FELIPE PIEDADE GONÇALVES NEVES

@uff.br

Associate Professor, Department of Microbiology and Parasitology, Instituto Biomédico
UNIVERSIDADE FEDERAL FLUMINESE



                 

https://researchid.co/fpgneves

RESEARCH INTERESTS

Bacteriology, Gram-positive cocci, antimicrobial resistance, molecular epidemiology

44

Scopus Publications

781

Scholar Citations

18

Scholar h-index

25

Scholar i10-index

Scopus Publications

  • Detection of Mycoplasma spp. in free-living seabirds
    Samara Gomes de Brito, Thomas Salles Dias, Leandro dos Santos Machado, Jenif Braga de Souza, Nathalie Costa da Cunha, Virginia Léo de Almeida Pereira, Maria Lúcia Barreto, Aloysio de Mello Figueiredo Cerqueira, and Felipe Piedade Gonçalves Neves

    Springer Science and Business Media LLC

  • Antimicrobial Resistance in Streptococcus pneumoniae before and after the Introduction of Pneumococcal Conjugate Vaccines in Brazil: A Systematic Review
    Patricia Alice Knupp-Pereira, Amanda Seabra Cabral, Ítalo Moraes Dolores, Amanda Beiral da Silva, Helvécio Cardoso Correa Póvoa, and Felipe Piedade Gonçalves Neves

    MDPI AG
    Streptococcus pneumoniae causes serious illnesses, such as pneumonia, bacteremia, and meningitis, mainly in immunocompromised individuals and those of extreme ages. Currently, pneumococcal conjugate vaccines (PCVs) are the best allies against pneumococcal diseases. In Brazil, the 10-valent and 13-valent PCVs have been available since 2010, but the threat of antimicrobial resistance persists and has been changing over time. We conducted a systematic review of the literature with works published since 2000, generating a parallel between susceptibility data on isolates recovered from colonization and invasive diseases before and after the implementation of PCVs for routine childhood use in Brazil. This systematic review was based on the Cochrane Handbook for Systematic Reviews of Interventions and Preferred Reporting Items for Systematic Literature Reviews and Meta-Analyses (PRISMA) guidelines. Despite the inclusion of PCVs at a large scale in the national territory, high frequencies of non-susceptibility to important drugs used in pneumococcal diseases are still observed, especially penicillin, as well as increasing resistance to macrolides. However, there are still drugs for which pneumococci have a comprehensive sensitivity profile.

  • First Report of a Wastewater Treatment-Adapted Enterococcus faecalis ST21 Harboring vanA Gene in Brazil
    Beatriz O. Farias, Kaylanne S. Montenegro, Ana Paula A. Nascimento, Mariana Magaldi, Andressa S. Gonçalves-Brito, Claudia Flores, Thais C. Moreira, Felipe P. G. Neves, Kayo Bianco, and Maysa M. Clementino

    Springer Science and Business Media LLC

  • Prevalence, capsular types, antimicrobial resistance and risk factors associated with pneumococcal carriage among children after long-term 10-valent pneumococcal conjugate vaccine use in Brazil
    Letícia B.D.P. Fortuna, Filipe M. Miranda, Isa M.F. Antunes, Amanda B. Silva, Amanda S. Cabral, Ítalo M. Dolores, Nayara T. Cardoso-Marques, Lúcia M. Teixeira, and Felipe P.G. Neves

    Elsevier BV

  • Carriage prevalence, serotype distribution, antimicrobial resistance, pspA typing and pilus islets of Streptococcus pneumoniae isolated from adults living in a Brazilian urban slum
    Amanda Beiral da Silva, Nayara Torres Cardoso-Marques, Ítalo de Moraes Dolores, Lúcia Martins Teixeira, and Felipe Piedade Gonçalves Neves

    Elsevier BV

  • Editorial: Drug resistance in maternal and paediatric bacterial and fungal infections: Is COVID-19 changing the landscape
    Felipe Piedade Gonçalves Neves, Tomislav Mestrovic, and Tatiana Castro Abreu Pinto

    Frontiers Media SA
    COPYRIGHT © 2023 Neves, Mestrovic and Pinto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Editorial: Drug resistance in maternal and paediatric bacterial and fungal infections: Is COVID-19 changing the landscape

  • Streptococcus pneumoniae
    Felipe P. G. Neves and Tatiana C. A. Pinto

    Springer International Publishing

  • Virulence of Lactobacillus spp. misidentified as Enterococcus faecalis from children’s carious dentine
    Gabriela Ceccon Chianca, Lívia Azeredo Alves Antunes, Pâmela Oliveira Ornellas, Felipe Piedade Gonçalves Neves, Helvécio Cardoso Corrêa Póvoa, and Natalia Lopes Pontes Póvoa Iorio

    Informa UK Limited
    Objective: This study aimed to search for Enterococcus faecalis in children's deep carious dentine and characterize their virulence traits.Material and Methods: Eight isolates from 15 carious molars identified by 16S rDNA species-specific PCR as E. faecalis were included. These eight isolates were subject to identification by MALDI-TOF and characterized regarding: (i) bacterial aggregation and biofilm formation on polystyrene and glass, with/without saliva, as single or dual-species (associated to Streptococcus mutans); (ii) environmental pH measurement before and after 24 h incubation; (iii) acidogenicity; (iv) gelatinase production; (v) macrophage adherence; and (vi) toxicity towards Caenorhabditis elegans. Statistical analyses were performed using two-way ANOVA/Tukey or Fisher's exact tests.Results: All isolates initially identified as E. faecalis by PCR were correctly identified as Lactobacillus by MALDI-TOF, being designated as Lactobacillus misidentified as Enterococcus (LME). These isolates produced biofilm in the presence of saliva and in the dual-species assays. Bacterial aggregation was only observed in the dual-species model. After 24 h, environmental pH dropped from 7.5 to 4.5 for seven of eight isolates, and to 4.0 in all dual-species models. LME isolates were acidogenic, none of them produced gelatinase or adhered to macrophages, but all presented toxicity towards C. elegans.Conclusions: No E. faecalis were identified in the children's caries lesions. All LME isolates presented important virulence traits, including biofilm formation and high acidogenicity, which cause enamel demineralization, that might increase the risk of dental caries in children carrying LME. Thus, the correct identification and in-depth virulence characterization of microorganisms isolated from dental caries are important to understand the dynamics of this disease.

  • Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
    Aline R. V. Souza, Sandrine E. C. M. de Pina, Natália S. Costa, Felipe P. G. Neves, Vânia L. C. Merquior, José Mauro Peralta, Tatiana C. A. Pinto, and Lúcia M. Teixeira

    Springer Science and Business Media LLC
    AbstractOptochin susceptibility testing is a major assay used for presumptive identification of Streptococcus pneumoniae. Still, atypical optochin-resistant (Optr) pneumococci have been reported and this phenotype has been attributed to nucleotide substitutions in the genes coding for the F0F1ATPase. While substitutions in the atpC gene (c-subunit of ATPase) are more common and better characterized, data on mutations in the atpA (a-subunit) are still limited. We have characterized five Optr isolates presenting alterations in the atpA (Trp206Cys in four isolates and Trp206Ser in one isolate), constituting the first report of such mutations in Brazil. Most of the Optr isolates consisted of heterogeneous populations. Except for Opt MICs and the nucleotide changes in the atpA gene, Optr and Opts subpopulations originating from the same culture had identical characteristics. In addition, we compared phenotypic and genetic characteristics of these atpA mutants with those of atpC mutants previously identified in Brazil. No structural alterations were detected among predicted proteins, regardless of mutations in the coding gene, suggesting that, despite the occurrence of mutations, protein structures tend to be highly conserved, ensuring their functionalities. Phylogenetic analysis revealed that atypical Optr strains are true pneumococci and Opt resistance does not represent any apparent selective advantage for clinical isolates.

  • Carriage prevalence, serotype distribution, and antimicrobial susceptibility among pneumococcal isolates recovered from adults with systemic lupus erythematosus
    Rodrigo Poubel Vieira de Rezende, Nayara Torres Cardoso-Marques, Larissa Alexandra Silva Rodrigues, João Paulo Chevrand Latini de Almeida, Gecilmara Salviato Pillegi, Lúcia Martins Teixeira, Evandro Mendes Klumb, and Felipe Piedade Gonçalves Neves

    SAGE Publications
    Sir, Patients with systemic lupus erythematosus (SLE) have higher morbidity and mortality due to pneumococcal diseases than the general population. Streptococcus pneumoniae colonization in the upper respiratory tract is a prerequisite for severe pneumococcal diseases such as pneumonia, bacteraemia, and meningitis. Thus, data on the characteristics of the main circulating strains in the community setting (e.g., antimicrobial susceptibility profiles and distribution of serotypes) will be helpful in the management of pneumococcal infections and assessing the potential coverage of commercially available pneumococcal vaccines. Despite limited information on pneumococcal colonization in adults, carriage prevalence among them varies from 1% to 21% (culture-based methods), with no available data on SLE adults. Therefore, we carried out a cross-sectional study to investigate pneumococcal carriage in this population. From June 19, 2018, to January 29, 2019, we recruited 229 SLE patients aged �18 years. The participants were regularly followed up at two university hospitals in Brazil (affiliated to Universidade Federal Fluminense [n1⁄4 49] and Universidade do Estado do Rio de Janeiro [n1⁄4 180]). Nasopharyngeal and oropharyngeal swab specimens were collected in all participants. Specimens were kept at �70�C until culture could be performed. Suspected colonies were identified based on alpha-haemolysis, optochin sensitivity, and bile solubility. Capsular types were determined by multiplex polymerase chain reaction, and antimicrobial susceptibility to nine drugs was evaluated by the disk diffusion method. Minimum inhibitory concentrations of penicillin and erythromycin were determined using M.I.C.Evaluator strips (Oxoid, Basingstoke, Hants, UK). A more in-depth description of these methodologies has been previously published. The patients’ sociodemographic and clinical characteristics are shown in Supplementary table 1. Ten (4.4%) out of 229 participants were pneumococcal carriers, yielding 11 isolates (Table 1). One participant (#155) harboured two distinct strains, and three (#46, #106, #112) had the same strain in both the nasopharynx and oropharynx. Pneumococci were isolated from the oropharynx in 9 out of 10 (90%) carriers and from the nasopharynx in 4 out of 10 (40%) carriers (p1⁄4 0.01; chi-square). Except for one patient, all carriers were judged by their attending rheumatologist to be in clinical remission. None of the studied variables was associated with pneumococcal colonization in the univariate analysis (Supplementary table 1; p> 0.05). As a limitation, accurate data on the patients pneumococcal immunisation records were lacking. Seven different serotypes/serogroups were identified, while four pneumococcal strains were non-typeable (Table 1). Among the 11 isolates, 6 (54.5%) are potentially covered by the 23-valent pneumococcal polysaccharide vaccine (PPV23), and 2 (18.2%) by the 13-valent pneumococcal conjugate vaccine (PCV). All strains were sensitive to chloramphenicol, levofloxacin, rifampicin, and vancomycin. The highest resistance frequencies were observed for penicillin and tetracycline (4/11 strains; 36.4% each). We detected 3 (27.3%) multidrug-resistant (MDR) strains, i.e., resistant to at least three antimicrobial classes. In summary, we showed that 1) the pneumococcal carriage prevalence (4.4%) in our sample of SLE adults was in line with reported colonization frequencies among non-SLE adults; 2) the addition of an oropharynx swab significantly improved the diagnostic yield of S. pneumoniae; 3) MDR pneumococcal strains were not uncommon in the community, and 4) approximately half of the isolates were potentially covered by currently marketed pneumococcal vaccines, especially the PPV23. These findings have implications on clinical practice, reinforcing the need for active epidemiological surveillance and adherence to the recommendations for pneumococcal vaccination of SLE patients. Lupus

  • Biofilm Formation on Breast Implant Surfaces by Major Gram-Positive Bacterial Pathogens
    Gabriel Rezende-Pereira, Julia P Albuquerque, Monica C Souza, Barbara A Nogueira, Marlei G Silva, Raphael Hirata, Ana L Mattos-Guaraldi, Rafael S Duarte, and Felipe P G Neves

    Oxford University Press (OUP)
    Abstract Background Bacterial biofilm on surfaces of mammary implants is a predisposing factor for several outcomes. Because Gram-positive bacteria are potential agents of biomaterial-associated infections (BAIs), their abilities to form biofilm on breast implants should be elucidated. Objectives The aim of this study was to evaluate biofilm formation on different mammary prosthesis surfaces by major Gram-positive bacterial pathogens involved in BAIs. Methods We initially evaluated biofilm formation on polystyrene plates with and without fibrinogen or collagen for 1 reference strain and 1 clinical isolate of Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes. We also tested the ability of clinical isolates to form biofilm on 4 different implant surfaces: polyurethane foam and smooth, microtextured, and standard textured silicone. Biofilm structure and cell viability were observed by scanning electron microscopy and confocal laser scanning microscopy. Results All strains showed strong biofilm formation on polystyrene. After fibrinogen or collagen treatment, biofilm formation varied. With fibrinogen, reference strains of S. aureus and S. pyogenes increased biofilm formation (P < 0.05). Reference strains of all species and the clinical isolate of S. pyogenes increased biofilm formation after collagen treatment (P < 0.05). In general, S. aureus showed higher capacity to produce biofilm. Scanning electron microscopy showed that biofilm attached to all surfaces tested, with the presence of extracellular polymeric substances and voids. Viable cells were more frequent for E. faecalis and S. pyogenes. Conclusions All species produced biofilm on all prosthesis surfaces and under different conditions. Micrographies indicated thicker bacterial biofilm formation on microtextured and/or standard textured silicone by all species, except E. faecalis.

  • Streptococcus pneumoniae prevalence in nasopharynx, oropharynx and gingival sulcus in Brazilian adults:A preliminary study
    P.G. Lomardo, N.T. Cardoso-Marques, L.A. Motta, T.R.S. Aguiar, F.P.G. Neves, F. Aguiar-Alves, M.D. Calasans-Maia, V. Quinelato, and P.L. Casado

    Elsevier BV
    AIM This study aimed to evaluate the prevalence of S. pneumoniae colonization in three different sites in healthy adults: nasopharynx, oropharynx and gingival sulcus. METHODS Two-hundred and sixty five adults, aged 20-60 years, who attended dental clinics in one public university (n = 106) and one military institution (n = 159) were enrolled in this study. Pneumococcal detection was performed by direct culture (DC) and PCR for lytA gene after a broth enrichment step. Capsular types were determined by sequential multiplex PCR. RESULTS We identified 18 (6.8%) pneumococcal carriers among 265 adults by PCR, but only one (0.4%) pneumococcal strain was isolated by DC method. Oropharynx (17; 6.4%) was the main source of S. pneumoniae. Colonization of gingival sulcus and nasopharynx was found in 4 (1.5%) and 2 (0.8%) adults, respectively. Nine distinct capsular types were detected from 9 adults and co-colonization with 2 serotypes was confirmed in 4 (1.5%) subjects. Factors associated with carriage were being females, low level of schooling, non-military and regular medication. We observed a low (6.8%) pneumococcal carriage prevalence, but oropharyngeal samples yielded more sensitive results, especially by the PCR-based detection methodology. CONCLUSION Gingival sulcus was found to be a possible reservoir for S. pneumoniae independently of the oropharynx or nasopharynx colonization.

  • Going online in pandemic time: A DivulgaMicro Workshop Experience
    Laura Maria Andrade de Oliveira, Eric Cordeiro-Spinetti, Felipe Piedade Gonçalves Neves, Patricia Sanae Sujii, Rachel Leite Ribeiro, Sidcley Silva de Lyra, Tatiana Castro Abreu Pinto, and Maria Letícia Bonatelli

    Journal of Microbiology and Biology Education American Society for Microbiology
    DivulgaMicro is a Brazilian Science Communication and Outreach project run by three young female scientists. In 2020, due to the COVID-19 pandemic, we promoted the first virtual edition of the DivulgaMicro Workshop.

  • CRISPR elements and their association with antimicrobial resistance and virulence genes among vancomycin-resistant and vancomycin-susceptible enterococci recovered from human and food sources
    Barbara Araújo dos Santos, Jessica da Silva de Oliveira, Bruna Marcela Parmanhani-da-Silva, Rachel Leite Ribeiro, Lúcia Martins Teixeira, and Felipe Piedade Gonçalves Neves

    Elsevier BV
    We aimed to investigate the occurrence of CRISPR elements in the genomes of vancomycin-resistant (VRE) and vancomycin-susceptible (VSE) enterococci and their association with the presence of antimicrobial resistance and virulence genes. We analyzed 180 isolates, including 91 VRE and 89 VSE. Isolates were identified by PCR or MALDI-TOF. Antimicrobial susceptibility and MICs for vancomycin were determined by the disk-diffusion method and E-test®, respectively. The presence of resistance and virulence genes, as well as CRISPR elements, was investigated by PCR. We identified 95 (53%) E. faecalis, 78 (43%) E. faecium, five (2.8%) E. gallinarum, and one (0.6% each) E. casseliflavus and E. durans. The highest and the lowest non-susceptibility frequencies were observed for erythromycin (n = 152; 84.4%) and fosfomycin (n = 5; 2.8%), respectively. Most erythromycin-resistant isolates had the erm(B) gene (106/152; 69.7%). Of 118 (65.6%) isolates with high-level resistance to aminoglycoside, 69 (58.5%) had at least one aminoglycoside resistance gene, mostly ant(6)-Ia and aac(6')-Ie + aph(2″)-Ia. We found at least one virulence gene among 135 (75%) isolates, mostly gelE (79/180; 43.9%). Ninety-two (51.1%) isolates had at least one CRISPR element, especially CRISPR3 (62/92; 67.4%). CRISPR elements were more common among E. faecalis, in which we observed a relationship between the absence of CRISPR and the presence of the vanA resistance gene, and the hyl and esp virulence genes. Among VRE. faecium, a relationship was found between the absence of CRISPR and the hyl gene. In conclusion, we found evident associations between the lack of CRISPR elements with species, multidrug resistance, and major resistance- and virulence-associated genes.

  • Prevalence of PspA families and pilus islets among Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil
    Patricia Alice Knupp-Pereira, Nayara Torres Cardoso Marques, Lúcia Martins Teixeira, Helvécio Cardoso Corrêa Póvoa, and Felipe Piedade Gonçalves Neves

    Springer Science and Business Media LLC
    In 2010, the 10-valent (PCV10) and 13-valent (PCV13) pneumococcal conjugate vaccines were introduced in Brazil to immunize children, resulting in serotype replacement. We analyzed 253 carriage isolates recovered from children aged <6 years in Brazil, including 124 and 129 isolates from the pre-PCV10/13 (December 2009-July 2010) and post-PCV10/13 (September-December 2014) periods, respectively, to investigate the prevalence of PspA families and pilus islets, potential vaccine candidates. Serotypes and resistance profiles were previously characterized. We used PCR to type PspA families (Fam1-3) and pilus islets (PI-1 and PI-2). We identified the PspA family of 130 (51.4%) isolates. PspA families 1, 2, and 3 were identified in 12.2%, 38.7%, and 0.4% of the isolates, respectively. Eighteen (58.1%) Fam1 isolates were serogroup 6. Nine (81.8%) of 11 serotype 14 isolates were Fam2. Fam1 isolates resistant to penicillin (50%), erythromycin (43.7%), clindamycin (31.2%), and chloramphenicol (6.2%) were only found after PCV10/13 introduction. Resistance among Fam2 isolates was higher in the post-PCV10/13 period to erythromycin (1.8% vs. 18.6%), clindamycin (0 vs. 13.9%), and tetracycline (10.9% vs. 16.3%). PI-I was detected in 42 (16.6%) isolates. Fourteen (56%) of 25 serotype 15B/C and nine (81.8%) of 11 serotype 14 isolates had PI-1 (p < 0.01). Eight (3.2%) isolates had PI-2, and six (75%) were serogroup 19. Five (2%) serogroup 19 isolates had both PI-1 and PI-2. We found associations between serogroups/serotypes, PspA families, and pilus islets, but distribution of PspA families and pilus islets was similar in both periods. After universal vaccination, we observed higher antimicrobial resistance frequencies, regardless PspA or pilus types.

  • Direct effect of the 13-valent pneumococcal conjugate vaccine use on pneumococcal colonization among children in Brazil
    Felipe P.G. Neves, Nayara T. Cardoso, Claudete A.A. Cardoso, Lúcia M. Teixeira, and Lee W. Riley

    Elsevier BV
    BACKGROUND The 13-valent pneumococcal conjugate vaccine (PCV13) has been commercially available in Brazil since 2010. We investigated the carriage prevalence, capsular types, and antimicrobial resistance among pneumococci isolated from children immunized with PCV13 in Brazil. METHODS We analyzed 500 children < 6 years old attending public (n = 270) and private (n = 230) clinics in Niterói/RJ, Brazil, in 2014. We determined the antimicrobial susceptibility and capsular types for all isolates. RESULTS Thirty-eight (7.6%) of 500 children had received at least one PCV13 dose. Since only two (0.7%) of 270 children at the public clinic were vaccinated with PCV13, major analyses focused on 36 (15.7%) of 230 children attending private clinics. Nine (25%) of 36 children were pneumococcal carriers. Characteristics associated with carriage were age ≥ 2 years, cough/expectoration, and childcare center attendance (p ≤ 0.01). The capsular types found were 15B/C (n = 2), 6C, 11A/D, 16F, 23A, and 23F. Two isolates were non-typeable (NT). Three (33.3%) isolates were multidrug resistant. We found four (44.4%) penicillin non-susceptible pneumococci, with penicillin and ceftriaxone MICs ranging from 0.12 to 4.0 µg/ml and 0.023-0.5 µg/ml, respectively. We also detected two (22.2%) erythromycin-resistant isolates (MICs of 3.0 and 256 µg/ml). CONCLUSIONS Colonization with PCV13 serotype was rare among the vaccinated children. Increasing PCV13 coverage might help reduce the frequency of major serotypes currently associated with invasive pneumococcal diseases in Brazil, such as 3 and 19A. The isolation of multidrug-resistant serotype 6C and NT isolates in carriage, however, requires close monitoring.

  • Differences in gram-positive bacterial colonization and antimicrobial resistance among children in a high income inequality setting
    Felipe Piedade Gonçalves Neves, Mariel Asbury Marlow, Gabriel Rezende-Pereira, Marcos Gabriel Pinheiro, Allyne Fandino Martinez dos Santos, Maria de Fátima Nogueira de Freitas, Rosana Rocha Barros, Fábio Aguiar-Alves, Claudete Aparecida Araújo Cardoso, and Lee Woodland Riley

    Springer Science and Business Media LLC
    BackgroundStaphylococcus aureus and beta-hemolytic streptococci (BHS) diseases disproportionately affect populations in middle/low-income countries. To assess if this disparity is reflected in colonization by these organisms, we compared their colonization frequency among children from different socioeconomic status (SES) communities in a city with high income inequality.MethodsBetween May–August 2014, we collected nasal and throat swabs to investigate S. aureus and BHS colonization among children who attended private and public pediatric clinics. Patients were classified as high SES, middle/low SES, and slum residents. We investigated the antimicrobial resistance profile, the SCCmec types and the presence of PVL genes among methicillin-resistant S. aureus (MRSA). We also examined the antimicrobial resistance profile and serogroups of BHS.ResultsOf 598 children, 221 (37%) were colonized with S. aureus, of which 49 (22%) were MRSA. MRSA colonization was higher in middle/low SES (n = 18; 14%) compared with high SES (n = 17; 6%) and slum (n = 14; 8%) residents (p = 0.01). All MRSA strains were susceptible to clindamycin, nitrofurantoin, and rifampin. The highest non-susceptibility frequency (42.9%) was observed to erythromycin. SCCmec type V was only found in isolates from high SES children; types I and II were found only in middle/low SES children. Ten (20%) MRSA isolates carried PVL genes. Twenty-four (4%) children were BHS carriers. All BHS (n = 8) found in high SES children and six (67%) isolates from slum patients belonged to group A. All group B streptococci were from middle/low SES children, corresponding to five (71%) of the seven BHS isolated in this group. BHS isolates were susceptible to all drugs tested.ConclusionsChildren from different SES communities had distinct bacterial colonization profiles, including MRSA carriage. Public health officials/researchers should consider SES when assessing disease transmission and control measures.

  • Evolution of penicillin non-susceptibility among streptococcus pneumoniae isolates recovered from asymptomatic carriage and invasive disease over 25 years in Brazil, 1990–2014
    Tatiana Castro Abreu Pinto, Felipe Piedade Gonçalves Neves, Aline Rosa Vianna Souza, Laura Maria Andrade Oliveira, Natália Silva Costa, Luciana Fundão Souza Castro, Cláudia Rezende de Vieira Mendonça-Souza, José Mauro Peralta, and Lúcia Martins Teixeira

    Frontiers Media SA
    Streptococcus pneumoniae is a major cause of community-acquired pneumonia and meningitis, and it is also found as a commensal, colonizing the human upper respiratory tract of a portion of the human population. Its polysaccharide capsule allows the recognition of more than 90 capsular types and represents the target of the currently available pneumococcal conjugate vaccines (PCVs), such as the 10-valent (PCV10) and the 13-valent (PCV13). Penicillin non-susceptible pneumococci (PNSP) have been listed as one of the current major antimicrobial-resistant pathogen threats. In Brazil, the emergence of PNSP was initially detected in the mid 1990s and PCV10 has been part of the National Immunization Program since 2010. Here, we investigated the distribution of capsular types and penicillin susceptibility profiles of 783 pneumococcal strains isolated in Brazil between 1990 and 2014 to assess the evolution of penicillin non-susceptibility among pneumococci associated with asymptomatic carriage and invasive pneumococcal disease (IPD). The most common serotypes among carriage isolates were 19F, 6B, 6C, 23F, and 14. Among IPD isolates, the most frequent types were 14, 3, 6B, 5, 19F, and 4. We detected 21 types exclusively associated with IPD isolates, whereas non-typeable (NT) isolates were only detected in carriage. Nearly half of the isolates belonged to PCV10 serotypes, which remarkably decreased in occurrence (by nearly 50%) after PCV10 introduction (2011–2014), while non-PCV10 serotypes increased. PNSP frequency and levels were much higher among carriage isolates, but PNSP belonging to PCV10 serotypes were more common in IPD. While the occurrence of PNSP has decreased significantly among IPD isolates since 2011, it kept increasing among carriage strains. Such a difference can be attributed to the serotypes that emerged in each clinical source after PCV10 usage. PNSP with multidrug resistance profiles that emerged within carriage isolates comprised mostly serotypes 6C and 35B, as well as NT isolates. In turn, penicillin-susceptible capsular types 3, 20, and 8 have risen among IPD. Overall, our results reinforce the relevance of PNSP surveillance over a long period of time to better understand the dynamics of antimicrobial resistance in response to PCV introduction and may also contribute to improve control measures toward drug-resistant pneumococci.

  • Population structure of Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil: Emergence and expansion of the MDR serotype 6C-CC386 lineage
    Felipe P G Neves, Nayara T Cardoso, Aline R V Souza, Robert E Snyder, Mariel M Marlow, Tatiana C A Pinto, Lúcia M Teixeira, and Lee W Riley

    Oxford University Press (OUP)
    Objectives To determine the population structure and change in drug resistance of pneumococci colonizing children before and after the introduction of the 10-valent and 13-valent pneumococcal conjugate vaccines (PCV10/13) in Brazil. Methods We used MLST to analyse 256 pneumococcal isolates obtained from children aged <6 years before (2009-10; n = 125) and after (2014; n = 131) the introduction of the PCV10 and PCV13. Antimicrobial susceptibility and capsular types were previously determined. Results We identified 97 different STs. Ninety (35.2%) isolates were related to international clones. The most frequent lineages were serogroup 6-CC724 (where CC stands for clonal complex) and the MDR serotype 6C-CC386 in the pre- and post-PCV10/13 periods, respectively. Penicillin-non-susceptible pneumococci (PNSP) formed 24% and 38.9% of the pre- and post-PCV10/13 isolates, respectively (P = 0.01). In the pre-PCV10/13 period, serotype 14-ST156 was the predominant penicillin-non-susceptible lineage, but it was not detected in the post-PCV10/13 period. Serotype 14-ST156 and serotype 19A-ST320 complex isolates had the highest penicillin and ceftriaxone MICs in the pre- and post-PCV10/13 periods, respectively. In turn, serotype 6C-CC386 comprised almost 30% of the PNSP and over 40% of the erythromycin-resistant isolates (MIC >256 mg/L) in the post-PCV10/13 period. Conclusions Although PNSP strains were polyclonal, most resistant isolates belonged to a single genotype from each period. Higher erythromycin resistance prevalence (42%) in the post-PCV10/13 period was mainly attributed to MDR serotype 6C-CC386. Ongoing surveillance of pneumococcal clonal composition is important to evaluate PCV use outcomes and to identify factors other than PCVs that drive pneumococcal drug resistance evolution.

  • Characterization of a new genotype of Betapapillomavirus HPV 17 through L1, E7, E7 and LCR sequences
    L. OLIVEIRA, L. SANTOS, and F. NEVES

    AEPress, s.r.o.
    Human papillomavirus (HPV) exhibits epithelial and mucosal tropism. HPV type 17 belongs to the Betapapillomavirus genus and molecular cloning experiments have identified two subtypes (17a and 17b) isolated from epidermodysplasia verruciformis (EV). HPV subtypes are characterized by dissimilarities from 2 to 10% at the nucleotide level from their referenced HPV. The aim of this study was to characterize the L1, E6, E7 and LCR sequences from an isolate, which was recovered from the oral mucosa of an asymptomatic 63 year-old woman. The whole late gene 1 (L1) was amplified using several sets of primers. The complete early genes 6 and 7 (E6, E7) and the long control region (LCR) were amplified using specific primers. Potential binding sites for transcriptional factors within the LCR were also investigated. Within these sets, the DNA sequence was altered at 91 positions (68 in L1, 13 in E6, 8 in E7, and 2 in LCR sequences). L1 analysis showed high dissimilarity compared with the HPV 17 prototype, reaching 4% of nucleotide substitutions and leading to a probable third 17 subtype. The E6 oncoprotein presented the highest modification among the sequences studied, with four amino acid changes in comparison with the prototype isolate. One amino acid was modified at a position 62 (S-T), a zinc-binding domain (CxxC(C)29 CxxC). Our findings provide data on genetic variations seen in this genotype, reaching to dichotomic branching and pointing to an evolutionary process.

  • Major globally disseminated clonal complexes of antimicrobial resistant enterococci associated with infections in cancer patients in Brazil
    Barbara A. Santos, Jéssica S. Oliveira, Nayara T. Cardoso, André V. Barbosa, Silvana V. Superti, Lúcia M. Teixeira, and Felipe P.G. Neves

    Elsevier BV
    Cancer and hematological malignancies constitute major comorbidities in enterococcal infections, but little is known about the characteristics of enterococci affecting cancer patients. The aim of this study was to characterize 132 enterococcal clinical isolates obtained from cancer patients attending a Cancer Reference Center in Brazil between April 2013 and March 2014. Susceptibility to 17 antimicrobial agents was assessed by disk diffusion method. Resistance and virulence genes were investigated by PCR. Multilocus sequence typing (MLST) was performed for selected Enterococcus faecalis and Enterococcus faecium isolates. The predominant species was E. faecalis (108 isolates), followed by E. faecium (18), Enterococcus gallinarum (3), Enterococcus avium (2) and Enterococcus durans (1). Multidrug-resistant (MDR) isolates made up 44.7%, but all isolates were susceptible to fosfomycin, linezolid and glycopeptides. The most prevalent genes associated with erythromycin- and tetracycline-non susceptible isolates were erm(B) (47/71; 66.2%) and tet(M) (24/68; 35.3%), respectively. High-level resistance (HLR) to gentamicin was found in 22 (16.7%) isolates and 13 (59.1%) of them carried the aac(6')-Ie-aph(2″)-Ia gene. HLR to streptomycin was detected in 34 (25.8%) isolates, of which 15 (44.1%) isolates had the ant(6')-Ia gene. The most common virulence genes were gelE (48.9%), esp (30.5%) and asa1 (29.8%). MLST performed for 26 E. faecalis isolates revealed 18 different sequence-types (STs), with seven corresponding to novel STs (625, 626, 627, 628, 629, 630, and 635). On the other hand, nine of 10 E. faecium isolates analyzed by MLST belonged to a single clonal complex, comprised of mostly ST412, which emerged worldwide after mid-2000s, but also two novel STs (963 and 964). We detected major globally disseminated E. faecalis and E. faecium clonal complexes along with novel closely related STs, indicating the fitness and continuous evolution of these hospital-adapted lineages.

  • Pneumococcal nasopharyngeal carriage among children in Brazil prior to the introduction of the 10-valent conjugate vaccine: A culture- and PCR-based survey
    H. G. RODRIGUES, T. C. A. PINTO, R. R. BARROS, L. M. TEIXEIRA, and F. P. G. NEVES

    Cambridge University Press (CUP)
    SUMMARYWe performed two different approaches (broth enrichment step prior to culture (BEC) and PCR (BEPCR)) for detecting Streptococcus pneumoniae from nasopharyngeal specimens collected from 242 children aged &lt;6 years attending one hospital (n = 140) and one childcare centre (n = 102) in a major urban area in Brazil. These specimens were collected immediately before the introduction of the 10-valent pneumococcal conjugate vaccine (PCV10) and the 13-valent vaccine (PCV13) for routine use in Brazil. Results were compared with previous findings obtained with direct culture (DC) on a selective medium. Colonisation prevalence was 58·3% (n = 141), being higher among children attending the childcare centre (62·7% vs. 55%). The culture-based methods (DC and BEC) enabled the detection of S. pneumoniae in 119 (49·2%) and 115 (47·5%) children, respectively. The PCR-based method (BEPCR) was more sensitive and 137 (56·6%) carriers were identified. Twenty-six serogroups/serotypes were identified, predominantly 6B, 19F, 14, 6A, 15C and 23F. Multiple colonisation was observed in 13 (5·4%) children. The estimated serotypes coverage of available PCVs was 40·4% for the 10-valent (included in the Brazilian immunisation programme) and 55·8% for the 13-valent (only available in private clinics). The use of robust approaches to obtain a more realistic insight about the asymptomatic carrier status is of paramount importance to estimate and assess the impact of vaccine implementation. The combination between culture-based and molecular methods constitutes a suitable strategy.

  • Pneumococcal carriage among children after four years of routine 10-valent pneumococcal conjugate vaccine use in Brazil: The emergence of multidrug resistant serotype 6C
    Felipe P.G. Neves, Nayara T. Cardoso, Robert E. Snyder, Mariel A. Marlow, Claudete A.A. Cardoso, Lúcia M. Teixeira, and Lee W. Riley

    Elsevier BV
    BACKGROUND In 2010, the 10-valent pneumococcal conjugate vaccine (PCV10) was introduced free of charge in Brazil as part of the public immunization program. Here we investigated the carriage prevalence, colonization risk factors, capsular types, and antimicrobial resistance among pneumococcal isolates obtained from children in Brazil four years after routine PCV10 use. METHODS Between September and December 2014, we conducted a cross-sectional study among children<6years old who attended one public and two private clinics in Niterói, RJ, Brazil to evaluate pneumococcal nasopharyngeal carriage. Antimicrobial susceptibility and capsular types were determined for all isolates. RESULTS Of 522 children, 118 (22.6%) were pneumococcal carriers. Being≥2years old, attending childcare center, presenting with any symptoms, having acute or chronic respiratory disease, and residing in a slum were associated with pneumococcal carriage. The most prevalent capsular types were 6C (14.5%), 15B/C (11.5%), 11A/D (9.2%), and 6A (7.6%). PCV10 serotypes represented 2.5%. All isolates were susceptible to levofloxacin, rifampicin, and vancomycin. Penicillin non-susceptible pneumococci (PNSP) comprised 39%, with penicillin and ceftriaxone MICs ranging from 0.12-8.0μg/ml and 0.012-1.0μg/ml, respectively. The 33 (28%) erythromycin-resistant isolates (MICs of 1.5 to >256μg/ml) displayed the cMLSB (72.7%) or M (27.3%) phenotypes, harboring the erm(B) and/or mef(A/E) genes. High non-susceptibility rates (>20%) to clindamycin, erythromycin, penicillin, and tetracycline were largely explained by the prevalence of multidrug resistant (MDR) serotype 6C isolates. CONCLUSIONS Effects of universal childhood PCV10 use on carriage were evident, with the near elimination of PCV10 serotypes. The emergence of MDR serotype 6C isolates, however, is a concern. Ongoing surveillance to monitor serotype 6C increase in invasive diseases is warranted.

  • Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates
    Tatiana C. A. Pinto, Natalia S. Costa, Luciana F. S. Castro, Rachel L. Ribeiro, Ana Caroline N. Botelho, Felipe P. G. Neves, Jose Mauro Peralta, and Lucia M. Teixeira

    Springer Science and Business Media LLC
    Streptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques. Such methods, however, can be expensive, laborious or unable to accurately discriminate among certain serotypes. Therefore, determination of capsular types, although extremely important for epidemiological purposes and for estimating the impact of pneumococcal conjugate vaccines, is mainly restricted to research laboratories, being rarely performed in the clinical setting. In the present study, MALDI-TOF MS was evaluated as an alternative tool to characterize 416 pneumococcal isolates belonging to serotypes 6A, 6B, 6C, 9N, 9V or 14. For MALDI-TOF MS analysis, each isolate was submitted to an extraction protocol using formic acid and acetonitrile. Measurements were performed with a Bruker Microflex LT mass spectrometer using default parameters and generating spectra in the range of 2,000–20,000 m/z. Spectra were analyzed with the BioNumerics software v7.6. Isolates were mainly distributed according to the capsular type in a Neighbor Joining tree and serotypes investigated were successfully discriminated by the presence/absence of 14 selected biomarkers. The results suggest that MALDI-TOF MS is a promising alternative for typing pneumococcal strains, highlighting its usefulness for rapid and cost-effective routine application in clinical laboratories.

  • Zika: A scourge in urban slums
    Robert E. Snyder, Claire E. Boone, Claudete A. Araújo Cardoso, Fabio Aguiar-Alves, Felipe P. G. Neves, and Lee W. Riley

    Public Library of Science (PLoS)
    1 Division of Epidemiology, School of Public Health, University of California Berkeley, Berkeley, California, United States of America, 2 Maternal and Child Department, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil, 3 Pathology Program, Laboratório Universitário Rodolpho Albino, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil, 4 Department of Microbiology and Parasitology, Biomedical Institute, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil, 5 Division of Infectious Diseases and Vaccinology, School of Public Health, University of California Berkeley, Berkeley, California, United States of America

RECENT SCHOLAR PUBLICATIONS

  • Detection of Mycoplasma spp. in free-living seabirds
    SG de Brito, TS Dias, L dos Santos Machado, JB de Souza, NC da Cunha, ...
    Brazilian Journal of Microbiology, 1-6 2024

  • Antimicrobial Resistance in Streptococcus pneumoniae before and after the Introduction of Pneumococcal Conjugate Vaccines in Brazil: A Systematic Review
    PA Knupp-Pereira, AS Cabral, M Dolores, AB da Silva, HCC Pvoa, ...
    Antibiotics 13 (1), 66 2024

  • First Report of a Wastewater Treatment-Adapted Enterococcus faecalis ST21 Harboring vanA Gene in Brazil
    BO Farias, KS Montenegro, APA Nascimento, M Magaldi, ...
    Current Microbiology 80 (9), 313 2023

  • Prevalence, capsular types, antimicrobial resistance and risk factors associated with pneumococcal carriage among children after long-term 10-valent pneumococcal conjugate
    LBDP Fortuna, FM Miranda, IMF Antunes, AB Silva, AS Cabral, ...
    Vaccine 41 (19), 3111-3118 2023

  • Drug resistance in maternal and paediatric bacterial and fungal infections: Is COVID-19 changing the landscape
    FPG Neves, T Mestrovic, TCA Pinto
    Frontiers in microbiology 14, 1177669 2023

  • Carriage prevalence, serotype distribution, antimicrobial resistance, pspA typing and pilus islets of Streptococcus pneumoniae isolated from adults living in a Brazilian urban slum
    AB da Silva, NT Cardoso-Marques, de Moraes Dolores, LM Teixeira, ...
    Vaccine 41 (8), 1431-1437 2023

  • Biofilm formation, interaction and survival within A549 pneumocytes of Klebsiella pneumoniae clinical strains: identification of pulsotypes, multidrug-resistance and genes
    BA Nogueira, JGB Olivella, BR Sued-Karam, PMAP Ribeiro, FPG Neves, ...
    Brazilian Journal of Development, 55259-55287 2022

  • Streptococcus pneumoniae
    FPG Neves, TCA Pinto
    Molecular Typing in Bacterial Infections, Volume I, 139-152 2022

  • Virulence of Lactobacillus spp. misidentified as Enterococcus faecalis from children’s carious dentine
    GC Chianca, LAA Antunes, PO Ornellas, FPG Neves, HCC Pvoa, ...
    Acta Odontologica Scandinavica 80 (1), 21-28 2022

  • Carriage prevalence, serotype distribution, and antimicrobial susceptibility among pneumococcal isolates recovered from adults with systemic lupus erythematosus
    RPV Rezende, NT Cardoso-Marques, LAS Rodrigues, JPCL Almeida, ...
    Lupus 30 (11), 1863-1865 2021

  • Biofilm formation on breast implant surfaces by major gram-positive bacterial pathogens
    G Rezende-Pereira, JP Albuquerque, MC Souza, BA Nogueira, MG Silva, ...
    Aesthetic Surgery Journal 41 (10), 1144-1151 2021

  • Streptococcus pneumoniae prevalence in nasopharynx, oropharynx and gingival sulcus in Brazilian adults: A preliminary study
    PG Lomardo, NT Cardoso-Marques, LA Motta, TRS Aguiar, FPG Neves, ...
    Microbial Pathogenesis 156, 104924 2021

  • Going online in pandemic time: a DivulgaMicro workshop experience
    LM Andrade de Oliveira, E Cordeiro-Spinetti, FPG Neves, PS Sujii, ...
    Journal of microbiology & biology education 22 (1), 10.1128/jmbe. v22i1. 2493 2021

  • Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
    ARV Souza, SECM de Pina, NS Costa, FPG Neves, VLC Merquior, ...
    Scientific Reports 11 (1), 7936 2021

  • CRISPR elements and their association with antimicrobial resistance and virulence genes among vancomycin-resistant and vancomycin-susceptible enterococci recovered from human
    BA Dos Santos, JS de Oliveira, BM Parmanhani-da-Silva, RL Ribeiro, ...
    Infection, Genetics and Evolution 80, 104183 2020

  • Prevalence of PspA families and pilus islets among Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate
    PA Knupp-Pereira, NTC Marques, LM Teixeira, HCC Pvoa, FPG Neves
    Brazilian Journal of Microbiology 51, 419-425 2020

  • Differences in gram-positive bacterial colonization and antimicrobial resistance among children in a high income inequality setting
    FPG Neves, MA Marlow, G Rezende-Pereira, MG Pinheiro, ...
    BMC infectious diseases 19, 1-9 2019

  • Direct effect of the 13-valent pneumococcal conjugate vaccine use on pneumococcal colonization among children in Brazil
    FPG Neves, NT Cardoso, CAA Cardoso, LM Teixeira, LW Riley
    Vaccine 37 (36), 5265-5269 2019

  • Evolution of Penicillin Non-susceptibility Among Streptococcus pneumoniae Isolates Recovered From Asymptomatic Carriage and Invasive Disease Over 25 years
    TCA Pinto, FPG Neves, ARV Souza, LMA Oliveira, NS Costa, LFS Castro, ...
    Frontiers in microbiology 10, 486 2019

  • Population structure of Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil: emergence
    FPG Neves, NT Cardoso, ARV Souza, RE Snyder, MM Marlow, TCA Pinto, ...
    Journal of Antimicrobial Chemotherapy 73 (5), 1206-1212 2018

MOST CITED SCHOLAR PUBLICATIONS

  • Nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among children from Brazil before the introduction of the
    FPG Neves, TCA Pinto, MA Corra, R dos Anjos Barreto, ...
    BMC infectious diseases 13, 1-7 2013
    Citations: 63

  • Antimicrobial resistance profiles and genetic characterisation of macrolide resistant isolates of Streptococcus agalactiae
    PAM Nakamura, RBB Schuab, FPG Neves, CFA Pereira, GR de Paula, ...
    Memrias do Instituto Oswaldo Cruz 106, 119-122 2011
    Citations: 52

  • Multidrug-resistant nontuberculous mycobacteria isolated from cystic fibrosis patients
    PHC Cndido, LS Nunes, EA Marques, TW Folescu, FS Coelho, ...
    Journal of clinical microbiology 52 (8), 2990-2997 2014
    Citations: 51

  • Pneumococcal carriage among children after four years of routine 10-valent pneumococcal conjugate vaccine use in Brazil: the emergence of multidrug resistant serotype 6C
    FPG Neves, NT Cardoso, RE Snyder, MA Marlow, CAA Cardoso, ...
    Vaccine 35 (21), 2794-2800 2017
    Citations: 45

  • Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
    AA Uehara, ELT Amorin, MF Ferreira, CF Andrade, MBM Clementino, ...
    Journal of clinical microbiology 49 (12), 4208-4212 2011
    Citations: 41

  • Zika: A scourge in urban slums
    RE Snyder, CE Boone, CAA Cardoso, F Aguiar-Alves, FPG Neves, ...
    PLoS Neglected Tropical Diseases 11 (3), e0005287 2017
    Citations: 39

  • Characteristics related to antimicrobial resistance and biofilm formation of widespread methicillin-resistant Staphylococcus epidermidis ST2 and ST23 lineages in Rio de Janeiro
    NLP Iorio, RF Caboclo, MB Azevedo, AG Barcellos, FPG Neves, ...
    Diagnostic microbiology and infectious disease 72 (1), 32-40 2012
    Citations: 37

  • Antimicrobial susceptibility patterns, emm type distribution and genetic diversity of Streptococcus pyogenes recovered in Brazil
    GP Aras, RBB Schuab, FPG Neves, RR Barros
    Memrias do Instituto Oswaldo Cruz 109, 935-939 2014
    Citations: 33

  • Phenotypic and molecular characterization of optochin-resistant Streptococcus pneumoniae isolates from Brazil, with description of five novel mutations in the atpC gene
    TCA Pinto, ARV Souza, SECM de Pina, NS Costa, AA Borges Neto, ...
    Journal of clinical microbiology 51 (10), 3242-3249 2013
    Citations: 33

  • Panton-Valentine leukocidin (PVL) gene carriage among Staphylococcus aureus strains with SCCmec types I, III, IV, and V recovered from cystic fibrosis pediatric patients in Brazil
    DF Lima, NBV Brazo, TW Folescu, FP Neves, AG Ferreira, EA Santos, ...
    Diagnostic microbiology and infectious disease 78 (1), 59-62 2014
    Citations: 28

  • Population structure of Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil: emergence
    FPG Neves, NT Cardoso, ARV Souza, RE Snyder, MM Marlow, TCA Pinto, ...
    Journal of Antimicrobial Chemotherapy 73 (5), 1206-1212 2018
    Citations: 26

  • Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates
    TC Pinto, NS Costa, LF Castro, R Rachel L., AC Botelho, FP Neves, ...
    Scientific Reports 7, 45572 2017
    Citations: 26

  • Antimicrobial susceptibility and genetic diversity of Streptococcus agalactiae recovered from newborns and pregnant women in Brazil
    VC Souza, FCO Kegele, SR Souza, FPG Neves, GR de Paula, RR Barros
    Scandinavian Journal of Infectious Diseases 45 (10), 780-785 2013
    Citations: 26

  • Evolution of Penicillin Non-susceptibility Among Streptococcus pneumoniae Isolates Recovered From Asymptomatic Carriage and Invasive Disease Over 25 years
    TCA Pinto, FPG Neves, ARV Souza, LMA Oliveira, NS Costa, LFS Castro, ...
    Frontiers in microbiology 10, 486 2019
    Citations: 25

  • Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil
    RF Ferreira, AMF Cerqueira, TX Castro, EO Ferreira, FPG Neves, ...
    Revista Brasileira de Parasitologia Veterinria 23, 301-308 2014
    Citations: 24

  • Bacteraemia associated with a vancomycin-resistant Enterococcus gallinarum strain harbouring both the vanA and vanC1 genes
    VLC Merquior, FP Goncalves Neves, RL Ribeiro, RS Duarte, ...
    Journal of medical microbiology 57 (2), 244-245 2008
    Citations: 24

  • Emergence of the vanA genotype among Enterococcus gallinarum isolates colonising the intestinal tract of patients in a university hospital in Rio de Janeiro, Brazil
    FPG Neves, RL Ribeiro, RS Duarte, LM Teixeira, VLC Merquior
    International journal of antimicrobial agents 33 (3), 211-215 2009
    Citations: 23

  • Differences in gram-positive bacterial colonization and antimicrobial resistance among children in a high income inequality setting
    FPG Neves, MA Marlow, G Rezende-Pereira, MG Pinheiro, ...
    BMC infectious diseases 19, 1-9 2019
    Citations: 18

  • Serotypes, antimicrobial resistance and genotypes of Streptococcus pneumoniae associated with infections in cancer patients in Brazil.
    NFP Cardoso NT, Santos BA, Barbosa AV, Superti SV, Teixeira LM
    Diagn Microbiol Infect Dis 2016
    Citations: 16

  • CRISPR elements and their association with antimicrobial resistance and virulence genes among vancomycin-resistant and vancomycin-susceptible enterococci recovered from human
    BA Dos Santos, JS de Oliveira, BM Parmanhani-da-Silva, RL Ribeiro, ...
    Infection, Genetics and Evolution 80, 104183 2020
    Citations: 14