Assylbek

EDUCATION

Высшее

RESEARCH, TEACHING, or OTHER INTERESTS

Veterinary, Agricultural and Biological Sciences, General Veterinary

Scopus Publications

Equine Sarcocystosis in the Northern Region of the Republic of Kazakhstan
Zhanaidar Bermukhametov, Kulyay Suleimanova, Oksana Tomaruk, Bakhit Baimenov, Pavel Shevchenko, Assylbek Batyrbekov, Zoja Mikniene, Ahmet Onur Girişgin, Raushan Rychshanova
Animals, 2024
Background: A total of 396 samples were taken from the hearts, oesophagi, and diaphragms of 132 horses slaughtered at slaughterhouses in 2023 for subsequent examination. Methods: The histological method revealed pathomorphological changes in the muscle tissue. The molecular method identified the pathogen species. Results: Histological examination revealed thick-walled cysts with internal septa and numerous bradyzoites, and mononuclear inflammatory cells with pericyst infiltrates. Microcyst samples were amplified by polymerase chain reaction. Molecular genetic analysis allowed for the identification of 18 sarcocysts. Phylogenetic analysis of Sarcocystis isolates revealed three separate clades of Sarcocystis bertrami and two separate clades of Sarcocystis fayeri. Comparison and phylogenetic analysis revealed a very close relationship between the identified Sarcocystis species and other equine Sarcocystis DNA sequences from China and Japan. Based on the results obtained, the epizootic situation and the parasitic level of sarcocystosis contamination of horses in the northern Kazakhstan were determined. Conclusion: This is the first histological and molecular study to identify Sarcocystis spp. isolated from microscopic forms of equine sarcocysts in the northern Kazakhstan. This research will contribute to the fight against the spread of sarcocystosis in the Republic of Kazakhstan and will allow us to develop proposals for improving the mechanisms of sarcocystosis control.
Safe Technologies of Prophylactic Disinfection in the Presence of Animals
Bakhyt Barakhov, Zhaxylyk Myrzabekov, Nurgul Serikbai, Dinara Narbayeva, Gulmira Alpysbayeva, Assylbek Batyrbekov
American Journal of Animal and Veterinary Sciences, 2023
: Prophylactic disinfection in the presence of animals reduces the concentration of microorganisms in the air and thereby prevents various infectious diseases. The study aims to develop a technological mode of prophylactic disinfection in the presence of animals. As part of the disinfection measures, aerosol disinfection, wet disinfection, and foam disinfection were performed. Sanitation procedures used the preparations of Dezinfekt, Penodez, Yodizol, Glutex, and bleach. The study demonstrated that after wet disinfection in the research object with the use of Penodez by the added foam method (combined method), the obtained quantitative indicators when compared to those of the control objects, indicate a 9.7% increase in disinfection effectiveness. With the use of Yodizol in aerosol disinfection in the presence of piglets, the survival rate of microorganisms in the air dropped by 96.3% and on the floor and surfaces by 95.2 and 93.0%, respectively. In the monitoring of the toxic effects of the preparation on the body, no allergic reactions are observed and the level of hemoglobin in the blood was 8.1% higher than in the control group. The experiment demonstrated the highest effectiveness of the combined disinfection method (wet + foam). The use of safe prophylactic disinfection (aerosol) in the presence of animals was found to be expedient. An eco-friendly composition disinfectant based on iodine compounds (Yodizol) was developed for disinfection in the presence of animals. The timing of prophylactic disinfection (considering the accumulation of microorganisms on various surfaces and in the air in facilities) is established.
The influence of lactic acid bacteria on the viability of the reference strain of Listeria monocytogenes 123 serotype I in plant foods
A. Yeleussizova, P. Sobiech, N. Kaumenov, A. Batyrbekov, J. Błażejak-Grabowska, A. Isabaev, A. Platt-Samoraj
Polish Journal of Veterinary Sciences, 2023
Listeria (L.) monocytogenes is the causative agent of human listeriosis, the frequent sourceof which is food of animal origin. The aim of this study was to determine the influence of lactic acid bacteria (LAB) on the viability of Listeria in carrot juice and compound feed inoculated with L. monocytogenes. The effect of homogenous cultures of Streptococcus (Str.) lactis distaticus, Str. thermophilus and Lactobacillus (Lac.) lactis subsp. Cremoris and the combination of Str. thermophilus with Lac. bulgaricus in the carrot juice and compound feed samples on viability of inoculated L. monocytogenes were examined. There were no statistically significant differences in the results between the experimental groups. Regardless of used LAB, the results showed that the mean pH values in the carrot juice samples decreased from an initial pH of 6.7 to a mean value of 3.7 on 15 experimental day. The Listeria concentration in carrot juice samples decreased from average of 4.94 on day 5 to 3.24 log CFU/mL on day 10, and on day 15 achieved <0.01 log CFU/mL. In the compound feed trials, the pH decreased average from initial 6.5 to 3.7 on day 15. The concentration of Listeria decreased, similarly to the carrot juice samples, from average 5.0 on day 5 to 4.68 on day 10, and on day 15 achieved <0.01 log CFU/mL. In control samples, the number of Listeria increased throughout the study period and amounted to 9.2-9.84 log CFU/mL/g in all the samples. The activity of LAB has been shown to be antagonistic to L. monocytogenes. The results of the study did not show any clear differences between the used LAB strains in limiting the L. monocytogenes concentration. Based on the obtained results it can be conducted that the addition of LAB to animal food increases its microbiological safety.