Winawati Eka Putri
@unusa.ac.id
Faculty of Medicine
Universitas Nahdlatul Ulama Surabaya
RESEARCH INTERESTS
Dermatolgy, photoaging
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Dian Safira Devi, Winawati Eka Putri, and Rizki Amalia
AIP Publishing
Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Hari Basuki Notobroto, and Cita Rosita Sigit Prakoeswa
DiscoverSys, Inc.
Introduction: UV light is the principal cause of photoaging, a kind of aging. The problem arises because it is less enjoyable. Utilizing the secretome of adipose stem cells (ASC), which is rich in growth factors, could be a novel anti-photoaging therapy. The secretome of ASCs has been extensively examined for their role in wound healing, but their function in photoaging has just lately been investigated. This study looked at changes in epidermal and dermal thickness, expression of MMP-1 and TIMP-1, as well as dermal collagen density to assess the influence of the secretome of ASC in photoaging treatment. Methods: 28 male Wistar rats, 10-12 weeks old, were randomly assigned to one week of acclimation in each of four groups: UV-irradiated group with topical secretome of ASC (Group P1); UV-irradiated group with topical vehicle only (Group P2); only topical secretome of ASC, non-UV-irradiated (Group P3); and non-UV-irradiated and no topical treatment (Group P4) (Group P0). Prior to treating the rats, the UV exposure was administered for six weeks. Then, for a period of four weeks, the secretome ASC was administered as a treatment. Results: The epidermal and dermal thickness were significantly improved after four weeks treatment compared to UV irradiated with topical vehicle only group (p<0.05). The expression of TIMP-1 and dermal collagen density were significantly increased (p<0.05). Conclusion: Secretome of ASC may be a solution for photoaging skin.
Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Hari Basuki Notobroto, and Cita Rosita Sigit Prakoeswa
DiscoverSys, Inc.
Skin barrier before and after topical Adipose Stem Cell-Conditioned Medium (ASC-CM) treatment in photoaging Winawati Eka Putri1,2, Anang Endaryanto3, Damayanti Tinduh4, Fedik Rantam5, Hari Basuki Notobroto6, Cita Rosita Sigit Prakoeswa6* Background: Photoaging is a type of aging mainly caused by ultraviolet irradiation. The skin barrier is affected in photoaging and it will result in coarseness, roughness and dry skin. It is probably because Ultraviolet (UV) affects the extracellular matrix in the skin. Adipose Stem Cell-Conditioned Medium (ASC-CM), which contains many growth factors, becomes a potential agent as a novel anti-photoaging treatment by repairing the skin barrier. This study aims to evaluate the skin barrier function before and after treatment with ASC-CM in photoaging skin by evaluating changes in Transepidermal Water Loss (TEWL) and skinfold thickness. Methods: A true experimental study was conducted among 28 male Wistar rats aged 10-12 weeks. They were acclimated for 1 week and randomly divided into 4 groups: UV irradiated group with topical ASC-CM (Group P1); UV irradiated group with topical vehicle only (Group P2); only topical ASC-CM, non-UV irradiated (Group P3), and non-UV irradiated and no topical treatment (Group P0). The ASC-CM was given twice a day for four weeks and the total irradiance dose for 6 weeks was 4,2 J/ cm2. Data were analyzed using SPSS version 20 for Windows. Results: There was a statistically significant difference of TEWL between pre-and post-treatment in group P3 (p=0.043) and P0 (p=0.018). In addition, a statistically significant difference in skin thickness was also found between pre-and posttreatment in group P2 (p=0.005) and P3 (p=0.003). There was a statistically significant difference in skin thickness posttreatment (p=0.000); however, there was no statistically significant difference of TEWL post-treatment by One-Way ANOVA test (p=0.138). Conclusion: ASC-CM could be a potential option for photoaging skin based on the TEWL and skin thickness evaluation. However, a longer study period was needed to know the further effect of ASC-CM.
Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Medhi Denisa Alinda, and Cita Rosita Sigit Prakoeswa
DiscoverSys, Inc.
Growth factors of hypoxia Freeze-Dried Adipose Stem CellConditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study Winawati Eka Putri1,2, Anang Endaryanto3, Damayanti Tinduh4, Fedik Rantam5, Medhi Denisa Alinda6, Cita Rosita Sigit Prakoeswa6* Background: Adipose Stem Cells (ASC) are multipotent stem cells which mechanism of action is mainly secretion of various paracrine molecules known as secretome or conditioned medium (CM). Adipose Stem Cell-Conditioned Medium (ASC-CM) collects several soluble factors, such as cytokines, chemokines, and Growth Factors (GF) secreted by almost all types of living cells in the extracellular space. The freeze-dried (FD) form of ASC-CM can reduce the instability of the fresh (FR) form of ASCCM. This study aims to compare the GF levels between FD ASC-CM and FR ASC-CM as a comparative study. Methods: ASC-CM was prepared from human adipose tissue. After we got the FR ASC-CM, some of them were changed into an FD form. We measured the several GF levels using ELISA methods in ng/L. Data were presented in mean and standard deviation as well as analyzed using SPSS version 25 for Windows. Results: The average levels of EGF in both FD ASC-CM and FR ASC-CM were 256.80±9.70 ng/L and 290.20±53.20 ng/L, followed by TGF-β (564.20±12.70 ng/L and 633.10±11.40 ng/L), PDGF (2.90±0.19 ng/L and 2.90±0.20 ng/L), and VEGF (711.10±21.70 ng/L and 788.70±204.80 ng/L) respectively. There was a statistically significant difference in TGF-β levels between FD ASC-CM and FR ASC-CM (p=0.002). However, there was no a significant difference in EGF, PDGF, and VEGF levels between FD ASC-CM and FR ASC-CM (p>0.05) Conclusion: There was a statistically significant difference between FD and FR ASC-CM only in TGF-β levels.