Wawan Eka Putra

RESEARCH INTERESTS

Sistem Usaha Pertanian

Scopus Publications

Molecular Docking and Molecular Dynamics Simulation Study of Anti-Tuberculosis Drug Candidates from Plant-Derived Natural Products against InhA Protein




Computational study demonstrated anti-diabetic potencies of Diosgenin and Multiflorenol as peroxisome proliferator-activated receptor gamma agonist
Putra Wira Eka, Sustiprijatno, Arief Hidayatullah, Diana Widiastuti, and Muhammad Fikri Heikal
University of Saints Cyril and Methodius
The prevalence of diabetes mellitus continues to rise on a global basis, making this entity one of the most pressing issues facing public health nowadays. Generally, diabetes mellitus is characterized by increased blood sugar levels caused by insulin secretion or action abnormalities. Natural products have become more popular in treating various types of diseases, including diabetes mellitus, due to their minimal adverse effects. Promoting the peroxisome proliferator-activated receptor γ (PPARG) activation is an anti-diabetic strategy due to its biological function for adipocyte storage, mobilization, differentiation, and insulin sensitivity. This study aims to evaluate diosgenin and multiflorenol in silico as anti-diabetic drug candidates by targeting PPARG. Several analyses, such as molecular docking, protein target prediction, biological function prediction, protein-protein interaction, and pharmacokinetics analyses were carried out in this study. Computational prediction showed PPARG have involved in several activities, such as fat cell differentiation, fatty acid oxidation, fatty acid transport, and cellular response to fatty acid. The binding affinity score revealed that diosgenin and multiflorenol have a higher value than the control drug. Other characteristics, such as chemical interaction, amino acid residues, and physicochemical properties, demonstrated supportive drug development outcomes. Therefore, based on our findings, we suggested that diosgenin and multiflorenol, both of which target PPARG, would hold promise as potential candidates for an anti-diabetic drug.


The Effects of Water Extract of Psidium guajava L. Leaves Against the Percentage of Parasitemia in male Mus musculus L. Swiss Webster mice
Muh Ade Artasasta, Afifah Nurul Triyana, Dwi Listyorini, Wira Eka Putra, Hendra Susanto, and Rahmalia Ayunin
EDP Sciences
Due to increasing resistance, the accessibility of antimalarial medications is diminishing, necessitating the need for the development of novel drugs. Nevertheless, there is a dearth of knowledge regarding the examination of the malaria properties present in red guava leaves. This study aimed to investigate the impact of an water extract derived from red guava leaves (P. guajava L.) on the percentage of parasitemia in male mice (Mus musculus L. Swiss Webster). Additionally, the study sought to analyze the composition of phytochemical compounds in water extract obtained from red guava leaves. The findings demonstrated that the water extract of guava leaves (ADJB) and ART-ADJB (combination of artemisinin and ADJB), administered at doses of 100, 300, and 500 mg/kg, effectively decreased the percentage of parasitemia. ED50 value of red guava leaf water extract is 0.7741 mg/Kg BB. In combination with artemisinin, ED50 decreases to 0.7357 mg/Kg BB. Artemisinin alone has an ED50 of 0.43 mg/Kg BB. The measured ED50 value indicates that the utilization of a combination of artemisinin with red guava leaf water extract has greater potential compared to the use of red guava leaf water extract alone. The ADJB extract contains phytochemical compounds including flavonoids, alkaloids, and terpenoids.


Computational Screening of Rheum palmatum Phytochemicals as Potential Anti-Diabetes Agent via Sodium-Glucose Transport Protein 2 Inhibition




Ameliorative Effect of Jamaican Cherry (Muntingia calabura L.) Leaf Extract Toward Glucose Control and Immune Cells Modulation in High Fat Diet-Administrated Mice
Wira Eka Putra, Intan Nilatus Shofiyah, Adelia Riezka Rahim, Arief Hidayatullah, and Muhaimin Rifa’i
Yuzuncu Yil Universitesi Tarim Bilimleri Dergisi
Hyperglycemia is a dangerous condition in which too much glucose circulates in the blood plasma and is the leading cause of diabetes mellitus. It is a complex condition with varying degrees that can change over time, mainly owing to metabolic factors that reduce insulin secretion, decrease glucose use, and increase glucose production. This study aims to evaluate Muntingia calabura leaf extract's effect on glucose control and immune cell modulation in high-fat diet-administrated mice. According to the result, we found that M. calabura leaf extract significantly reduced the fasting blood sugar. Importantly, M. calabura leaf extract exerts immunomodulation effects by suppressing the relative number of regulatory T cells in the hypoglycemic mice model. Finally, this study showed M. calabura leaf extract exerts ameliorative potency against hyperglycemia by lowering the blood sugar level and suppressing the regulatory T cells. These results suggested that M. calabura leaf extract could develop into complementary and alternative medicine.


Immunosuppressive and Ameliorative Effects of Dietary Combined Herbs Extract of Curcuma zedoaria (Christm.) Roscoe and Phyllanthus niruri L. in DMBA-induced Breast Cancer Mouse Model
Wira Eka PUTRA
FABAD Farmasotik Bilimler Ankara Dernegi
Cancer still become the top health problem globally. Each year, there is an increased number of new incidence and mortality. A ton of modalities have been proposed as therapeutic strategy, including the application of medicinal plant-based therapy. In this study, we aimed to evaluate the immunomodulation effect of dietary combined herbs extract of Curcuma zedoaria and Phyllanthus niruri (cheral) in DMBA-induced breast cancer mouse model. About six groups of treatment were applied in experimental mice, including the administration of cheral extract with series of dose including 1.233 mg/kg BW, 2.466 mg/kg BW, and 4.932 mg/kg BW. Flow cytometry analysis was performed to evaluate immune parameters including NK cells, TNF-α and IFN-γ-expressing NK cells, CD4+TNF-α+IFN-γ+ type 1 helper T cells, and CD4+CD25+ regulatory T cells population. In this present study, we demonstrated that cheral extract exerts the immunosuppressive activity by attenuate the properties of immune system, such as NK cells, Th1 cells, and regulatory T cells in DMBA-induces mouse into normal level. Thus, we suggested that cheral extract has ameliorative potency as alternative or complementary therapy against cancer incidence.


Molecular Dynamics Simulation of Various Bioactive Compounds of Red Betel (Piper crocatum) as Anti-Inflammatory Drug-Like Candidates in Rheumatoid Arthritis Treatment
Siti Imroatul Maslikah and Wira Eka Putra
College of Graduate Studies, Walailak University
Joint inflammation and damage can be caused by rheumatoid arthritis (RA), a systemic autoimmune disorder. Long-term RA affects the joints, causing pain, edema, and stiffness. Several studies have revealed that the exact origin of RA is still unidentified, but inherited disorder and external factors may contribute to the inflammatory condition. The incidence of RA is mostly caused by uncontrolled-production of pro-inflammatory cytokines under nuclear factor-kappa B (NF-κB) overexpression condition. The nuclear factor-kappa B plays an important role in inflammatory T cell activation, differentiation, and effector function. Therefore, the inhibition of NF-κB can be a strategy to reduce the adverse effects or incidence of RA. Red betel is a perennial herb native to Indonesia that is widely used by conventional healers. Many reports show that red betel leaf extracts contain several critical bioactive compounds such as Apigenin, Luteolin, Hydroxychavicol, Eugenol, and Chavibetol. This present study aims to evaluate and predict the therapeutic potential of those compounds as medication that reduce inflammation against RA by disturbing and inactivating the NF-κB molecular interaction. The LC-MS chromatography was used to evaluate the chemical contents of red betel leaves. Furthermore, in silico study, the 3D structure of the active compounds of red betel leaves is obtained from PubChem web server, while NF-κB is obtained from Protein Data Bank (PDB) website. Molecular docking was used to screen the potential compounds compared to aspirin as control. As a result, 2 compounds including Apigenin and Luteolin have smaller bond affinity values, −6.3 and −6.0 kcal/mol, respectively compared to aspirin. Based on molecular dynamics (MD) simulation, the root-mean-square deviation (RMSD) value of protein-ligand complexes have the similar pattern over 1000/ps except the NF-κB-Chavibetol complex which has an unstable pattern. On the other hand, the total energy of the protein-ligand complexes have similar average number of −1.69 kJ/mol. The simulation indicates the ligands have constant binding energy to the protein. Furthermore, numerous pharmacokinetic properties of compounds including absorption, distribution, metabolism, excretion and toxicity are exponentially greater than the control drugs. Finally, red betel active compounds can be used as alternative candidates of RA therapy medication. HIGHLIGHTS Rheumatoid arthritis, a systemic autoimmune condition, damages joints. Long-term RA causes joint discomfort, swelling, and stiffness. The identified flavonoids from red betel leaf extract include flavonone, isoflavone, auron, catechin, anthocyanidin, and chalcone. Apigenin, chavibetol, eugenol, hydroxychavicol, and luteolin, the active chemicals found in red betel, have the potential to serve as NF-B inhibitors, making them potentially useful in the treatment of rheumatoid arthritis. GRAPHICAL ABSTRACT


Molecular Docking and ADMET Analysis of Bioactive Compounds from Aspergillus nomius NC06 Against Plasmepsin Protein: Antimalarial Activity




Determining the Molecular Interactions of Natural Inhibitors with the HPV-16 E6 Oncoprotein by Docking and Dynamics Simulation Studies




Computational Studies of Rhubarb’s Anthraquinone Glycosides as Anti-Diabetic Drug Candidates through Sodium-Glucose Cotransporter-2 Inhibition
Wira Eka Putra, Sustiprijatno, Arief Hidayatullah, Diana Widiastuti, and Muhammad Fikri Heikal
University of Baghdad College of Science
Type 2 diabetes mellitus continues to pose a significant global health concern, warranting increased attention. This metabolic disorder is influenced by various factors, such as lifestyle, diet, environment, and genetics. While several approaches have been developed to address the incidence of type 2 diabetes mellitus, current treatments remain inadequate and necessitate further improvement. In this study, our objective was to assess the potential antidiabetic efficacy of anthraquinone glycosides present in rhubarb (specifically physcion diglucoside and aloe-emodin-8-glucoside) as potential inhibitors of the sodium-glucose cotransporter-2. We employed an in silico study to evaluate their inhibitory properties. The computational prediction was performed included the target protein, ligands retrieval, and preparation. Furthermore, the molecular docking process was conducted to assess the interaction between the target protein and ligands. Subsequently, data visualization and analysis were performed. Various indicators were evaluated, including the characteristics of the target molecule and drug candidates, binding affinity scores, interaction positions, types of chemical interactions, amino acid residues involved, hydrophobicity, formation of hydrogen bonds, interpolated charge, and ionizability. These comprehensive evaluations provided valuable insights into the molecular interactions and potential efficacy of the tested compounds. In this computational study, we demonstrated that the both rhubarb anthraquinones, physcion diglucoside and aloe-emodin-8-glucoside, have a great potential as antidiabetic agent by inhibiting the action of the sodium-glucose cotransporter-2. Thus, the inactive the sodium-glucose cotransporter-2 can prevent the excessive number of glucose plasma level in type two diabetes mellitus people.


Determining the effect of benzo[a]pyrene exposures toward innate and adaptive immunity profiles in post-measles vaccination mouse model
Wira Eka Putra, Fa'urinda Riam Prabu Nery, Mochammad Imam Fauzi, and Muhaimin Rifa'i
Acta Pharmaceutica Sciencia
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A Molecular Docking and Dynamics Simulation Study on Prevention of Merozoite Red Blood Cell Invasion by Targeting Plasmodium vivax Duffy Binding Protein with Zingiberaceae Bioactive Compounds
Muhammad Fikri Heikal, Wira Eka Putra, Sustiprijatno, Arief Hidayatullah, Diana Widiastuti, and Mardiana Lelitawati
Universidad Nacional de Costa Rica
[Objective] Plasmodium vivax predominantly infects many people in numerous tropical areas, including Southeast Asia, the Western Pacific, the Americas, and the Eastern Mediterranean. The uniqueness of forming dormant stages can lead to relapse in vivax malaria upon further infection. This study used molecular docking and dynamic simulation to predict potential bioactive compounds from the Zingiberaceae plant family as inhibitors by targeting Plasmodium vivax Duffy Binding Protein (PvDBP). PvDBP-DARC molecular interaction is required to mediate the merozoite invasion process into red blood cells. Inhibiting this process can possibly control parasite growth and development. [Methodology] Molecular docking screening was conducted by using 138 natural compounds from the Zingiberaceae plant family targeting Plasmodium vivax Duffy binding protein (PvDBP). The top two compounds with the lowest binding energy were selected to be analyzed by pharmacokinetics prediction and molecular dynamic (MD) simulation. [Results] Molecular docking screening resulted in the top two compounds with the lowest binding energy value, including 5,7-dihydroxyflavanone (-9.3 kcal/mol) and pinostrobin (-9.2 kcal/mol). These compounds are predicted to have stronger interaction than chloroquine as a control. Furthermore, the potential compounds also interact with DARC binding site residues and maintain them during the molecular dynamic simulation process. Otherwise, chloroquine as a control cannot retain 75% binding residues towards PvDBP. A molecular dynamic study revealed that all three complexes have relatively similar stability. [Conclusions] We predicted that the two bioactive compounds (5,7-dihydroxyflavanone and pinostrobin) have the potential as merozoite invasion inhibitors.


Targeting the Coronavirus SARS‑CoV‑2's Envelope, Nucleocapsid, and Spike/ Spike RBD Protein: Computational Insight from Multiple Bioactive Compounds as Potential Anti-Viral Drug Candidates
Wira Eka Putra, , Muhammad Fikri Heikal, Sustiprijatno Sustiprijatno, Arief Hidayatullah, Diana Widiastuti, , , , and
University of Science and Technology of Southern Philippines
Global health, social, and economic systems have been seriously threatened by the coronavirus disease (COVID-19) pandemic. In addition to the increasing number of deaths, thousands of COVID-19 survivors continue to experience life-altering illness. This study aimed to evaluate multiple bioactive compounds from various indigenous medicinal plants against the structural proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including the envelope, nucleocapsid, and spike/spike receptor-binding domain (RBD) proteins, in search of potential antiviral drug candidates. Computational analysis was used to screen for binding affinities and assess chemical interactions between ligands and target proteins. The findings revealed the top three potential compounds to bind to the envelope protein (cafestol, kahweol, and ledene), nucleocapsid protein (cafestol, kahweol, and thearubigin), spike protein (tannic acid, eugeniin, and kahweol), and spike RBD protein (kahweol, cafestol, and tannic acid). Moreover, the study identified four types of plants that contain potential bioactive compounds against SARS-CoV-2 structural proteins, including black tea (Camellia sinensis), clove (Syzygium aromaticum), common bean (Phaseolus vulgaris), and star anise (Illicium verum). Interestingly, kahweol exhibited possible binding activity against all four target proteins. This result suggests that bioactive compounds from the listed medicinal plants could potentially be developed into antiviral drugs against COVID-19.


Antidiabetic Activity of Averrhoa bilimbi L. Fruit Extracts and the Identification of Active Compounds Using LC-MS and In silico Methods
Diana Widiastuti, Siska Elisahbet Sinaga, Siti Warnasih, Yulian Syahputri, Nurjanah Bella Saputri, Sustiprijatno, and Wira Eka Putra
Universitas Gadjah Mada
Patients with diabetes mellitus require drugs that do not cause side effects in long-term use. In this era, many people use herbal medicine to treat diabetes mellitus. One species we can use as herbal medicine is Averrhoa bilimbi L., a plant whose fruit is known as belimbing wuluh and is used because it has value as an alternative medicine. This study aims to determine the antidiabetic activity of the active fraction of Averrhoa bilimbi L. fruit in vitro and in silico and to obtain the active compounds contained in the fruit fraction of starfruit (A. bilimbi L.) using Liquid Chromatography Mass Spectrometer (LC-MS). Sixteen organic compounds were identified using LC-MS analysis of the n-hexane fraction, which had the highest IC50 (7.03 μg/mL) antidiabetic activity compared to ethyl acetate (102.77 μg/mL) and n-butanol (181.65 μg/mL). In silico molecular docking, analysis was performed for all identified compounds to support the antidiabetic characteristics further. Computational predictions in this investigation revealed that two A. bilimbi fruit compounds with the greatest RT value, namely 2,3,4,5,6-pentaphenylbenzazocine (-16.0 kcal/mol) and Thraustochytroside A (-6.2 kcal/mol), exhibited higher binding affinity values than miglitol (-5.4 kcal /mol). This indicates that both compounds have promising futures as α-glucosidase inhibitor alternatives for treating diabetic disorders.


Assessing the Immunomodulatory and Hepatoprotective Activities of Aqueous Tuber Extract of Typhonium flagelliforme (Lood) Blume in BALB/c Mouse
Hashemite University



Immunomodulatory Properties of Citrus limon Extracts on BALB/c Mouse Lymphoid and Myeloid Lineage Cells
Hashemite University



Identification of Active Compounds from Averrhoa bilimbi L. (Belimbing Wuluh) Flower using LC-MS and Antidiabetic Activity Test using in vitro and in silico Approaches
Diana Widiastuti, Siska Elisahbet Sinaga, Siti Warnasih, Erna Pujiyawati, Supriatno Salam, and Wira Eka Putra
College of Graduate Studies, Walailak University
Averrhoa bilimbi L., (Belimbing wuluh), is traditionally used for various antidiabetic, anti-larvicidal, antioxidant, hypoallergenic, antibacterial, antifungal, and anti-inflammatory treatments. This study evaluated 3 extracts (n-hexane, ethyl acetate, and n-butanol) isolated from starfruit flowers for antidiabetic effects using in vitro and in silico methods. Ethyl acetate extract showed the highest activity, followed by n-hexane and n-butanol extracts in inhibiting α-glucosidase. Each extract has an IC50 value of 89.84, 52.88, and 114.86 µg/mL. The active compounds contained in the ethyl acetate extract were determined using LC-MS, and the compounds with the highest retention time were identified as tautomycetin (8.585) and lithocholic acid (8.993). Tautomycetin and lithocholic acid showed promising results in the in silico test. The computational predictions showed that tautomycetin (−6.2 kcal/mol) and lithocholic acid (−10.0 kcal/mol) have better binding affinity values than miglitol (−5.4 kcal/mol). This is the first time the antidiabetic test of starfruit flower extract has been carried out. HIGHLIGHTS Averrhoa bilimbi L well-known as belimbing wuluh. is a highly nutritious plant and an endemic and tropical species mainly used as a traditional medicine to treat several diseases. Its fruit is food with economic value in Indonesia Extracts of ethyl acetate from the flower showed the highest antidiabetic activity with IC50 52.88 μg/mL In the phytochemical determination from Averrhoa bilimbi L flower using Liquid Chromatography Mass Spectrometer (LC-MS), several compounds were suspected of having antidiabetic activity, namely the compound lithocholic acid, and tautomycetin Lithocholic acid and tautomycetin have been tested for their bioactivity as antidiabetic with in vitro and in vivo methods GRAPHICAL ABSTRACT


Phytochemical Profiling and Pharmaceutical Properties of Moringa oleifera Leaves Powder and Seed Oil Against Hepatocellular Carcinoma
Hendra Susanto, Surjani Wonorahardjo, Wira Eka Putra, Ahmad Taufiq, Sunaryono Sunaryono, Dianvita Nur Fadhilah, Siti Bachrotus Recha Nur Fa’ida, Sa’diyatul Rizqie Amaliyah Firdaus, Moch. Sholeh, and Nik Ahmad Nizam Nik Malek
Penerbit UTM Press
Hepatocellular carcinoma (HCC) is one of the deadliest types of cancer with a mortality rate of 8.9% of the total cancer deaths in Indonesia. This cancer can be caused by exposure to hepatitis B and C viruses, NAFLD, autoimmune, diabetes to sporadic genetic diseases. The development of chronic HCC is generally preceded by the occurrence of severe liver fibrosis and cirrhosis. One of the genes that play a role in fibrosis in the incidence of HCC is TGF-β1. As a pro-fibrotic cytokine, the presence of high levels of TGF-β1 may be due to oxidative stress activity early in cancer development. One of the natural ingredients with lots of phytochemical content in the form of antioxidants that can reduce this activity is Moringa plant (Moringa oleifera). In this study we used a computational approach using molecular docking on the results of the GC-MS and LC-HRMS tests on Moringa oleifera Seed Oil (MOSEIL) and Moringa oleifera Leaves Powder (MOLP) which are oil and flour products made from moringa. The results of the identification of phytochemical compounds through the GC-MS test showed that the dominant compound in MOSEIL was oleic acid (37.546%) and in MOLP was ester (8.802%) when using n-hexane as solvent. The percentage yield of the dominant compound from the LC-HRMS test in MOSEIL was nitro compound (72.55%) and at MOLP was alcohol (45.87%). These compounds are known to have effects as hepatoprotective agents through antioxidant, anti-inflammatory, and anti-fibrotic activities that can reduce hepatic oxidative stress as an early trigger of cancer development. Through molecular docking, MOSEIL and MOLP showed a lower level of binding affinity when compared to TGF-β1 control drugs such as metformin. This data implies MOSEIL and MOLP have a strong interaction to TGF-β1 than the control drug. The therapeutic potential of the hepatoprotective properties of MOSEIL and MOLP makes them one of the most-promising therapeutic agents in the initial step of renewable cancer treatment therapy.


IN SILICO INVESTIGATION OF MUNG BEAN (Vigna radiata L.) ACTIVE COMPOUNDS AS POTENTIAL NATURAL INHIBITOR AGAINST HEPATITIS C VIRUS E2 ENVELOPE GLYCOPROTEIN




Molecular Docking and Molecular Dynamics Simulation-Based Identification of Natural Inhibitors against Druggable Human Papilloma Virus Type 16 Target
Arief Hidayatullah, Wira Eka Putra, Sustiprijatno Sustiprijatno, Diana Widiastuti, Wa Ode Salma, and Muhammad Fikri Heikal
College of Graduate Studies, Walailak University
The E5 protein is the smallest known oncoprotein linked to HPV 16 cancer development. In this study, we determined the potential of asarinin and thiazolo as an inhibitor of the E5 protein through molecular dynamics. The results showed that the binding site is unstable because of its hydrophobic nature and small size, causing considerable changes in the binding site for each of the 3 drugs examined. Except for asarinin, which still interacts with the first hydrophobic domain, they preserved their capacity to prevent endosomal acidification, hyper amplification of the EGFR pathway and contact with BAP31. It may inhibit E5-MHC I interaction. Thiazolo[3,2-a]benzymidazole-3(2H)-one,2-(2-fluorobenzylideno)-7,8-dimethyl (thiazolo) is expected to form more stable protein-ligand complexes than the other 2. However, the SASA, hydrogen bond and DCCM plots show that both compounds are equivalent to HPV 16 E5 protein. HIGHLIGHTS The smallest known oncoprotein associated with cancer development as a result of HPV 16 infection is the E5 protein The SASA, hydrogen bond, and DCCM plots, on the other hand, show that asarinin and thiazolo are equivalent to the HPV 16 E5 protein Thiazolo is predicted to produce more stable protein-ligand complexes than the others because of its unique molecular dynamic properties GRAPHICAL ABSTRACT


The Early Detection of Diabetes Mellitus in Younger Female Obese: A Pilot Project for GAD65 Kit in Students Community
Hendra Susanto, Lutfiyah Magfiroh Azis, Aulanni'am Aulanni'am, Dyah Kinasih Wuragil, Wira Eka Putra, Fatma Yuni Reformawati, Moch. Sholeh, and Sa'diyatul Rizqie Amaliyah Firdaus
AIP Publishing



The Computational Analysis of Sterol Moringa Seed Oil (MOSEIL) as Anti-Inflammatory Agent Against Type Diabetes Mellitus Complication
Wulan Dwi Saputri, Betty Lukiati, Hendra Susanto, Wira Eka Putra, Dianvita Nur Fadhilah, Siti Bachrotus Recha Nur Fa’ida, Farida Ariyani, and Alfi Nur Faizah
AIP Publishing



Profiling the Coulomb Energy of Chimanine D and Desulphosinigrin as Potential Anti-Diabetic Drug against Alpha-Glucosidase
Anissa Nofita Sari, Wira Eka Putra, Muhaimin Rifa'i, Sustiprijatno, Hendra Susanto, Wa Ode Salma, Muhammad Faisal, Arief Hidayatullah, Muhammad Fikri Heikal, and Sa'diyatul Rizqie Amaliyah Firdaus
AIP Publishing



The Expression of Metastasis-Related Protein Among Indonesian Colorectal Carcinoma Patients: A Preliminary Clinical Study
Hendra Susanto, Adeodatus Yuda Handaya, Wira Eka Putra, Nadilah Nur Anggraeni, Bay Ummu Sakinatuz Zakiah, Fitriana Hadayani, Moch. Sholeh, Sa'diyatul Rizqie Amaliyah Firdauz, Arum Linangkung, Rianto Prabowo,et al.
AIP Publishing



The Nutritional Supplementation of MOLP Water Extract in Enhancing Amaranthus sp plant Growth: A Pilot Study for Green Fertilizer
Sindora Dwi Pratiwi, Hendra Susanto, Wira Eka Putra, Ahmad Taufiq, Sunaryono Sunaryono, Ahmad Imam Mawardi, Moch. Sholeh, Ari Gunawan, and Muhammad Ashar
AIP Publishing

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Coconut manna: Conservation effort and its obtacles in South Bengkulu District, Indonesia
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Optimization Model of Sustainable Income of Sheep-Corn Farm Integration in Banten Province, Indonesia
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Optimization model of maize production in various land use in Banten province
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Iseprolocal 2022



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MOST CITED SCHOLAR PUBLICATIONS

ANALISIS NILAI TAMBAH PENGOLAHAN GULA AREN (Kasus di Desa Gunung Kembang, Manna–Bengkulu Selatan)
WE Putra, J Firison, L Harta, A Ishak
Jurnal Agribis 13 (2), 1-5 2020
Citations: 9



Analisis keragaman plasma nutfah durian di Provinsi Bengkulu berdasarkan karakter morfologi
M Miswarti, WE Putra, D Sugandi
Buletin Plasma Nutfah 23 (1), 59-68 2017
Citations: 9



Faktor-Faktor Yang Mempengaruhi Produksi Padi Sawah Di Kabupaten Seluma (Studi Kasus: Produktivitas Padi Sawah di Desa Bukit Peninjauan II Kecamatan Sukaraja)
E Makruf, Y Oktavia, WE Putra
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Pendugaan Model Peramalan Harga Ekspor Kopi Indonesia
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AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian 7 (1), 22-30 2020
Citations: 6



Faktor-faktor Yang Mempengaruhi Produksi Padi Sawah di Kabupaten Seluma
E Makruf, Y Oktavia, WE Putra
BPTP Bengkulu 2012
Citations: 6



Analisis Usahatani Pola Tanam Sayuran Pada Lahan Gambut
WE Putra, A Ishak, R Rokhani
UNEJ e-Proceeding 2019
Citations: 5



Knowledge improvement of agricultural extender through technical guidance of paddy soil test kit
Y Yahumri, E Fauzi, WE Putra, A Ishak, A Alfayanti, M Miswarti, J Firison, ...
E3S Web Of Conferences 373, 04028 2023
Citations: 4



Faktor-faktor yang mempengaruhi Produksi Padi Sawah di Kabupaten Seluma, Bengkulu
E Makruf, Y Oktavia, WE Putra
Balai Pengkajian Teknologi Pertanian (BPTP) Bengkulu 2011
Citations: 4



Estimating Model Forecasting the Price of Chicken Eggs In the City of Bengkulu
HB Astuti, E Fauzi, WE Putra, A Alfayanti, A Ishak
AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 8 (2), 137-147 2021
Citations: 3



Morphology characteristics of orchids species in Bukit Barisan, Bengkulu province
I Calista, WE Putra, Y Oktavia, S Yuliasari, D Musaddad, Y Sastro
IOP Conference Series: Earth and Environmental Science 653 (1), 012149 2021
Citations: 3



Kontribusi penerimaan usahatani kacang panjang di lahan sawah tadah hujan pada berbagai pola tanam (Kasus di Kecamatan Pondok Kelapa, Kabupaten Bengkulu Tengah)
WE Putra, S Sudarmansyah, A Ishak
AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 7 (1), 1-12 2020
Citations: 3



Kontribusi Tenaga Kerja Dalam Keluarga Dan Pengaruhnya Terhadap Penerimaan Usahatani Sayuran Di Lahan Sawah Tadah Hujan
WE Putra, E Fauzi, A Ishak
AGRITEPA: Jurnal Ilmu Dan Teknologi Pertanian 6 (2), 13-28 2019
Citations: 3



Efisiensi Pemanfaatan Lahan Sawah Irigasi dengan Tanaman Kangkung Cabut pada Kelompok Tani Panca Usaha di Kelurahan Rimbo Kedui Kabupaten Seluma–Bengkulu
WE Putra, A Ishak, J Hendra
Di dalam Prosiding Seminar Nasional Agroinovasi Spesifik Lokasi untuk 2017
Citations: 3



Kondisi Keberlanjutan Budidaya Padi Gogo pada Lahan Kering Masam di Provinsi Bengkulu
WE Putra
Buletin Agritek 4 (2), 1-9 2023
Citations: 2



Produktivitas Padi di Lahan Rawa dengan Kapur Dolomit
H Kusnadi, E Fauzi, A Ishak, J Firizon, WE Putra
Jurnal Pertanian 13 (2), 47-53 2022
Citations: 2



Respons Petani terhadap Dosis Pemupukan Spesifik Lokasi Padi Sawah Tadah Hujan (Kasus di Desa Ulak Lebar, Kecamatan Merigi Kelindang, Kabupaten Bengkulu Tengah)
A Afrizon, DS Marbun, WE Putra, Y Yahumri, A Gaffar, E Fauzi, A Ishak
Jurnal Inovasi Penelitian 3 (1), 4245-4248 2022
Citations: 2



Efektivitas Sosialisasi Terhadap Peningkatan Pengetahuan Petani Dalam Administrasi Kelompok Di Kecamatan Bunga Mas Kabupaten Bengkulu Selatan
E Fauzi, MAR Ikhsan, J Firison, WE Putra, H Kusnadi, A Ishak
Jurnal Citra Agri Tama 12 (2), 19-25 2022
Citations: 2



PREFERENSI PETANI TERHADAP PEMBUATAN MIKRO ORGANISME LOKAL (MOL) DI DESA KELINDANG KABUPATEN BENGKULU TENGAH
WE Putra, J Santoso, E Fauzi, J Firison, A Ishak
JURNAL CITRA AGRI TAMA 12 (1), 1-7 2022
Citations: 2



Financial analysis of RGL citrus (Citrus reticulata) farming in Rejang Lebong Regency Bengkulu
E Fauzi, I Calista, WE Putra, W Mikasari, A Ishak, S Yuliasari, Y Sastro
IOP Conference Series: Earth and Environmental Science 733 (1), 012095 2021
Citations: 2



Pengaruh Faktor-Faktor Produksi Terhadap Produktivitas Padi Sawah Di Kota Bengkulu (Studi Kasus Di Kelurahan Semarang, Kecamatan Sungai Serut)
WE Putra, A Ishak
2015
Citations: 2