Leila Gharehdaghi

Verified @znu.ac.ir

Animal Science Research Department
Assistant Professor Animal Science Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Tabriz, Iran.

Leila Gharehdaghi


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RESEARCH, TEACHING, or OTHER INTERESTS

Animal Science and Zoology, Insect Science
3

Scopus Publications

29

Scholar Citations

2

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • Evaluating the possibility of horizontal transfer of small RNAs from Sidr pollen to honey bee and their interaction in the host cell
    Leila Gharehdaghi, Taher Harkinezhad, Gholamhosein Tahmasbi
    Agricultural Biotechnology Journal, 2023
    Objective The existence of molecular relationship between different organisms through diet-derived plant miRNAs is one of the most challenging debates of the last decade. Considering the mutual relationship between plants and pollinating insects, including honey bee as the most important pollinating insect, the aim of the present study is to investigate the existence of molecular relationship in the interaction between plants and honey bee through plant-derived xenomiRs can be very important and create a new perspective of the beneficial effects of this relationship. Materials and methods Pollen was collected by pollen trap device from honey bee colonies that were located near Sidr trees. Honey bees were fed under controlled conditions in two groups (fed by Sidr pollen as treatment and fed by sugar syrup as control). Following the feeding experiments, the bees were anesthetized using cold and their midgut tissue was collected and used for RNA extraction. After the Small RNA sequencing of the samples, the identification of pollen miRNAs and their tracking in the bee body was done using different bioinformatics analysis. Finally, the target genes of the detected plant miRNAs in the bee body and the molecular pathway involved by them were determined. Results The results of bioinformatics analysis indicate the detection of eleven plant miRNAs including miR-148a, miR-26a, miR-21-5p, miR-143, miR-27a,miR-203, let-7g, miR-126, miR-206, miR-30d and miR-199b into the tissue of honey bees fed by Sidr pollen. miRNAs participate in various biological processes through their target genes. In the present study, 99 target genes for the detected plant miRNAs were predicted in honey bee genome. The result of KEGG pathway analysis showed that target genes are significantly involved in 23 different biological pathways. Conclusions The result of the current study clearly present the role of plant-derived xenomiRs in the regulation of honey bee gene expression. Therefore on one hand, these findings extend our understanding of the molecular interaction between honey bees and flowering plants, and on the other hand, it can be used as a practical road map for breeding studies in order to improve honey production and deal with diseases related to bees, as the most important pollinating insects in the 21st century’s agriculture.
  • Diet-derived transmission of MicroRNAs from host plant into honey bee Midgut
    Leila Gharehdaghi, Mohammad Reza Bakhtiarizadeh, Kang He, Taher Harkinezhad, Gholamhosein Tahmasbi, Fei Li
    BMC Genomics, 2021
    Background MicroRNA (miRNA) is a class of small noncoding RNAs, which targets on thousands of mRNA and thus plays important roles in many biological processes. It has been reported that miRNA has cross-species regulation functions between parasitoid-host, or plant-animal, etc. For example, several plant miRNAs enter into the honey bees and regulate gene expression. However, whether cross-species regulation function of miRNAs is a universal mechanism remains a debate question. Results We have evaluated transmission of miRNAs from sunflower and sedr plants into the midgut of honey bee using RNA-Seq analyses complemented with confirmation by RT-qPCR. The results showed that at least 11 plant miRNAs were found in the midgut of honey bee feeding by sunflower and sedr pollen. Among which, nine miRNAs, including miR-30d, miR-143, miR-148a, miR-21, let-7 g, miR-26a, miR-126, miR-27a, and miR-203, were shared between the sunflower- and sedr-fed honey bees, suggesting they might have essential roles in plant-insect interactions. Moreover, existence of these co-shared miRNAs presents a strong evidence to support the successful transmission of miRNAs into the midgut of the insect. In total, 121 honeybee mRNAs were predicted to be the target of these 11 plant-derived miRNAs. Interestingly, a sedr-derived miRNA, miR-206, targets on 53 honeybee genes. Kyoto Encyclopedia of Genes and Genome (KEGG) analyses showed that these target genes are significantly involved in hippo signaling pathway-fly, Wnt signaling pathway, and N-Glycan biosynthesis. Conclusions In summary, these results provide evidence of cross-species regulation function of miRNA between honeybee and flowering host plants, extending our understanding of the molecular interactions between plants and animals.
  • Identification of novel SNP in caprine β-lactoglobulin gene
    LEILA GHAREDAGHI, HOSEIN MORADI SHAHRBABAK, MOSTAFA SADEGHI
    Journal of Genetics, 2016

RECENT SCHOLAR PUBLICATIONS

  • Novel Strategies for Colony Management Under Heat Stress Conditions: From Laboratory Findings to Apiary Implementation
    S Parichehreh, L Gharehdaghi
    Honeybee Science Journal 16 (31) , 2026
    2026
  • A Review of the Role of Genetics in Honeybee Social Behaviors: From Colony Hygiene to the Waggle Dance
    L Gharehdaghi, F Parnian-khajehdizaj, M Pasandideh
    Honeybee Science Journal 16 (31) , 2026
    2026
  • From nutrition to disease: the challenges facing honey bees (Apis mellifera) in today's world
    L Gharehdaghi, A Rahimi, F Parnian_Khajehdizaj
    Honeybee Science Journal 16 (31) , 2026
    2026
  • Genetic achievements to identify molecular and neural mechanisms related to social behaviors in honey bees
    L Gharehdaghi, M Pasandideh
    Honeybee Science Journal 16 (30), 2-10 , 2025
    2025
  • Unraveling biological secrets: the genomic role of honeybee in understanding of its social life
    L Gharehdaghi, S Parichehreh Dizji, G Elyasi Zarringhabaie
    Honeybee Science Journal 15 (29), 24-31 , 2025
    2025
  • A Study on the Possibility of Molecular Communication between Honey Bees and Sunflower through the Interspecies Transfer of Small RNAs
    L Gharehdaghi, G Tahmasbi, T Harkinezhad
    Research On Animal Production 15 (4), 117-128 , 2024
    2024
  • Diet-derived transmission of plant small RNA: A novel perspective in unraveling the biological and medicinal properties of honey
    L Gharehdaghi, G Elyasi Zarringhabaie, F Parnian-khajehdizaj
    Honeybee Science Journal 15 (28), 28-35 , 2024
    2024
  • Evaluating the possibility of horizontal transfer of small RNAs from Sidr pollen to honey bee and their interaction in the host cell
    L Gharehdaghi, T Harkinezhad, G Tahmasbi
    Agricultural Biotechnology Journal 15 (2), 157-180 , 2023
    2023
  • ارزیابی امکان انتقال افقی RNA کوچک از گرده کنار (سدر) به زنبور عسل و برهمکنش آن‌ها در سلول میزبان ‎
    قره داغی, هرکی نژاد, محمد طاهر, طهماسبی ‎
    مجله بیوتکنولوژی کشاورزی 15 (2), 157-180 , 2023 ‎
    2023
  • کوچک از گرده کنار) سدر (به زنبور عسل و برهمکنش RNA ارزیابی امکان انتقال افقی آنها در سلول میزبان ‎
    لیلا قرهداغی, طاهر هرکینژاد, غلامحسین طهماسبی ‎
    Agricultural Biotechnology Journal 15 (2) , 2023
    2023
  • Diet-derived transmission of MicroRNAs from host plant into honey bee Midgut
    L Gharehdaghi, MR Bakhtiarizadeh, K He, T Harkinezhad, G Tahmasbi, ...
    BMC genomics 22 (1), 587 , 2021
    2021
    Citations: 12
  • Morphological diversity of Iranian small honey bee (Apis Florea) population based Principal component analysis and minimum variance methods
    MBFALGH S.S. Fatemi , T. Harkinezhad
    Livestock Research (LR) 6 (No.1&2), 15-25 , 2017
    2017
  • Identification of novel SNP in caprine β -lactoglobulin gene
    L Gharedaghi, HM Shahrbabak, M Sadeghi
    Journal of genetics 95 (3), 485-490 , 2016
    2016
    Citations: 14
  • Study of Polymorphism of β-Lactoglobulin Gene in Exon 7 and its Association with Milk Production Traits in Mahabadi Goats Using PCR-SSCP
    L Gharedaghi, M Sadeghi, H Moradi-Shahrebabak, M Ganjkhanlou
    Research on Animal Production 6 (11), 120-125 , 2015
    2015
    Citations: 2
  • STUDY OF THE POLYMORPHISM AT THE EXON 4 OF IGF-I GENE AND ITS ASSOCIATION WITH MILK PRODUCTION AND GROWTH TRAITS IN MAHABADI GOATS USING PCR-SSCP
    L Gharedaghi, SH MORADI, M Sadeghi, M Ganjkhanlou
    Agricultural Biotechnology Journal 6 (2), 133-145 , 2014
    2014
    Citations: 1

MOST CITED SCHOLAR PUBLICATIONS

  • Identification of novel SNP in caprine β -lactoglobulin gene
    L Gharedaghi, HM Shahrbabak, M Sadeghi
    Journal of genetics 95 (3), 485-490 , 2016
    2016
    Citations: 14
  • Diet-derived transmission of MicroRNAs from host plant into honey bee Midgut
    L Gharehdaghi, MR Bakhtiarizadeh, K He, T Harkinezhad, G Tahmasbi, ...
    BMC genomics 22 (1), 587 , 2021
    2021
    Citations: 12
  • Study of Polymorphism of β-Lactoglobulin Gene in Exon 7 and its Association with Milk Production Traits in Mahabadi Goats Using PCR-SSCP
    L Gharedaghi, M Sadeghi, H Moradi-Shahrebabak, M Ganjkhanlou
    Research on Animal Production 6 (11), 120-125 , 2015
    2015
    Citations: 2
  • STUDY OF THE POLYMORPHISM AT THE EXON 4 OF IGF-I GENE AND ITS ASSOCIATION WITH MILK PRODUCTION AND GROWTH TRAITS IN MAHABADI GOATS USING PCR-SSCP
    L Gharedaghi, SH MORADI, M Sadeghi, M Ganjkhanlou
    Agricultural Biotechnology Journal 6 (2), 133-145 , 2014
    2014
    Citations: 1
  • Novel Strategies for Colony Management Under Heat Stress Conditions: From Laboratory Findings to Apiary Implementation
    S Parichehreh, L Gharehdaghi
    Honeybee Science Journal 16 (31) , 2026
    2026
  • A Review of the Role of Genetics in Honeybee Social Behaviors: From Colony Hygiene to the Waggle Dance
    L Gharehdaghi, F Parnian-khajehdizaj, M Pasandideh
    Honeybee Science Journal 16 (31) , 2026
    2026
  • From nutrition to disease: the challenges facing honey bees (Apis mellifera) in today's world
    L Gharehdaghi, A Rahimi, F Parnian_Khajehdizaj
    Honeybee Science Journal 16 (31) , 2026
    2026
  • Genetic achievements to identify molecular and neural mechanisms related to social behaviors in honey bees
    L Gharehdaghi, M Pasandideh
    Honeybee Science Journal 16 (30), 2-10 , 2025
    2025
  • Unraveling biological secrets: the genomic role of honeybee in understanding of its social life
    L Gharehdaghi, S Parichehreh Dizji, G Elyasi Zarringhabaie
    Honeybee Science Journal 15 (29), 24-31 , 2025
    2025
  • A Study on the Possibility of Molecular Communication between Honey Bees and Sunflower through the Interspecies Transfer of Small RNAs
    L Gharehdaghi, G Tahmasbi, T Harkinezhad
    Research On Animal Production 15 (4), 117-128 , 2024
    2024
  • Diet-derived transmission of plant small RNA: A novel perspective in unraveling the biological and medicinal properties of honey
    L Gharehdaghi, G Elyasi Zarringhabaie, F Parnian-khajehdizaj
    Honeybee Science Journal 15 (28), 28-35 , 2024
    2024
  • Evaluating the possibility of horizontal transfer of small RNAs from Sidr pollen to honey bee and their interaction in the host cell
    L Gharehdaghi, T Harkinezhad, G Tahmasbi
    Agricultural Biotechnology Journal 15 (2), 157-180 , 2023
    2023
  • ارزیابی امکان انتقال افقی RNA کوچک از گرده کنار (سدر) به زنبور عسل و برهمکنش آن‌ها در سلول میزبان ‎
    قره داغی, هرکی نژاد, محمد طاهر, طهماسبی ‎
    مجله بیوتکنولوژی کشاورزی 15 (2), 157-180 , 2023 ‎
    2023
  • کوچک از گرده کنار) سدر (به زنبور عسل و برهمکنش RNA ارزیابی امکان انتقال افقی آنها در سلول میزبان ‎
    لیلا قرهداغی, طاهر هرکینژاد, غلامحسین طهماسبی ‎
    Agricultural Biotechnology Journal 15 (2) , 2023
    2023
  • Morphological diversity of Iranian small honey bee (Apis Florea) population based Principal component analysis and minimum variance methods
    MBFALGH S.S. Fatemi , T. Harkinezhad
    Livestock Research (LR) 6 (No.1&2), 15-25 , 2017
    2017