Andreea Iosageanu

Scopus Publications

Corrigendum to ‘4-phenyl isothiocyanate modified electrode based on diazonium electrodeposition for heavy metals detection in pharmaceuticals’(Talanta, (2025), 295, C, (128339), (S003991402500829X), 10.1016/j.talanta.2025.128339)
Maria-Cristina Radulescu, Madalina-Petruta Bucur, Bogdan Bucur, Andreea Iosageanu, Gabriel Lucian Radu
Talanta, 2026
4-Phenyl isothiocyanate modified electrode based on diazonium electrodeposition for heavy metals detection in pharmaceuticals
Maria-Cristina Radulescu, Madalina-Petruta Bucur, Bogdan Bucur, Andreea Iosageanu, Gabriel Lucian Radu
Talanta, 2025
A stable layer of 4-phenyl isothiocyanate (4-PITC) was successfully electrografted on glassy carbon electrode (GCE). The electrode modification process was performed in three sequential steps in a single solution: (1) reduction by chronoamperometry of the nitro groups of 4-NITC to amino moieties (2) in situ reaction of amino with nitrous acid to obtain unstable diazonium functional groups and (3) electrografting by chronopotentiometry of diazonium. A thin optimal layer with minimum surface fouling was obtained. The formation of the organic film was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrode was used for detection of Pb(II) and Fe(II) by differential pulse voltammetry (DPV). Under optimal conditions, the limit of detection 1.2 μg L -1 for Pb(II) and of 2.0 μg L -1 for Fe(II) were achieved. The developed electrode was successfully applied to detect Pb(II) and Fe(II) in injectable iron pharmaceuticals used to treat iron deficiency anemia simultaneous with investigation of contamination by Pb(II).
Anti-Inflammatory and Wound Healing Properties of Different Honey Varieties from Romania and Correlations to Their Composition
Andreea Iosageanu, Laura Mihaela Stefan, Oana Craciunescu, Anisoara Cimpean
Life, 2024
The complex composition of honey plays a crucial role in wound healing, exhibiting varying effects at different stages of the healing process. This study investigated seven honey varieties sourced from different regions of Romania using in vitro experimental models developed in macrophage-like, fibroblast, and keratinocyte cell lines to explore the mechanisms by which honey promoted the healing process. This study assessed the impact of honey on inflammatory cytokine production in macrophage-like cells, cell proliferation and collagen synthesis in fibroblasts, and cell proliferation and migration in keratinocytes. Additionally, correlation analysis was conducted to examine the relationship between honey composition and its biological properties. Honey varieties presented both anti- and pro-inflammatory effects. Moreover, they displayed dose-dependent pro-proliferative effects, stimulating collagen synthesis and cell migration, thereby enhancing the re-epithelialization process. The Pearson coefficient analysis indicated a strong positive correlation between biological activities and phenolic content. Additionally, there was a medium positive correlation with the ascorbic acid content and a medium negative correlation with the glucose content in the different honey varieties. Romanian honey varieties rich in phenolics showed potential in modulating inflammation, proliferation, collagen synthesis, and cell migration, suggesting their suitability for further evaluation and development of innovative dressings for skin tissue regeneration.
In Vitro Wound-Healing Potential of Phenolic and Polysaccharide Extracts of Aloe vera Gel
Andreea Iosageanu, Elena Mihai, Ana-Maria Seciu-Grama, Elena Utoiu, Alexandra Gaspar-Pintiliescu, Florentina Gatea, Anisoara Cimpean, Oana Craciunescu
Journal of Functional Biomaterials, 2024
The present study aimed to conduct a comparative investigation of the biological properties of phenolic and polysaccharide extracts obtained using an ultrasound-assisted technique from Aloe vera gel and their effects on each stage of the wound healing process in in vitro experimental models. HPLC analysis showed that the phenolic extract contained aloin, ferulic, and caffeic acid, as well as quercetin dihydrate, as major compounds. Capillary zone electrophoresis indicated the prevalence of mannose and glucose in the polysaccharide extract. Cell culture testing revealed the anti-inflammatory properties of the phenolic extract at a concentration of 0.25 mg/mL through significant inhibition of pro-inflammatory cytokines—up to 28% TNF-α and 11% IL-8 secretion—in inflamed THP-1-derived macrophages, while a pro-inflammatory effect was observed at 0.5 mg/mL. The phenolic extract induced 18% stimulation of L929 fibroblast proliferation at a concentration of 0.5 mg/mL, enhanced the cell migration rate by 20%, and increased collagen type I synthesis by 18%. Moreover, the phenolic extract exhibited superior antioxidant properties by scavenging free DPPH (IC50 of 2.50 mg/mL) and ABTS (16.47 mM TE/g) radicals, and 46% inhibition of intracellular reactive oxygen species (ROS) production was achieved. The polysaccharide extract demonstrated a greater increase in collagen synthesis up to 25%, as well as antibacterial activity against Staphylococcus aureus with a bacteriostatic effect at 25 mg/mL and a bactericidal one at 50 mg/mL. All these findings indicate that the phenolic extract might be more beneficial in formulations intended for the initial phases of wound healing, such as inflammation and proliferation, while the polysaccharide extract could be more suitable for use during the remodeling stage. Moreover, they might be combined with other biomaterials, acting as efficient dressings with anti-inflammatory, antioxidant, and antibacterial properties for rapid recovery of chronic wounds.
Donkey Gelatin and Keratin Nanofibers Loaded with Antioxidant Agents for Wound Healing Dressings
Maria Râpă, Carmen Gaidau, Laura Mihaela Stefan, Andrada Lazea-Stoyanova, Mariana Daniela Berechet, Andreea Iosageanu, Ecaterina Matei, Virginija Jankauskaitė, Cristian Predescu, Virgilijus Valeika, Aistė Balčiūnaitienė, Snezana Cupara
Gels, 2024
Acute and chronic wounds present a significant healthcare challenge, requiring innovative solutions for effective treatment. The exploitation of natural by-products with advanced cell regeneration potential and plant-based materials, which possess bioactive properties, is an innovative topic in wound management. This study investigates the potential of donkey gelatin and keratin for blending with natural bioactive extracts such as sumac, curcumin, and oak acorn to fabricate antioxidant and antimicrobial nanofibers with accelerated wound healing processes. The fabricated nanofibers possess good in vitro biocompatibility, except for the sumac-based donkey nanofibers, where cell viability significantly dropped to 56.25% (p < 0.05 compared to non-treated cells). The nanofiber dimensions showed structural similarities to human extracellular matrix components, providing an ideal microenvironment for tissue regeneration. The donkey nanofiber-based sumac and curcumin extracts presented a higher dissolution in the first 10 min (74% and 72%). Curcumin extract showed similar antimicrobial and antifungal performances to rivanol, while acorn and sumac extracts demonstrated similar values to each other. In vitro tests performed on murine fibroblast cells demonstrated high migration rates of 89% and 85% after 24 h in the case of acorn and curcumin nanofibers, respectively, underscoring the potential of these nanofibers as versatile platforms for advanced wound care applications.
Natural Polymeric Hydrogels Encapsulating Small Molecules for Diabetic Wound Healing
Elena Iulia Oprita, Andreea Iosageanu, Oana Craciunescu
Gels, 2023
Diabetes is a condition correlated with a high number of diagnosed chronic wounds as a result of a complex pathophysiological mechanism. Diabetic chronic wounds are characterized by disorganized and longer stages, compared to normal wound healing. Natural polymer hydrogels can act as good wound dressings due to their versatile physicochemical properties, represented mainly by high water content and good biocompatibility. Natural bioactive hydrogels are polymers loaded with bioactive compounds providing antibacterial and antioxidant properties, modulation of inflammation and adherence to wounded tissue, compared to traditional dressings, which enables promising future applications for diabetic wound healing. Natural bioactive compounds, such as polyphenols, polysaccharides and proteins have great advantages in promoting chronic wound healing in diabetes due to their antioxidant, anti-inflammatory, antimicrobial, anti-allergic and wound healing properties. The present paper aims to review the wound healing mechanisms underlining the main issues of chronic wounds and those specifically occurring in diabetes. Also, the review highlights the recent state of the art related to the effect of hydrogels enriched with natural bioactive compounds developed as biocompatible functional materials for improving diabetic-related chronic wound healing and providing novel therapeutic strategies that could prevent limb amputation and increase the quality of life in diabetic patients.
In Vitro Hypoglycemic Potential, Antioxidant and Prebiotic Activity after Simulated Digestion of Combined Blueberry Pomace and Chia Seed Extracts
Elena Mihai, Bogdan-Stefan Negreanu-Pirjol, Oana Craciunescu, Teodora Ciucan, Andreea Iosageanu, Ana-Maria Seciu-Grama, Ana-Maria Prelipcean, Elena Utoiu, Viorica Coroiu, Ana-Maria Ghenea, Ticuta Negreanu-Pirjol
Processes, 2023
This study aimed to evaluate the hypoglycemic potential, antioxidant activity and prebiotic activity of a hydroalcoholic extract of blueberry pomace (BP), an aqueous extract of chia seeds (CS) and a novel combination of BP–CS extracts (BCM) for further use as ingredient of functional food. Spectrometric and HPLC analyses were used to characterize the total phenolic and flavonoid content and composition of BP, while CS was analyzed for total carbohydrate content. Data showed that the BCM mixture exerted an inhibition of α-amylase activity, which was 1.36 times higher than that of BP and 1.25 higher than CS extract. The mixture also showed better scavenging activity of free DPPH radicals than individual extracts, and had an IC50 value of 603.12 µg/mL. In vitro testing indicated that both serum- and colon-reaching products of simulated intestinal digestion of BCM presented the capacity to protect Caco-2 intestinal cells against oxidative stress by inhibition of reactive oxygen species production. In addition, the colon-reaching product of BCM digestion had the capacity to significantly (p < 0.05) stimulate the growth of Lactobacillus rhamnosus and Lactobacillus acidophilus, revealing a prebiotic potential. All these results indicated that improved biological activity of the novel combination of BP and CS extracts could be due to the synergistic action of constituents. The combination is recommended for further testing and the development of novel functional food for controlling type 2 diabetes and gastrointestinal conditions.
Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx.) Elliot
Bogdan-Stefan Negreanu-Pirjol, Ovidiu Cristian Oprea, Ticuta Negreanu-Pirjol, Florentina Nicoleta Roncea, Ana-Maria Prelipcean, Oana Craciunescu, Andreea Iosageanu, Victoria Artem, Aurora Ranca, Ludmila Motelica, Anca-Cristina Lepadatu, Madalina Cosma, Dan Razvan Popoviciu
Antioxidants, 2023
Lonicera caerulaea L. and Aronia melanocarpa (Michx.) Elliot fruits are frequently used for their health benefits as they are rich in bioactive compounds. They are recognized as a source of natural and valuable phytonutrients, which makes them a superfood. L. caerulea presents antioxidant activity three to five times higher than other berries which are more commonly consumed, such as blackberries or strawberries. In addition, their ascorbic acid level is the highest among fruits. The species A. melanocarpa is considered one of the richest known sources of antioxidants, surpassing currants, cranberries, blueberries, elderberries, and gooseberries, and contains one of the highest amounts of sorbitol. The non-edible leaves of genus Aronia became more extensively analyzed as a byproduct or waste material due to their high polyphenol, flavonoid, and phenolic acid content, along with a small amount of anthocyanins, which are used as ingredients in nutraceuticals, herbal teas, bio-cosmetics, cosmeceuticals, food and by the pharmaceutical industry. These plants are a rich source of vitamins, tocopherols, folic acid, and carotenoids. However, they remain outside of mainstream fruit consumption, being well known only to a small audience. This review aims to shed light on L. caerulaea and A. melanocarpa and their bioactive compounds as healthy superfoods with antioxidant, anti-inflammatory, antitumor, antimicrobial, and anti-diabetic effects, and hepato-, cardio-, and neuro-protective potential. In this view, we hope to promote their cultivation and processing, increase their commercial availability, and also highlight the ability of these species to be used as potential nutraceutical sources, helpful for human health.
Antibacterial Aloe vera Based Biocompatible Hydrogel for Use in Dermatological Applications
Mariana Chelu, Adina Magdalena Musuc, Ludmila Aricov, Emma Adriana Ozon, Andreea Iosageanu, Laura M. Stefan, Ana-Maria Prelipcean, Monica Popa, Jose Calderon Moreno
International Journal of Molecular Sciences, 2023
The present research aims to describe a new methodology to obtain biocompatible hydrogels based on Aloe vera used for wound healing applications. The properties of two hydrogels (differing in Aloe vera concentration, AV5 and AV10) prepared by an all-green synthesis method from raw, natural, renewable and bioavailable materials such as salicylic acid, allantoin and xanthan gum were investigated. The morphology of the Aloe vera based hydrogel biomaterials was studied by SEM analysis. The rheological properties of the hydrogels, as well as their cell viability, biocompatibility and cytotoxicity, were determined. The antibacterial activity of Aloe vera based hydrogels was evaluated both on Gram-positive, Staphylococcus aureus and on Gram-negative, Pseudomonas aeruginosa strains. The obtained novel green Aloe vera based hydrogels showed good antibacterial properties. In vitro scratch assay demonstrated the capacity of both AV5 and AV10 hydrogels to accelerate cell proliferation and migration and induce closure of a wounded area. A corroboration of all morphological, rheological, cytocompatibility and cell viability results indicates that this Aloe vera based hydrogel may be suitable for wound healing applications.
Progress in Composite Hydrogels and Scaffolds Enriched with Icariin for Osteochondral Defect Healing
Elena Iulia Oprita, Andreea Iosageanu, Oana Craciunescu
Gels, 2022
Osteochondral structure reconstruction by tissue engineering, a challenge in regenerative medicine, requires a scaffold that ensures both articular cartilage and subchondral bone remodeling. Functional hydrogels and scaffolds present a strategy for the controlled delivery of signaling molecules (growth factors and therapeutic drugs) and are considered a promising therapeutic approach. Icariin is a pharmacologically-active small molecule of prenylated flavonol glycoside and the main bioactive flavonoid isolated from Epimedium spp. The in vitro and in vivo testing of icariin showed chondrogenic and ostseoinductive effects, comparable to bone morphogenetic proteins, and suggested its use as an alternative to growth factors, representing a low-cost, promising approach for osteochondral regeneration. This paper reviews the complex structure of the osteochondral tissue, underlining the main aspects of osteochondral defects and those specifically occurring in osteoarthritis. The significance of icariin’s structure and the extraction methods were emphasized. Studies revealing the valuable chondrogenic and osteogenic effects of icariin for osteochondral restoration were also reviewed. The review highlighted th recent state-of-the-art related to hydrogels and scaffolds enriched with icariin developed as biocompatible materials for osteochondral regeneration strategies.
Comparative Palynological, Physicochemical, Antioxidant and Antibacterial Properties of Romanian Honey Varieties for Biomedical Applications
Andreea Iosageanu, Elena Mihai, Ana‐Maria Prelipcean, Ruxandra Elena Anton, Elena Utoiu, Anca Oancea, Oana Craciunescu, Anisoara Cimpean
Chemistry and Biodiversity, 2022
Free Radical Scavenging, Redox Balance and Wound Healing Activity of Bioactive Peptides Derived from Proteinase K-Assisted Hydrolysis of Hypophthalmichthys molitrix Skin Collagen
Daniela Ilie, Andreea Iosageanu, Oana Craciunescu, Ana-Maria Seciu-Grama, Catalina Sanda, Florin Oancea
Food Technology and Biotechnology, 2022
Marine and Agro-Industrial By-Products Valorization Intended for Topical Formulations in Wound Healing Applications
Ana-Maria Prelipcean, Andreea Iosageanu, Alexandra Gaspar-Pintiliescu, Lucia Moldovan, Oana Craciunescu, Ticuta Negreanu-Pirjol, Bogdan Negreanu-Pirjol, Raul-Augustin Mitran, Mariana Marin, Ugo D’Amora
Materials, 2022
Enhanced Wound Healing Activity of Undenatured Type I Collagen Isolated from Discarded Skin of Black Sea Gilthead Bream (Sparus aurata) Conditioned as 3D Porous Dressing
Alexandra Gaspar‐Pintiliescu, Elena Daniela Anton, Andreea Iosageanu, Daniela Berger, Cristian Matei, Raul‐Augustin Mitran, Ticuta Negreanu‐Pirjol, Oana Craciunescu, Lucia Moldovan
Chemistry and Biodiversity, 2021
Effect of fish bone bioactive peptides on oxidative, inflammatory and pigmentation processes triggered by uvb irradiation in skin cells
Andreea Iosageanu, Daniela Ilie, Oana Craciunescu, Ana-Maria Seciu-Grama, Anca Oancea, Otilia Zarnescu, Ionut Moraru, Florin Oancea
Molecules, 2021
Extracellular matrix biomimetic polymeric membranes enriched with silver nanoparticles for wound healing
Laura Mihaela Stefan, Andreea Iosageanu, Daniela Ilie, Ana-Maria Stanciuc, Cristian Matei, Daniela Berger, Oana Craciunescu
Biomedical Materials Bristol, 2021
Tailoring the health-promoting potential of protein hydrolysate derived from fish wastes and flavonoids from yellow onion skins: From binding mechanisms to microencapsulated functional ingredients
Leontina Grigore-Gurgu, Oana Crăciunescu, Iuliana Aprodu, Carmen Alina Bolea, Andreea Iosăgeanu, Brîndușa Alina Petre, Gabriela Elena Bahrim, Anca Oancea, Nicoleta Stănciuc
Biomolecules, 2020

Andreea Iosageanu

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Scopus Publications