WILLY BUSTILLOS TORREZ
@unifranz.edu.bo
RESEARCH
UNIFRANZ
As a dental student, I always liked teaching, which is why I was a teaching assistant for a large part of my career in the subjects of Biochemistry, Pediatric Dentistry, Orthodontics and Integral Children. After several years I decided to take on a personal challenge which is to pursue a postgraduate degree in Microbiology, the same one that did not exist in Bolivia, so I searched and after being evaluated I was admitted to 2 universities in Chile, of which I chose the one with the best ranking for its production in research, there I realized that I could also contribute in this area and I returned to the country to replicate what I had learned; however, I had the need to further train myself and achieve the highest postgraduate training, which is a doctorate, for that reason I applied to Brazil and thank you I was the first Bolivian to receive a scholarship from this organization at LAOHA, today I lead the International Association for Dental Research (IADR) Bolivia Section, which has
EDUCATION
UMSA BOL
UNIVERSITY OF TALCA CH
UNIVERSITY OG GUARULHOS BRA
RESEARCH, TEACHING, or OTHER INTERESTS
Microbiology, Dentistry, Multidisciplinary, Epidemiology
FUTURE PROJECTS
THIS MUTANT AND BACILON NOT MORE CARIES PLEASE
Applications Invited
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Willy Bustillos Torrez, Luciene Cristina Figueiredo, Thalita Dias Silva Santos, Geisla Mary Soares, João Marcos Spessoto Pingueiro, Hélio Doyle Pereira da Silva, Zilson Malheiros, Bernal Stewart, Magda Feres, and Bruno Bueno-Silva
Informa UK Limited
Abstract This study evaluated the effect of a mouthwash containing 0.075% cetylpyridinium chloride and 0.28% zinc lactate (CPC + Zn) in a multispecies biofilm model. A 7-days 33-species biofilm, formed on Calgary device, was 1-min treated with: 0.12% chlorhexidine (CHX), culture medium (negative control), 0.075% cetylpyridinium chloride (CPC) or CPC + Zn, 2x/day, from day 3 until day 6. The metabolic activity and the microbial composition were evaluated by colorimetric method and checkerboard DNA-DNA hybridization, respectively. The three antimicrobials (CPC, CPC + Zn and CHX) reduced metabolic activity, total biofilm count and several species counts, including Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra, Campylobacter gracilis and Streptococcus mutans. However, only CPC + Zn reduced counts of the pathogen Prevotella intermedia and did not interfere with the levels of some beneficial species in relation to the negative control. The treatment of multispecies subgingival biofilm with CPC + Zn was effective in controlling periodontal pathogens and favored the colonization of health-associated bacterial species.
Rafael R. Moraes, Carlos E. Cuevas-Suárez, Wilfredo G. Escalante-Otárola, María R. Fernández, Andrés Dávila-Sánchez, Patricia Grau-Grullon, Eduardo Fernández, Tania M. López, Guillermo Grazioli, Luis A. Arana,et al.
Springer Science and Business Media LLC
Abstract Background The COVID-19 pandemic has significantly influenced the routine of healthcare workers. This study investigated the impact of the pandemic on dental practice and dentists’ feelings in Latin America. Methods: A survey was conducted with dentists from 11 Spanish-speaking Latin American countries in September–December 2020. Professionals were invited by email and via an open campaign promoted on social media. The questions investigated dental care routines, practice changes, and feelings about the pandemic. Descriptive statistics were used to identify frequencies and distributions of variables. Proportions were compared using chi-square tests. Results A total of 2127 responses were collected from a sample with diverse demographic, sex, work, and education characteristics. The impact of COVID-19 was considered high/very high by 60% of respondents. The volume of patients assisted weekly was lower compared with the pre-pandemic period (mean reduction = 14 ± 15 patients). A high rate of fear to contracting the COVID-19 at work was observed (85%); 4.9% of participants had a positive COVID-19 test. The main professional challenges faced by respondents were reduction in the number of patients or financial gain (35%), fear of contracting COVID-19 (34%), and burden with or difficulty in purchasing new personal protective equipment (22%). The fear to contracting COVID-19 was influenced by the number of weekly appointments. A positive test by the dentists was associated with their reports of having assisted COVID-19 patients. The most cited feelings about the pandemic were uncertainty, fear, worry, anxiety, and stress. Negative feelings were more prevalent for professionals who did not receive training for COVID-19 preventive measures and those reporting higher levels of fear to contract the disease. Conclusion This multi-country survey indicated a high impact of the pandemic on dental care routines in Latin America. A massive prevalence of bad feelings was associated with the pandemic.
Camila Pinheiro Furquim, Rose Yakushijin Kumagai, Willy Bustillos-Torrez, Jonathan Meza-Mauricio, Caio Junji Tanaka, Veronica Santana, Belen Retamal-Valdes, and Jamil Awad Shibli
Mary Ann Liebert Inc
Tooth loss leads to several oral problems and although a large number of treatments have been proposed to rehabilitate partially or totally edentulous patients, none of them are based on replacement of a missing tooth by a new natural whole-tooth. In the field of tissue engineering, some animal models have been developed to regenerate a natural tooth in the oral cavity. This review shows the state-of-art in whole tooth regeneration based on data from in vivo studies. A systematic scoping review was conducted to evaluate studies that described whole-tooth regeneration and eruption in the oral cavity. The data demonstrated that over 100 animals were used in experimental studies and all of them received implants of tooth germs constructed by bioengineering processes. Mini pigs and pigs were used in 4 studies followed by mice (n=1) and dog (n=1). Over 58 (44%) animals showed whole tooth eruption around 3.5 months after tooth germ implantation (1 to 13.5 months). Most of specimens revealed the presence of odontoblasts, dentin, dentinal tubules, dental pulp, root analogue, cementum, blood vessels and alveolar bone. It could be concluded that in vivo whole tooth regeneration was proved to be possible, but the challenge to overcome translational barriers and test these approaches in humans still remains.
Rafaela D. Parolina de Carvalho, Jéssica de Andrade Moreno, Sindy Magri Roque, Daniel Cheuk Hong Chan, Willy Bustillos Torrez, Rafael Nóbrega Stipp, Bruno Bueno‐Silva, Patricia Oliveira de Lima, and Karina Cogo‐Müller
Wiley
AbstractObjectivesThis study evaluated antimicrobial activity of atorvastatin, pravastatin, rosuvastatin, and simvastatin against oral bacteria, and the interaction of simvastatin with standard antimicrobials (amoxicillin and metronidazole).MethodsMinimal inhibitory concentration assays were performed with Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinomyces odontolyticus, Streptococcus oralis, Streptococcus mitis, Streptococcus salivarius, Streptococcus sanguinis, and Streptococcus gordonii; checkerboard microdilution assays between simvastatin and standard antimicrobials; monospecies and multispecies biofilms.ResultsSimvastatin showed the best antimicrobial activity against most species (MIC range from 3.12 to 25 μg/ml), highlighting the sensitivity of P. gingivalis. In the checkerboard assay, synergistic interaction was found between simvastatin and amoxicillin against S. oralis and S. sanguinis. P. gingivalis biofilm was inhibited by simvastatin at 10 and 50× Minimal inhibitory concentration, with similar effects to metronidazole. For multispecies biofilm, SMV reduced the biofilm metabolic activity (79%) and total counts (87%), comparable to amoxicillin. Simvastatin also reduced bacterial counts of Veilonnella parvula, P. gingivalis, Streptococcus mutans, Actinomyces naeslundii, P. intermedia, and Capnocytophaga ochracea in the multispecies biofilm.ConclusionsSimvastatin showed antimicrobial and antibiofilm activity against oral bacteria and may contribute to the control of dysbiosis, and may be considered in clinical studies as an adjuvant in the treatment of periodontitis.
Jonathan Meza Mauricio, Camila Pinheiro Furquim, Willy Bustillos-Torrez, David Soto-Peñaloza, David Peñarrocha-Oltra, Belen Retamal-Valdes, and Marcelo Faveri
Springer Science and Business Media LLC
Maick Meneguzzo Prado, Davi J. Kovalski, Willy Bustillos Torrez, Bruno Bueno-Silva, Magda Feres, Josiane de Almeida, and Luismar M. Porto
Informa UK Limited
Abstract The objective of this work was to develop a subgingival biofilm model using a stirred bioreactor. Discs of bovine teeth were adapted to a stirred bioreactor filled with a culture medium containing bacterial species associated with periodontal health or disease. After anaerobic incubation, the biofilms growing on the substratum surfaces were collected and analyzed. The mean number of Colony-forming Units (CFUs) varied, but with no difference between 3 and 7 days of biofilm formation (p > 0.05). Scanning Electron Microscopy (SEM) analysis showed a uniform biofilm layer covering the cement layer of the root surface containing bacteria with diverse morphology. In checkerboard DNA-DNA hybridization, bacterial species were identified in both biofilms. In conclusion, a subgingival biofilm model was developed using a stirred bioreactor, allowing the in vitro reproduction of complex microbial communities. This is an advanced model that may be useful to mimic complex clinical periodontal biofilms.