Keagile Bati
@ub.bw
Biomedical Sciences
University of Botswana
EDUCATION
Master of Science; Biological sciences and Biotechnology
RESEARCH, TEACHING, or OTHER INTERESTS
Biochemistry, Genetics and Molecular Biology, Molecular Medicine, Biotechnology, Cancer Research
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Phazha Bushe Baeti, Donald Phenyo Brown, Keagile Bati, G.F. Chi, Ibrahim Demirtaş, Kabo Masisi, Goabaone Gaobotse, and Tebogo Elvis Kwape
Elsevier BV
Nayang A. Kgakatsi, Runner R.T. Majinda, Ishmael B. Masesane, Mutshinyalo S. Nwamadi, Taye B. Demissie, and Keagile Bati
Elsevier BV
Keagile Bati, Phazha B. Baeti, Nayang A. Kgakatsi, Runner R.T. Majinda, Goabaone Gaobotse, and Tebogo E. Kwape
Elsevier BV
James T.P. Matshwele, Sebusi Odisitse, Ofentse Mazimba, Taye B. Demissie, Moses O. Koobotse, Daphne T. Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Mosimanegape Jongman,et al.
Elsevier BV
Keagile Bati, Phazha Bushe Baeti, Goabaone Gaobotse, and Tebogo E. Kwape
Horizon E-Publishing Group
Diabetes, a chronic metabolic disorder with increasing global prevalence, poses a significant public health concern, necessitating the development of safe and effective drugs. This study specifically assessed the inhibitory effects of Euclea natalensis leaf extracts on alpha-amylase through in vitro, in vivo, and in silico methods. The extracts were sequentially obtained using solvents of graded polarity. alpha-amylase inhibition studies were conducted through spectrophotometric methods, while in vivo assessments were performed using a starch tolerance test on rats. Molecular docking was carried out using Autodock 4.2.6, and SwissADME, along with ADMETlab 2.0, were employed to determine the drug-likeness and toxicity properties of the literature-mined compounds. The extracts demonstrated significant in vitro inhibition of alpha-amylase, with the methanol extract exhibiting the highest percentage of inhibition at 27% ± 4.2, followed by hexane and aqueous extracts at 18% ± 2.5 and 18% ± 3.7, respectively. In vivo, the extracts lowered blood glucose levels, with acarbose reducing peak blood glucose levels by 42%, while both the aqueous and methanol extracts reduced it by 19% each after 30 min. The overall glucose-lowering effect, based on the area under the starch tolerance curve, ranked as follows: acarbose > methanol > aqueous > hexane > dichloromethane extract. Molecular docking identified 20(29)-lupene-3 beta-isoferulate C3 as the most promising compound with the lowest binding energy of -11.4 kcal/mol. Molecular dynamics revealed that C3 loses stability as it diverges from the active site. Additionally, while all other compounds passed the Lipinski drug-likeness criteria, 20(29)-lupene-3 beta-isoferulate C3 did not. Therefore, the present study suggests that E. natalensis exhibits antidiabetic properties through the inhibition of alpha-amylase and may serve as a source of potential antidiabetic drug molecules.
Keagile Bati, Phazha Bushe Baeti, Goabaone Gaobotse, and Tebogo Elvis Kwape
Informa UK Limited
James T.P. Matshwele, Sebusi Odisitse, Taye B. Demissie, Moses O. Koobotse, Ofentse Mazimba, Daphne Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Mosimanegape Jongman,et al.
Elsevier BV
James T. P. Matshwele, Mosimanegape Jongman, Taye B. Demissie, Moses O. Koobotse, Ofentse Mazimba, Daphne Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Florence Nareetsile,et al.
Springer Science and Business Media LLC
Phazha Baeti, Keagile Bati, Kabo Masisi, Goabaone Gaobotse, and Tebogo Kwape
Informa UK Limited
ABSTRACT Oxidative stress has been linked to a wide array of health-debilitating diseases. To alleviate oxidative stress, antioxidants especially of plant origin are desired due to their potency and low toxicity. The current study, therefore, evaluated the antioxidant properties and cytoprotective effects of Terminalia prunioides pods. Hexane, chloroform, ethyl acetate and methanol extracts from Terminalia prunioides pods were evaluated for flavonoid and total phenol contents. Their effects on 2, 2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Superoxide dismutase (SOD), Catalase (CAT), reduced glutathione (GSH) and Ferric reducing antioxidant power (FRAP) were evaluated. The extracts were also tested for their cytoprotective effects on HeLa cells against H2O2, using 4-[−3(4-Iodophenyl)-2-(4-nitro-phenyl)-2 H-5-tetrazolio]-1,3-benzene sulfonate (WST-1) assay. The methanol extract possessed 67.8 ± 10.4 mg QE/g and 113.2 ± 7.6 mg GAE/g of total flavonoid (TFC) and total phenolic contents (TPC) respectively. Methanol extracts from Terminalia prunioides pods recorded IC50 values of 0.06 mg/mL, 0.07 mg/mL, and 0.24 mg/mL for DPPH, FRAP and ABTS assays respectively. Catalase (CAT), reduced glutathione (GSH) and Superoxide Dismutase (SOD) activities were significantly increased (p < 0.05) in HeLa cells treated with Terminalia prunioides pods extracts. The current study’'s findings indicate the high antioxidant activity of Terminalia prunioides pods extracts and cytoprotection of HeLa cells from H2O2 induced cell death.
Keagile Bati, Tebogo Elvis Kwape, and Padmaja Chaturvedi
Korean Pharmacopuncture Institute
Objectives: This study aimed to evaluate the hypoglycemic effects of an ethanol extract of Cassia abbreviata (ECA) bark and the possible mechanisms of its action in diabetic albino rats. Methods: ECA was prepared by soaking the powdered plant material in 70% ethanol. It was filtered and made solvent-free by evaporation on a rotary evaporator. Type 2 diabetes was induced in albino rats by injecting 35 mg/kg body weight (bw) of streptozotocin after having fed the rats a high-fat diet for 2 weeks. Diabetic rats were divided into ECA-150, ECA-300 and Metformin (MET)-180 groups, where the numbers are the doses in mg.kg.bw administered to the groups. Normal (NC) and diabetic (DC) controls were given distilled water. The animals had their fasting blood glucose levels and body weights determined every 7 days for 21 days. Oral glucose tolerance tests (OGTTs) were carried out in all animals at the beginning and the end of the experiment. Liver and kidney samples were harvested for glucose 6 phosphatase (G6Pase) and hexokinase activity analyses. Small intestines and diaphragms from normal rats were used for α-glucosidase and glucose uptake studies against the extract. Results: Two doses, 150 and 300 mg/kg bw, significantly reduced the fasting blood glucose levels in diabetic rats and helped them maintain normal body weights. The glucose level in DC rats significantly increased while their body weights decreased. The 150 mg/kg bw dose significantly increased hexokinase and decreased G6Pase activities in the liver and the kidneys. ECA inhibited α-glucosidase activity and promoted glucose uptake in the rats’ hemi-diaphragms. Conclusion: This study revealed that ECA normalized blood glucose levels and body weights in type 2 diabetic rats. The normalization of the glucose levels may possibly be due to inhibition of α-glucosidase, decreased G6Pase activity, increased hexokinase activity and improved glucose uptake by muscle tissues.