Experience in Dentistry, with an emphasis on Periodontics, Microbiology and Implantology, working mainly on the following topics: in vitro and in vivo evaluation of biomaterials, titanium surfaces, evaluation of the effects of antimicrobial photodynamic therapy and effects of laser on soft and bone tissue.
EDUCATION
Adjunct Professor IV of the Department of Stomatology and collaborator of the Postgraduate Program in Dentistry at the Federal University of Paraná. He holds a degree in Dentistry from the State University of Ponta Grossa - UEPG (2001), a specialization in Periodontics from ABO-PG (2004), and a Master's degree in Periodontics from the University of São Paulo - FORP-USP (2006). He holds a PhD in Dentistry from the State University of São Paulo - FOAr-UNESP (2011) with a Sandwich PhD from Malmö Högskola - Sweden, under the supervision of Prof. Ann Wennerberg. He has experience in Dentistry, with an emphasis on Periodontics, Microbiology and Implantology, working mainly on the following topics: in vitro and in vivo evaluation of biomaterials, titanium surfaces, evaluation of the effects of antimicrobial photodynamic therapy and effects of laser on soft and bone tissue.
RESEARCH, TEACHING, or OTHER INTERESTS
Dentistry, Periodontics
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Scopus Publications
Scopus Publications
Physical and chemical evaluation of titanium surfaces with nanotopography using mechanical decontamination methods: in vitro study Viviane Maria RANKEL, Nelson Luiz de MACEDO-JÚNIOR, Edson GRACIA-NETO, Caio Vinicius Gonçalves ROMAN-TORRES, Humberto Osvaldo SCHWARTZ-FILHO Revista De Odontologia Da UNESP, 2025 Resumo Introdução Métodos de descontaminação utilizados em implantes podem exercer impacto direto sobre as superfícies de titânio, gerando alterações físico-químicas decorrentes da instrumentação mecânica. Objetivo O objetivo do estudo foi analisar, pelas técnicas de microscopia eletrônica de varredura (MEV) e energia dispersiva de raios X (EDS), a modificação de superfície de titânio, tanto física como quimicamente, após a ação de métodos de instrumentação mecânica preconizados para descontaminação de implantes. Material e método Quinze discos de titânio foram divididos em cinco grupos, tendo três discos cada grupo, conforme a forma de tratamento (grupos: controle, cureta aço inox, cureta de titânio, ultrassom e laser de diodo). Assim, foram aplicados os diferentes métodos e feita a análise de possíveis alterações morfológicas (MEV) e de constituição química (EDS) nas amostras. Resultado Em relação à morfologia, comparando-se ao grupo controle, os resultados mostraram uma alteração mais significativa no grupo em que o ultrassom foi utilizado. As curetas de titânio causaram um desgaste maior sobre as superfícies quando comparadas às curetas aço inox. Quimicamente, notou-se que o percentual de presença de elementos como Ti, O, C, F, Fe, Cr, Al e Si podem variar de acordo com os instrumentos aplicados nas superfícies. Os discos instrumentados com ultrassom apresentaram uma diminuição nos valores de Ti, aumento de O e geraram uma deposição de Fe e Cr. O uso de cureta de aço inox, cureta de titânio e a aplicação de laser não apresentaram diferenças significantes quando comparados ao controle. Conclusão Conclui-se que as diferentes formas de instrumentação mecânica avaliadas causam diferentes alterações, podendo modificar física e quimicamente as superfícies de titânio após sua aplicação.
Evaluation of lip diseases in peddlers on two beaches of Guarujá/Brazil: An observational study Caio Vinicius G. Roman-Torres, Edson Gracia Neto, Angelica Castro Pimentel, Humberto Oswaldo Schwartz-Filho, Wilson Roberto Sendyk, et al. Brazilian Dental Journal, 2024 Sun exposure is responsible for most lip lesions and, when diagnosed late, the prognosis is unfavorable. Treatments are usually mutilating, palliative, and expensive. This study aimed to evaluate the prevalence of lip diseases and factors associated with its development in peddlers on two beaches of the Guarujá/Brazil coast. Data were collected by clinical examination and use of a questionnaire of 182 peddlers on the beaches. They also received an explanatory folder with information about oral care, and the importance of the use of sunscreen for cancer prevention. All workers diagnosed with lip injuries were referred for dental treatment. The prevalence of lip alterations was 40.7% (n = 70), furthermore, smokers included 27.5%, and alcohol consumption was 20.3%. Regarding selfie protection, 72% of workers reported wearing a hat or cap frequently, 48.4% reported using body sunscreen, and only 11.5% reported using lip balm. There were no significant differences in factors such as alcoholism and smoking related to lip lesions diagnosis. However, when characterizing these factors, 48.6% of those who drink, or smoke have lip alterations. It can be concluded that the population of peddlers on the beaches of Guarujá has a high rate of lip alteration which suggests the need to implement educational health strategies aimed at informing the population about risk factors and preventive measures for lip diseases.
Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies Humberto Osvaldo SCHWARTZ-FILHO, Tauane Ramaldes MARTINS, Paulo Roberto SANO, Marcela Takemoto ARAÚJO, Daniel Cheuk Hong CHAN, et al. Brazilian Oral Research, 2024 The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey’s post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.
Demineralized human dentin matrix for alveolar ridge preservation using a volumetric and histologic analyses in rats Gabriela Fracasso Moraes, Rodrigo de Oliveira Caetano, Fernanda Harumi Oku Prochnow, Yasmine Mendes Pupo, Juliana Lucena Schussel, et al. Brazilian Dental Journal, 2022 The aim of this study was to evaluate a Demineralized Human Dentine Matrix (DHDM) as viable biomaterial for alveolar ridge preservation in a rat model. Wistar rats were submitted to the extraction of maxillary first molars bilaterally. Sockets were filled with biomaterials and divided into 4 experimental groups (n=5): blood clot, autogenous bone, bovine-derived xenograft (BDX) and DHDM. Animals were sacrificed at 7, 14 e 28 days. Microtomography (uCT) volumetric evaluation and qualitative histological analyses were performed. Results obtained through the uCT showed similar values between the DHDM and the other experimental groups. The histological evaluation demonstrated DHDM with an unspecific inflammatory process and bone neoformation with slow reabsorption of the material. This result indicates that DHDM implanted in rat sockets is biocompatible and reduces the alveolar ridge volume loss after tooth extraction.
Physical and chemical analysis of the titanium surface after chemical treatment of decontamination with chlorexidine: in vitro study Eduardo Henrique de Souza OLIVEIRA, Marcelo Yudi SAKAMOTO, Giovana CARNEIRO, Viviane Maria RANKEL, Geisla Mary Silva SOARES, et al. Revista De Odontologia Da UNESP, 2020 Resumo Introdução Tratamentos têm sido propostos para a peri-implantite com o objetivo de descontaminar a superfície dos implantes, removendo microrganismos que podem estar associados à doença. Objetivo O objetivo deste estudo foi avaliar a ação in vitro de diferentes métodos de aplicação de digluconato de clorexidina (CLX) na descontaminação de discos de titânio (Ti) com microtopografia e seu efeito físico-químico sobre a superfície. Material e método Vinte discos de Ti foram expostos a inóculo de Escherichia coli por 24 horas. Foram distribuídos em quatro grupos de descontaminação (n=5): 1 - um minuto de exposição à solução de CLX 0,12%; 2 - dois minutos de exposição à solução de CLX 0,12%; 3 - esfregaço durante um minuto com gel de CLX 1%; 4 - esfregaço durante um minuto com gel de CLX 2%. O produto de cada disco foi diluído e plaqueado individualmente. Após 24 horas, realizou-se contagem das unidades de colônias formadas (UFC). Resultado O grupo com o menor número de crescimento de UFC foi o grupo 4 (0,20±0,37), com apenas UFC em uma das amostras. Seguido do grupo 2 (0,40±0,73), grupo 1 (18,60±33,96). O grupo 3 apresentou as maiores quantidades de UFC (36,07±41,39). Em todas as amostras, foi possível observar uma diminuição estatisticamente significante da concentração superficial de Ti, assim como um aumento de Oxigênio. Conclusão Pode-se concluir que o uso de CLX gel a 2% em superfícies de Ti com microtopografia contaminadas com E. coli propicia a eliminação das UFC e que sua aplicação resulta em diminuição do percentual de Ti e aumento do teor de O.
Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study Jaime S. Marques Filho, Jorge Gobara, Gustavo Vargas da Silva Salomao, Laura M. Sumita, Jamil A. Shibli, et al. Mediators of Inflammation, 2018 This study evaluated the presence of cytokines (IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, MIP-1β, and TNF-α) and human herpesvirus (HSV1, HSV2, EBV, CMV, VZV, HHV6, HHV7, and HHV8) in saliva samples taken from subjects with and without peri-implantitis. Forty-two periodontally healthy subjects were divided according to peri-implant condition: healthy and peri-implantitis groups. The clinical parameters as probing depth, clinical attachment level, plaque index, gingival bleeding, bleeding on probing, and suppuration were evaluated. For cytokine detection, multiplex analysis was performed, and PCR assay was used to identify herpesviruses. No significant differences were found in cytokine levels between groups (p>0.05). The presence of herpesvirus was 1.97-fold higher in patients with peri-implantitis (odds ratio, CI 0.52–7.49). The association of the presence or absence of herpesvirus with the salivary markers was statistically significant for MIP-1β(p=0.0087) and TNF-α(p=0.0437) only in the peri-implantitis group. The presence of herpesviruses in patients with peri-implantitis suggests the development of a proinflammatory environment, which is characterized by increased expression of MIP-1βand TNF-αin saliva.
Changes in alveolar bone support induced by the herbst appliance: A tomographic evaluation João Paulo Schwartz, Taisa Boamorte Raveli, Humberto Osvaldo Schwartz-Filho, Dirceu Barnabé Raveli Dental Press Journal of Orthodontics, 2016 Objective: This study evaluated alveolar bone loss around mandibular incisors, induced by the Herbst appliance. Methods: The sample consisted of 23 patients (11 men, 12 women; mean age of 15.76 ± 1.75 years), Class II, Division 1 malocclusion, treated with the Herbst appliance. CBCT scans were obtained before treatment (T0) and after Herbst treatment (T1). Vertical alveolar bone level and alveolar bone thickness of mandibular incisors were assessed. Buccal (B), lingual (L) and total (T) bone thicknesses were assessed at crestal (1), midroot (2) and apical (3) levels of mandibular incisors. Student's t-test and Wilcoxon t-test were used to compare dependent samples in parametric and nonparametric cases, respectively. Pearson's and Spearman's rank correlation analyses were performed to determine the relationship of changes in alveolar bone thickness. Results were considered at a significance level of 5%. Results: Mandibular incisors showed no statistical significance for vertical alveolar bone level. Alveolar bone thickness of mandibular incisors significantly reduced after treatment at B1, B2, B3, T1 and significantly increased at L2. The magnitude of the statistically significant changes was less than 0.2 mm. The changes in alveolar bone thickness showed no statistical significance with incisor inclination degree. Conclusions: CBCT scans showed an association between the Herbst appliance and alveolar bone loss on the buccal surface of mandibular incisors; however, without clinical significance.
Cone beam computed tomography study of apical root resorption induced by herbst appliance João Paulo SCHWARTZ, Taísa Boamorte RAVELI, Kélei Cristina de Mathias ALMEIDA, Humberto Osvaldo SCHWARTZ-FILHO, Dirceu Barnabé RAVELI Journal of Applied Oral Science, 2015 Objective This study evaluated the frequency of root resorption during the orthodontic treatment with Herbst appliance by Cone Beam Computed Tomography (CBCT). Material and Methods The sample comprised 23 patients (11 men, 12 women; mean ages 15.76±1.75 years) with Class II division 1 malocclusion, treated with Herbst appliance. CBCT was obtained before treatment (T0) and after Herbst treatment (T1). All the dental roots, except third molars, were evaluated, and apical root resorption was determined using the axial guided navigation method. Paired t-tests and Wilcoxon T Test were used to compare the dependent samples in parametric and nonparametric cases, respectively. Chi-Square Test with Yates’ correction was used to evaluate the relationship between apical root resorption and gender. Results were considered at a significance level of 5%. Results Apical resorption was detected by CBCT in 57.96% of 980 roots that underwent Herbst appliance treatment. All patients had minimal resorption and there was no statistical significance between the genders. Conclusion CBCT three-dimensional evaluation showed association between Herbst appliance and minimal apical root resorption, mostly in the anchoring teeth, without clinical significance.
Statins and antimicrobial effects: Simvastatin as a potential drug against Staphylococcus aureus biofilm Talita Signoreti Graziano, Maria Claudia Cuzzullin, Gilson Cesar Franco, Humberto Osvaldo Schwartz-Filho, Eduardo Dias de Andrade, et al. Plos One, 2015 Statins are important lipid-lowering agents with other pleiotropic effects. Several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of many infectious diseases. Staphylococcus aureus is one of the main pathogens implicated in nosocomial infections; its ability to form biofilms makes treatment difficult. The present study observed the MIC of atorvastatin, pravastatin and simvastatin against S. aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis. Simvastatin was the only agent with activity against clinical isolates and reference strains of methicilin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). Thus, the effects of simvastatin on the growth, viability and biofilm formation of S. aureus were tested. In addition, a possible synergistic effect between simvastatin and vancomycin was evaluated. Simvastatin’s MIC was 15.65 µg/mL for S. aureus 29213 and 31.25 µg/mL for the other strains of S. aureus. The effect of simvastatin was bactericidal at 4xMIC and bacteriostatic at the MIC concentration. No synergistic effect was found between simvastatin and vancomycin. However, the results obtained against S. aureus biofilms showed that, in addition to inhibiting adhesion and biofilm formation at concentrations from 1/16xMIC to 4xMIC, simvastatin was also able to act against mature biofilms, reducing cell viability and extra-polysaccharide production. In conclusion, simvastatin showed pronounced antimicrobial activity against S. aureus biofilms, reducing their formation and viability.
