Hamed Hamouda

@qibebt.cas.cn

Qingdao Institute of Bioenergy and Bioprocess technology



              

https://researchid.co/hamedhamouda
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Scopus Publications

Scopus Publications

  • Strategy for preparing of glucosinolate derivatives with outstanding functional activities based on myrosinase
    Jiaqi Wang, Hong Jiang, Suxue Chen, Yan Li, Hamed I. Hamouda, Mohamed A. Balah, Changhu Xue, and Xiangzhao Mao
    Elsevier BV

  • Advances in fucoidan and fucoidan oligosaccharides: Current status, future prospects, and biological applications
    Hamed I. Hamouda, Tang Li, Samah Shabana, Amr H. Hashem, and Heng Yin
    Elsevier BV

  • A Novel Thermostable Laminarinase with High Activity from Streptomyces albus and Its Catalytic Characteristics in Laminarin Degradation
    Yongyi Quan, Hong Jiang, Hamed I. Hamouda, Jiayu Li, Hengxin Tang, and Xiangzhao Mao
    American Chemical Society (ACS)
    Laminarin oligosaccharides (LOSs) degraded from laminarin present nutritional functions. Laminarinases with high activity and good stability are significant tools for LOS production. OUC-SaLam66, a novel GH128 laminarinase from Streptomyces albus, was heterologously expressed. OUC-SaLam66 harbored a significant activity advantage up to 2294.80 U/mg at 45 °C and pH 4.0; meanwhile, it could preserve 80% activity at 45 °C for 12 h, indicating good stability. Significantly, its residual activity was over 75% after incubating at 100 °C for 1 h. Differential scanning calorimetry and molecular dynamic modeling revealed that its melting point and RMSD average point were 114.83 °C and 0.125 nm at 100 °C, respectively, which further proved its superior stability. Its apparent Km and Vmax against laminarin were 6.437 mM and 2.5 μg/min/mg, respectively. Hydrolysis products were mainly composed of laminaritriose and laminaribiose. The high activity and thermostability of OUC-SaLam66 make it a promising candidate for LOS preparation.

  • Mining of Novel Myrosinase with High Activity Based on Sequence and Structure Clustering for Efficient Preparation of Sulforaphane
    Jiaqi Wang, Hong Jiang, Suxue Chen, Yan Li, Zhe Wang, Hamed I. Hamouda, Mohamed A. Balah, Changhu Xue, and Xiangzhao Mao
    American Chemical Society (ACS)
    Sulforaphane has garnered significant research attention owing to its potent and promising biological activities. Mining the highly active myrosinase is the key to preparing sulforaphane. In this study, a novel myrosinase, designated Semyr, was identified from Serratia plymuthica through sequence and structural clustering analysis. The enzyme was heterologously expressed in Escherichia coli, demonstrating a sinigrin hydrolysis activity of 110.48 U/mg, which constitutes the highest recombinant myrosinase activity reported to date. A reaction system was established to prepare sulforaphane. 60 U of myrosinase was added to 5 mL of substrate, yielding 15.39 mg of sulforaphane per gram of broccoli seeds after 20 min at 40 °C and pH 6.0, with a conversion rate of 96.50%. Concurrently, the highest productivity of 5.55 μmol/g·min for sulforaphane was achieved after 15 min. Thus, Semyr serves as a valuable biocatalytic tool for the efficient preparation of sulforaphane.

  • Expression and characterization of a novel GH128 laminarinase OUC-BsLam26 harboring both hydrolyzing and transferring activities
    Yueyang Dong, Yongyi Quan, Hong Jiang, Hamed I. Hamouda, Zewei Lu, Yimiao Chen, Chengqiang Li, and Xiangzhao Mao
    Tsinghua University Press

  • Enhanced antimicrobial efficacy of hydroxyapatite-based composites for healthcare applications
    Maher Hassanain, Hamdy Maamoun Abdel-Ghafar, Hamed I. Hamouda, Fouad I. El-Hosiny, and Emad M. M. Ewais
    Springer Science and Business Media LLC
    AbstractHydroxyapatite (HAp) and hydroxyapatite-based materials show promising potential in the healthcare sector due to their distinctive properties such as biocompatibility, antimicrobial efficacy, non-toxicity, and robust mechanical characteristics. This makes HAp materials play an important role in hindering infection spreading in healthcare provider institutions. This study assesses the antimicrobial efficacy of the developed hydroxyapatite-based composites incorporating copper, zinc, and silver nanoparticles. The synthesized HAp and its modified composite variants (Cu/HAp, Zn/HAp, and Ag/HAp) with varying ratios ranging from 0 to 15% (wt) were characterized using XRD, XPS, SEM, and TEM analyses. Furthermore, the antibacterial and antifungal properties of the synthesized HAp and HAp-based composites were evaluated. The antibacterial effectiveness of the HAp and its composites was evaluated using a modified disc diffusion test, where the resulting inhibition zones on the agar surface were observed. All the HAp and HAp-based composites (HAp, Cu/HAp, Zn/HAp, and Ag/HAp materials) elicited in the formation of inhibitory zones. The most substantial inhibition values were observed for the 5% Ag/HAp formulation, with values of 19.7 and 13.8, against E. coli and S. aureus, respectively. The 5% Ag/HAp concentration may strike an ideal balance, providing high antimicrobial activity without adverse effects on biocompatibility or material stability. These findings underscore the recommendation of the proposed HAp-based composites for infection control measures through their application on medical instruments, textiles, healthcare personnel attire, and patient garments.

  • Cryogel with Modular and Clickable Building Blocks: Toward the Ultimate Ideal Macroporous Medium for Bacterial Separation
    Xiaomeng Yan, Fayi Wei, Jinpeng Gou, Mingbo Ji, Hamed I. Hamouda, Changhu Xue, and Hongwei Zheng
    American Chemical Society (ACS)
    The lack of practical platforms for bacterial separation remains a hindrance to the detection of bacteria in complex samples. Herein, a composite cryogel was synthesized by using clickable building blocks and boronic acid for bacterial separation. Macroporous cryogels were synthesized by cryo-gelation polymerization using 2-hydroxyethyl methacrylate and allyl glycidyl ether. The interconnected macroporous architecture enabled high interfering substance tolerance. Nanohybrid nanoparticles were prepared via surface-initiated atom transfer radical polymerization and immobilized onto cryogel by click reaction. Alkyne-tagged boronic acid was conjugated to the composite for specific bacteria binding. The physical and chemical characteristics of the composite cryogel were analyzed systematically. Benefitting from the synergistic, multiple binding sites provided by the silica-assisted polymer, the composite cryogel exhibited excellent affinity toward S. aureus and Salmonella spp. with capacities of 91.6 × 107 CFU/g and 241.3 × 107 CFU/g in 0.01 M PBS (pH 8.0), respectively. Bacterial binding can be tuned by variations in pH and temperature and the addition of monosaccharides. The composite was employed to separate S. aureus and Salmonella spp. from spiked tap water, 40% cow milk, and sea cucumber enzymatic hydrolysate, which resulted in high bacteria separation and demonstrated remarkable potential in bacteria separation from food samples.

  • In Situ Preparation of Star-Shaped Protein-“Smart” Polymer Conjugates with pH and Thermo-Dual Responsibility for Bacterial Separation
    Fayi Wei, Hongwei Zheng, Chao Gao, Jiaojiao Tian, Jinpeng Gou, Hamed I. Hamouda, and Changhu Xue
    American Chemical Society (ACS)
    To achieve effective separation and enrichment of bacteria, a novel synthetic scheme was developed to synthesize star-style boronate-functionalized copolymers with excellent hydrophilicity and temperature and pH responsiveness. A hydrophilic copolymer brush was synthesized by combining surface-initiated atom-transfer radical polymerization with amide reaction using bovine serum albumin as the core. The copolymer brush was further modified by introducing and immobilizing fluorophenylboronic acids through an amide reaction, resulting in the formation of boronate affinity material BSA@poly(NIPAm-co-AGE)@DFFPBA. The morphology and organic content of BSA@poly(NIPAm-co-AGE)@DFFPBA were systematically characterized. The BSA-derived composites demonstrated a strong binding capacity to both Gram-positive and Gram-negative bacteria. The binding capabilities of the affinity composite to Staphylococcus aureus and Salmonella spp. were 195.8 × 1010 CFU/g and 79.2 × 1010 CFU/g, respectively, which indicates that the novel composite exhibits a high binding capability to bacteria and shows a particularly more significant binding capacity toward Gram-positive bacteria. The bacterial binding of BSA@poly(NIPAm-co-AGE)@DFFPBA can be effectively altered by adjusting the pH and temperature. This study demonstrated that the star-shaped affinity composite had the potential to serve as an affinity material for the rapid separation and enrichment of bacteria in complex samples.

  • Characterization of a λ-Carrageenase Mutant with the Generation of Long-Chain λ-Neocarrageenan Oligosaccharides
    Zewei Lu, Hong Jiang, Dianqi Yang, Hengxin Tang, Hamed I. Hamouda, Tao Wang, and Xiangzhao Mao
    MDPI AG
    λ-carrageenan oligosaccharides can be widely applied in the food, pharmaceutical, medicine and cosmetic industries due to their abundant bioactivities, and they are important products for the high-value utilization of λ-carrageenan. However, oligosaccharides with different degrees of polymerization have different properties, and the final products of λ-carrageenase reported so far are mainly λ-neocarrabiose, λ-neocarratetraose and λ-neocarrahexaose without longer-chain oligosaccharides. Further research is consequently required. Herein, a mutant λ-carrageenase was constructed by deleting the pyrroloquinoline quinone-like domain of OUC-CglA derived from Maribacter vaceletii. Interestingly, it was discovered that the majority of final products of the mutant OUC-CglA-DPQQ were long-chain oligosaccharides with a polymerization degree of 10–20, which underwent significant changes compared to that of OUC-CglA. Additionally, without the pyrroloquinoline quinone-like domain, fewer inclusion bodies were produced throughout the expression process, and the yield of the λ-carrageenase increased about five-fold. However, compared to its parental enzyme, significant changes were made to its enzymatic properties. Its optimal temperature and pH were 15 °C and pH 7.0, and its specific activity was 51.59 U/mg. The stability of the enzyme decreased. Thus, it was found that the deleting domain was related to the formation of inclusion bodies, the stability of the enzyme, the activity of the enzyme and the composition of the products.

  • Development of an Anti-Organic Fouling Photothermal Membrane for Sustainable Freshwater Generation from Wastewater
    Hamdy Maamoun Abdel-Ghafar and Hamed I. Hamouda
    Springer Science and Business Media LLC

  • Catalytic properties characterization and degradation mode elucidation of a polyG-specific alginate lyase OUC-FaAly7
    Yimiao Chen, Fangfang Ci, Hong Jiang, Di Meng, Hamed I. Hamouda, Chunhui Liu, Yongyi Quan, Suxue Chen, Xinxue Bai, Zhaohui Zhang,et al.
    Elsevier BV

  • Green synthesis of bio-mediated silver nanoparticles from Persea americana peels extract and evaluation of their biological activities: In vitro and in silico insights
    Mohamed Yassin Ali, AboBaker Seddik Mahmoud, Mohnad Abdalla, Hamed I. Hamouda, Abeer S. Aloufi, Norah Saud Almubaddil, Yosra Modafer, Abdel-Moniem Sadek Hassan, Mostafa Abdel Makasoud Eissa, and Daochen Zhu
    Elsevier BV

  • Marine phospholipid nanoliposomes: A promising therapeutic approach for inflammatory bowel disease: Preparation, safety, and efficacy evaluation
    Samah Shabana, Hamed I. Hamouda, Alkassoumi Hassane Hamadou, Busati Ahmed, Zhe Chi, and Chenguang Liu
    Elsevier BV

  • Novel nanoblades of graphene oxide decorated with zinc oxide nanocomposite as a powerful anti-microbial active weapon
    Hamed I. Hamouda, Mohamed S. Selim, Shimaa A. Higazy, Samah Shabana, Zhifeng Hao, and Chenguang Liu
    Elsevier BV

  • High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase
    Lili Wang, Hamed I. Hamouda, Yueyang Dong, Hong Jiang, Yongyi Quan, Yimiao Chen, Yan Liu, Jiaqi Wang, Mohamed A. Balah, and Xiangzhao Mao
    Elsevier BV

  • Distinct roles of carbohydrate-binding modules in multidomain β-1,3–1,4-glucanase on polysaccharide degradation
    Hamed I. Hamouda, Yi-Xuan Fan, Mohnad Abdalla, Hang Su, Ming Lu, and Fu-Li Li
    Springer Science and Business Media LLC



  • Biochemical Characterization of a Cold-Adapted λ-Carrageenase OUC-CglA from Maribacter vaceletii: An Efficient Tool for λ-Carrageenan Degradation
    Zewei Lu, Hong Jiang, Hamed I. Hamouda, Tao Wang, Yueyang Dong, and Xiangzhao Mao
    American Chemical Society (ACS)
    λ-Carrageenase with high activity is an effective and environmentally friendly tool enzyme for the preparation of λ-carrageenan oligosaccharides with various biological activities. Herein, a novel GH150 (glycoside hydrolases family 150) λ-carrageenase OUC-CglA from Maribacter vaceletii was heterologously expressed, purified, and characterized. The recombinant OUC-CglA performs strict selectivity toward λ-carrageenan with a specific activity of 418.7 U/mg under its optimal reaction conditions of 20 °C and pH 7.0. Additionally, OUC-CglA is a typical cold-adapted λ-carrageenase because it unfolds 90% and 63% of its maximum activity at 15 and 10 °C, respectively. The hydrolysis process suggests that OUC-CglA is an endotype λ-carrageenase with the final products consisting of λ-neocarrabiose, λ-neocarratetraose, λ-neocarrahexaose, and other long-chain λ-neocarrageenan oligosaccharides. As a result, high activity, cold-adaptation, and principal products of OUC-CglA make it a potential biocatalyst for the effective preparation of λ-carrageenan oligosaccharides.

  • Rhamnolipid-Coated Iron Oxide Nanoparticles as a Novel Multitarget Candidate against Major Foodborne E. coli Serotypes and Methicillin-Resistant S. aureus
    Mohamed Sharaf, Alaa H. Sewid, H. I. Hamouda, Mohamed G. Elharrif, Azza S. El-Demerdash, Afaf Alharthi, Nada Hashim, Anas Abdullah Hamad, Samy Selim, Dalal Hussien M. Alkhalifah,et al.
    American Society for Microbiology
    Antimicrobial resistance poses a great threat and challenge to humanity. Therefore, the search for alternative ways to target and eliminate microbes from plant, animal, and marine microorganisms is one of the world’s concerns today.

  • Plastic-Associated Microbial Communities in Aquaculture Areas
    Mohamed Mohsen, Chenggang Lin, Hamed I. Hamouda, Ahmed M. Al-Zayat, and Hongsheng Yang
    Frontiers Media SA
    Microorganisms colonize plastics in the aquatic environment but their composition on plastics used in aquaculture remains poorly studied. Microorganisms play a significant role in aquaculture in terms of water quality and the health of cultivated species. In the current study, we explored the composition of microorganisms on floating plastics and their surrounding water collected from ponds and open aquaculture areas. Using scanning electron microscopy, the diversity of microbial communities, primarily diatoms, and bacteria were identified on the plastic surfaces. Additionally, epifluorescence microscopy revealed that prokaryotes were colonized on all plastic samples from 0.1 to 29.27×103 cells/cm2, with a high abundance found in open aquaculture areas compared to ponds. Bacterial communities were characterized by 16S rRNA sequencing which showed that bacterial communities on plastics were dominated by Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria. The level of these microbial communities on the plastics differed from those found in the surrounding seawater samples and the abundance of potentially pathogenic bacteria was higher in plastics than in seawater samples. Moreover, hydrocarbon-degrading bacteria were more abundant in the investigated plastic samples than in the water samples. This study contributes to the knowledge regarding the plastisphere community in aquaculture.

  • Expression and Biochemical Characterization of a Novel Fucoidanase from Flavobacterium algicola with the Principal Product of Fucoidan-Derived Disaccharide
    Yanjun Qiu, Hong Jiang, Yueyang Dong, Yongzhen Wang, Hamed I. Hamouda, Mohamed A. Balah, and Xiangzhao Mao
    MDPI AG
    Fucoidan is one of the main polysaccharides of brown algae and echinoderm, which has nutritional and pharmacological functions. Due to the low molecular weight and exposure of more sulfate groups, oligo-fucoidan or fucoidan oligosaccharides have potential for broader applications. In this research, a novel endo-α-1,4-L-fucoidanase OUC-FaFcn1 which can degrade fucoidan into oligo-fucoidan was discovered from the fucoidan-digesting strain Flavobacterium algicola 12,076. OUC-FaFcn1 belongs to glycoside hydrolases (GH) family 107 and shows highest activity at 40 °C and pH 9.0. It can degrade the α-1,4 glycosidic bond, instead of α-1,3 glycosidic bond, of the fucoidan with a random tangent way to generate the principal product of disaccharide, which accounts for 49.4% of the total products. Therefore, OUC-FaFcn1 is a promising bio-catalyst for the preparation of fucoidan-derived disaccharide. These results further enrich the resource library of fucoidanase and provide the basis for the directional preparation of fucoidan-derived oligosaccharide with specific polymerization.

  • Biochemical Characterization of a Novel Myrosinase Rmyr from Rahnella inusitata for High-Level Preparation of Sulforaphene and Sulforaphane
    Lili Wang, Hong Jiang, Yanjun Qiu, Yueyang Dong, Hamed I. Hamouda, Mohamed A. Balah, and Xiangzhao Mao
    American Chemical Society (ACS)
    Myrosinase is a biotechnological tool for the preparation of sulforaphane and sulforaphene with a variety of excellent biological activities. In this study, a gene encoding the novel glycoside hydrolase family 3 (GH3) myrosinase Rmyr from Rahnella inusitata was heterologously expressed in Escherichia coli BL21 (DE3). The purified Rmyr shows the highest activity at 40 °C and pH 7.0; meanwhile, its half-life at 30 °C reaches 12 days, indicating its excellent stability. Its sinigrin-, glucoraphenin-, and glucoraphanin-hydrolyzing activities were 12.73, 4.81, and 6.99 U/mg, respectively. Rmyr could efficiently degrade the radish seed-derived glucoraphenin and the broccoli seed-derived glucoraphanin into sulforaphene and sulforaphane within 10 min with the highest yields of 5.07 mg/g radish seeds and 9.56 mg/g broccoli seeds, respectively. The highest conversion efficiencies of sulforaphane from glucoraphanin and sulforaphene from glucoraphenin reached up to 92.48 and 97.84%, respectively. Therefore, Rmyr is a promising and potent biocatalyst for efficient and large-scale preparation of sulforaphane and sulforaphene.

  • Preparation, urease inhibition mechanisms, and anti-Helicobacter pylori activities of hesperetin-7-rhamnoglucoside
    Mohamed Sharaf, Muhammad Arif, Hamed I. Hamouda, Sohaib Khan, Mohnad Abdalla, Samah Shabana, Hussein. E. Rozan, Tehsin Ullah Khan, Zhe Chi, and Chenguang Liu
    Elsevier BV

  • Design of lipid-based nanocarrier for drug delivery has a double therapy for six common pathogens eradication
    Mohamed Sharaf, H.I. Hamouda, Samah Shabana, Sohaib Khan, Muhammad Arif, Hussein. E. Rozan, Mohnad Abdalla, Zhe Chi, and Chenguang Liu
    Elsevier BV