Report of a Fish Kill Due to a Dinoflagellate Bloom in Perak and Penang, Malaysia ROZIAWATI MOHD RAZALI, , NURIN IZZATI MUSTAPA, WAN NORHANA MD. NOORDIN, MASAZURAH A. RAHIM, KIENG SOON HII, PO TEEN LIM, CHUI PIN LEAW, HARMAN MUHD-FAROUK, KU KASSIM KU YAACOB Asian Fisheries Science, 2022 A fish kill incident was reported at the marine fish culture areas north of Perak and south of Penang, Malaysia, on 26 May 2020. An investigation was carried out at 10 stations in Kerian, Perak and Seberang Perai Selatan, Penang. Seawater samples were collected to identify microalgae species and determination of dissolved inorganic nutrients. The physical parameters of water such as salinity, pH, temperature and dissolved oxygen were measured in situ while the dissolved inorganic nutrients were analysed spectrophotometrically. The dominant microalga was identified as Margalefidinium fulvescens (Iwataki, Kawami & Matsuoka) Gómez, Richlen & Anderson, 2017, based on the morphological and molecular characterisation of the large subunit ribosomal gene. Long rounded and ellipsoidal cells, 30-43 µm in length, appeared in chains of single, two, four or eight cells. The sulcus was slightly narrow surrounding the cell about one turn, but the cingulum was rather deep, encircling the cell approximately twice, and the chloroplasts were brownish, granular and scattered peripherally. The highest M. fulvescens cell counts were recorded at 6.22 × 105 cells L-1 and 4.61 × 105 cells L−1 in Kerian, Perak and Seberang Perai Selatan, Penang, respectively. The physical parameters of the seawater from the affected sites were within the Malaysian Marine Water Quality Standard (MMWQS) for aquaculture. However, slightly higher levels of nitrate, phosphate and ammonia were noted at several stations. Although the exact cause of the bloom was undecided, it could be due to nutrient discharge along the coasts, which also concurred with the transition phase of the northeast to the southwest monsoon.
Relationship between the carapace width and body weight increments and the confirmation of stage 1 ovary after molting of the immature orange mud crab, Scylla olivacea, (Herbst, 1796) in captivity Songklanakarin Journal of Science and Technology, 2019
Does water salinity affect the level of 17β-estradiol and ovarian physiology of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity? Adnan Amin-Safwan, Harman Muhd-Farouk, Mohd Pauzi Mardhiyyah, Musa Nadirah, Mhd Ikhwanuddin Journal of King Saud University Science, 2019 This study determined the effect of water salinity on the level of 17β-estradiol and the ovarian physiology (histological changes) of Scylla olivacea in captivity. Sixty newly mature crabs with immature ovaries (carapace width > 90.0 mm, Stage 1 ovary) were exposed to three salinity levels (10, 20 and 30 ppt) for 60 days. The level of 17β-estradiol peaked in the early days of each treatment for all salinity levels but decreased as the crabs matured. Stage 4 ovaries were most produced in the 20 ppt treatment, followed by the 10 ppt treatment, and then the 30 ppt treatment. No significant difference was found for the level of 17β-estradiol between salinity levels, but the oocyte size was observed to be significantly different between salinity levels. Meanwhile, there was a significant negative correlation between oocyte diameter size and the 17β-estradiol level. The study concludes that salinity can affect ovarian maturation but not 17β-estradiol levels.
Dataset on reproductive status of ovary mud crab at different salinity levels Adnan Amin-Safwan, Mohd Pauzi Mardhiyyah, Mohd Affendi Izzah-Syafiah, Harman Muhd-Farouk, Hidayah Manan, Hairul Hafiz Mahsol, Musa Nadirah, Mhd Ikhwanuddin Data in Brief, 2019 This article investigated how crabs responded to different culture salinities through ovarian maturation stages using combination of external morphology (ovarian coloration and gonadosomatic index), and histological assessment (oocyte structures and diameter sizes). A total of sixty immature crabs were sampled from coastal water of Setiu Wetlands, Kuala Nerus, Terengganu, Peninsular Malaysia, and were introduced to limb autotomy technique in order to induce molt. Crabs were reared until successfully molted, and leaves prior to hardened shell, before proceed with salinities acclimatization prior to salinity treatments (10, 20 and 30 ppt). Five crabs were randomly selected every 15 days throughout 60-day of culture (Day 15, 30, 45 and 60) for the assessment. The different between each ovarian maturation stages was recorded based on the color appearances, and Kruskal-Wallis analysis were done between gonadosomatic index and oocyte diameter sizes with different salinity treatments. Part of the data is associated with the recent articles [1], [2] and provided here as raw data of Supplementary materials.
Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 1796) through induction of eyestalk ablation and methyl farnesoate Harman Muhd-Farouk, Huda Abdullah Nurul, Ambok Bolong Abol-Munafi, Mohd Pauzi Mardhiyyah, Nurul Hasyima-Ismail, Hidayah Manan, Siti Nor Fatihah, Adnan Amin-Safwan, Mhd Ikhwanuddin Arab Journal of Basic and Applied Sciences, 2019 Many crustacean species including mud crab, genus Scylla are incapable of natural maturation under captivity, thus putting high pressure on the wild catch. Therefore, to increase the availability of mature broodstocks in captivity, this study determined the effect of Eyestalk Ablation (EA) and Methyl Farnesoate (MF) administration on ovarian maturation stages of the orange mud crab, Scylla olivacea. The study was conducted using a control group (T1) consisting of 95% alcohol (widely used as a chemical solvent), and three treatment groups consisting of: 5 µl/g oral administrated MF in the diet (T2), EA (T3) and a combination of both treatments of MF and EA (T4). The highest percentage of ovarian maturation Stage 4 was found in the T4 treatment (20.8%) compared to the other treatments which were T1 (0%), T2 (8.33%) and T3 (12.5%). Ovarian development of the treated groups (T2, T3, and T4) was significantly different compared to the control group (T1) (p < 0.05). However, there was no significant difference observed in the mean Gonadosomatic Index (GSI) in orally administrated MF (T2) compared to the EA group (T3) (p > 0.05), but it was significantly different when compared to the combination group of MF and EA (T4) (p < 0.05). It can be concluded that the combination method of oral administration of MF through diet and EA (T4) is the best technique for producing mature ovaries.
Comparison on natural diets dietary composition and foregut fullness between sexes of Crenate Swimming Crab, Thalamita crenata (RüPPELL, 1830) From Setiu Wetlands, Terengganu Coastal Waters, Malaysia Journal of Sustainability Science and Management, 2019
Sperm viability and quantity of mud crab, Scylla tranquebarica in different cryoprotectants Aacl Bioflux, 2018
Biological information and size at maturity of male crenate swimming crab, Thalamita crenata from Setiu Wetlands, Terengganu Coastal Waters Journal of Sustainability Science and Management, 2017
Histological characteristics on the testes of mud spiny lobster, Panulirus polyphagus (Herbst, 1793) Siti Nor Fatiha, Harman Muhd-Farou, Adnan Amin-Safwa, Hairul Hafiz Mahs, Mhd Ikhwanuddi Pakistan Journal of Biological Sciences, 2017 BACKGROUND AND OBJECTIVE Mud spiny lobsters, Panulirus polyphagus (P. polyphagus) are one of the most important fisheries resources now-a-days due to quality that it's possessed. However, there is still lack of in-depth study about this species mainly on males testicular characteristics and sexual maturity size. Therefore, the present study was carried out to investigate the histological characteristics on the testes and sexual maturity sizes of mud spiny lobster, Panulirus polyphagus. MATERIALS AND METHODS The testes were dissected out and fixed in 10% buffered formalin solution for 11 h, dehydrated in 70% alcohol and lastly placed in tissue processor for 18±1 h at 60°C. The tissues blocks were cut at the thickness of 4 μm on a rotary microtome. Stained tissues were taken under Advance Microscope (Nikon Eclipse 80i Nomarski DIC). Collected data were analyzed using Microsoft Excel 2013. Data were presented as mean±standard deviation. Statistical analyses were done using one-way ANOVA using SPSS (Version 22). RESULTS These lobules of mature P. polyphagus were formed via different germinative lineage cells such as spermatogonia, spermatocytes, spermatids and spermatozoa. The histological characteristics of testes showed that the process of spermatogenesis went through the stages of four testes maturation which were spermatogonia I and II, spermatocytes I and II, spermatids and spermatozoa stages within different body weight of P. polyphagus. It was found that there were significant difference between body weight and carapace length to the testicular maturation stages (one-way ANOVA and p = 0.000). CONCLUSION The results of this experiment indicated that males P. polyphagus have four stages of testes maturation and can be considered to have fully mature testes that ready for fertilization at 452 g body weight (BW) and 107 mm carapace length (CL) or more.
Study on carapace width growth band counts relationship of orange mud crab, Scylla olivacea (Herbst, 1796) from Terengganu coastal waters, Malaysia N. Hasyima Is, A. Amin Safwa, N. Fairuz Foz, F.H. Megat, H. Muhd Farou, S.A. Kamaruddin, M. Ikhwanuddi, M.A. Ambak Pakistan Journal of Biological Sciences, 2017 BACKGROUND Orange mud crab Scylla olivacea is one of the most important fisheries resources. A new development in ageing technique of crustaceans has been introduced. The detection of growth band deposited in hard structure of gastric mill in the cardiac stomach are found retained after moulting process can be used as age indicator and growth estimation. OBJECTIVE This study was carried out to determine the comparison between carapace width and growth band count of S. olivacea in Malaysia. MATERIALS AND METHODS Samples were collected from Setiu Wetlands, Terengganu, Malaysia from February until August, 2016. Samples were categorized based on their morphological measurements. The mesocardiac and zygocardiac ossicles in the gastric mill of S. olivacea was dissected out and preserved in solutions and underwent a cross sectioning process. A total of 76 of wild S. olivacea ranging from 6.56 to 12.84 cm in carapace width were analysed. The growth band counts were examined for each individual and ranging from 1 to 3 band counts. RESULTS A positive linear relation was observed between CW and GBC with r2 = 0.5178, p<0.01. Overall, there was a strong, positive correlation between CW and GBC. Increase in CW were correlated with increases in GBC respectively for this species. CONCLUSION Therefore, the carapace width, growth band counts and body weight can be used to improve data on growth, recruitment, maturation and mortality. Thus, this study would able to improve new ageing technique and contribute greatly to improve the conservation and management of S. olivacea in Setiu Wetlands, Terengganu, Malaysia.
A Smart Approach to Aquatic Ecosystem Protection: IoT-Based Water Quality Monitoring System (WQMAS) MFH Zainuddin, A. A. ., Nik Nor Muhammad Saifudin Nik Mohd Kamal, Amir ... Malaysian Journal of Science and Advanced Technology (MJSAT) 5 (3), 173–178 , 2025 2025
Influences of Physico-Chemical Water Parameter on The Natural Distribution Site of Cokcles (Tegillarca granosa and Anadara antiquata) in Coastal Areas of Kuala Kedah, Kedah … H Muhd-Farouk, KKK Yaacob, H Harith, MA Hamid, M Ikhwanuddin Agriculture Reports 3 (2), 1-14 , 2024 2024
Moulting performances evaluation of female orange mud crab, Scylla olivacea (Herbst, 1796) in-captivity: effects of water salinity and limb autotomy AS Adnan, LC Gamburud, ISM Affendi, MM Pauzi, HH Mahsol, ... Tropical Life Sciences Research 35 (1), 197 , 2024 2024 Citations: 3
Gonad maturation of giant freshwater prawn, Macrobrahium rosenbergii at the bay of Padas River, Beaufort, Sabah, Malaysia SN Fatihah, AR Hassan, N Fadhilah, MF Harman, M Ikhwanudin Transactions on Science and Technology 9 (4), 177-184 , 2022 2022 Citations: 4
Report of a fish kill due to a dinoflagellate bloom in Perak and Penang, Malaysia RM Razali, NI Mustapa, MA NOORDIN, MA Rahim, KS Hii, PT Lim, ... Asian Fish. Sci 35, 257-268 , 2022 2022 Citations: 7
Effect of mulberry leaves extract (Morus alba) on growth of post larvae in giant freshwater prawn (Macrobrachium rosenbergii) SN Fatihah, H Muhd-Farouk Adv Agri Food Res J 3 (1), a0000296 , 2022 2022 Citations: 2
Effect of substrate on growth, survival and molting in juvenile red claw, Cherax quadricarinatus SN Fatihah, H Muhd-Farouk, NII Raduan, L Leong-Seng, M Ikhwanuddin Preprints , 2020 2020 Citations: 7
EFFECT OF SUBSTRATE ON GROWTH, SURVIVAL AND MOLTING IN JRED CLAW, SN Fatihah, H Muhd-Farouk, NII Raduan, L Leong-Seng, ... 2020
COMPARISON ON NATURAL DIETS DIETARY COMPOSITION AND FOREGUT FULLNESS BETWEEN SEXES OF CRENATE SWIMMING CRAB, Thalamita crenata (RÜPPELL, 1830) FROM SETIU WETLANDS … AS ADNAN, N AZMAN, S KHAIRUL, SYAFIQAH-NURAIN, ... Journal of Sustainability Science and Management 14 (4), 25-37 , 2019 2019 Citations: 2
Relationship between the carapace width and body weight increments and the confirmation of Stage 1 ovary after molting of the immature orange mud crab, Scylla olivacea,(Herbst … A Amin-Safwan, H Muhd-Farouk, N Hasyima-Ismail, HH Mahsol, ... Songklanakarin Journal of Science & Technology 41 (6) , 2019 2019 Citations: 5
Dataset on body weight, carapace width increment and growth band count of mud crabs, Scylla olivacea M Ikhwanuddin, A Amin-Safwan, N Hasyima-Ismail, MN Azra Data in brief 26, 104477 , 2019 2019 Citations: 6
Dataset on reproductive status of ovary mud crab at different salinity levels A Amin-Safwan, MP Mardhiyyah, MA Izzah-Syafiah, H Muhd-Farouk, ... Data in brief 26, 104426 , 2019 2019 Citations: 2
Does water salinity affect the level of 17β-estradiol and ovarian physiology of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity? A Amin-Safwan, H Muhd-Farouk, MP Mardhiyyah, M Nadirah, ... Journal of King Saud University-Science 31 (4), 827-835 , 2019 2019 Citations: 29
Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 1796) through induction of eyestalk ablation and methyl farnesoate H Muhd-Farouk, HA Nurul, AB Abol-Munafi, MP Mardhiyyah, ... Arab Journal of Basic and Applied Sciences 26 (1), 171-181 , 2019 2019 Citations: 27
Determine the sperm quantity and histological characteristics on the testes of male mud crab, Scylla tranquebarica SN Fatihah, N Halik, H Muhd-Farouk Asian Journal of Applied Sciences 12 (4), 173-179 , 2019 2019 Citations: 3
Sperm viability and quantity of mud crab, Scylla tranquebarica in different cryoprotectants SN Fatihah, SC Simon, HH Hashim, H Muhd-Farouk, C Cheng-Ann AACL Bioflux 11 (4), 1098-1106 , 2018 2018 Citations: 5
Biological information and size at maturity of male crenate swimming crab, Thalamita crenata from Setiu Wetlands, Terengganu Coastal Waters H Muhd-Farouk, A Amin-Safwan, MS Arif, M Ikhwanuddin Journal of Sustainability Science and Management 12 (2), 119-127 , 2017 2017 Citations: 16
Histological characteristics on the testes of mud spiny lobster, Panulirus polyphagus (Herbst, 1793). H Muhd-Farouk, A Amin-Safwan, H Hafiz Mahsol, M Ikhwanuddin Pakistan Journal of Biological Sciences: PJBS 20 (7), 365-371 , 2017 2017 Citations: 6
Study on Carapace Width Growth Band Counts Relationship of Orange Mud Crab, Scylla olivacea (Herbst, 1796) from Terengganu Coastal Waters, Malaysia. FH Megat, SA Kamaruddin, M Ikhwanuddin, MA Ambak Pakistan journal of biological sciences: PJBS 20 (3), 140-146 , 2017 2017 Citations: 10
Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus SN Fatihah, S Jasmani, AB Abol-Munafi, S Noorbaiduri, H Muhd-Farouk, ... Aquaculture 462, 56-63 , 2016 2016 Citations: 19
MOST CITED SCHOLAR PUBLICATIONS
Reproducutive Biology on the Gonad of Female Orange Mud Crab, Scylla olivacea (Herbst, 1796) from the West Coastal Water of PeninsularMalaysia M Ikhwanuddin, J Nur-Atika, AB Abol-Munafi, H Muhd-Farouk Internation Journal of Cell Biology , 2014 2014 Citations: 31
Effect of vertebrate steroid hormones on the ovarian maturation stages of orange mud crab, Scylla olivacea (Herbst, 1796) H Muhd-Farouk, S Jasmani, M Ikhwanuddin Aquaculture 451, 78-86 , 2016 2016 Citations: 30
Does water salinity affect the level of 17β-estradiol and ovarian physiology of orange mud crab, Scylla olivacea (Herbst, 1796) in captivity? A Amin-Safwan, H Muhd-Farouk, MP Mardhiyyah, M Nadirah, ... Journal of King Saud University-Science 31 (4), 827-835 , 2019 2019 Citations: 29
Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 1796) through induction of eyestalk ablation and methyl farnesoate H Muhd-Farouk, HA Nurul, AB Abol-Munafi, MP Mardhiyyah, ... Arab Journal of Basic and Applied Sciences 26 (1), 171-181 , 2019 2019 Citations: 27
Effect of Water Salinity on the External Morphology of Ovarian Maturation Stages of Orange Mud Crab, Scylla olivacea (Herbst, 1796) in Captivity. A Amin-Safwan, H Muhd-Farouk, M Nadirah, M Ikhwanuddin Pakistan Journal of Biological Sciences: PJBS 19 (5), 219-226 , 2016 2016 Citations: 20
Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus SN Fatihah, S Jasmani, AB Abol-Munafi, S Noorbaiduri, H Muhd-Farouk, ... Aquaculture 462, 56-63 , 2016 2016 Citations: 19
Biological information and size at maturity of male crenate swimming crab, Thalamita crenata from Setiu Wetlands, Terengganu Coastal Waters H Muhd-Farouk, A Amin-Safwan, MS Arif, M Ikhwanuddin Journal of Sustainability Science and Management 12 (2), 119-127 , 2017 2017 Citations: 16
Effect of steroid hormones 17α-hydroxyprogesterone and 17α-hydroxypregnenolone on ovary external morphology of orange mud crab, Scylla olivacea H Muhd-Farouk, AB Abol-Munafi, S Jasmani, M Ikhwanuddin Asian Journal of Cell Biology 9 (1), 23-28 , 2014 2014 Citations: 15
Sperm Viability Assessment over elapsing time maintained at 2 degrees C of orange mud crab, Scylla olivacea (Herbst, 1796). M Ikhwanuddin, H Muhd-Farouk, AJ Memon, W Wendy, AB Abol-Munafi Pakistan Journal of Biological Sciences: PJBS 17 (9), 1069-1073 , 2014 2014 Citations: 11
Study on Carapace Width Growth Band Counts Relationship of Orange Mud Crab, Scylla olivacea (Herbst, 1796) from Terengganu Coastal Waters, Malaysia. FH Megat, SA Kamaruddin, M Ikhwanuddin, MA Ambak Pakistan journal of biological sciences: PJBS 20 (3), 140-146 , 2017 2017 Citations: 10
Report of a fish kill due to a dinoflagellate bloom in Perak and Penang, Malaysia RM Razali, NI Mustapa, MA NOORDIN, MA Rahim, KS Hii, PT Lim, ... Asian Fish. Sci 35, 257-268 , 2022 2022 Citations: 7
Effect of substrate on growth, survival and molting in juvenile red claw, Cherax quadricarinatus SN Fatihah, H Muhd-Farouk, NII Raduan, L Leong-Seng, M Ikhwanuddin Preprints , 2020 2020 Citations: 7
Dataset on body weight, carapace width increment and growth band count of mud crabs, Scylla olivacea M Ikhwanuddin, A Amin-Safwan, N Hasyima-Ismail, MN Azra Data in brief 26, 104477 , 2019 2019 Citations: 6
Histological characteristics on the testes of mud spiny lobster, Panulirus polyphagus (Herbst, 1793). H Muhd-Farouk, A Amin-Safwan, H Hafiz Mahsol, M Ikhwanuddin Pakistan Journal of Biological Sciences: PJBS 20 (7), 365-371 , 2017 2017 Citations: 6
In vitro fertilization technique in banana shrimp, Fenneropenaeus merguiensis (De Man, 1888) M Ikhwanuddin, AB Noor-Hidayati, NMA Aina-Lyana, H Zulaikha, ... Journal of Fisheries and Aquatic Science 10 (6), 512 , 2015 2015 Citations: 6
Relationship between the carapace width and body weight increments and the confirmation of Stage 1 ovary after molting of the immature orange mud crab, Scylla olivacea,(Herbst … A Amin-Safwan, H Muhd-Farouk, N Hasyima-Ismail, HH Mahsol, ... Songklanakarin Journal of Science & Technology 41 (6) , 2019 2019 Citations: 5
Sperm viability and quantity of mud crab, Scylla tranquebarica in different cryoprotectants SN Fatihah, SC Simon, HH Hashim, H Muhd-Farouk, C Cheng-Ann AACL Bioflux 11 (4), 1098-1106 , 2018 2018 Citations: 5
Gonad maturation of giant freshwater prawn, Macrobrahium rosenbergii at the bay of Padas River, Beaufort, Sabah, Malaysia SN Fatihah, AR Hassan, N Fadhilah, MF Harman, M Ikhwanudin Transactions on Science and Technology 9 (4), 177-184 , 2022 2022 Citations: 4
Moulting performances evaluation of female orange mud crab, Scylla olivacea (Herbst, 1796) in-captivity: effects of water salinity and limb autotomy AS Adnan, LC Gamburud, ISM Affendi, MM Pauzi, HH Mahsol, ... Tropical Life Sciences Research 35 (1), 197 , 2024 2024 Citations: 3
Determine the sperm quantity and histological characteristics on the testes of male mud crab, Scylla tranquebarica SN Fatihah, N Halik, H Muhd-Farouk Asian Journal of Applied Sciences 12 (4), 173-179 , 2019 2019 Citations: 3
