KOUADJO CLAUDE GHISLAINE
@cnra.ci
Central Laboratory of Biotechnologie
National Center for Agronomic Research
RESEARCH INTERESTS
Focuses on the use of molecular tools in agricultural production. The objective is the use of modern methods, of biotechnology, for the identification, the characterization of different plant pathogens (viruses, fungi, bacteria, etc.), and the establishment of effective control methods.
Scopus Publications
Scopus Publications
Thulani P. Makhalanyane, Oliver K. I. Bezuidt, Rian E. Pierneef, Eshchar Mizrachi, Adolphe Zeze, Romain K. Fossou, Claude Ghislaine Kouadjo, Samuel Duodu, Chioma B. Chikere, Olubukola O. Babalola,et al.
Springer Science and Business Media LLC
Guy-Pacome T. Touré, Armand W. Koné, Jacob Nandjui, Anicet E. T. Ebou, Abigael N. Otinga, Assoumane A. Maïga, Claude G. Z. Kouadjo, Seydou Tiho, and Adolphe Zézé
Springer Science and Business Media LLC
Chiguié Estelle Raïssa Amon, Romain Kouakou Fossou, Anicet E. T. Ebou, Dominiqueua K. Koua, Claude Ghislaine Kouadjo, Yao Casimir Brou, Don Rodrigue Rosin Voko Bi, Don A. Cowan, and Adolphe Zézé
Frontiers Media SA
The growing understanding that soil bacteria play a critical role in ecosystem servicing has led to a number of large-scale biogeographical surveys of soil microbial diversity. However, most of such studies have focused on northern hemisphere regions and little is known of either the detailed structure or function of soil microbiomes of sub-Saharan African countries. In this paper, we report the use of high-throughput amplicon sequencing analyses to investigate the biogeography of soil bacteria in soils of Côte d’Ivoire. 45 surface soil samples were collected from Côte d’Ivoire, representing all major biomes, and bacterial community composition was assessed by targeting the V4-V5 hypervariable region of the 16S ribosomal RNA gene. Causative relationships of both soil physicochemical properties and climatic data on bacterial community structure were infered. 48 phyla, 92 classes, 152 orders, 356 families, and 1,234 genera of bacteria were identified. The core bacteriobiome consisted of 10 genera ranked in the following order of total abundance: Gp6, Gaiella, Spartobacteria_genera_incertae_sedis, WPS-1_genera_incertae_sedis, Gp4, Rhodoplanes, Pseudorhodoplanes, Bradyrhizobium, Subdivision3_genera_incertae_sedis, and Gp3. Some of these genera, including Gp4 and WPS-1_genera_incertae_sedis, were unequally distributed between forest and savannah areas while other taxa (Bradyrhizobium and Rhodoplanes) were consistently found in all biomes. The distribution of the core genera, together with the 10 major phyla, was influenced by several environmental factors, including latitude, pH, Al and K. The main pattern of distribution that was observed for the core bacteriobiome was the vegetation-independent distribution scheme. In terms of predicted functions, all core bacterial taxa were involved in assimilatory sulfate reduction, while atmospheric dinitrogen (N2) reduction was only associated with the genus Bradyrhizobium. This work, which is one of the first such study to be undertaken at this scale in Côte d’Ivoire, provides insights into the distribution of bacterial taxa in Côte d’Ivoire soils, and the findings may serve as biological indicator for land management in Côte d’Ivoire.
Sara Laetitia Elphège Gnangui, Romain Kouakou Fossou, Anicet Ebou, Chiguié Estelle Raïssa Amon, Dominique Kadio Koua, Claude Ghislaine Zaka Kouadjo, Don A. Cowan, and Adolphe Zézé
MDPI AG
Over the past decade, many projects have been initiated worldwide to decipher the composition and function of the soil microbiome, including the African Soil Microbiome (AfSM) project that aims at providing new insights into the presence and distribution of key groups of soil bacteria from across the African continent. In this national study, carried out under the auspices of the AfSM project, we assessed the taxonomy, diversity and distribution of rhizobial genera in soils from the tropical savannah zones in Northern Côte d’Ivoire. Genomic DNA extracted from seven sampled soils was analyzed by sequencing the V4-V5 variable region of the 16S rDNA using Illumina’s MiSeq platform. Subsequent bioinformatic and phylogenetic analyses showed that these soils harbored 12 out of 18 genera of Proteobacteria harboring rhizobia species validly published to date and revealed for the first time that the Bradyrhizobium genus dominates in tropical savannah soils, together with Microvirga and Paraburkholderia. In silico comparisons of different 16S rRNA gene variable regions suggested that the V5-V7 region could be suitable for differentiating rhizobia at the genus level, possibly replacing the use of the V4-V5 region. These data could serve as indicators for future rhizobial microbiome explorations and for land-use decision-making.
Kouadjo
Academic Journals
In order to study the microbiology of chromium tolerance and reduction at a fly ash dumping site in South Africa, 15 core samples were investigated. It was shown that the 30 year old dumping site exhibited high concentrations of Cr (VI) ranging from 1.6 to 9.6 mg/g. From this contaminated fly ash dumping site, 67 chromium resistant bacteria (CRB) growing in liquid medium in presence of a high chromium concentration (500 μg/ml) were isolated. Full sequencing and phylogenetic analyses of the 16S rRNA gene allowed unambiguous identification of five Staphylococci species distributed nonhomogeneously through the depth profile of the site. There was a discrepancy in the way the five species tolerated chromium at 250 and 500 μg/ml in anaerobic conditions in the presence or absence of nitrate (KNO 3 ). Their potential to reduce chromium under these conditions was also demonstrated. Key words : Fly Ash, chromium contamination, chromium resistant bacteria, Cr (VI) reduction.