A Novel Spectrophotometric Method for Determining the Peroxide Value of Edible Oils Mahmoud Hussein Hadwan, Inas J. Mahdi, Rawaa Hefdhi Zaooli, Asad M. Hadwan, Rawa M. Mohammed, et al. Efood, 2026 This study introduces a novel spectrophotometric method to sensitively and specifically determine the peroxide value of edible oils, specifically engineered to overcome the significant analytical interference of carotenoid pigments that have historically compromised traditional methods. The method uses a solution containing ferrous (Fe 2+ ) ions and either salicylic acid (SA) or sulfosalicylic acid (SSA). Next, these Fe 2+ ions are reacted with a sample of edible oil in the presence of peroxide, forming ferric (Fe³⁺) ions. In the next step, the Fe³⁺ ion forms a complex with SSA (or SA), resulting in a ferrisulfosalicylate (or sulfosalicylate) complex that exhibits a unique color. This color change was utilized for measuring lipid peroxides. It provides a rapid, simple, and sensitive method for detecting and quantifying peroxides. SSA and SA can form complexes that absorb light at 505 and 525 nm, respectively. When this new method was compared to Ferrous Oxidation‐Xylenol Orange (FOX) and ferrithiocyanate methods, it showed an impressive correlation (Pearson's r = 0.99). This study demonstrates that the proposed method is effective in determining the peroxide value in the various types of edible oils examined.
Clinical Parameters and Association of CCHCR1 Single-Nucleotide Polymorphisms in Psoriasis Arthritis Patients Ahmed Al-Mukhtar, Muhsin H. Ubeid, Naseer Al-Mukhtar, Abdulsamie Hassan Alta’ee Medical Journal of Babylon, 2025 Background: Previous information states that autoimmune and chronic inflammatory diseases, such as psoriasis and a psoriasis arthritis (PsA), mostly identified by multiple idiopathic cause. Objective: The current study resulted in new positive and significance changes in serum biochemical indicators for the patient and healthy participant groups. Materials and Methods: The present study is a case-control study represented by two groups (the first group 50 patients, while 40 healthy persons where been and the second group). Clinical biochemical parameters were symbolized by Vitamin K2 (estimated by the ELISA technique) and the reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio was evaluated using high-performance liquid chromatography. Results: The outcomes of the genetic parameter study of (CCHCR1 Gene) revealed a clear polymorphism for the (rs3130453) single nucleotide polymorphism in the first group. Polymerase chain reaction (REFLIP) type and gel electrophoresis were used as techniques for genetic examination. Levels of serum Vitamin K2 and GSH parameters were decreased in the PsA group, where’s as present, in normal level of healthy group. Meantime, the study explained an increase in the level of serum GSSG for PsA group, while, still normal in the second group. Conclusion: The actual study shows that an elevation of oxidative stress, which in final clarify and concludes a low GSH/GSSG ratio with the genetic polymorphism studied group.
Studying the Levels of Copeptin and Cortisol as a Stress Monitoring Biomarkers in the Patients with Alopecia Areata in Babylon Governorate Rasool Mahmoud Hashem, Abdulsamie Hassan Alta’ee, Wessam Ali Awath Medical Journal of Babylon, 2025 Background: Alopecia areata (AA) can manifest clinically as little areas of hair loss or as total loss of scalp and body hair. Stress is a common cause of hair loss. In response, the adrenal gland secretes glucocorticoids and catecholamines, which affect the immune system by preventing extensive activation, the metabolism by providing acute energy, and the vasculature by increasing blood pressure. Objective: The aim of this research is to investigate the potential role of stress in the pathophysiology of AA. Additionally, to elucidate the potential use of a stress monitoring biomarker as a diagnostic indicator for AA. Materials and Methods: An investigation using a case–control method involved 60 male patients with AA and 60 male controls in good condition. In addition, the individuals with AA were separated into two age groups: adults and non-adults. Blood samples were obtained to measure copeptin and cortisol. Age and body mass index were evaluated in addition to other variables. Results: This study found that copeptin and cortisol concentrations in both adult and pediatric patients increased significantly (P < 0.05) compared to the control group. The area under the curve (AUC) for adult copeptin was 0.841 and 0.759 in children, while the AUC for cortisol was 0.878 in adults and 0.872 in children. Conclusions: According to this study, copeptin and cortisol could be key players in the pathophysiology of AA.
Determination of Vitamin K2, Lipoprotein (a) and NTerminal pro Brain Natriuretic Biomarkers in Diabetic Hypertensive Patients with Acute Myocardial Infarction for Clinical Intervention Journal of Angiotherapy, 2024 Background: Severe Myocardial Infarction is a highly stark manifestation of heart illness, often accompanied with additional complications such as diabetes mellitus (DM) and hypertension (HTN). The hormone within the body enables glucose to enter the cells and burn them up as fuel. HTN is a common vascular disorder that represents a major cardiovascular condition. Methods: The study was conducted at the Biochemistry Department of the College of Medicine from April 2022 to May 2023 and used the Human Vit K2 ELISA Kit, Human Lp(a) ELISA Kit, and Human N-terminal ProBST ELISA Kit to estimate the serum levels of vitamin K2, lipoprotein (a) (Lp(a)), and N-terminal of the Pro brain natriuretic peptide (NT-proBNP). Results: The results showed a important decrease in VK2 levels in AMI patients with DM and HTN than the other two groups (less than AMI patients group with and without HDL-c). On the other hand, Lp(a), and NT-pro BNP levels were significantly increased in AMI patients with DM and HTN than the other two groups (less than AMI patients group with no DM and HTN). As the diagnostic accuracy of tests increased, the area under the ROC curve for NTproBNP for diagnosing AMI achieved the highest 0.988. NTproBNP showed a promising ability to differentiate AMI patients from DM+ HTN group with 0.971 (AUC). Conclusions: In diabetic hypertensive patients with acute myocardial infarction, a low level of vitamin K2 and a high level of lipoprotein(a) might be risk markers for the onset of myocardial infarction.
Detection of Receptor for Advanced Glycation End Products (RAGE) and High Mobility Group Protein Box 1 HMGB1 Levels in Patients’ Serum with Breast Cancer Hind Shawqi Zaki, Abdulsamie Hassan Alta’ee, Mushtaq Qahtan Mohammed Medical Journal of Babylon, 2023 Background: Breast cancer (BC) is an unchecked proliferation of epithelial cells that begin in the breast lobules or ducts. BC develops and spreads as a result of the high mobility group protein box 1 (HMGB1). The survival, development, and metastasis of tumor cells have all been analyzed for the patients from Oncology Center in Merjan Medical City, Babylon Governorate. HMGB1 and receptor for advanced glycation end products (RAGE) levels in patients and controls were assessed using the enzyme-linked immunosorbent assay technique. Objectives: The current study’s goal is to analyze the blood levels of HMGB1 and RAGE in both BC patients and healthy volunteers and evaluate how their expression changes as the disease progresses. Materials and Methods: Samples collected from BC levels exhibited a 76% sensitivity and a 70% specificity, respectively. Serum RAGE levels were 74% sensitive and 70% specific for the diagnosis of BC, respectively, and their substantial P value = 0.023 correlated with tumor size. Results: Patients had significantly higher HMGB1 and RAGE levels than did the healthy control group. In order to identify BC, serum HMGB1 is linked to HMGB1 binding to the RAGE receptor. Conclusion: The presence of HMGB1 in the serum may serve as a helpful biomarker for the detection of BC. BC RAGE is useful for monitoring the growth of tumor size.
Association of HLA-DRB1 gene polymorphism in rheumatoid arthritis patients in Babylon Province, Iraq Indian Journal of Forensic Medicine and Toxicology, 2020
Association of oxidative stress and disease activity in rheumatoid arthritis patients in babylon province Medico Legal Update, 2020
ASSESSMENT OF CIRCULATING ESTROGEN AND OSTEOPROTEGERIN LEVELS IN OSTEOPOROSIS POST-MENOPAUSAL WOMEN IN BABYLON PROVINCE Biochemical and Cellular Archives, 2020
EFFECT OF GLUTATHIONE-S-TRANSFERASE ACTIVITY AND GENE POLYMORPHISMS ON PROSTATE CANCER IN BABYLON PROVINCE OF IRAQ Biochemical and Cellular Archives, 2020
Novel CUPRAC-based spectrophotometric method for rapid and eco-friendly determination of cellulase activity IJ Mahdi, ZA Al Talebi, AM Hadwan, AH Alta’ee, AT Al-Hassnawi, ... Cellulose, 1-19 , 2026 2026
A Novel Spectrophotometric Method for Determining the Peroxide Value of Edible Oils MH Hadwan, IJ Mahdi, RH Zaooli, AM Hadwan, RM Mohammed, ... eFood 7 (1), e70116 , 2026 2026
Studying the Levels of Copeptin and Cortisol as a Stress Monitoring Biomarkers in the Patients with Alopecia Areata in Babylon Governorate RM Hashem, AH Alta’ee, WA Awath Medical Journal of Babylon 22 (2), 604-609 , 2025 2025
Clinical Parameters and Association of CCHCR1 Single-Nucleotide Polymorphisms in Psoriasis Arthritis Patients A Al-Mukhtar, MH Ubeid, N Al-Mukhtar, AH Alta’ee Medical Journal of Babylon 22 (Suppl 1), S140-S146 , 2025 2025
Study the Levels of as a Stress Monitoring Adrenaline and Prolactin Biomarkers in the Patients with Alopecia Areata in Babylon Governorate RM Hashem, AH Alta'ee, WA Ameen 2025
Study the Levels of as a Stress Monitoring Adrenaline and Prolactin Biomarkers in the Patients with Alopecia Areata in Babylon Governorate RasoolMahmoudHashem, AH Alta'ee, WA Ameen Journal of Kufa for Chemical Sciences 4 (2), 169-180 , 2025 2025
A new spectrophotometric method for measuring ceruloplasmin ferroxidase activity: an innovative approach MH Hadwan, AK Rahi, ER Abass, AM Hadwan, RM Mohammed, ... BioMetals 37 (6), 1699-1712 , 2024 2024 Citations: 2
Determination of Vitamin K2, Lipoprotein (a) and N-Terminal pro Brain Natriuretic Peptide in Diabetic Hypertension with Acute Myocardial Infarction Patients in Babylon Governorate G Jawad, A Alta'ee, O Alsalihi 5th International Conference on Biomedical and Health Sciences, 500-504 , 2024 2024
An efficient protocol for quantifying catalase activity in biological samples MH Hadwan, AH Alta’ee, RM Mohammed, AM Hadwan, HS Al-Kawaz, ... Bulletin of the National Research Centre 48 (1), 34 , 2024 2024 Citations: 17
Determination of Vitamin K2, Lipoprotein (a) and N-Terminal pro Brain Natriuretic Biomarkers in Diabetic Hypertensive Patients with Acute Myocardial Infarction for Clinical … GK Jawad, AH Alta'ee, OJ Alsalihi Journal of Angiotherapy 8 (2), 1-8 , 2024 2024 Citations: 1
Detection of Receptor for Advanced Glycation End Products (RAGE) and High Mobility Group Protein Box 1 HMGB1 Levels in Patients’ Serum with Breast Cancer MQM Hind Shawqi Zaki, Abdulsamie Hassan Alta’ee Medical Journal of Babylon 20 (4), 847-851 , 2024 2024
Study the Effect of RAGE and HMGB1 Genes Polymorphism on Breast Cancer Susceptibility in Iraqi Female H Zaki, AH Alta'ee, MK Mohammed Journal of Kufa for Chemical Sciences 3 (1), 211-222 , 2023 2023
Novel fluorometric protocol for assessing myeloperoxidase activity R Majid, ZA Al Talebi, HS Al-Kawaz, AH Alta'ee, ARS Alsalman, ... Enzyme and Microbial Technology 171, 110320 , 2023 2023 Citations: 11
Low levels of peroxiredoxins are associated with high iron content and lipid peroxidation in seminal plasma from asthenozoospermic infertile men LA Almashhedy, HA Fadhil, ARS Alsalman, HS Al-Kawaz, AH Alta'ee, ... Endocrine and Metabolic Science 12, 100137 , 2023 2023 Citations: 9
Impact of lipid-lowering drugs on lipid profile in hyperlipidemia patients KA Shemran, SH Edin, AH Alta’ee Medical Journal of Babylon 20 (2), 274-277 , 2023 2023 Citations: 6
DETERMINATION OF VITAMIN D3 FORMS AND VITAMIN D RECEPTORS IN DIABETIC HYPERTENSION WITH ACUTE MYOCARDIAL INFARCTION PATIENTS IN BABYLON GOVERNORATE GK Jawad, AH Alta'ee, OJ Alsalihi Romanian Journal of Diabetes, Nutrition and Metabolic Diseases 30 (4), 1037-1045 , 2023 2023
The Alteration in Parathyroid Hormone and Calcitonin of Pregnant Women in Babylon Governorate HS Hammadi, AH Alta’ee, BA Musa JOURNAL OF BIOMEDICINE AND BIOCHEMISTRY 2 (2), 9–16 , 2023 2023 Citations: 9
Long-Term Use of Glucocorticoid Modulated Parathyroid Hormone Levels in Osteoporosis Patients AJ Kadam, AH Alta'ee, AH Al-Handawy, ZM Al-Ghazali, MJ Ewadh GEORGIAN MEDICAL NEWS 4 (337), 13-15 , 2023 2023
Simple spectrophotometric method for the assessment of aspirin esterase activity AAR Jawad, ZA Al Talebi, AH Alta’ee, AM Hadwan, MA Abdulmahdi, ... Monatshefte für Chemie-Chemical Monthly 154 (1), 159-169 , 2023 2023 Citations: 2
Polymorphism AR-E211 G> A of the AR Gene (G1733A) In Patients with Prostate Cancer (Pca) In Middle Euphrates of Iraq ZA Abdyaser, AH Alta'ee, MR Joodi HIV Nursing 22 (2), 1851–1848 , 2022 2022
MOST CITED SCHOLAR PUBLICATIONS
Antibacterial Activity of Aluminum Potassium Sulfate and Syzygium Aromaticum Extract Against Pathogenic Microorganisms IA Bnyan, AH Alta'ee, NH Kadhum Journal of Natural Sciences Research 4 (15), 11-15 , 2014 2014 Citations: 46
The Association between Thyroid Hormones and Lipid Profile in Patients with Primary Hyperthyroidism SKAJ Ferdos A. Alterihy, Khawla A. Shemran, Abdulsamie H. Alta'ee Med J Babylon 9 (4), 721-727 , 2012 2012 Citations: 24
Effect of zinc supplementation on urate pathway enzymes in spermatozoa and seminal plasma of Iraqi asthenozoospermic patients: A randomized controlled trial ARS Alsalman, LA Almashhedy, AH Alta'ee, MH Hadwan International journal of fertility & sterility 13 (4), 315 , 2019 2019 Citations: 19
Serum Tumor Necrosis Factor Alpha and Gene Polymorphisms in Rheumatoid Arthritis Patients in Babylon Province, Iraq AK .Alanzy, AH Alta'ee, SJ Alrubiae Journal of Global Pharma Technology 10 (3), 387-395 , 2018 2018 Citations: 18
An efficient protocol for quantifying catalase activity in biological samples MH Hadwan, AH Alta’ee, RM Mohammed, AM Hadwan, HS Al-Kawaz, ... Bulletin of the National Research Centre 48 (1), 34 , 2024 2024 Citations: 17
An optimized protocol for estimating cellulase activity in biological samples ZA Al Talebi, HS Al-Kawaz, RK Mahdi, AT Al-Hassnawi, AH Alta'ee, ... Analytical Biochemistry 655, 114860 , 2022 2022 Citations: 17
Hormonal Contents of Two Types of Black Seed (Nigella sativa) Oil: Comparative Study AH Alta'ee, MJ Ewadh, HK Zaidan Med J Babylon 3 (1-2), 17-21 , 2006 2006 Citations: 15
Role of Some Trace Elements in Breast Cancer Receiving Chemotherapy AH Alta’ee, MJ Ewadh, ZH Kamil International Journal of PharmTech Research 9 (5), 381-386 , 2016 2016 Citations: 14
The Balance Between Superoxide Dismutase and Catalase Activi-ties in Sera of Obese Iraqi Men AH .Alta'ee, MH Hadwan, LA Almashhady Euromediterranean Biomedical Journal 10 (17), 197-202 , 2015 2015 Citations: 14
Novel fluorometric protocol for assessing myeloperoxidase activity R Majid, ZA Al Talebi, HS Al-Kawaz, AH Alta'ee, ARS Alsalman, ... Enzyme and Microbial Technology 171, 110320 , 2023 2023 Citations: 11
A study of arginase-1 activity and lipid profile in patients with myocardial infarction AA Al-Anbari, AH Alta'ee, SF Al-Saad Adv Biotechnol Exp Ther. 5 (3), 553-561 , 2022 2022 Citations: 11
Low levels of peroxiredoxins are associated with high iron content and lipid peroxidation in seminal plasma from asthenozoospermic infertile men LA Almashhedy, HA Fadhil, ARS Alsalman, HS Al-Kawaz, AH Alta'ee, ... Endocrine and Metabolic Science 12, 100137 , 2023 2023 Citations: 9
The Alteration in Parathyroid Hormone and Calcitonin of Pregnant Women in Babylon Governorate HS Hammadi, AH Alta’ee, BA Musa JOURNAL OF BIOMEDICINE AND BIOCHEMISTRY 2 (2), 9–16 , 2023 2023 Citations: 9
A new relationship between cytidine deaminase activity and cancer via oxidative hypothesis AH Alta'ee University of Babylon , 2003 2003 Citations: 9
Effect of Toll-Like Receptor 7 Gene Polymorphism and ABO Blood Groups on the Severity of COVID-19 Patients AHJ Zainab H. D. AL-Tamimi, Abdulsamie H. Alta’ee ACTA INFORMATICA MEDICA 30 (3), 191-195 , 2022 2022 Citations: 7
Leptin and Resistin Induce Oxidative Stress in Patients with Chronic Plaque Psoriatic AM Al-Mokhtar, AH Alta'ee, MK Al-Hattab Res. J. Pharm., Biol. Chem. Sci. 8 (1), 135-142 , 2017 2017 Citations: 7
Impact of lipid-lowering drugs on lipid profile in hyperlipidemia patients KA Shemran, SH Edin, AH Alta’ee Medical Journal of Babylon 20 (2), 274-277 , 2023 2023 Citations: 6
Vitamin D3 level and its receptor of patients with psoriasis: a case control study MAAH Khalil, AH Alta'ee, HA Al-Sultany, MK Al-Hattab EuroMediterranean Biomedical Journal 13 , 2018 2018 Citations: 6
Study of insulin and counter regulatory hormones in patients with type II diabetes mellitus: A case control study IS Kashan, AH Alta’ee, AH Albayati Biochemical and Cellular Archives 18 (1), 77-83 , 2018 2018 Citations: 6
Association of oxidative stress and disease activity in rheumatoid arthritis patients in babylon province BA Aljoboury, AH Alta’ee, SJ Alrubaie Medico Legal Update 20 (2), 565-569 , 2020 2020 Citations: 5