Dafit Ariyanto
@una.ac.id
Department of Aquaculture, Faculty of Agriculture
Asahan University, Kisaran, North
Sumatera, Indonesia
Scopus Publications
Scopus Publications
Gunawan Widi Santosa, Ali Djunaedi, AB Susanto, Delianis Pringgenies, Dafit Ariyanto, and Aris Kabul Pranoton
College of Graduate Studies, Walailak University
Green macroalgae, known locally as Latoh, is one of the green seaweeds consumed by the local community in Jepara and is beneficial for health. This study explores the potential of secondary metabolites from seaweed and its symbiotic bacteria as natural food preservatives and antibacterials. Seaweed samples were collected from the seagrass ecosystem of Panjang Island, Jepara, Indonesia. Subsequently, the samples were subjected to scanning electron microscopy analysis, proximate analysis, phytochemical analysis, thin layer chromatography, and high-performance liquid chromatography for amino acid analysis. A sample was subjected to a multistage extraction process using n-hexane, ethyl acetate, and methanol (1:5 w/v), each for 24 h. Symbiotic bacteria from seaweed were isolated, and enzymatic (proteolytic, amylolytic, and cellulolytic) and antibacterial testing against pathogenic bacteria Staphylococcus aureus and Escherichia coli was conducted using the disc diffusion method. The selected bacteria were subjected to molecular identification. The research showed that Caulerpa lentillifera had an ash content of 3.24 %, protein content of 0.57 %, and fat content of 0.337 %. Phytochemical analysis shows that the sample contains flavonoids, steroids, and alkaloids. HPLC analysis reveals that Caulerpa lentillifera has the highest content of aspartic acid (relative area: 11.90 %), glutamic acid (relative area: 13.43 %), and alanine (relative area: 9.03 %). Caulerpa racemosa sample shows the highest detector response for glutamic acid (relative area: 12.19%), aspartic acid (relative area: 11.10 %), and alanine (relative area: 9.63 %). The results indicate that 14 bacterial isolates were successfully isolated, with 6 isolates from Caulerpa lentillifera and 8 isolates from Caulerpa racemosa, all exhibiting enzymatic and antibacterial abilities. The research results concluded that the Latoh seaweed species Caulerpa lentillifera and Caulerpa racemosa and their symbiotic bacteria have the potential to be used as food preservatives. HIGHLIGHTS HPLC analysis showed that Caulerpa lentillifera had the highest content of aspartic acid, glutamic acid, and alanine. Caulerpa racemosa also had high levels of glutamic acid, aspartic acid, and alanine The research successfully isolated 14 bacterial isolates, with 6 isolates from Caulerpa lentillifera and eight isolates from Caulerpa racemosa, all exhibiting enzymatic and antibacterial abilities Molecular identification revealed the presence of identified bacteria such as Pseudoalteromonas arabiensis, Pseudoalteromonas piscicida, Pseudoalteromonas shioyasakiensis, and Vibrio alginolyticus seaweed species Caulerpa lentillifera and Caulerpa racemosa, along with their symbiotic bacteria, have the potential to be used as natural food preservatives due to their antibacterial and enzymatic pro perties GRAPHICAL ABSTRACT
Ali Ridlo, Delianis Pringgenies, Ribka Anindita Br Perangin-angin, and Dafit Ariyanto
Universitas Airlangga
Highlight Research The methanolic extracts of salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins. Chlorophyll a, chlorophyll b, and carotenoids were most abundant in the ethyl acetate extracts of salinaand B. braunii. The maximum total phenolic content was observed in the n-hexane extract of salina(61.28 mg GAE/g sample) and the ethyl acetate extract of B. braunii (46.94 mg GAE/g sample). The n-hexane extracts exhibited the highest antioxidant activity, whereas saponins were most abundant in the methanol extracts. Abstract Microalgal species such as Dunaliella salina and Botryococcus braunii are reportedly rich in natural antioxidants and phytochemicals. This study aimed to determine the phytochemicals and the antioxidant activity of D. salina and B. braunii. Microalgal samples were obtained from the Brackish Water Cultivation Fisheries Center (BPBAP), Situbondo, East Java. The extracts were prepared using the multilevel maceration method. The antioxidant activity of the algal species was analyzed using 1,1-diphenyl-2-picrylhydraxyl (DPPH). Quantitative analysis revealed that D. salina and B. braunii contained antioxidants, indicated by the appearance of yellow spots on the purple background of the TLC plate. The n-hexane extract of D. salina exhibited the highest antioxidant activity with a half-maximal inhibitory concentration (IC50) of 443.28 ppm, 61.28 mg GAE/g sample of total phenolics, 0.106 mg/g of chlorophyll a, 0.165 mg/g of chlorophyll b, and 1,697 mol/g of carotenoids. In contrast, the ethyl acetate extract of B. braunii exhibited the highest antioxidant activity with an IC50 of 634.55 ppm, 46.94 mg GAE/g sample of total phenolics, 18.146 mg/g of chlorophyll a, 12.592 mg/g of chlorophyll b, and 4573 mol/g of carotenoids. The microalgal species used in this study exhibited extremely weak antioxidant activity.
Indah Listiana, Kordiyana K Rangga, Ayu Agustina, Indah Nurmayasari, Habibullah Jimad, and Dafit Ariyanto
Asian Economic and Social Society
This study explores the effect of field agricultural extension on rice farmers’ capacity levels and the factors that influence farmers' capacity. This research was conducted in Negeri Katon District in October-November 2021. The respondents were 62 heads of farmer groups in the district. This study used a survey method with a quantitative descriptive approach. The results showed that the effect of field agricultural extension on farmers’ capacity levels could be categorized as moderate, and the overall level of farmer capacity could be classified in the medium category. This is an interesting finding considering the daily activities of farmers consist of farming but their capacity is not yet high. This finding shows that farmers still need agricultural extension agents (AEAs) but the number of AEAs is still limited, meaning that extension activities do not run optimally. The factors that influence the level of farmer capacity are age, level of formal education, length of farming experience, land size, number of dependents, and the role of AEAs. The number and role of AEAs affect the capacity of extension workers; therefore, the number of AEAs must be increased.
R T A Pertiwi, D Ariyanto, Edward, M R Lessy, and B Putri
IOP Publishing
Abstract Fish organs can contain heavy metals. This study’s objective was to map the distribution of heavy metals in several fish organs.. The research was conducted in Kao Bay waters from April to September 2019. The samples obtained were prepared at the aquatic science laboratory of Khairun University and were analyzed at the Environmental Productivity Laboratory of IPB University Bogor using the AAS instrument to determine heavy metals. The heavy metals accumulation in Lethrinus sp fish meat was Zn > Cu > Mn > Pb > Ni > Hg > Cd, and in gill was Zn > Pb > Mn > Ni > Hg > Cd. The accumulation of heavy metals in Caranx ignobilis fish meat was Zn > Pb > Mn > Cu > Ni > Hg > Cd, whereas the accumulation in the gills was Zn > Pb > Ni > Mn > Cd > Hg > Cu. Zn accumulation was the highest heavy metal discovered in fish as a result.
D Pringgenies, D P Wijayanti, M A Rois, and D Ariyanto
IOP Publishing
Abstract Mangroves have many environmental, ecological, economic, health and industrial benefits. One of the applications of mangroves in industry is as a natural dye for textile materials. The aim of the study was to determine the potential application of mangrove extract as a natural dye for batik, to determine the pigment content of the test sample, and to test the fastness of natural color made from leaves and bark of Avicennia marina and Bruguier gymnorrhiza, at plant ages of 5 and 10 years. Sample collection was done by picking leaves and collecting the bark of Avicennia marina and Bruguier gymnorrhiza. Color fastness to detergent washing test, wet and dry rubbing test were carried out with a crockmeter, using the Staining Scale and Gray Scale standards. UV Vis Spectrophotometry Analysis was employed to determine the pigment content. The results showed that leaf and bark extracts of Avicennia marina and Bruguiera gymnorrhiza mangroves have the potential to be applied as natural dyes for batik. Mangrove natural dyes showed scores of 3, 4, and 5 on the fastness tests of dry rubbing, wet rubbing and detergent washing. These findings indicate that the natural dyes in this study have met the Indonesian National Standard, which requires a minimum score of 3.
Umroh Umroh, Suci Puspita Sari, Verry Andre Fabiani, Dafit Ariyanto, Aries Dwi Siswanto, and Nuryanto Nuryanto
Institute of Research and Community Services Diponegoro University (LPPM UNDIP)
The distribution and enrichment of heavy metals in sediments will affect the life of the organisms that lives in it. The purpose of this study is to explain the enrichment and contamination levels of heavy metals Cu and Pb in bulk sediments in the northern and southern parts of Bangka Island. This research was conducted in August - September 2019, using a purposive sampling method. Heavy metal analysis using the Inductively Coupled Plasma - Optical Emission Spectroscopy (ICP-OES) instrument. The results showed that the concentration of the heavy metal Pb in the sediment bulk of northern Bangka Island (Kelabat Bay) was higher than that of South Bangka Island. The concentration of heavy metal Cu at 8 (eight) sites is still below the quality standard (18.7 mg.kg-1) even if the heavy metal Pb at stations 3, 4, and 5 in the waters of the northern half of Bangka Island (Kelabat Bay) is reported to have exceeded the quality standard limit (30.2 mg.kg-1). The highest level of enrichment of heavy metal Pb occurs at station 4 with a value of EF_62.88, and is categorized as very high enrichment and a contamination factor (CF) value of 2.24 (medium category). This condition is due to station 4 being located in Kelabat Bay (semi-enclosed area) with unstable water conditions due to the influence of many activities. The results of this study can be used as input for local governments for the management of water areas on Bangka Island.
R T A Pertiwi, K H Iksan, and D Ariyanto
IOP Publishing
Abstract Heavy metals have a relationship with fish organs. The aim of this study was to determine the distribution of heavy metals in various organs of Gerres abbreviatus and Parastromateus niger. The research was conducted in Kao Bay waters from April to September 2019. Samples obtained were prepared at the aquatic science laboratory of Khairun University and were analyzed at the Environmental Productivity Laboratory of IPB University Bogor using the AAS instrument to determine heavy metals concentrations. The result of the heavy metals accumulation in Gerres abbreviatus meat was Zn > Cu > Pb > Ni > Hg > Mn > Cd. Meanwhile, the heavy metals accumulation in Roi fish (Parastromateus niger) meat was Zn > Mn > Ni > Hg > Pb > Cd > Cu.
Delianis Pringgenies, Wilis Ari Setyati, Ali Djunaedi, Rini Pramesti, Siti Rudiyanti, and Dafit Ariyanto
Universitas Airlangga
Highlight ResearchAntimicrobial potential against the test microbesRhizhopora mucronata isolate showed 95% homology with Bacillus subtilis, and 97% homology with Bacillus oceanisediminis,Acanthus ilicifolius isolate showed 96% homology with Paracoccus caeni, and 89% homology with Bacillus circulans. The study found 4 isolates with antimicrobial potency against MDR pathogenic microbes.The symbiont microbes taken from Rhizophora mucronata and Acanthus ilicifolius were determined to be of the genus Bacillus and Paracoccus AbstractAntimicrobial property of mangrove symbiont have the ability to fight Multi Drug Resistant bacteria which were Staphylococcus aureus, Escherichia coli, and Vibrio haryeyi. This study aimed to determine the potential of symbiont microbes from the root of Rhizopora mucronata and Acanthus iilicifolius as antimicrobial agents against multi-drug resistant (MDR) pathogenic microbes. This research was conducted during July to November 2020. The MDR bacteria were S. aureus, E. coli, and V. harveyi MDR test microbes. The symbiont microbes were identified through molecular analyses (PCR 16S rDNA). Isolation of symbiont microbes from R. mucronata resulted in 16 isolates, while isolation from A. iilicifolius resulted in 14 isolates. Based on the antimicrobial qualitative test against S. aureus, 8 out of 16 microbial isolates from R. mucronata were found to show antimicrobial properties. The testing of A. ilicifolius symbiont microbes against S. aureus showed 8 out of 14 isolates with antimicrobial properties. The test against E. coli resulted in 2 out of 16 microbial isolates from R. mucronata and 5 out of 14 isolates from A. ilicifolius with antimicrobial properties. The test against V. harveyi resulted in two out of 16 microbial isolates from R.mucronata and 4 out of 14 isolates from A. ilicifolius with antimicrobial properties. The quantitative test found 2 isolates from R. mucronta, namely isolates RM10 and RM12, with antimicrobial properties against MDR strain E. coli, with the best isolate being RM10, which produced 11.22 mm of inhibition zone diameter. Furthermore, the selection of isolates was based on the size of the inhibition zone, the clearness of the inhibition zone and the potential for antibacterial activity. Based on their overall antimicrobial potential against the test microbes, four isolates were selected. Molecular analyses of RM12 isolate showed 95% homology with Bacillus subtilis, of RM 10 isolate showed 97% homology with Bacillus oceanisediminis, of AC isolate showed 96% homology with Paracoccus caeni, and of AC 5 isolate showed 89% homology with Bacillus circulans. The study found four isolates with antimicrobial potency against MDR pathogenic microbes. The symbiont microbes taken from R. mucronata and A. ilicifolius were determined to be of the genus Bacillus and Paracoccus.
Delianis Pringgenies et al.
Egypts Presidential Specialized Council for Education and Scientific Research
C I Wahyudin, A Alfian, N Goo, V L Tanasale, M H Makaruku, A S Mahulette, W D Mariati, D Ariyanto, and K I Prahesti
IOP Publishing
Dafit Ariyanto, Dietriech G. Bengen, Tri Prartono, and Yusli Wardiatno
Egypts Presidential Specialized Council for Education and Scientific Research
Sri S. Ningsih, Dafit Ariyanto, Dian Puspitasari, Anuraga Jayanegara, Hamim Hamim, and Heru Gunawan
EDP Sciences
Amino acids are important components of mangrove plant metabolisms. The aim of this study was to determine the relationship of mangrove R. mucronata leaf colors to the amino acid content. This study was conducted between March to August 2019. The leaves were taken 50 g. The assessment of Amino acids was conducted using UPLC Waters Acquity Class H with PDA Detector. The principal component analysis (PCA) was used to determine the relationship analysis between R. mucronata leaf colors and amino acid concentrations. The largest content was L glutamic acid with a green leaf content of 6139.57 ± 694.17 mg/kg and yellow leaf content of 6105.013 ± 113.2058 mg/kg. The results of PCA 1 showed that the influential amino acids were L-threonine (0.94), L-tyrosine (0.96), L-alanine (0.92), L-vanin (0.93), and PCA 2 showed glutamic acid (0.91). These results indicated that the difference in mangrove leaf colors affects the amino acid contents in the leaves.
Most cocoa plantations have undergone changes and sustained damage due to climate change. The objectives of the present study were to identify sustainable cocoa farming strategies in the effort to overcome climate change effects in regencies; and to analyze sustainable business strategies in overcoming the effects of climate change; This study was conducted from June to August 2018 in Luwu and North Luwu Regencies, South Sulawesi Province. Data collection was carried out through interviews with 282 farmers and interviews conducted on five key informants. Descriptive statistical analysis was conducted in the form of a frequency distribution table using Excel program and SPSS version 24. Model implementation strategies in overcoming climate change effects were designed based on the SEM PLS 2. The results revealed that the effort to overcome climate change effects in the development of the cocoa agribusiness and the farmers’ social relationships were high, while preservation of environmental conditions was in the low category. Conclusion : Sustainable cocoa farming strategies in the effort to overcome climate change effects consisted of adaptation climate accompaniment extension strategies, increasing government support strategies, and farmer character improvement strategies in overcoming climate change effects.
Y. Rustandi
Blue Eyes Intelligence Engineering and Sciences Engineering and Sciences Publication - BEIESP
Islamic boarding school is one of the islamic educational institutions that grow fastly with diversity method in Indonesia This study is aimed to describe the effort to grow young entrepreneur at Islamic Boarding School al Ittifaq Ciwidey Bandung through agribusiness activity with a focus on student empowerment. The study was done at Al-Ittifaq Ciwidey Bandung by applying qualitative research approach. Informants were selected by means of purposive sampling and snowball sampling which were further classified into three groups (triangulation). Furthermore, data collection technic was conducted through in-depth interview, direct observation, documentation, and FGD (Focus Group Discussion). Data analysis technic was performed using interactive model. Regarding the effort to grow young entrepreneurs, result of the study shows that student at al Ittifaq Ciwidey Bandung involve in agribusiness activities such as: (1) the formation of Executive Board of Agribusiness Unit, (2) conducting technical practices directly in the field, (3) establishing Agribusiness Incubation Center (PIA), (4) establishing cooperation/partnership with various institutions, and (5) setting up agribusiness groups of students
Indah Listiana, Sumardjo Sumardjo, D. Sadono and P. Tjitropranoto
Blue Eyes Intelligence Engineering and Sciences Engineering and Sciences Publication - BEIESP
Low internet usage by extension agent, impact on the inhibition of the flow of information to the farmers and lead to the income received by farmers. In the digital era today, if the extension agent is not encouraged to use the internet then they will be left behind and will be left out by the farmers. This study aimed to (1) analyze the internet availability in Lampung Province (2) to analyze the level of internet usage for extension agent in Lampung and (3) to analyze factors influencing internet usage of extension agent. This research was conducted in Lampung Province with analysis unit of extension agent in the office of BP3K (Extension Agency of Agriculture, Fisheries, and Forestry) which has been facilitated by computer and internet network. Respondents consisted of 355 extension agent served in BP3K. Multiple Linear Regression Analysis was used to determine the effect of independent variables on the dependent variable. The availability of facilities and internet network in Extension Offices is not sufficient even extension agent used their private facilities. The Internet network had reached the remote areas very well in spreading and transmitting information. Almost all extension agent have internet-connected devices and internet-based applications. Extension agents facilitated by computer and internet network communicating using social media, however capabilities in using the internet to search information related agricultural technology remained low. The use of the internet by extension agent was influenced by the nature of internet innovation, the number of Internet-based applications and the amount of existed hardware.
Dafit Ariyanto, , Dietriech G Bengen, Tri Prartono, Yusli Wardiatno, , , and
Faculty of Environment and Resource Studies - Mahidol University