E Fauzi, WE Putra, A Ishak, A Alfayanti, M Miswarti, J Firison, ... E3S Web Of Conferences 373, 04028 , 2023 2023 Citations: 4
Farmers' Knowledge And Adoption Level Of Vub In Tebing Kaning Village, Bengkulu Utara Regency R Robiyanto, WE Putra, S Yuliasari, L Ivanti, Y Yahumri, T Wahyuni AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian 9 (2), 341-350 , 2022 2022
Produktivitas Padi di Lahan Rawa dengan Kapur Dolomit H Kusnadi, E Fauzi, A Ishak, J Firizon, WE Putra Jurnal Pertanian 13 (2), 47-53 , 2022 2022 Citations: 6
Weaking Manna Coconut Conservation Efforts In South Bengkulu District E Fauzi, M Miswarti, Y Yahumri, A Ishak, WE Putra, H Kusnadi, A Alfayanti, ... Iseprolocal , 2022 2022
Analysis Of Increasing Knowledge In The Making Of Local Micro Organisms (Mol) In Merigi Kelindang District Bengkulu Tengah M Miswarti, J Santoso, Y Octavia, WE Putra, A Afrizon, Y Yahumri, E Fauzi, ... AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 9 (1), 221-230 , 2022 2022 Citations: 3
Efficiency Analysis of Fried Onion Processing Business in Kwt Kemuning, Kepahiang Regency E Fauzi, S Sudarmansyah, E Kristanto, WE Putra, A Ishak SINTA Journal (Science, Technology, and Agricultural) 3 (1), 01-06 , 2022 2022
MOST CITED SCHOLAR PUBLICATIONS
Analisis keragaman plasma nutfah durian di Provinsi Bengkulu berdasarkan karakter morfologi M Miswarti, WE Putra, D Sugandi Buletin Plasma Nutfah 23 (1), 59-68 , 2017 2017 Citations: 10
Estimating model forecasting the price of chicken eggs in the city of Bengkulu HB Astuti, E Fauzi, WE Putra, A Alfayanti, A Ishak AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 8 (2), 137-147 , 2021 2021 Citations: 9
ANALISIS NILAI TAMBAH PENGOLAHAN GULA AREN (Kasus di Desa Gunung Kembang, Manna–Bengkulu Selatan) WE Putra, J Firison, L Harta, A Ishak Jurnal Agribis 13 (2) , 2020 2020 Citations: 9
Pendugaan Model Peramalan Harga Ekspor Kopi Indonesia F EMLAN, WE Putra, A Ishak, HB Astuti AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian 7 (1), 22-30 , 2020 2020 Citations: 9
Faktor-faktor yang mempengaruhi produksi padi sawah di kabupaten seluma E Makruf, Y Oktavia, WE Putra Balai Pengkajian Teknologi Pertanian (BPTP) Bengkulu , 2012 2012 Citations: 8
Faktor-Faktor Yang Mempengaruhi Produksi Padi Sawah Di Kabupaten Seluma (Studi Kasus: Produktivitas Padi Sawah di Desa Bukit Peninjauan II Kecamatan Sukaraja) E Makruf, Y Oktavia, WE Putra PROSIDING SEMINAR INOVASI TEKNOLOGI PERTANIAN SPESIFIK LOKASI MENDUKUNG … , 2012 2012 Citations: 7
Artikel Review: Pembangunan Pedesaan Berkelanjutan WE Putra, MZ Yuliarso Jurnal Ilmu Pertanian Kelingi 4 (1), 1-10 , 2024 2024 Citations: 6
Produktivitas Padi di Lahan Rawa dengan Kapur Dolomit H Kusnadi, E Fauzi, A Ishak, J Firizon, WE Putra Jurnal Pertanian 13 (2), 47-53 , 2022 2022 Citations: 6
Morphology characteristics of orchids species in Bukit Barisan, Bengkulu province Miswarti, I Calista, WE Putra, Y Oktavia, S Yuliasari, D Musaddad, ... IOP Conference Series: Earth and Environmental Science 653 (1), 012149 , 2021 2021 Citations: 5
Kontribusi Tenaga Kerja Dalam Keluarga Dan Pengaruhnya Terhadap Penerimaan Usahatani Sayuran Di Lahan Sawah Tadah Hujan WE Putra, E Fauzi, A Ishak AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 6 (2), 13-28 , 2019 2019 Citations: 5
Knowledge improvement of agricultural extender through technical guidance of paddy soil test kit Y Yahumri, E Fauzi, WE Putra, A Ishak, A Alfayanti, M Miswarti, J Firison, ... E3S Web Of Conferences 373, 04028 , 2023 2023 Citations: 4
Efektivitas Sosialisasi Terhadap Peningkatan Pengetahuan Petani Dalam Administrasi Kelompok Di Kecamatan Bunga Mas Kabupaten Bengkulu Selatan E Fauzi, MAR Ikhsan, J Firison, WE Putra, H Kusnadi, A Ishak Jurnal Citra Agri Tama 12 (2), 19-25 , 2022 2022 Citations: 4
PREFERENSI PETANI TERHADAP PEMBUATAN MIKRO ORGANISME LOKAL (MOL) DI DESA KELINDANG KABUPATEN BENGKULU TENGAH WE Putra, J Santoso, E Fauzi, J Firison, A Ishak JURNAL CITRA AGRI TAMA 12 (1), 1-7 , 2022 2022 Citations: 4
Financial analysis of RGL citrus ( Citrus reticulata ) farming in Rejang Lebong Regency Bengkulu E Fauzi, I Calista, Hamdan, WE Putra, W Mikasari, A Ishak, S Yuliasari, ... IOP Conference Series: Earth and Environmental Science 733 (1), 012095 , 2021 2021 Citations: 4
Analisis Usahatani Pola Tanam Sayuran Pada Lahan Gambut WE Putra, A Ishak, R Rokhani UNEJ e-Proceeding , 2019 2019 Citations: 4
Faktor-faktor yang mempengaruhi Produksi Padi Sawah di Kabupaten Seluma, Bengkulu E Makruf, Y Oktavia, WE Putra Balai Pengkajian Teknologi Pertanian Bengkulu , 2011 2011 Citations: 4
Analysis Of Increasing Knowledge In The Making Of Local Micro Organisms (Mol) In Merigi Kelindang District Bengkulu Tengah M Miswarti, J Santoso, Y Octavia, WE Putra, A Afrizon, Y Yahumri, E Fauzi, ... AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 9 (1), 221-230 , 2022 2022 Citations: 3
Respons Petani terhadap Dosis Pemupukan Spesifik Lokasi Padi Sawah Tadah Hujan (Kasus di Desa Ulak Lebar, Kecamatan Merigi Kelindang, Kabupaten Bengkulu Tengah) A Afrizon, DS Marbun, WE Putra, Y Yahumri, A Gaffar, E Fauzi, A Ishak Jurnal Inovasi Penelitian 3 (1), 4245-4248 , 2022 2022 Citations: 3
Efisiensi Pemanfaatan Lahan Sawah Irigasi dengan Tanaman Kangkung Cabut pada Kelompok Tani Panca Usaha di Kelurahan Rimbo Kedui Kabupaten Seluma–Bengkulu WE Putra, A Ishak, J Hendra Di dalam Prosiding Seminar Nasional Agroinovasi Spesifik Lokasi untuk … , 2017 2017 Citations: 3
Social Changes in the Development of Beef Cattle in Oil Palm Plantation Areas: Case of Jayakarta Village, Central Bengkulu Regency. A Ishak, J Firison, E Ramon, Z Efendi, T Hidayat, E Fauzi, WE Putra, ... Sodality: Jurnal Sosiologi Pedesaan 12 (2) , 2024 2024 Citations: 2
