DPP-4 inhibition mediated antidiabetic potential of phytoconstituents of an aqueous fruit extract of Withania coagulans (Stocks) Dunal: in-silico, in-vitro and in-vivo assessments Pramod Kumar, Heera Ram, Chandra Kala, Priya Kashyap, Garima Singh, et al. Journal of Biomolecular Structure and Dynamics, 2023 The DPP-4 inhibition is an interesting target for the development of antidiabetic agents which promotes the longevity of GPL-1(Glucagon-like peptide 1). The current study was intended to assess DPP-4(Dipeptidyl Peptidase-4) inhibition mediated antidiabetic effect of phytocompounds of an aqueous fruit extract of Withania coagulans (Stocks) Dunal by in-vitro, in-silico and in-vivo approaches. The phytoconstituents screening was executed by LCMS (Liquid Chromatography with tandem mass spectrometry). The in-vitro and in-vivo, DPP-4 assays were performed by using available kits. The in-vitro DPP-4 activity was inhibited up to 68.3% by the test extract. Accordingly, in-silico determinations of molecular docking, molecular dynamics and pharmacokinetics were performed between the target enzyme DPP-4 and leading phytocompounds. The molecular dynamics authenticated the molecular docking data by crucial parameters of cytosolic milieu by the potential energy, RSMD (Root Mean Square Deviation), RSMF (Root Mean Square Fluctuation), system density, NVT (Number of particles at fixed volume, ensemble) and NPT (Number of particles at fixed pressure, ensemble). Accordingly, ADMET predictions assessed the druggability profile. Subsequently, the course of the test extract and the sitagliptin (positive control), instigated significant (p ≤ 0.001) ameliorations in HOMA indices and the equal of antioxidants in nicotinamide-streptozotocin induced type 2 diabetic animal model. Compassionately, the histopathology represented increased pancreatic cellular mass which caused in restoration of histoarchitectures. It has been concluded that phytoconstituents in W. coagulans aqueous fruit extract can regulate DPP-4, resulting in improved glucose homeostasis and enhanced endocrinal pancreatic cellular mass. Communicated by Ramaswamy H. Sarma
HMG-CoA reductase inhibition medicated hypocholesterolemic and antiatherosclerotic potential of phytoconstituents of an aqueous pod extract of Prosopis cineraria (L.) Druce: In silico, in vitro, and in vivo studies Noopur Jaipal, Heera Ram, Jaykaran Charan, Anshuman Dixit, Garima Singh, et al. Efood, 2022 Hydrophilic bioactive compounds are copiously exhibited in aqueous extracts owed to solubility. The study was assigned to assess the ability of phytoconstituents of aqueous pod extract of Prosopis cineraria to inhibit 3‐hydroxy‐3‐methylglutary‐coenzyme A (HMG‐CoA) reductase activity and regression in atherosclerotic plaque through in vitro, in vivo, and in silico assessments along with phytochemistry of extract. The test extract exhibited 17 leading compounds as examined by Liquid Chromatograph Triple Quadrupole Mass Spectroscope. In vitro assay of test extract showed 78.1% inhibition of HMG‐CoA inhibition (IC50 was 0.03 μg/ml). In vivo assessments, hypercholesterolemia was induced by supplementing cholesterol powder and a high‐fat diet. The treatment of test extract caused significant (p ≤ 0.001) improvements in the lipid profile and antioxidant levels. Subsequently, the reductions in the atherosclerotic plaque and improved lumen volume were pointedly observed. In silico analyses of molecular docking revealed potent interaction capabilities of cloprostenol with the target protein of HMGR. The interactions were validated through structural simulations of the molecular dynamics such as root mean square fluctuation, the radius of gyration, and solvent accessible surface area. The druggability of potent compounds was also examined. The results revealed that phytoconstituents of the test extract could inhibit HMGR and regress atherosclerotic plaque.
Ameliorations in dyslipidemia and atherosclerotic plaque by the inhibition of HMG-CoA reductase and antioxidant potential of phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd in rabbits Jaykaran Charan, Priyanka Riyad, Heera Ram, Ashok Purohit, Sneha Ambwani, et al. Plos One, 2022 The assigned work was aimed to examine the capability of phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd to inhibit HMG-CoA reductase and regression of the atherosclerotic plaque. The chemical fingerprinting of the test extract was assessed by LC-MS/MS. Consequently, the analyses of in-vitro, in-vivo, and in-silico were executed by using the standard protocols. The in-vitro assessment of the test extract revealed 74.1% inhibition of HMG-CoA reductase. In-vivo assessments of the test extract indicated that treated hypercholesterolemic rabbits exhibited a significant (P≤0.001) amelioration in the biomarker indices of the dyslipidaemia i.e., atherogenic index, Castelli risk index(I&II), atherogenic coefficient along with lipid profile. Subsequently, significant reductions were observed in the atherosclerotic plaque and antioxidant levels. The in-silico study of molecular docking shown interactions capabilities of the leading phytoconstituents of the test extract i.e., eicosanoic acid, linoleic acid, and flavan-3-ol with target protein of HMG-CoA reductase. The values of RSMF and potential energy of top docked complexes were show significant interactions. Accordingly, the free energy of solvation, interaction angle, radius of gyration and SASA were shown significant stabilities of top docked complex. The cumulative data of results indicate phytoconstituents of an aqueous seed extract of Acacia senegal have capabilities to inhibit the HMG-CoA reductase and improve the levels of antioxidants.
Distribution and antimicrobial potential of endophytic fungi associated with ethnomedicinal plant Melastoma malabathricum L. Journal of Environmental Biology, 2016
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HMG‐CoA reductase inhibition medicated hypocholesterolemic and antiatherosclerotic potential of phytoconstituents of an aqueous pod extract of Prosopis cineraria (L.) Druce: In … N Jaipal, H Ram, J Charan, A Dixit, G Singh, BP Singh, A Kumar, ... eFood 3 (6), e42 , 2022 2022 Citations: 12
DPP-4 inhibition mediated antidiabetic potential of phytoconstituents of an aqueous fruit extract of Withania coagulans (Stocks) Dunal: in-silico, in-vitro and in-vivo assessments P Kumar, H Ram, C Kala, P Kashyap, G Singh, C Agnihotri, BP Singh, ... Journal of Biomolecular Structure and Dynamics, 1-23 , 2022 2022 Citations: 17
Ameliorations in dyslipidemia and atherosclerotic plaque by the inhibition of HMG-CoA reductase and antioxidant potential of phytoconstituents of an aqueous seed extract of … J Charan, P Riyad, H Ram, A Purohit, S Ambwani, P Kashyap, G Singh, ... PloS one 17 (3), e0264646 , 2022 2022 Citations: 22
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Improvements in HOMA indices and pancreatic endocrinal tissues in type 2-diabetic rats by DPP-4 inhibition and antioxidant potential of an ethanol fruit extract of Withania … H Ram, P Kumar, A Purohit, P Kashyap, S Kumar, S Kumar, G Singh, ... Nutrition & metabolism 18 (1), 1-17 , 2021 2021 Citations: 31
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Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea A Hashem, A Kumar, AM Al-Dbass, AA Alqarawi, ABF Al-Arjani, G Singh, ... Saudi journal of biological sciences 26 (3), 614-624 , 2019 2019.0 Citations: 292
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Evaluation of phenolic content variability along with antioxidant, antimicrobial, and cytotoxic potential of selected traditional medicinal plants from India G Singh, AK Passsari, VV Leo, VK Mishra, S Subbarayan, BP Singh, ... Frontiers in plant science 7, 407 , 2016 2016.0 Citations: 173
Pharmacological potential of Bidens pilosa L. and determination of bioactive compounds using UHPLC-QqQ LIT-MS/MS and GC/MS G Singh, AK Passsari, P Singh, VV Leo, S Subbarayan, B Kumar, ... BMC complementary and alternative medicine 17 (1), 1-16 , 2017 2017.0 Citations: 100
Enhancement of disease resistance, growth potential, and photosynthesis in tomato (Solanum lycopersicum) by inoculation with an endophytic actinobacterium, Streptomyces … AK Passari, K Upadhyaya, G Singh, AM Abdel-Azeem, S Thankappan, ... PloS one 14 (7), e0219014 , 2019 2019.0 Citations: 85
Mycorrhizal fungi induced activation of tomato defense system mitigates Fusarium wilt stress A Hashem, A Akhter, AA Alqarawi, G Singh, KF Almutairi, EF Abd_Allah Saudi Journal of Biological Sciences , 2021 2021.0 Citations: 53
Distribution and antimicrobial potential of endophytic fungi associated with ethnomedicinal plant Melastoma malabathricum L. VK Mishra, G Singh, AK Passari, MK Yadav, VK Gupta, BP Singh Journal of environmental biology 37 (2), 229 , 2016 2016.0 Citations: 46
Ethnobotanical survey of medicinal plants used in the management of cancer and diabetes G Singh, AK Passari, MD Momin, S Ravi, BP Singh, NS Kumar J Tradit Chin Med 40 (6), 1007-1017 , 2020 2020.0 Citations: 40
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In Vivo Studies of Inoculated Plants and In Vitro Studies Utilizing Methanolic Extracts of Endophytic Streptomyces sp. Strain DBT34 Obtained from Mirabilis jalapa L. Exhibit … AK Passari, VV Leo, G Singh, L Samanta, H Ram, CN Siddaiah, ... International journal of molecular sciences 21 (19), 7364 , 2020 2020.0 Citations: 34
Improvements in HOMA indices and pancreatic endocrinal tissues in type 2-diabetic rats by DPP-4 inhibition and antioxidant potential of an ethanol fruit extract of Withania … H Ram, P Kumar, A Purohit, P Kashyap, S Kumar, S Kumar, G Singh, ... Nutrition & metabolism 18 (1), 1-17 , 2021 2021.0 Citations: 31
Ameliorations in dyslipidemia and atherosclerotic plaque by the inhibition of HMG-CoA reductase and antioxidant potential of phytoconstituents of an aqueous seed extract of … J Charan, P Riyad, H Ram, A Purohit, S Ambwani, P Kashyap, G Singh, ... PloS one 17 (3), e0264646 , 2022 2022.0 Citations: 22
DPP-4 inhibition mediated antidiabetic potential of phytoconstituents of an aqueous fruit extract of Withania coagulans (Stocks) Dunal: in-silico, in-vitro and in-vivo assessments P Kumar, H Ram, C Kala, P Kashyap, G Singh, C Agnihotri, BP Singh, ... Journal of Biomolecular Structure and Dynamics, 1-23 , 2022 2022.0 Citations: 17
Management of Fungal Pathogens in Pulses BP Singh, G Singh, K Kumar, SC Nayak, N Srinivasa Citations: 17
HMG‐CoA reductase inhibition medicated hypocholesterolemic and antiatherosclerotic potential of phytoconstituents of an aqueous pod extract of Prosopis cineraria (L.) Druce: In … N Jaipal, H Ram, J Charan, A Dixit, G Singh, BP Singh, A Kumar, ... eFood 3 (6), e42 , 2022 2022.0 Citations: 12
Microbiome of Pukzing Cave in India shows high antimicrobial activity against plant and animal pathogens MA Iquebal, AK Passari, J Jagannadham, F Ahmad, VV Leo, G Singh, ... Genomics , 2021 2021.0 Citations: 11
Streptococcus pneumoniae biofilms and human infectious diseases: A review R Zomuansangi, PK Singh, BP Singh, G Singh, P Deka, JJ Song, ... Understanding Microbial Biofilms, 475-483 , 2023 2023.0 Citations: 7
Saccharomyces and Their Potential Applications in Food and Food Processing Industries VV Leo, V Viswanath, P Deka, DR Ramji, L Pachuau, W Carrie, Y Malvi, ... Industrially Important Fungi for Sustainable Development, 393-427 , 2021 2021.0 Citations: 7
Author Correction: Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production AK Passari, VK Mishra, G Singh, P Singh, B Kumar, VK Gupta, RK Sarma, ... Scientific reports 8 (1), 1-1 , 2018 2018.0 Citations: 7
