Reis-Prado is a MSc and PhD Candidate of Dentistry (area of Endodontics) at the Universidade Federal de Minas Gerais with an interest in regenerative dentistry, drug delivery systems, tissue response to biomaterials and systematic reviews. Currently, Reis-Prado is a research assistant at the University of Michigan School of Dentistry under the supervision of Prof. Marco C. Bottino (Lab of Regenerative Dentistry). Member of the EndoResearch group.
EDUCATION
MSc and PhD student of Dentistry (area of Endodontics) at the Universidade Federal de Minas Gerais School of Dentistry.
Garlic as a novel therapeutic agent for the management of pulp infections: a systematic review Renata de Oliveira Alves, Isabela Maria Passarela Gomes, José Roberto Vergínio de Matos, Alexandre Henrique dos Reis-Prado, Geórgia Rondó Peres, et al. Odontology, 2026 In vitro studies have shown that garlic extracts are effective against microorganisms commonly associated with root canal infections, with the ability to penetrate bacterial biofilms and inhibit the growth of resistant strains. Building upon this preclinical evidence, the objective of the present systematic review was to identify, critically analyze, and synthesize the available clinical trials on the efficacy of garlic as a therapeutic agent in the management of pulp infections. The review was conducted following PRISMA guidelines and registered in PROSPERO, including clinical trials evaluating garlic-based treatments. A literature search was performed, studies were screened, data extracted, and the risk of bias was assessed using the Cochrane tool and the Newcastle–Ottawa Scale. Certainty of evidence was graded with the GRADE approach. Sixteen studies were included, of which 13 evaluated garlic-derived compounds as intracanal medicaments. Clinical success rates were generally high (≥ 90%), with outcomes comparable to formocresol and MTA, and at lower costs. Radiographic success ranged from 55% to 100%, with some studies showing superior results in reducing periapical signs. Microbiological analyses demonstrated a significant short-term reduction in bacterial load, with superior efficacy compared to conventional agents. Three additional studies investigated garlic-based irrigants, reporting moderate-to-high clinical success (around 75%). Overall, the included studies demonstrated a low risk of bias. The certainty of evidence was rated as moderate for randomized controlled trials and low for non-randomized trials. Low-to-moderate certainty evidence suggests garlic-derived formulations may improve clinical and radiographic outcomes when used as irrigants or intracanal medicaments in pulpotomy and pulpectomy procedures in endodontic practice. However, evidence remains limited, and well-designed, standardized clinical trials with long-term follow-up are needed to confirm their efficacy and safety.
Pre-Operative Factors on Prognosis of Regenerative Endodontic Procedures: A Systematic Review and Meta-Analysis Filipe Colombo Vitali, Alexandre Henrique dos Reis‐Prado, Pablo Silveira Santos, Ana Paula Portes Zeno, Patrícia de Andrade de Risso, et al. International Endodontic Journal, 2025 BackgroundAlthough regenerative endodontics has advanced in recent years, the influence of pre‐operative factors on treatment outcomes remains poorly understood.ObjectiveTo evaluate the effect of pre‐operative factors—including age, gender, tooth type, aetiology of pulp necrosis, stage of root development, clinical signs/symptoms and periradicular status—on the treatment outcomes of regenerative endodontic procedures (REPs) in immature permanent teeth.MethodsA literature search was conducted on six electronic databases and grey literature to identify studies investigating the effect of pre‐operative factors on REP outcomes. The risk of bias was assessed using the Newcastle‐Ottawa Scale for observational studies and the Cochrane Risk‐of‐Bias 2 tool for randomised clinical trials (RCTs). Meta‐analyses of proportions were conducted to estimate pooled rates for the outcomes ‘clinical and radiographic healing’, ‘root development’ and ‘apical closure’ across different pre‐operative factors. Associations between these factors and treatment outcomes were assessed through meta‐analyses of effect sizes. The certainty of evidence was evaluated using the GRADE approach.ResultsTwenty studies were included, comprising 13 observational studies and seven RCTs. Most studies presented a moderate to high risk of bias. The pooled success rate for clinical and radiographic healing exceeded 81% across all pre‐operative factors. Moreover, root development was achieved in 52%–95% of cases, whereas complete apical closure ranged from 32% to 91%. No significant association was found between pre‐operative factors and clinical and radiographic healing. It was found that teeth with pulp necrosis due to trauma presented 3.59 times higher relative risk of root development failure compared to those with necrosis due to anatomic anomaly (RR = 3.59, 95% CI 1.21–10.67, p = 0.02). Incisors presented 1.90 times higher relative risk of root development failure (RR = 1.90, 95% CI: 1.37–2.63, p < 0.01) and 1.98 times higher relative risk of incomplete apical closure (RR = 1.98, 95% CI: 1.34–3.13, p = 0.02) compared to premolars. The presence of an apical lesion increased the relative risk of root development failure by 2.55 times (RR = 2.55, 95% CI: 1.63–4.86, p = 0.01). The certainty of evidence was rated as very low.ConclusionPre‐operative factors were not significantly associated with clinical and radiographic healing in REPs. However, trauma‐related pulp necrosis, tooth type (incisors) and the presence of apical lesions were associated with an increased risk of root development failure. These findings should be interpreted with caution due to between‐study heterogeneity, the moderate to high risk of bias and the very low certainty of the evidence.
Survival of dental implants in irradiated head and neck cancer patients compared to non-irradiated patients: An umbrella review Maria Cândida Dourado Pacheco, Alexandre Henrique dos Reis Prado, Danilo Viegas da Costa, Lara Cancella de Arantes, Caio Fernando Teixeira Portela, et al. Plos One, 2025 Background: There is an increasing demand for oral rehabilitation in patients undergoing irradiation for head and neck cancer treatment. Although radiotherapy appears to adversely affect implanted oral rehabilitation, the evidence provided by previous systematic reviews in the field remains controversial. Thus, this umbrella review aimed to evaluate the survival of dental implants installed before and/or after radiotherapy in patients with head and neck cancer compared to those not irradiated. Methods: A comprehensive search of electronic databases was performed in the PubMed, Cochrane Library, Embase, Web of Science, and Google Scholar databases, including manual searches, from inception to February, 2024, with no language or date restrictions. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines were followed. Survival percentage of dental implants was the primary outcome. A Measurement Tool to Assess systematic Reviews (AMSTAR) 2 was used as a critical appraisal tool for the included studies. The protocol for this review has been registered with PROSPERO (CRD42023406059). Results: Of the 1,811 articles screened, 11 articles that evaluated 73,674 implants were included. Quantitative analyses showed 2,674 failures out of 14,471 implants installed in irradiated bone and 1,825 failures out of 34,092 implants in non-irradiated bone. The survival rate was 81.52% for irradiated implants and 94.64% for non-irradiated implants. A significant difference in survival in favor of implants in non-irradiated bone is supported by 11 meta-analyses. The included systematic reviews showed critically low methodological quality. Conclusions: Although the included studies had low methodological quality, the findings indicate a higher failure rate of implants in irradiated patients. Further well-designed clinical studies are warranted.
Garlic as a novel therapeutic agent for the management of pulp infections: a systematic review R de Oliveira Alves, IMP Gomes, JRV de Matos, AH dos Reis-Prado, ... Odontology, 1-29 , 2026 2026
Effects of isotretinoin on bone dynamics in the craniomaxillofacial region: a systematic review of preclinical evidence MF Ferreira, GP Nunes, AH Chaves-Neto, AH Reis-Prado, MP Justo, ... European Archives of Paediatric Dentistry 27 (2), 349-366 , 2026 2026
Knowledge, attitudes, and practices of dentists regarding regenerative endodontics: A systematic review and meta-analysis FC Vitali, AH Reis-Prado, PS Santos, M Cardoso, F Benetti, LC Maia, ... The Journal of the American Dental Association , 2026 2026
Efeito da incorporação de agentes à base de cálcio em géis clareadores: revisão sistemática com meta-análise de estudos clínicos e in vitro GP NUNES, R de Oliveira ALVES, GR PERES, MHF RAGGHIANTI, ... Revista de Odontologia da UNESP 54 (Especial), 0-0 , 2026 2026
Advances in 3D Printed Scaffolds for Periodontal Regeneration A Daghrery, IPM Soares, AH dos Reis‑Prado, IJ de Souza Araújo, ... Current Oral Health Reports 13 (1), 1 , 2026 2026 Citations: 5
Pre‐Operative Factors on Prognosis of Regenerative Endodontic Procedures: A Systematic Review and Meta‐Analysis FC Vitali, AH dos Reis‐Prado, PS Santos, APP Zeno, PA de Risso, ... International Endodontic Journal 58 (12), 1814-1834 , 2025 2025 Citations: 3
Biological evaluation of solutions from bioglass, bioglass modified with cobalt, and calcium hydroxide JR Amadeu, IJP Silva, J Goto, AH REIS-PRADO, KS Caiaffa, MC Crovace, ... Brazilian Oral Research 39, e114 , 2025 2025
Diabetes Mellitus in Endodontics: A Bibliometric Analysis of the Top 50 Most-Cited Papers AH dos Reis-Prado, KS de Paula, GP Nunes, JJ Segura-Egea, ALR Ruiz, ... Journal of Advances in Medicine and Medical Research 37 (11), 74-103 , 2025 2025
Recent advances in injectable hydrogel biotherapeutics for regenerative dental medicine R Dal‐Fabbro, A Daghrery, C Anselmi, IPM Soares, AH Reis‐Prado, ... Macromolecular Bioscience 25 (10), e00096 , 2025 2025 Citations: 10
Multifunctional bilayer scaffold for dental pulp protection and sustained calcium hydroxide release for mineralized tissue regeneration C Anselmi, IPM Soares, R Dal-Fabbro, S Chang, ABG de Carvalho, ... Biomaterials, 123700 , 2025 2025 Citations: 3
Survival of dental implants in irradiated head and neck cancer patients compared to non-irradiated patients: An umbrella review MCD Pacheco, AHR Prado, DV da Costa, LC de Arantes, CFT Portela, ... PLoS One 20 (9), e0324388 , 2025 2025
3D Printing β-TCP-laden GelMA/Alginate interpenetrating-polymer-network biomaterial inks for bone tissue engineering JR de Souza, M Rahimnejad, IPM Soares, C Anselmi, PHC de Oliveira, ... Bioprinting 49, e00413 , 2025 2025 Citations: 11
Análises de fatores que influenciam o procedimento endodôntico regenerativo: análise bibliométrica e avaliação laboratorial AH dos Reis Prado Universidade Federal de Minas Gerais , 2025 2025
Optimizing methods for regenerative endodontics AH dos Reis-Prado, F Benetti, MC Bottino, R Dal-Fabbro Regenerative Medicine 20 (8), 339-351 , 2025 2025 Citations: 1
EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics AH REIS-PRADO, IJP Silva, J Goto, NES Machado, GF Avelar, ... Brazilian Oral Research 39, e068 , 2025 2025 Citations: 1
Metronidazole-laden silk fibroin methacrylated scaffolds for managing periapical lesions A Silverberg, LM Cardoso, ABG de Carvalho, AH dos Reis-Prado, ... Odontology 113 (3), 930-943 , 2025 2025 Citations: 5
Gelatin methacryloyl advances in regenerative dentistry: A global bibliometric analysis APC Dugaich, A da Silva Barboza, CC Leite, A de Oliveira Rocha, ... Journal of Clinical and Experimental Dentistry 17 (6), e732 , 2025 2025
Electrospun SilkMA/silicate-chlorinated cobalt-doped bioactive glass composite for bone regeneration JR de Souza, C Anselmi, LM Cardoso, LT Kito, AH dos Reis-Prado, ... Journal of the Mechanical Behavior of Biomedical Materials 164, 106929 , 2025 2025 Citations: 8
Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics AH dos Reis-Prado, M Rahimnejad, R Dal-Fabbro, PTA de Toledo, ... Bioactive Materials 46, 406-422 , 2025 2025 Citations: 26
Bioactive glasses for bone tissue engineering: a bibliometric study of the top 100-most cited papers AH dos Reis-Prado, JR de Souza, E de Sousa Trichês, ... Odontology 113 (2), 488-530 , 2025 2025 Citations: 5
MOST CITED SCHOLAR PUBLICATIONS
Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review AH dos Reis‐Prado, LG Abreu, RR Fagundes, SC Oliveira, MC Bottino, ... International Endodontic Journal 55 (6), 579-612 , 2022 2022 Citations: 46
Comparison between immediate and delayed post space preparations: a systematic review and meta-analysis AH dos Reis-Prado, LG Abreu, WLF Tavares, IFC Peixoto, ACD Viana, ... Clinical oral investigations 25 (2), 417-440 , 2021 2021 Citations: 28
Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics AH dos Reis-Prado, M Rahimnejad, R Dal-Fabbro, PTA de Toledo, ... Bioactive Materials 46, 406-422 , 2025 2025 Citations: 26
Top 100 most‐cited scientific articles in regenerative endodontics 2019–2023: a bibliometric analysis AH dos Reis‐Prado, CA Maia, GP Nunes, LC de Arantes, LG Abreu, ... International Endodontic Journal 57 (10), 1434-1452 , 2024 2024 Citations: 24
Influence of dental bleaching on the pulp tissue: A systematic review of in vivo studies MV Donato, AH dos Reis‐Prado, LG Abreu, LC de Arantes, J Goto, ... International Endodontic Journal 57 (6), 630-654 , 2024 2024 Citations: 23
Effects of diabetes mellitus on dental pulp: A systematic review of in vivo and in vitro studies RMN Pimenta, AH dos Reis‐Prado, S de Castro Oliveira, J Goto, ... Oral diseases 30 (2), 100-115 , 2024 2024 Citations: 21
Influence of sodium hypochlorite on cyclic fatigue resistance of nickel–titanium instruments: A systematic review and meta-analysis of in vitro studies AH dos Reis-Prado, LG Abreu, LC de Arantes, K dos Santos de Paula, ... Clinical oral investigations 27 (11), 6291-6319 , 2023 2023 Citations: 20
Biological assessment of a new ready-to-use hydraulic sealer F Benetti, JE Gomes-Filho, IO de Azevedo-Queiroz, M Carminatti, ... Restorative dentistry & endodontics 46 (2) , 2021 2021 Citations: 18
Effectiveness and safety of rotary and reciprocating kinematics for retreatment of curved root canals: a systematic review of in vitro studies LP Simões, AH dos Reis-Prado, CRE Bueno, ACD Viana, MAH Duarte, ... Restorative Dentistry & Endodontics 47 (2) , 2022 2022 Citations: 17
Effects of quercetin on mineralized dental tissues: A scoping review GP Nunes, R de Oliveira Alves, MHF Ragghianti, AH dos Reis-Prado, ... Archives of Oral Biology 169, 106119 , 2025 2025 Citations: 16
Biocompatibility, biomineralization, and maturation of collagen by RTR®, bioglass and DM bone® materials F Benetti, CRE Bueno, AH Reis-Prado, MT Souza, J Goto, JMP Camargo, ... Brazilian dental journal 31 (5), 477-484 , 2020 2020 Citations: 16
Antimicrobial photodynamic therapy in the nonsurgical treatment of periodontitis in patients with HIV infection: a systematic review and meta-analysis GP Nunes, R de Oliveira Alves, MHF Ragghianti, TP Martins, ... Lasers in Medical Science 39 (1), 155 , 2024 2024 Citations: 13
Citric acid conditioning as an alternative to EDTA for growth factors release and stem cell response in regenerative endodontics: A systematic review of in vitro studies AH dos Reis-Prado, PTA Toledo, GP Nunes, PAV Ferreira, M Rahimnejad, ... Journal of endodontics 50 (2), 129-143 , 2024 2024 Citations: 13
Antimicrobial silk fibroin methacrylated scaffolds for regenerative endodontics R Narayanam, LM Cardoso, AH dos Reis-Prado, ABG de Carvalho, ... Journal of Endodontics 50 (12), 1752-1760. e2 , 2024 2024 Citations: 12
Cytotoxicity, biocompatibility, and calcium deposition capacity of 45S5 bioglass experimental paste and bio-C temp: In vitro and in vivo study using wistar rats F Benetti, PHC de Oliveira, MPB de Andrade, C Cantiga-Silva, ... Journal of Functional Biomaterials 15 (7), 184 , 2024 2024 Citations: 12
3D Printing β-TCP-laden GelMA/Alginate interpenetrating-polymer-network biomaterial inks for bone tissue engineering JR de Souza, M Rahimnejad, IPM Soares, C Anselmi, PHC de Oliveira, ... Bioprinting 49, e00413 , 2025 2025 Citations: 11
Protective role of calcium-based agents in dental bleaching gels: Insights from a systematic review and meta-analysis of clinical and laboratory evidence GP Nunes, R de Oliveira Alves, GR Peres, MHF Ragghianti, ... Clinical Oral Investigations 29 (4), 180 , 2025 2025 Citations: 11
Influence of ethylenediaminetetraacetic acid irrigation on the regenerative endodontic procedure in an immature rat molar model AH dos Reis‐Prado, SC Oliveira, J Goto, GAB Silva, LTA Cintra, ... International Endodontic Journal 56 (1), 69-79 , 2023 2023 Citations: 11
Recent advances in injectable hydrogel biotherapeutics for regenerative dental medicine R Dal‐Fabbro, A Daghrery, C Anselmi, IPM Soares, AH Reis‐Prado, ... Macromolecular Bioscience 25 (10), e00096 , 2025 2025 Citations: 10
Guidance on the assessment of the functionality of biomaterials for periodontal tissue regeneration: Methodologies and testing procedures A Daghrery, R Dal-Fabbro, AH dos Reis-Prado, IJ de Souza Araújo, ... Dental Materials 41 (3), 306-318 , 2025 2025 Citations: 10