Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation Jin Ye, Isabel A. Calvo, Itziar Cenzano, Amaia Vilas, Xabier Martinez-de-Morentin, Miren Lasaga, Diego Alignani, Bruno Paiva, Ana C. Viñado, Patxi San Martin-Uriz, Juan P. Romero, Delia Quilez Agreda, Marta Miñana Barrios, Ignacio Sancho-González, Gabriele Todisco, Luca Malcovati, Nuria Planell, Borja Saez, Jesper N. Tegner, Felipe Prosper, David Gomez-Cabrero Iscience, 2022 Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.
Missed Chopart dislocation. The importance of being aware of midtarsal injuries I. Sancho González, M. Menéndez García Anales Del Sistema Sanitario De Navarra, 2016 Chopart (midtarsal) joint dislocations are relatively rare but potentially serious injuries. Their low prevalence and the possible absence of evident radiological findings cannot justify misdiagnosis because an adequate and correct treatment is required to achieve a proper clinical outcome.A midtarsal joint dislocation in a 19-year-old woman is described, in which diagnosis was performed at 8 weeks of evolution. An open reduction was performed by a double approach (medial and lateral) and a Kirschner wire joint stabilization. A tone-year of follow-up, loss of reduction was not observed and the patient was pain-free, although she referred to functional limitation when running.Besides describing the treatment of this particular injury, this study is aimed at increasing the level of clinical suspicion in order to avoid misdiagnosis such as occurred in our case.
RECENT SCHOLAR PUBLICATIONS
2025 ICM: Debridement, Antibiotics, and Implant Retention V Artyukh, GC Babis, J Baeza-Oliete, N Budhiparama, F Buttacavoli, ... JOURNAL OF ARTHROPLASTY 41 (1), S297-S325 , 2025 2025
2025 ICM: debridement, antibiotics, and implant retention J Cashman, SMJ Mortazavi, PF Indelli, S Rele, C Haasper, F Yildiz, ... The Journal of Arthroplasty 41 (1), S297-S325 , 2025 2025 Citations: 8
Toward a clinical algorithm for the detection of periprosthetic joint infections using targeted NGS A Uribarri, L Henriquez, I Beguiristain, I Rodriguez, M Alfaro, I Sancho, ... Diagnostic Microbiology and Infectious Disease, 117133 , 2025 2025 Citations: 1
Low-grade infections as a potential contributor of arthrofibrosis following total knee arthroplasty L Henríquez, A Uribarri, I Sancho, ME Portillo Journal of Bone and Joint Infection 10 (4), 303-307 , 2025 2025
Microbiological Profile and Resistance Patterns in Periprosthetic Joint Infections: A Regional Multicenter Study in Spain L Henriquez, A Uribarri, I Beguiristain, I Sancho, C Ezpeleta Baquedano, ... Microbiology Research 16 (7), 142 , 2025 2025
Caracterización de la reinfección de prótesis articular tras recambio en 2 tiempos (infección de la prótesis del 2. o tiempo). Un estudio multicéntrico JMB Allende, J Gómez-Junyent, LS Redó, D Rodríguez-Pardo, ÓM Rubio, ... Enfermedades Infecciosas y Microbiología Clínica 42 (7), 354-360 , 2024 2024
Description of reinfection of joint prosthesis after 2-stage replacement (infection of the 2nd stage prosthesis): A multicenter study JMB Allende, J Gómez-Junyent, LS Redó, D Rodríguez-Pardo, ÓM Rubio, ... Enfermedades infecciosas y microbiologia clinica (English ed.) 42 (7), 354-360 , 2024 2024 Citations: 3
Bone marrow spatial transcriptomics reveals a myeloma cell architecture with dysfunctional T-cell distribution, neutrophil traps, and inflammatory signaling L Sudupe, E Muiños-Lopez, AR Lopez-Perez, A Vilas-Zornoza, S Sarvide, ... bioRxiv, 2024.07. 03.601833 , 2024 2024 Citations: 8
Spatial Transcriptomics Reveals a Myeloma Cell Architecture with Dysfunctional T-Cell Distribution, Neutrophil Traps, and Inflammatory Signaling L Sudupe, E Muinos-Lopez, AR Lopez-Perez, A Vilas-Zornoza, S Sarvide, ... Cold Spring Harbor Laboratory , 2024 2024
Impact of antibiotic prophylaxis in second-stage surgery in joint prosthesis infection treated with two-stage exchange. A multicenter case–control study JM Barbero Allende, E Fernández Antón, J Gómez-Junyent, L Sorlí Redó, ... European Journal of Clinical Microbiology & Infectious Diseases 43 (7), 1319 … , 2024 2024
Transcriptional Characterization of the Stromal and Endothelial Bone Marrow Microenvironment during Progression from MGUS to Multiple Myeloma I Cenzano, M Cocera, AI Bantan, M Larrayoz, A Vilas, P San-Martin, ... Cold Spring Harbor Laboratory , 2024 2024 Citations: 1
Correction to: Should We Use Rifampicin in Periprosthetic Joint Infections Caused by Staphylococci When the Implant Has Been Exchanged? A Multicenter Observational Cohort Study TS Kramer, A Soriano, S Tedeschi, A Chen, P Tattevin, E Senneville, ... 2024
Transcriptional remodeling of the stromal and endothelial microenvironment in MGUS to multiple myeloma progression I Cenzano, M Cócera, A Bantan, M Larrayoz, A Vilas-Zornoza, ... bioRxiv 4, 589777 , 2024 2024 Citations: 5
Infección protésica articular. Avances diagnósticos y selección de candidatos en la estrategia de retención del implante I Sancho González Upna , 2024 2024
Combined single-cell and spatial transcriptomics unveil the complex organization of the non-immune human bone marrow microenvironment during aging I Cenzano, M Cocera, R Lehmann, J Ye, A Vilas-Zornoza, P San-Martin, ... Blood 142 (Supplement 1), 2706-2706 , 2023 2023 Citations: 1
Should we use rifampicin in periprosthetic joint infections caused by staphylococci when the implant has been exchanged? A multicenter observational cohort study TS Kramer, A Soriano, S Tedeschi, AF Chen, P Tattevin, E Senneville, ... Open forum infectious diseases 10 (10), ofad491 , 2023 2023 Citations: 15
Direct prosthetic joint infection diagnosis from sonication fluid inoculated in blood culture bottles by direct MALDI-TOF mass spectrometry I Beguiristain, L Henriquez, I Sancho, C Martin, A Hidalgo-Ovejero, ... Diagnostics 13 (5), 942 , 2023 2023 Citations: 16
Advances in the microbiological diagnosis of prosthetic joint infections ME Portillo, I Sancho Diagnostics 13 (4), 809 , 2023 2023 Citations: 36
Redo DAIR: the game is seldom worth the candle I Sancho, I Otermin-Maya, J Gutiérrez-Dubois, I Aláez, J Librero, ... Antibiotics 12 (1), 18 , 2022 2022 Citations: 4
Accuracy of a novel preoperative failure risk model for debridement antibiotics and implant retention (DAIR) in acute prosthetic joint infection I Sancho, I Otermin-Maya, J Gutiérrez-Dubois, I Alaez, Á Hidalgo-Ovejero, ... Diagnostics 12 (9), 2097 , 2022 2022 Citations: 10
MOST CITED SCHOLAR PUBLICATIONS
Advances in the microbiological diagnosis of prosthetic joint infections ME Portillo, I Sancho Diagnostics 13 (4), 809 , 2023 2023 Citations: 36
Upper extremity deep vein thrombosis in a triathlete: again intense endurance exercise as a thrombogenic risk I Sancho-González, MV Bonilla-Hernández, D Ibañez-Muñoz, ... The American journal of emergency medicine 35 (5), 808. e1-808. e3 , 2017 2017 Citations: 19
Direct prosthetic joint infection diagnosis from sonication fluid inoculated in blood culture bottles by direct MALDI-TOF mass spectrometry I Beguiristain, L Henriquez, I Sancho, C Martin, A Hidalgo-Ovejero, ... Diagnostics 13 (5), 942 , 2023 2023 Citations: 16
Should we use rifampicin in periprosthetic joint infections caused by staphylococci when the implant has been exchanged? A multicenter observational cohort study TS Kramer, A Soriano, S Tedeschi, AF Chen, P Tattevin, E Senneville, ... Open forum infectious diseases 10 (10), ofad491 , 2023 2023 Citations: 15
Missed Chopart dislocation. The importance of being aware of midtarsal injuries. S González Anales del sistema sanitario de Navarra 39 (1), 153-158 , 2016 2016 Citations: 12
Accuracy of a novel preoperative failure risk model for debridement antibiotics and implant retention (DAIR) in acute prosthetic joint infection I Sancho, I Otermin-Maya, J Gutiérrez-Dubois, I Alaez, Á Hidalgo-Ovejero, ... Diagnostics 12 (9), 2097 , 2022 2022 Citations: 10
The efficacy of suppressive antibiotic treatment in patients managed non-operatively for periprosthetic joint infection and a draining sinus KJDF Lensen, R Escudero-Sanchez, J Cobo, R Trebše, C Gubavu, ... Journal of bone and joint infection 6 (7), 313-319 , 2021 2021 Citations: 10
2025 ICM: debridement, antibiotics, and implant retention J Cashman, SMJ Mortazavi, PF Indelli, S Rele, C Haasper, F Yildiz, ... The Journal of Arthroplasty 41 (1), S297-S325 , 2025 2025 Citations: 8
Bone marrow spatial transcriptomics reveals a myeloma cell architecture with dysfunctional T-cell distribution, neutrophil traps, and inflammatory signaling L Sudupe, E Muiños-Lopez, AR Lopez-Perez, A Vilas-Zornoza, S Sarvide, ... bioRxiv, 2024.07. 03.601833 , 2024 2024 Citations: 8
Transcriptional remodeling of the stromal and endothelial microenvironment in MGUS to multiple myeloma progression I Cenzano, M Cócera, A Bantan, M Larrayoz, A Vilas-Zornoza, ... bioRxiv 4, 589777 , 2024 2024 Citations: 5
Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation J Ye, IA Calvo, I Cenzano, A Vilas, X Martinez-de-Morentin, M Lasaga, ... Iscience 25 (5) , 2022 2022 Citations: 5
Redo DAIR: the game is seldom worth the candle I Sancho, I Otermin-Maya, J Gutiérrez-Dubois, I Aláez, J Librero, ... Antibiotics 12 (1), 18 , 2022 2022 Citations: 4
Description of reinfection of joint prosthesis after 2-stage replacement (infection of the 2nd stage prosthesis): A multicenter study JMB Allende, J Gómez-Junyent, LS Redó, D Rodríguez-Pardo, ÓM Rubio, ... Enfermedades infecciosas y microbiologia clinica (English ed.) 42 (7), 354-360 , 2024 2024 Citations: 3
Toward a clinical algorithm for the detection of periprosthetic joint infections using targeted NGS A Uribarri, L Henriquez, I Beguiristain, I Rodriguez, M Alfaro, I Sancho, ... Diagnostic Microbiology and Infectious Disease, 117133 , 2025 2025 Citations: 1
Transcriptional Characterization of the Stromal and Endothelial Bone Marrow Microenvironment during Progression from MGUS to Multiple Myeloma I Cenzano, M Cocera, AI Bantan, M Larrayoz, A Vilas, P San-Martin, ... Cold Spring Harbor Laboratory , 2024 2024 Citations: 1
Combined single-cell and spatial transcriptomics unveil the complex organization of the non-immune human bone marrow microenvironment during aging I Cenzano, M Cocera, R Lehmann, J Ye, A Vilas-Zornoza, P San-Martin, ... Blood 142 (Supplement 1), 2706-2706 , 2023 2023 Citations: 1
2025 ICM: Debridement, Antibiotics, and Implant Retention V Artyukh, GC Babis, J Baeza-Oliete, N Budhiparama, F Buttacavoli, ... JOURNAL OF ARTHROPLASTY 41 (1), S297-S325 , 2025 2025
Low-grade infections as a potential contributor of arthrofibrosis following total knee arthroplasty L Henríquez, A Uribarri, I Sancho, ME Portillo Journal of Bone and Joint Infection 10 (4), 303-307 , 2025 2025
Microbiological Profile and Resistance Patterns in Periprosthetic Joint Infections: A Regional Multicenter Study in Spain L Henriquez, A Uribarri, I Beguiristain, I Sancho, C Ezpeleta Baquedano, ... Microbiology Research 16 (7), 142 , 2025 2025
Caracterización de la reinfección de prótesis articular tras recambio en 2 tiempos (infección de la prótesis del 2. o tiempo). Un estudio multicéntrico JMB Allende, J Gómez-Junyent, LS Redó, D Rodríguez-Pardo, ÓM Rubio, ... Enfermedades Infecciosas y Microbiología Clínica 42 (7), 354-360 , 2024 2024
