Garima Singh
@mzu.edu.in
Assistant Professor
Mizoram University
RESEARCH, TEACHING, or OTHER INTERESTS
Plant Science, Agricultural and Biological Sciences, Plant Science, Plant Science
Scopus Publications
Scopus Publications
B.C. Lalremruata, P.C. Vanlalhluna, and Garima Singh
Elsevier BV
Jyoti Mehta, Gomaa Ali, G. P. Singh, Randa Althobiti, and Kuldeep Bauddh
Egyptian Knowledge Bank
Garima Singh, Ajit Kumar Passari, N. Senthil Kumar, Brijesh Kumar, S. Chandra Nayak, Heera Ram, and Bhim Pratap Singh
Springer Science and Business Media LLC
Jyoti Mehta, Nishant Kumar, Rakesh Kr Singh, Moharana Choudhury, G P Singh, and Kuldeep Bauddh
Medic Scientific
Cadmium Sulfide nanoparticles (CdS NPs) were synthesised and their characteristics were evaluated using XRD (X-ray diffraction), FTIR (Fourier transform infrared), FESEM (Field emission scanning electron microscopy), EDAX (Energy Dispersive X-ray Analysis), and UV-Vis (Ultraviolet-visible). Further, the impacts of CdS NPs on the growth, pigment content and antioxidative defence system of Pistia stratiotes were studied. The exposure of CdS NPs to Pistia stratiotes caused the negative repercussions, which were tested using a variety of biological tests. The findings demonstrated that CdS NPs were hazardous to the Pistia plant at high concentrations, while they were easy to grow at lower concentrations up to 30 ml/l. Both growth and colour characteristics declined at 40 mg/l concentration of CdS NPs. Antioxidant enzymes' activity like superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) also decreased at 40 ml/l on 60 days. The Protein and photosynthetic pigments were declined on 60 days at 40ml/l concentration however, the level of both the parameters was found increased in comparison with control. The results demonstrated that the higher concentrations of CdS NPs are toxic to Pistia, resulting in decreased growth and significant alterations in antioxidant enzyme activity. These findings suggest that Pistia can be used as a bio-indicator for CdS NPs contaminated water.
Abeer Hashem, Garima Singh, Bhim Pratap Singh, Khalid F. Almutairi, Naveen Kumar Kalagatur, and Elsayed Fathi Abd_Allah
Elsevier
Pramod Kumar, Heera Ram, Chandra Kala, Priya Kashyap, Garima Singh, Charu Agnihotri, Bhim Pratap Singh, Ashok Kumar, and Anil Panwar
Informa UK Limited
Abstract The DPP-4 inhibition is an interesting target for the development of antidiabetic agents which promotes the longevity of GPL-1(Glucagon-like peptide 1). The current study was intended to assess DPP-4(Dipeptidyl Peptidase-4) inhibition mediated antidiabetic effect of phytocompounds of an aqueous fruit extract of Withania coagulans (Stocks) Dunal by in-vitro, in-silico and in-vivo approaches. The phytoconstituents screening was executed by LCMS (Liquid Chromatography with tandem mass spectrometry). The in-vitro and in-vivo, DPP-4 assays were performed by using available kits. The in-vitro DPP-4 activity was inhibited up to 68.3% by the test extract. Accordingly, in-silico determinations of molecular docking, molecular dynamics and pharmacokinetics were performed between the target enzyme DPP-4 and leading phytocompounds. The molecular dynamics authenticated the molecular docking data by crucial parameters of cytosolic milieu by the potential energy, RSMD (Root Mean Square Deviation), RSMF (Root Mean Square Fluctuation), system density, NVT (Number of particles at fixed volume, ensemble) and NPT (Number of particles at fixed pressure, ensemble). Accordingly, ADMET predictions assessed the druggability profile. Subsequently, the course of the test extract and the sitagliptin (positive control), instigated significant (p ≤ 0.001) ameliorations in HOMA indices and the equal of antioxidants in nicotinamide-streptozotocin induced type 2 diabetic animal model. Compassionately, the histopathology represented increased pancreatic cellular mass which caused in restoration of histoarchitectures. It has been concluded that phytoconstituents in W. coagulans aqueous fruit extract can regulate DPP-4, resulting in improved glucose homeostasis and enhanced endocrinal pancreatic cellular mass. Communicated by Ramaswamy H. Sarma
Noopur Jaipal, Heera Ram, Jaykaran Charan, Anshuman Dixit, Garima Singh, Bhim P. Singh, Ashok Kumar, and Anil Panwar
Wiley
AbstractHydrophilic bioactive compounds are copiously exhibited in aqueous extracts owed to solubility. The study was assigned to assess the ability of phytoconstituents of aqueous pod extract of Prosopis cineraria to inhibit 3‐hydroxy‐3‐methylglutary‐coenzyme A (HMG‐CoA) reductase activity and regression in atherosclerotic plaque through in vitro, in vivo, and in silico assessments along with phytochemistry of extract. The test extract exhibited 17 leading compounds as examined by Liquid Chromatograph Triple Quadrupole Mass Spectroscope. In vitro assay of test extract showed 78.1% inhibition of HMG‐CoA inhibition (IC50 was 0.03 μg/ml). In vivo assessments, hypercholesterolemia was induced by supplementing cholesterol powder and a high‐fat diet. The treatment of test extract caused significant (p ≤ 0.001) improvements in the lipid profile and antioxidant levels. Subsequently, the reductions in the atherosclerotic plaque and improved lumen volume were pointedly observed. In silico analyses of molecular docking revealed potent interaction capabilities of cloprostenol with the target protein of HMGR. The interactions were validated through structural simulations of the molecular dynamics such as root mean square fluctuation, the radius of gyration, and solvent accessible surface area. The druggability of potent compounds was also examined. The results revealed that phytoconstituents of the test extract could inhibit HMGR and regress atherosclerotic plaque.
Jaykaran Charan, Priyanka Riyad, Heera Ram, Ashok Purohit, Sneha Ambwani, Priya Kashyap, Garima Singh, Abeer Hashem, Elsayed Fathi Abd_Allah, Vijai Kumar Gupta,et al.
Public Library of Science (PLoS)
[This corrects the article DOI: 10.1371/journal.pone.0264646.].
Jaykaran Charan, Priyanka Riyad, Heera Ram, Ashok Purohit, Sneha Ambwani, Priya Kashyap, Garima Singh, Abeer Hashem, Elsayed Fathi Abd_Allah, Vijai Kumar Gupta,et al.
Public Library of Science (PLoS)
The assigned work was aimed to examine the capability of phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd to inhibit HMG-CoA reductase and regression of the atherosclerotic plaque. The chemical fingerprinting of the test extract was assessed by LC-MS/MS. Consequently, the analyses of in-vitro, in-vivo, and in-silico were executed by using the standard protocols. The in-vitro assessment of the test extract revealed 74.1% inhibition of HMG-CoA reductase. In-vivo assessments of the test extract indicated that treated hypercholesterolemic rabbits exhibited a significant (P≤0.001) amelioration in the biomarker indices of the dyslipidaemia i.e., atherogenic index, Castelli risk index(I&II), atherogenic coefficient along with lipid profile. Subsequently, significant reductions were observed in the atherosclerotic plaque and antioxidant levels. The in-silico study of molecular docking shown interactions capabilities of the leading phytoconstituents of the test extract i.e., eicosanoic acid, linoleic acid, and flavan-3-ol with target protein of HMG-CoA reductase. The values of RSMF and potential energy of top docked complexes were show significant interactions. Accordingly, the free energy of solvation, interaction angle, radius of gyration and SASA were shown significant stabilities of top docked complex. The cumulative data of results indicate phytoconstituents of an aqueous seed extract of Acacia senegal have capabilities to inhibit the HMG-CoA reductase and improve the levels of antioxidants.
Garima Singh, Ruth Zomuansangi, Vanlalpeki Hnamte, Akriti Tirkey, Bhim Pratap Singh, Prashant Kumar Singh, Zothanpuia, Vijai Kumar Gupta, Purbajyoti Deka, Kalidas Upadhyaya,et al.
Elsevier
Mukesh Kumar Yadav, Poonam Patel, Ajay Kumar, Arpan Modi, Punuri Jayasekhar Babu, Zothanpuia, Liansangmawii Chhakchhuak, Kaushalendra, Garima Singh, Amit Kumar Mishra,et al.
Springer International Publishing
Ruth Zomuansangi, Bhim Pratap Singh, Garima Singh, Zothanpuia, Prashant Kumar Singh, Jae Jun Song, Arun S. Kharat, Purbhajyoti Deka, and Mukesh Kumar Yadav
Elsevier
Ruth Zomuansangi, Zothanpuia, Prashant Kumar Singh, Bhim Pratap Singh, Garima Singh, Purbajyoti Deka, Jae Jun Song, and Mukesh Kumar Yadav
Elsevier
Rahul Prasad Singh, Sandeep Kumar Singh, Ajay Kumar, Arpan Modi, Mukesh Kumar Yadav, Punuri Jayasekhar Babu, Garima Singh, Kaushalendra, Naveen Chandra Joshi, Avinash Chandra Rai,et al.
Elsevier
Sandeep Kumar Singh, Ajay Kumar, Mukesh Kumar Yadav, Garima Singh, Punuri Jayasekhar Babu, Zothanpuia, Liansangmawii Chhakchhuak, Naveen Chandra Joshi, Avinash Chandra Rai, Avinash Singh,et al.
Elsevier
Heera Ram, Pramod Kumar, Ashok Purohit, Priya Kashyap, Suresh Kumar, Shivani Kumar, Garima Singh, Abdulaziz A. Alqarawi, Abeer Hashem, Elsayed Fathi Abd-Allah,et al.
Springer Science and Business Media LLC
Abstract Context Withania coagulans (Stocks) Dunal fruits are used in the therapeutics of several ailments due to possessing of potent phytoconstituents which is also used traditionally for curing the diabetes. Objective The present study was assessing the amelioration potential of the phytochemicals of an ethanol fruit extract of W. coagulans (Stocks) Dunal in the HOMA (Homeostatic model assessment) indices and pancreatic endocrinal tissues by inhibition of DPP-4 and antioxidants activities. Material and methods The identification of phytoconstituents of the test extract was performed by LCMS. Further, assessments of in-vitro, in-vivo and in-silico were achieved by following standard methods. In-vivo studies were conducted on type-2 diabetic rats. Results The chosen extract inhibited DPP-4 activity by 63.2% in an in vitro assay as well as significantly inhibit serum DPP-4 levels. Accordingly, the administration of the ethanol fruit extract resulted in a significant (P ≤ 0.001) alterations in the lipid profile, antioxidant levels, and HOMA indices. Moreover, pancreatic endocrinal tissues (islet of Langerhans) appeared to have the restoration of normal histoarchitecture as evidenced by increased cellular mass. Molecular docking (Protein-ligands) of identified phytoconstituents with DPP-4 (target enzyme) shown incredibly low binding energy (Kcal/mol) as required for ideal interactions. ADMET analysis of the pharmacokinetics of the identified phytoconstituents indicated an ideal profile as per Lipinski laws. Conclusion It can be concluded that the phytoconstituents of an ethanol fruit extract of W. coagulans have the potential to inhibit DPP-4 which result in improved glucose homeostasis and restoration of pancreatic endocrinal tissues in type-2 diabetic rats.
M.A. Iquebal, Ajit Kumar Passari, Jaisri Jagannadham, Farzana Ahmad, Vincent Vineeth Leo, Garima Singh, Sarika Jaiswal, Anil Rai, Dinesh Kumar, and Bhim Pratap Singh
Elsevier BV
Abeer Hashem, Adnan Akhter, Abdulaziz A. Alqarawi, Garima Singh, Khalid F. Almutairi, and Elsayed Fathi Abd_Allah
Elsevier BV
Purbajyoti Deka, Gajanan T. Mehetre, Esther Lalnunmawii, Kalidas Upadhyaya, Garima Singh, Abeer Hashem, Al-Bandari Fahad Al-Arjani, Elsayed Fathi Abd_Allah, and Bhim Pratap Singh
MDPI AG
Traditional fermented foods have been recognized by various communities to be good for health since ancient times. There is a provincial legacy of traditional fermented foods among the ethnic population of North-East India. Fermented bamboo shoots (local name: Tuaither), soybeans (Bekang), and pork fat (Sa-um) are famous in the Mizoram state and represent a primary portion of the daily diet. These foods are prepared using methods based on cultural traditions inherited from previous generations, and prepared using a relatively uncontrolled fermentation process. Analysis of the bacterial diversity in these foods can provide important information regarding the flavor and texture of the final products of fermentation. Unfortunately, studies on the microbial composition and health benefits of such traditional fermented foods have rarely been documented. Therefore, the present study aims to highlight this bacterial diversity, along with the proximate composition of different traditional fermented foods (Tuaither, Bekang and Sa-um) primarily consumed in Mizoram state, India. Samples were collected on three different days of fermentation (3rd, 5th and 7th day), and bacterial diversity analysis was performed using the V3-V4 variable region of 16S rRNA gene with Illumina sequencing. Results revealed differences in the bacterial composition of dominant group members among all of the three food types. Firmicutes (82.72–94.00%), followed by Proteobacteria (4.67–15.01%), were found to dominate to varying degrees in all three of the fermented foods. However, at genus level high variation was observed in bacterial composition among these three different types of fermented foods. Lactobacillus (91.64–77.16%), Staphylococcus (52.00–17.90%), and Clostridium (72.48–55.40%) exhibited the highest relative abundances in the Tuaither, Bekang and Sa-um foods, respectively, in descending order from the 3rd to 7th day of fermentation. A few of the bacterial genera such as Lactobacilli were positively correlated with fermented bamboo shoot samples, and Staphylococcus was positively correlated with protein, carbohydrate and crude fiber content in soybean samples. In general, Tuaither, Bekang and Sa-um exhibited distinct differences in bacterial composition. This variation may be due to differences in the raw materials and/or methods used in the preparation of the different fermented food products. This is the first study to describe the bacterial composition of these traditional fermented foods using high-throughput sequencing techniques, and could help to drive research attention to comprehensive studies on improving understanding of the role of microbial communities in the preparation of traditional foods and their health benefits.
Gajanan T. Mehetre, Vincent Vineeth Leo, Garima Singh, Antonina Sorokan, Igor Maksimov, Mukesh Kumar Yadav, Kalidas Upadhyaya, Abeer Hashem, Asma N. Alsaleh, Turki M. Dawoud,et al.
MDPI AG
Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.
Ajit Kumar Passari, Vincent Vineeth Leo, Garima Singh, Loknath Samanta, Heera Ram, Chandra Nayak Siddaiah, Abeer Hashem, Al-Bandari Fahad Al-Arjani, Abdulaziz A. Alqarawi, Elsayed Fathi Abd_Allah,et al.
MDPI AG
Reactive oxygen species (ROS) and other free radicals cause oxidative damage in cells under biotic and abiotic stress. Endophytic microorganisms reside in the internal tissues of plants and contribute to the mitigation of such stresses by the production of antioxidant enzymes and compounds. We hypothesized that the endophytic actinobacterium Streptomyces sp. strain DBT34, which was previously demonstrated to have plant growth-promoting (PGP) and antimicrobial properties, may also have a role in protecting plants against several stresses through the production of antioxidants. The present study was designed to characterize catalase and superoxide dismutase (SOD), two enzymes involved in the detoxification of ROS, in methanolic extracts derived from six endophytic actinobacterial isolates obtained from the traditional medicinal plant Mirabilis jalapa. The results of a preliminary screen indicated that Streptomyces sp. strain DBT34 was the best overall strain and was therefore used in subsequent detailed analyses. A methanolic extract of DBT34 exhibited significant antioxidant potential in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays. The cytotoxicity of DBT34 against liver hepatocellular cells (HepG2) was also determined. Results indicated that methanolic extract of Streptomyces sp. strain DBT34 exhibited significant catalase and SOD-like activity with 158.21 U resulting in a 55.15% reduction in ROS. The IC50 values of a crude methanolic extract of strain DBT34 on DPPH radical scavenging and ABTS radical cation decolorization were 41.5 µg/mL and 47.8 µg/mL, respectively. Volatile compounds (VOC) were also detected in the methanolic extract of Streptomyces sp. strain DBT34 using GC-MS analysis to correlate their presence with bioactive potential. Treatments of rats with DBT34 extract and sitagliptin resulted in a significant (p ≤ 0.001) reduction in total cholesterol, LDL-cholesterol, and VLDL-cholesterol, relative to the vehicle control and a standard diabetic medicine. The pancreatic histoarchitecture of vehicle control rats exhibited a compact volume of isolated clusters of Langerhans cells surrounded by acinies with proper vaculation. An in-vivo study of Streptomyces sp. strain DBT34 on chickpea seedlings revealed an enhancement in its antioxidant potential as denoted by lower IC50 values for DPPH and ABTS radical scavenging activity under greenhouse conditions in relative comparison to control plants. Results of the study indicate that strain DBT34 provides a defense mechanism to its host through the production of antioxidant therapeutic agents that mitigate ROS in hosts subjected to biotic and abiotic stresses.
Ajit Kumar Passari, Kalidas Upadhyaya, Garima Singh, Ahmed M. Abdel-Azeem, Sugitha Thankappan, Sivakumar Uthandi, Abeer Hashem, Elsayed Fathi Abd_Allah, Jahangir Ahmed Malik, Alqarawi AS,et al.
Public Library of Science (PLoS)
Biotic stresses in plants have a significant impact on agricultural productivity. In the present study, in vivo experiments were conducted to determine the physiological responses of tomato (Solanum lycopersicum L.) seedlings by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus isolate BPSAC147 under greenhouse conditions. Further, photochemical quantum yield of photosystem II (PSII) (Fv/Fm), photochemical quenching (qP) and non-photochemical (NPQ) were calculated in seedlings inoculated with S. thermocarboxydus (T1) and were compared with control (T0) plants. Furthermore, the electron transport rate (ETR) of PSII exhibited a significant increase in T1 plants, relative to T0 plants. These results indicate that inoculation of tomato seedlings with S. thermocarboxydus had a positive effect on the process of photosynthesis, resulting in enhanced chlorophyll fluorescence parameters due to increased ETR in the thylakoid membrane. GC-MS analysis showed significant differences in the volatile compounds in the different treatments performed under greenhouse conditions. The present study suggests that S. thermocarboxydus can be used as new biocontrol agent to control Fusarium wilt in tomato crops and enhance productivity by enhancing photosynthesis.
Abeer Hashem, Ashwani Kumar, Abeer M. Al-Dbass, Abdulaziz A. Alqarawi, Al-Bandari Fahad Al-Arjani, Garima Singh, Muhammad Farooq, and Elsayed Fathi Abd_Allah
Elsevier BV