Katerina Komrskova

@ibt.cas.cz

Reproductive Biology
Institute of Biotechnology of the Czech Academy of Sciences, Czech Republic

Katerina Komrskova


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https://researchid.co/a-8705-2010

RESEARCH INTERESTS

Selecting markers associated with human infertility and designing diagnostic kits for clinical practice, sperm-egg interaction and fusion; sperm preparation for fertilization (capacitation and acrosome reaction); sperm-egg interaction; epigenetic regulation of early embryogenesis, epigenetic markers associated with human infertility; spermatogenesis and sperm parameters assessment after pathological challenge including cancer, genomic and proteomic approach.
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Scopus Publications

Scopus Publications

  • Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality
    Tereza Otčenášková, Eliška Macíčková, Jana Vondráková, Michaela Frolíková, Katerina Komrskova, et al.
    European Journal of Cell Biology, 2023
  • Timing of ICSI with Respect to Meiotic Spindle Status
    Olga Tepla, Zinovij Topurko, Simona Jirsova, Martina Moosova, Eva Fajmonova, et al.
    International Journal of Molecular Sciences, 2023
    The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5–6 h after ovum pick-up (OPU) or 7–8 h after OPU. Oocytes without MS evaluation were all fertilized 5–6 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58/182); while 195 patients without MS visualization had 24% PR (47/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58/140), while 162 patients without MS visualization had 41% PR (66/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
  • Sperm morphology and performance in relation to postmating prezygotic isolation in two recently diverged passerine species
    Manon Poignet, Lucie Baránková, Jiří Reif, Pavel Stopka, Romana Stopková, et al.
    Scientific Reports, 2022
    Divergence in sperm phenotype and female reproductive environment may be a common source of postmating prezygotic (PMPZ) isolation between species. However, compared to other reproductive barriers it has received much less attention. In this study, we examined sperm morphology and velocity in two hybridizing passerine species, the common nightingale (Luscinia megarhynchos) and thrush nightingale (L. luscinia). In addition, we for the first time characterized a passerine female reproductive tract fluid proteome. We demonstrate that spermatozoa of the common nightingale have significantly longer and wider midpiece (proximal part of the flagellum containing mitochondria) and longer tail compared to spermatozoa of thrush nightingale. On the other hand, they have significantly shorter and narrower acrosome. Importantly, these differences did not have any effect on sperm velocity. Furthermore, the fluid from the reproductive tract of common nightingale females did not differentially affect velocity of conspecific and heterospecific sperm. Our results indicate that the observed changes in the flagellum and acrosome size are unlikely to contribute to PMPZ isolation through differential sperm velocity of conspecific and heterospecific sperm in the female reproductive tract. However, they could affect other postcopulatory processes, which might be involved in PMPZ isolation, such as sperm storage, longevity or sperm-egg interaction.
  • Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
    Nour El Houda Mimouni, Côme Ialy-Radio, Anne-Lyse Denizot, Isabelle Lagoutte, Michaela Frolikova, et al.
    International Journal of Molecular Sciences, 2022
    Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm–oocyte adhesion/fusion and early implantation in the absence of sperm β1 integrin. Males and females with β1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm β1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin β1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern.
  • Dynamic study of small toxic hydrophobic proteins PepA1 and PepG1 of Staphylococcus aureus
    Vishma Pratap Sur, Ondrej Simonik, Michaela Novotna, Aninda Mazumdar, Frantisek Liska, et al.
    International Journal of Biological Macromolecules, 2022
  • H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin
    Alberto de la Iglesia, Paula Jauregi, Meritxell Jodar, Ferran Barrachina, Lukas Ded, et al.
    International Journal of Molecular Sciences, 2022
    Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote.
  • MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization
    Jana Vondrakova, Michaela Frolikova, Lukas Ded, Jiri Cerny, Pavla Postlerova, et al.
    Science Advances, 2022
    Gamete fusion is a critical event of mammalian fertilization. A random one-bead one-compound combinatorial peptide library represented synthetic human egg mimics and identified a previously unidentified ligand as Fc receptor–like 3, named MAIA after the mythological goddess intertwined with JUNO. This immunoglobulin super family receptor was expressed on humanoolemmaand played a major role during sperm-egg adhesion and fusion. MAIA forms a highly stable interaction with the known IZUMO1/JUNO sperm-egg complex, permitting specific gamete fusion. The complexity of the MAIA isotype may offer a cryptic sexual selection mechanism to avoid genetic incompatibility and achieve favorable fitness outcomes.
  • Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation
    Tereza Bosakova, Antonin Tockstein, Zuzana Bosakova, Katerina Komrskova
    Molecules, 2022
    17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added αE2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations Bt were subjected to kinetic analysis. The findings indicated that αE2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.
  • Boar Sperm Cryopreservation Improvement Using Semen Extender Modification by Dextran and Pentaisomaltose
    Ondrej Simonik, Filipa Bubenickova, Lucie Tumova, Michaela Frolikova, Vishma Pratap Sur, et al.
    Animals, 2022
    The long-term storage of boar sperm presents an ongoing challenge, and the modification of the cryoprotective compounds in semen extenders is crucial for improving cryopreservation’s success rate. The aim of our study was to reduce the percentage of glycerol in the extender by elimination or substitution with biocompatible, non-toxic polysaccharides. For boar semen extender improvement, we tested a novel modification with the polysaccharides dextran and pentaisomaltose in combination with unique in silico predictive modeling. We targeted the analysis of in vitro qualitative sperm parameters such as motility, viability, mitochondrial activity, acrosome integrity, and DNA integrity. Non-penetrating polysaccharide-based cryoprotective agents interact with sperm surface proteins such as spermadhesins, which are recognized as fertility markers of boar sperm quality. The in silico docking study showed a moderate binding affinity of dextran and pentaisomaltose toward one specific spermadhesin known as AWN, which is located in the sperm plasma membrane. Pentaisomaltose formed a hydrophobic pocket for the AWN protein, and the higher energy of this protein–ligand complex compared with dextran was calculated. In addition, the root mean square deviation (RMSD) analysis for the molecular dynamics (MD) of both polysaccharides and AWN simulation suggests their interaction was highly stable. The in silico results were supported by in vitro experiments. In the experimental groups where glycerol was partially or entirely substituted, the use of pentaisomaltose resulted in improved sperm mitochondrial activity and DNA integrity after thawing when compared with dextran. In this paper, we demonstrate that pentaisomaltose, previously used for cryopreservation in hematopoietic stem cells, represents a promising compound for the elimination or reduction of glycerol in extenders for boar semen cryopreservation. This novel approach, using in silico computer prediction and in vitro testing, represents a promising technique to help identify new cryoprotectants for use in animal breeding or genetic resource programs.
  • Specific inhibition of VanZ-mediated resistance to lipoglycopeptide antibiotics
    Vishma Pratap Sur, Aninda Mazumdar, Vladimir Vimberg, Tommaso Stefani, Ladislav Androvic, et al.
    International Journal of Molecular Sciences, 2022
    Teicoplanin is a natural lipoglycopeptide antibiotic with a similar activity spectrum as vancomycin; however, it has with the added benefit to the patient of low cytotoxicity. Both teicoplanin and vancomycin antibiotics are actively used in medical practice in the prophylaxis and treatment of severe life-threatening infections caused by gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, Enterococcus faecium and Clostridium difficile. The expression of vancomycin Z (vanZ), encoded either in the vancomycin A (vanA) glycopeptide antibiotic resistance gene cluster or in the genomes of E. faecium, as well as Streptococcus pneumoniae and C. difficile, was shown to specifically compromise the antibiotic efficiency through the inhibition of teicoplanin binding to the bacterial surface. However, the exact mechanisms of this action and protein structure remain unknown. In this study, the three-dimensional structure of VanZ from E. faecium EnGen0191 was predicted by using the I-TASSER web server. Based on the VanZ structure, a benzimidazole based ligand was predicted to bind to the VanZ by molecular docking. Importantly, this new ligand, named G3K, was further confirmed to specifically inhibit VanZ-mediated resistance to teicoplanin in vivo.
  • Role of integrins in sperm activation and fertilization
    Veronika Merc, Michaela Frolikova, Katerina Komrskova
    International Journal of Molecular Sciences, 2021
  • In silico identification and validation of organic triazole based ligands as potential inhibitory drug compounds of sars-cov-2 main protease
    Vishma Pratap Sur, Madhab Kumar Sen, Katerina Komrskova
    Molecules, 2021
  • αV Integrin Expression and Localization in Male Germ Cells
    Veronika Palenikova, Michaela Frolikova, Eliska Valaskova, Pavla Postlerova, Katerina Komrskova
    International Journal of Molecular Sciences, 2021
  • Important parameters affecting quality of vitrified donor oocytes
    Olga Teplá, Zinovij Topurko, Jaromír Mašata, Simona Jirsová, Michaela Frolíková, et al.
    Cryobiology, 2021
  • Kinetic study of 17α-estradiol activity in comparison with 17β-estradiol and 17α-ethynylestradiol
    Tereza Bosakova, Antonin Tockstein, Zuzana Bosakova, Katerina Komrskova
    Catalysts, 2021
  • Erratum: Platelets facilitate the wound-healing capability of mesenchymal stem cells by mitochondrial transfer and metabolic reprogramming (Cell Metabolism (2021) 33(2) (283–299.e9), (S1550413120306616), (10.1016/j.cmet.2020.12.006))
    Jennyfer Levoux, Alexandre Prola, Peggy Lafuste, Marianne Gervais, Nathalie Chevallier, et al.
    Cell Metabolism, 2021
  • Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming
    Jennyfer Levoux, Alexandre Prola, Peggy Lafuste, Marianne Gervais, Nathalie Chevallier, et al.
    Cell Metabolism, 2021
  • Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity
    Hwang I. S. Thomas, Ying-Shiuan Chen, Ching-Han Hung, Dilip Bhargava Sreerangaraja Urs, Tien-Ling Liao, et al.
    Oxidative Medicine and Cellular Longevity, 2021
  • The role of the linc complex in sperm development and function
    Vera Kmonickova, Michaela Frolikova, Klaus Steger, Katerina Komrskova
    International Journal of Molecular Sciences, 2020
  • Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells
    J. Jankovicova, M. Frolikova, V. Palenikova, E. Valaskova, J. Cerny, et al.
    Scientific Reports, 2020
  • Correction to: Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos (Medical Microbiology and Immunology, (2020), 209, 4, (407-425), 10.1007/s00430-020-00676-0)
    Jana Jankovičová, Zdeňka Neuerová, Petra Sečová, Michaela Bartóková, Filipa Bubeníčková, et al.
    Medical Microbiology and Immunology, 2020
  • Gestational and pubertal exposure to low dose of di-(2-ethylhexyl) phthalate impairs sperm quality in adult mice
    Pavla Dostalova, Eva Zatecka, Lukas Ded, Fatima Elzeinova, Eliska Valaskova, et al.
    Reproductive Toxicology, 2020
  • Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos
    Jana Jankovičová, Zdeňka Neuerová, Petra Sečová, Michaela Bartóková, Filipa Bubeníčková, et al.
    Medical Microbiology and Immunology, 2020
  • Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility
    Dilip Bhargava Sreerangaraja Urs, Wen-Han Wu, Katerina Komrskova, Pavla Postlerova, Yung-Feng Lin, et al.
    International Journal of Molecular Sciences, 2020
  • The role of taste receptor mTAS1R3 in chemical communication of gametes
    Michaela Frolikova, Tereza Otcenaskova, Eliska Valasková, Pavla Postlerova, Romana Stopkova, et al.
    International Journal of Molecular Sciences, 2020