Yulia Sari
Verified @staff.uns.ac.id
Faculty Of Medicine
Universitas sebelas Maret
Yulia Sari is a lecturer at Faculty of Medicine Universitas Sebelas Maret. Her are of expertise is Parasitology and Mycology, Biotechnology, and Molecular Biology. Dr. Yulia Sari received a bachelor degree in Biology in 2003 from Gadjah Mada University, and Postgraduate degree in Biotechnology (M.Si) from Gadjah Mada University in 2006. In 2021, Dr Yulia Sari received her doctoral degree (Dr) from Universitas Sebelas Maret. The research focus is on Parasitology, Mycology, Gut Microbiome, SARS-CoV 2 genomic analysis, Immunologic response caused by SARSCoV2 infection, and Molecular Biology.
EDUCATION
Yulia Sari is a lecturer at Faculty of Medicine Universitas Sebelas Maret. Her are of expertise is Parasitology and Mycology, Biotechnology, and Molecular Biology. Dr. Yulia Sari received a bachelor degree in Biology in 2003 from Gadjah Mada University, and Postgraduate degree in Biotechnology (M.Si) from Gadjah Mada University in 2006. In 2021, Dr Yulia Sari received her doctoral degree (Dr) from Universitas Sebelas Maret.
RESEARCH INTERESTS
Parasitology, Micology, Molecular Biology, Herbal, Medical Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Desy Puspa Putri, Nurhasan Agung Prabowo, and Yulia Sari
AIP Publishing
Nurhasan Agung Prabowo, Yulia Sari, Desy Puspa Putri, and Hartono
AIP Publishing
Paramasari Dirgahayu, Muhana F. Ilyas, Annisa A. Rahma, Shafira N. Hanifa, Matthew A. Wijayanto, Winastari Y. Triniputri, Graciella A. Lukas, Nanang Wiyono, Yulia Sari, and Yusuf A. Mashuri
Narra Sains Indonesia
Human sparganosis, a parasitic infection prevalent in Asia, can progress to cerebral sparganosis, a severe condition with significant neurological symptoms. Diagnosis and treatment are challenging due to its clinical similarity to other infections, highlighting the need for improved detection and management strategies. The aim of this study was to observe research trends, key contributors, gaps in the existing knowledge, diagnosis challenges, effective treatment options, and prevention strategies, providing recommendations for future research directions and clinical practice improvements on cerebral sparganosis. A bibliometric analysis was conducted by extracting 139 documents from the Scopus database in June 2024. The retrieved data were analyzed using the R package's Bibliometrix (Biblioshiny) and VOSviewer. Spanning 97 different sources, the research exhibited an annual growth rate of 2.5%. Annual scientific production revealed fluctuating research activity with peaks in 2010 and 2011 and notable citation peaks in 1996 and 2005, indicating pivotal studies that significantly influenced subsequent research. Early studies focused on diagnosis and specific parasites, while recent studies (2010–2024) have increasingly addressed clinical outcomes, treatment strategies, and advanced diagnostic techniques. Trends revealed a shift towards clinical and diagnostic advancements, with recent emphasis on diagnostic imaging, immunoassays, and the relationship between cerebral sparganosis and brain tumors. In conclusion, the studies on cerebral sparganosis underscore the potential for enhancing clinical practice by improving diagnostic accuracy, informing treatment decisions, and implementing targeted screening efforts based on epidemiology and risk factors. Recommendation to further study needs to notify the cerebral sparganosis in high-risk countries with similar socioeconomic and cultural characteristics to endemic regions, including Indonesia.
Yulia Retno Sari and Maha Rani
AIP Publishing
Devi Ratna Mayasari, Yulia Sari, and Ratih Puspita Febrinasari
Universitas Airlangga
Anemia often occurs in female adolescents cause by iron deficiency. Adolescents are diagnosed anemia if the hemoglobin level is <12 g/dl. Someone who has anemia is prone to have low physical fitness. Consumption of foods that are high in iron and vitamin C such as guava juice can help improve physical fitness. The aim of this study was to analyze the effect of red guava juice and crystal guava juice consumption on physical fitness in female adolescents with anemia. The design of this study was a quasi-experimental with pre and post-test control group design with 36 subjects divided into 3 groups, namely control (iron tablets), P1 (iron tablets + red guava juice), P2 (iron tablets + crystal guava juice). Red guava juice and crystal guava juice given 200 ml per day for 30 days. The physical fitness test uses the Harvard Step Test method with a Physical Efficiency Index assessment. Physical fitness before and after control group had a difference of 1.84±15.86, the difference in P1 group was 17.20±20.35, and the difference in P2 group was of 19.69±15.58. Based on the results of statistical tests, it was found that there were differences in the three groups before and after the intervention p=0.031 (p<0.05). Giving red guava juice and crystal guava juice can be an alternative to improve physical fitness in female adolescents with anemia, but crystal guava juice is the most effective in increasing physical fitness in female adolescents with anemia.
K.S.P. Negara, Y. Sari, S. Haryati, L. Wijayanti, A.H. Anjani, A.N. Faizah, A.P. Jatmiko, and I. MacPhillamy
CRC Press
Ratih D. Yudhani, Yulia Sari, Dwi A. A. Nugrahaningsih, Eti N. Sholikhah, Maftuchah Rochmanti, Abdul K. R. Purba, Husnul Khotimah, Dian Nugrahenny, and Mustofa Mustofa
Hindawi Limited
Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin.
Yulia Sari, Betty Suryawati, Ari Natalia Probandari, Hartono, Wayan Tunas Artama, Bambang Purwanto, and Soetrisno
DiscoverSys, Inc.
Background: Transmission of pathogenic and non-pathogenic microorganisms from animals to humans can affect the composition of gut bacteria (gut microbiome) in humans and can also be a potential source of transmission of zoonotic diseases. Therefore, this study aimed to analyze the gut microbiome interactions between the human and the livestock. Method: This study was a cross-sectional study conducted in Mlati District, Slemanm Yogyakarta. The fecal sample was used to identify parasites by using trichrome staining. Then NGS selected samples were used to analyze the gut microbiome using Illumina high throughput 16S rRNA region V3-V4 sequencing. Result: The analysis showed that Bacteroidetes was prevalent in the HCA group (57%), while Firmicutes (70%) were commonly found in HNC. Regarding the potential parasitic infection, direct microscopic examination and trichrome staining showed that Blastocystis sp and Entamoeba coli cyst was found in several subjects in HCA groups, with prevalence rate at 7.69% and 2.5%. Meanwhile, no parasite was found in HNC feces. Conclusion: There was a significant difference in the gut microbiome and parasitic infections between the HCA and HNC groups, with Blastocystis sp and Entamoeba coli as the most prevalent parasites.
Khesara Sastrin Prasita Negara, Yulia Sari, Lilik Wijayanti, Sri Haryati, Azzahra Harmalia Anjani, An’nurihza Zidhan Azhara, and Azzahra Fadhlila Aulia Nisa
DiscoverSys, Inc.
Infections caused by parasites infect millions of people worldwide, especially in developing countries. Most parasites have a life cycle that lives in hosts (animals or humans) and the environment; thus, the One Health approach is an advanced strategy to overcome these challenges. Recently, One Health has become important as it considers several factors of the connection between humans, animals and the environment. This study aims to understand the One Health Approach as a strategy to prevent and control parasitic zoonosis globally and in Indonesia. We used a literature review using PubMed and Google Scholar based on PRISMA guidelines. Our review identified 22 recommendations and applications of the One Health strategy to handle zoonotic parasitic diseases globally and in Indonesia. There are theoretical and practical gaps in the implementation of this strategy. Further studies are needed to implement better One Health of combating parasitic zoonosis globally and in Indonesia.
Reviono, Hendrastutik, Yulia Sari, Betty Suryawati, Darmawan ismail, and Ketut Putu Yasa
DiscoverSys, Inc.
Background: Coronavirus Disease 2019 (COVID-19) is an easily contagious disease, and not much is known about the characteristics of COVID-19, both in terms of susceptibility, severity, and spreadability of various SARS-CoV-2 strains. Patient genomic factors, especially related to genomic polymorphisms that affect the body's immune system, can influence the course of infectious diseases. The aim of this study is to get an adequate picture regarding gene polymorphisms, both susceptibility and related to the clinical degree in COVID-19 patients. Methods: The PCR preparations were carried out in the Biomedical laboratory of the Faculty of Medicine, Universitas Sebelas Maret. The qualitative PCR (qPCR) examination was sent to the Genetica Science laboratory, Tangerang, West Java. The research subjects were divided into 3 groups, namely COVID-19 patients with no symptoms, COVID-19 patients with mild-moderate symptoms, COVID-19 patients with severe-critical symptoms. The research subjects were taken 6 cc of venous blood (3 cc for examination of serum IL-6 and TNFα levels and 3 cc for DNA examination). Results: Serum levels of IL-6 and TNF-α in the clinical grade group were almost all above normal values. The frequency of TNF- α polymorphisms (-376G/A) all showed homozygote GG. TNF-α (-308G/A) also showed homozygote GG was dominant for SARS CoV2. IL-6 (-572G/C) polymorphism for cases requiring medium and severe clinical degree hospitalization was found to have more C allele than G allele. IL-6 polymorphism (intron A/G) the G allele is less common in cases requiring hospitalization. Conclusion: TNF-α(−308A) allele has an influence on the development of clinical symptoms of SARS CoV2 infection. The rs1800796GG genotype in the IL-6 promoter contributes to milder symptoms in SARS CoV2 infection. Allelic variants of the gene under study may show different effects in other races depending on their interactions with other risk factors.
Hartono Hartono, B Suryawati, Y Sari, A Avicena, Maryani Maryani, C Sukmagautama, H Apriningsih, L Shofiyah, RGH Novika, NJ Wahidah,et al.
EManuscript Technologies
Introduction: To date, no specific therapeutic drug has been approved to target SARS-CoV-2. Hence, it remains a major challenge to decide what potential therapeutic regimens to treat COVID-19 patients. This study aims to investigate curcumin and virgin coconut oil (VCO) effects on cytokine levels (IL-1β, IL-2, IL-6, IL-18, TNF-α, and IFN-β) in COVID-19 patients. Methods: This study was a single-center, controlled trial with a parallel Arm or a Randomized Clinical trial design. A total of sixty COVID-19 patients admitted to the Universitas Sebelas Maret Hospital, Surakarta, Indonesia, were divided into two groups. The first group, consisting of 30 patients, was treated with Azithromycin 500 mg + Oseltamivir 2×75 mg + Hydroxychloroquine 400 mg/day for 5 days. The second group, comprising 30 patients, was treated with Azithromycin 500 mg + Oseltamivir 2×75 mg + Hydroxychloroquine 400 mg/day for 5 days, added with VCO 30 mL and curcumin 3×1 g/day for 21 days. The cytokine profiles of the serum samples were analyzed by the enzyme-linked immunosorbent assay (ELISA) on days 1, 14, and 21. Results: Our study showed that the second group had a significant reduction in IL-1β, IL-2, IL-6, TNF-α, and IFN-β levels after being treated with standard therapy added with curcumin and VCO on day 21 (p<0.05). Conclusion: These results su ested that curcumin and VCO mi ht benefit the treatment of COVID-19 atients
Lilik Wijayanti, Paramasari Dirgahayu, Yulia Sari, Danus Hermawan, and Ida Nurwati
DiscoverSys, Inc.
Open a cess: www.balimedicaljournal.org and ojs.unud.ac.id/index.php/bmj
Yulia Sari, Betty Suryawati, Ari Natalia Probandari, Hartono Hartono, Ratih Dewi Yudhani, and Wayan Tunas Artama
DiscoverSys, Inc.
Abstract: Giardiasis is a gastrointestinal disease caused by the protozoan Giardia sp. Recent data showed that constant contact with livestock could pose greater risk of contracting this disease, especially without any protective gears. Unfortunately, this condition is still prevalent in several areas in Indonesia. Therefore, this study was aimed to evaluate the prevalence of giardiasis and its risk factors in the community around livestock areas and rivers in Mlati, Sleman, DIY, Indonesia. Methods: A cross-sectional study was conducted from JanuaryAugust 2019 around the livestock area and rivers in Mlati, Sleman, DIY. Stool samples were obtained from subjects who have livestock and live along the rivers or its tributaries. The status of Giardiasis were determined from microscopic examination and PCR using beta-giardin as the reference conducted in Parasitology and Biomedical Laboratory, Faculty of Medicine, UNS. Results: A total of 179 subjects were were enrolled in this study. The prevalence of giardiasis among study population was found to be at 5.02% (n=9), 19 (10.6%) of them were cattleman, and 122 (68.1%) had a history of contact with cattle. Subjects who did not own any cattle seemed to have a significantly lower risk of contracting giardiasis (AOR: 0.041, 95%CI: 0.009-0.185; p=0.000) since contact with cattle also proved to be the risk factor of giardiasis (AOR: 1.080, 95%CI: 1.027-1.135; p<0.002). Conclusion: This study revealed that constant contact with cattle lead to significantly higher risk of giardiasis for those who live among livestock and along the rivers in Mlati, Sleman DIY. Giardiasis is a gastrointestinal disease caused by the protozoan Giardia sp. Recent data showed that constant contact with livestock could pose greater risk of contracting this disease, especially without any protective gears. Unfortunately, this condition is still prevalent in several areas in Indonesia. Therefore, this study was aimed to evaluate the prevalence of giardiasis and its risk factors in the community around livestock areas and rivers in Mlati, Sleman, DIY, Indonesia. Methods: A cross-sectional study was conducted from JanuaryAugust 2019 around the livestock area and rivers in Mlati, Sleman, DIY. Stool samples were obtained from subjects who have livestock and live along the rivers or its tributaries. The status of Giardiasis were determined from microscopic examination and PCR using beta-giardin as the reference conducted in Parasitology and Biomedical Laboratory, Faculty of Medicine, UNS. Results: A total of 179 subjects were were enrolled in this study. The prevalence of giardiasis among study population was found to be at 5.02% (n=9), 19 (10.6%) of them were cattleman, and 122 (68.1%) had a history of contact with cattle. Subjects who did not own any cattle seemed to have a significantly lower risk of contracting giardiasis (AOR: 0.041, 95%CI: 0.009-0.185; p=0.000) since contact with cattle also proved to be the risk factor of giardiasis (AOR: 1.080, 95%CI: 1.027-1.135; p<0.002). Conclusion: This study revealed that constant contact with cattle lead to significantly higher risk of giardiasis for those who live among livestock and along the rivers in Mlati, Sleman DIY.
Afiono Agung Prasetyo and Yulia Sari
IOP Publishing
Sri Haryati, Afiono Agung Prasetyo, Yulia Sari, and Ruben Dharmawan
IOP Publishing
Toxoplasma gondii Surface Antigen 1 (SAG1) is often used as a diagnostic tool due to its immunodominant-specific as antigen. However, data of the Toxoplasma gondii SAG1 protein from Indonesian isolate is limited. To study the protein, genomic DNA was isolated from a Javanese acute toxoplasmosis blood samples patient. A complete coding sequence of Toxoplasma gondii SAG1 was cloned and inserted into an Escherichia coli expression plasmid and sequenced. The sequencing results were subjected to bioinformatics analysis. The Toxoplasma gondii SAG1 complete coding sequences were successfully cloned. Physicochemical analysis revealed the 336 aa of SAG1 had 34.7 kDa of weight. The isoelectric point and aliphatic index were 8.4 and 78.4, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. The results of this study would contribute information about Toxoplasma gondii SAG1 and benefits for further works willing to develop diagnostic and therapeutic strategies against the parasite.
Afiono Agung Prasetyo, Yulia Sari, Ruben Dharmawan, and Marwoto
Author(s)
Sexual contact and other risk behavior among transgender working as commercial sex workers are important factors for sexual and blood-borne virus (BBV) infections. However, there no data concerning the molecular status of human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) circulated among transgender working as commercial sex workers. Blood samples obtained from transgender working as commercial sex workers in Surakarta were examined for HIV antibodies, HBsAg and HCV antibodies, respectively, by immunological assays. All blood samples were also subjected for viral nucleic acid extraction and molecular detection of HIV, HBV and HCV by nested RT-PCR. The PCR products were purified from agarose gels, and the nucleotide sequences were retrieved and molecular analyzed. HIV, HBV and HCV was detected in 26.9% (7/26), 19.2% (5/26) and 46.2% (12/26), respectively. HIV CRF01_AE and B were found to be circulating in the community. HBV genotype B3 predominated, followed by C1. HCV...
Afiono Agung Prasetyo, Ruben Dharmawan, Yulia Sari, and Ratna Sariyatun
Author(s)
Human immunodeficiency virus type 1 (HIV-1) remains a cause of global health problem. Continuous studies of HIV-1 genetic and immunological profiles are important to find strategies against the virus. This study aimed to conduct analysis of sequence conservation, HLA-E-restricted peptide, and best-defined CTL/CD8+ epitopes in p24 (capsid) of HIV-1 subtype B worldwide. The p24-coding sequences from 3,557 HIV subtype B isolates were aligned using MUSCLE and analysed. Some highly conserved regions (sequence conservation ≥95%) were observed. Two considerably long series of sequences with conservation of 100% was observed at base 349-356 and 550-557 of p24 (HXB2 numbering). The consensus from all aligned isolates was precisely the same as consensus B in the Los Alamos HIV Database. The HLA-E-restricted peptide in amino acid (aa) 14-22 of HIV-1 p24 (AISPRTLNA) was found in 55.9% (1,987/3,557) of HIV-1 subtype B worldwide. Forty-four best-defined CTL/CD8+ epitopes were observed, in which VKNWMTETL epitope (aa 18...
Afiono Agung Prasetyo, Sabrina Damara Luvi, Hartono, and Yulia Sari
Author(s)
The molecular epidemiology data of torque teno virus (TTV) in Indonesia is very rare. This study evaluated the prevalence of TTV in male commercial sex workers, as one of the high risk community for blood borne viruses pathogens in Surakarta, Indonesia. All blood samples collected from male commercial sex workers in Surakarta in 2009-2013 were tested by nested polymerase chain reaction (PCR). The amplified PCR products were molecularly cloned and subjected to sequence analysis. TTV DNA was detected in 80.9% (72/89) samples. Furthermore, the molecular characterization revealed that the most prevalent was genogroup 3, followed by genogroup 2 and l, respectively. TTV was detected in male commercial sex workers in Surakarta with high infection rate. Further investigation about TTV circulation in Indonesian population is needed in order to provide additional information about the genetic variability and TTV epidemiology in Indonesia, especially in the high risk communities.
Afiono Agung Prasetyo and Yulia Sari
Author(s)
Human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are retroviruses that probably among the most neglected blood-borne pathogens. The molecular epidemiology data of HTLV-1/2 in Indonesia is very rare. This study evaluated the prevalence of HTLV-1 and 2 in men who have sex with men with drug abused history in Surakarta Indonesia, to track the presentation of HTLV-1/2 in Indonesia. All blood samples collected from men who have sex with men with drug abused history in Surakarta in 2009-2013 were tested using enzyme linked immunosorbent assays and confirmed by RT-PCR nested addressed the part of HTLV-1 LTR and HTLV-2 LTR region, respectively. The specificity of the molecular assays was confirmed by sequencing the amplicons. The anti HTLV-1/2 positive rate was 4.8% (6/126). All positive serological samples were confirmed by nested RT-PCR. Of these, two was HTLV-1 positive and four was HTLV-2 positive. Molecular analysis of positive PCR products revealed that all HTLV-1 isolate had close relationship with HTLV-1 isolated in Japan while all HTLV-2 isolate with that of isolated in USA. HTLV-1 and HTLV-2 were detected in men who have sex with men with drug abused history in Surakarta indicated that these viruses were circulated in Indonesia, especially in the high risk communitiesHuman T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are retroviruses that probably among the most neglected blood-borne pathogens. The molecular epidemiology data of HTLV-1/2 in Indonesia is very rare. This study evaluated the prevalence of HTLV-1 and 2 in men who have sex with men with drug abused history in Surakarta Indonesia, to track the presentation of HTLV-1/2 in Indonesia. All blood samples collected from men who have sex with men with drug abused history in Surakarta in 2009-2013 were tested using enzyme linked immunosorbent assays and confirmed by RT-PCR nested addressed the part of HTLV-1 LTR and HTLV-2 LTR region, respectively. The specificity of the molecular assays was confirmed by sequencing the amplicons. The anti HTLV-1/2 positive rate was 4.8% (6/126). All positive serological samples were confirmed by nested RT-PCR. Of these, two was HTLV-1 positive and four was HTLV-2 positive. Molecular analysis of positive PCR products revealed that all HTLV-1 isolate had close relationship with...
Yulia Sari, Sri Haryati, Afiono Agung Prasetyo, Hartono, and Zainal Arifin Adnan
Author(s)
The human leukocyte antigen (HLA)-DQB1 gene polymorphisms may associated with the infection risk of Toxoplasma gondii in HIV patients. The HLA-DQB1*0402 in HIV-1-positive patients could be considered risk factors for developing neurological opportunistic infections, mainly Toxoplasma encephalitis. However, the HLA-DQB1*0402 gene polymorphisms status in the Javanese HIV patients is unknown. This study evaluated the prevalence of HLA-DQB*0402 alleles polymorphisms in Javanese HIV patients with positive anti-Toxoplasma gondii IgM status. Since 2009 our research group performing a molecular epidemiology of blood borne viruses in Central Java Indonesia, by collecting the epidemiological and clinical data from the high risk communities. All blood samples were screened for blood borne pathogens by serological and molecular assays including for HIV and Toxoplasma gondii. The genomic DNA was isolated from the whole blood samples. Genetic polymorphisms of HLA-DQB1*0402 alleles were detected with polymerase chain re...
S Haryati, Y Sari, A A Prasetyo, and R Sariyatun
IOP Publishing
Diagnosis and identification of the genetic group of T. gondii (Toxoplasma gondii) are important to control better the T. gondii infection, particularly in immunocompromised people as HIV patients. This study aimed to identify strain-specific variations in T. gondii GRA1, GRA5, GRA6, GRA8, and GRA14 in order to help design such diagnostic tool to detect and characterize the parasite. Forty-three T. gondii GRA1, GRA5, GRA6, GRA8, and GRA14 sequences deposited in GenBank were aligned. A number of positions in the gene sequences were highly conserved. All GRA sequences had strain-specific positions, however, only GRA1, GRA5, and GRA6, which contained specific variations for each T. gondii lineage. In conclusion, T. gondii GRA1, GRA5, GRA6, GRA8, and GRA14 are predicted to contain highly conserved regions and positions with strain-specific variation, which might be useful for the design of diagnostic tools detecting and distinguishing T. gondiistrains.
S Haryati, Y Sari, A APrasetyo, and R Sariyatun
IOP Publishing
The dense granular (GRA) proteins of Toxoplasma gondii(T. gondii) have been demonstrated as potential sources of T. gondii vaccine antigens. However, data of the GRA5 protein are limited. This study analyzed twenty-one complete GRA5 sequences of T. gondii GT1, RH, ME49, VEG, MAS, RUB, FOU, p89, VAND, and GAB2-2007-GAL-DOM2 strains to identify potential epitopes restricted by Major Histocompatibility Complex class II (MHC- II) molecules (human leukocyte antigen (HLA)-DP, HLA-DQ, and HLA-DR) in the protein. In all T. gondii strains, peptides positioned at amino acid (aa) 15-29, 16-30, 17-31, 18-32, 19-33, 83-97, 84-98, 86-100, 87-101, 89-103, and 90-104 were predicted to pose high affinity and binding with HLA-DRB1*0101, HLA-DRB1*0301 (DR17), HLA-DRB1*0401 (DR4Dw4), HLA-DRB1*0701, HLA-DRB1*1101, HLA-DRB1*1501 (DR2b), and/or HLA-DRB5*0101. Considering the epitope's affinity, ligation strength, and hydrophilicity, LRLLRRRRRRAIQEE sequence (aa 90-104) restricted by HLA-DRB1*0101, HlA- DRB1*0301 (DR17), and HLA-DRB1*0401 (DR4Dw4) was considered as the most potential MHC-II epitope in GRA5 of T. gondii. These results would be useful for studies concerning in developing T. gondii vaccine and diagnostic method.