2004 - 2007 bachelor study programme, Masaryk university in Brno, Czech Republic, Faculty of Sciences, Molecular Biology and Genetics, topic: Role of arachidonic acid metabolites in carcinogenesis
2007 - 2009 masters study programme, Masaryk university in Brno, Czech Republic, Faculty of Sciences, Molecular Biology and Genetics, topic: Role of Studium úlohy proteinů c-Myb a Cox-2 ve střevních buňkách
2011 - 2016 doctoral study programme, Masaryk university in Brno, Czech Republic, Faculty of Sciences, Animal Physiology, topic: Hyaluronan based drug carrier systems
From ecology to evolution: plasmid- and colicin-mediated persistence of antibiotic-resistant Escherichia coli in gulls Michaela Ruzickova, Jana Palkovicova, Kristina Nesporova, Marketa Rysava, Rene Pariza, Simon Krejci, Ivan Literak, Monika Dolejska Msystems, 2026 Antimicrobial resistance (AMR) in wildlife is an emerging concern within the One Health concept. Gulls, due to their synanthropic behavior and long-distance migration, are recognized as vectors and secondary reservoirs of resistant bacteria. These birds can facilitate the environmental spread of resistant strains across ecosystem boundaries. Understanding their role in shaping microbial communities is essential for assessing the broader ecological impact. This study investigates the persistence and competitive dynamics of cephalosporin-resistant Escherichia coli in Caspian gulls ( Larus cachinnans ) captured at their breeding colony at a water reservoir and subsequently monitored in captivity for three months, representing the longest in vivo experiment of its kind conducted on wild birds. We observed sustained colonization and long-term shedding of resistant E. coli throughout the entire study, marking the longest documented carriage of resistant bacteria in wild birds to date. Notably, rapid dissemination of various E. coli sequence types (STs) with CTX-M-1 was observed, with ST11138 rapidly outcompeting other strains, including the initially dominant ST11893. Genomic analyses revealed that ST11138 harboured F24:A-:B1 and IncI1/ST3/CTX-M-1 plasmids encoding colicins and corresponding immunity genes, likely conferring a competitive advantage. Our findings underscore the role of bacteriocin-mediated interactions in shaping microbial communities and highlight the importance of plasmid-encoded traits in the persistence of resistant strains in wildlife. Importantly, our findings underscore the ecological novelty of longitudinal in vivo tracking of AMR persistence in natural hosts and highlight the need to consider ecological and microbiome-level interactions when assessing the environmental dimension of AMR under the One Health concept. IMPORTANCE Antimicrobial resistance (AMR) in wildlife is an emerging concern within the One Health framework, with gulls recognised as important vectors and secondary reservoirs of resistant bacteria. Due to their synanthropic behavior and long-distance migration, these birds can facilitate the spread of resistant strains across ecosystems. However, the role of wildlife in resistance dynamics remains underexplored, especially in long-term, natural settings. Our study is unique in its scope and duration, representing the longest in vivo experiment of its kind conducted on wild birds. By capturing these processes in live hosts under naturalistic conditions and across an extended period, our study provides rare and ecologically grounded insights into how AMR is maintained outside clinical or laboratory settings. Our findings show sustained colonisation and long-term shedding of resistant E. coli , with strain ST11138 outcompeting others. Genomic analyses reveal plasmid-encoded traits, highlighting the ecological and evolutionary mechanisms underlying resistance maintenance in wildlife.
Generation of Bioactive Stem Cell-Derived Secretome in 3D Bioreactor System: Towards Cell-Free Therapy in Veterinary Medicine Věra Daňková, Andrea Exnerová, Hana Vágnerová, Vojtěch Pavlík, Kristina Nešporová Biomolecules, 2026 Canine adipose-derived mesenchymal stem cells (cASC) are promising for regenerative veterinary medicine due to their immunomodulatory and reparative capacities. Three-dimensional (3D) culturing provides a more physiologically relevant environment than conventional two-dimensional (2D) monolayers, enhancing paracrine activity and therapeutic potential of mesenchymal stem cells (MSC). This study investigates the production and biological characterization of cASC secretome generated under hypoxic conditions with platelet lysate (PLT) supplementation, either in a 2D culture or in a stirred-tank 3D culture. Secretomes obtained from 3D cultures were compared with those from 2D cultures prepared under identical hypoxic and PLT-supplemented conditions. Quantitative analyses revealed enhanced secretion of key factors, including monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF), in 3D-derived secretomes. Functional in vitro assays demonstrated superior anti-inflammatory, pro-migratory, and antifibrotic effects of the 3D secretome, evidenced by nuclear factor kappa B (NF-κB) inhibition, increased fibroblast migration, and modulation of extracellular matrix gene expression. Additionally, the bioreactor system enabled consistent secretome production with reproducible biological activity. These findings indicate that 3D bioreactor cultivation under hypoxia with PLT supplementation can generate a biologically active secretome from canine adipose-derived stem cells, providing a promising basis for further exploration in veterinary regenerative applications.
Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis Bin Liu, Kaat Sondervorst, Kristina Nesporova, Karoline Faust, Sander K. Govers Communications Biology, 2025 The human gut is a densely populated environment where microbial cells face frequent nutrient fluctuations. While previous studies have identified changes in gene expression, protein concentrations, and metabolite levels, less is known about how gut bacteria respond to nutrient fluctuations at the cellular level. Here, we compared cell biological properties of B. thetaiotaomicron and R. intestinalis, two prevalent human gut microbiome members. Our comparison revealed distinct cellular properties of both bacteria during exponential growth in vitro, including a unique zonal peptidoglycan synthesis pattern in R. intestinalis. Upon nutrient depletion in stationary phase, we observed different cell morphological alterations, with B. thetaiotaomicron cells becoming wider and R. intestinalis cells shorter, accompanied by distinct intracellular changes. These alterations extended to other B. thetaiotaomicron strains, but not Roseburia species. By re-analyzing an existing RNA-seq dataset, we could link the cell biological response of both species to different alterations in gene expression in stationary phase. Finally, coculture experiments revealed a significant impact of interspecies interactions on cell morphological and transcriptomic properties of both bacteria. Together, our work provides insight into the unexplored cell biology of representative human gut microbiome members and how this is shaped by nutrient fluctuations and the presence of other species.
Novel Insights into Systemic Hyaluronic Acid Therapy in Dogs with Osteoarthritis from an Exploratory Postmarketing Study: Clinical Improvements Linked to Biomarker Changes Jana Matonohová, Matěj Šimek, Vratislav Berka, Lucie Bystroňová, Iva Lžičařová, Daniela Rubanová, Lukáš Kubala, Vladimír Velebný, Kristina Nešporová Animals, 2025 This prospective, single-arm, exploratory postmarketing study preliminarily evaluated the clinical response and plasma biomarker changes in 18 client-owned dogs with naturally occurring osteoarthritis (OA) treated with sodium hyaluronate (Bonharen). Patients received intravenous injections of Bonharen Intravenous at a dose of 0.15 mL/kg (1.3–1.6 mg/kg hyaluronic acid once a week for consecutive five weeks). Clinical parameters (lameness, joint pain, mobility, swelling) were assessed weekly and two weeks after the final dose was given via standardized scoring. The plasma concentrations of selected inflammatory, cartilage-related, and oxidative stress biomarkers were measured before treatment and two weeks after the final dose. Clinical improvement in lameness and/or joint pain on palpation was observed in nearly half of the patients. No clinical deterioration was recorded at any time point. Physical activity increased in all patients with reduced baseline activity. Significant decreases in the plasma levels of prostaglandin E2, Δ17-6-keto prostaglandin F1α, malondialdehyde, and hyaluronan were detected, indicating reduced systemic inflammation and oxidative stress. In addition, an increase in plasma hydroxybutyrate and decrease in the collagen-breakdown marker prolyl-hydroxyproline were observed. No adverse effects were reported. These findings suggest that intravenous hyaluronic acid (Bonharen) may represent a safe and promising component to multimodal OA management in dogs and demonstrate the feasibility of integrating plasma biomarkers in canine OA studies.
Intravenous Administration of Hyaluronan in Veterinary Musculoskeletal Disorders: Therapeutic Applications and Mechanisms Daniela Rubanová, Kristina Nešporová, Lukáš Kubala International Journal of Veterinary Science and Medicine, 2025 Hyaluronan (HA), a natural polysaccharide, is a significant component in the extracellular matrix of connective tissues. Recently, HA has gained substantial attention in veterinary medicine due to its remarkable effects in promoting wound healing and supporting joint health. The route of administration plays a key role in the therapeutic potential of HA, as it affects the pharmacokinetics and bioavailability of HA within the body and diseased joints. Moreover, administration routes differ considerably regarding technical complexity and patient discomfort. This review summarizes recent studies exploring therapeutic applications of intravenous HA across various medical conditions, animal species, and clinical settings, emphasizing specific therapeutic effects, underlying mechanisms, clinical efficacy, and overall therapeutic potential, highlighting systemic effects beneficial for multijoint and systemic connective tissue conditions.
Alleviating UV-induced photoaging with oral hyaluronic acid: insights from a hairless mouse model Matěj Šimek, Romana Šínová, Vratislav Berka, Kateřina Lehká, Kristina Nešporová, Iva Dolečková, Martina Bajerová, Vojtěch Pavlík, Lukáš Kubala International Journal of Biological Macromolecules, 2025 Hyaluronic acid (HA) is known for its beneficial effects on skin hydration and elasticity. However, the mechanisms by which HA dietary intake attenuates pathological processes in photoaged skin are still unclear. In this study, oral administration of HA, alone or in combination with hydrolysed collagen, was investigated to alleviate UV-induced inflammation, extracellular matrix (ECM) degradation, and metabolic alterations in the skin of SKH-1 hairless mice repeatedly exposed to UVA and UVB radiation. Both supplements reduced UV-induced degradation of HA and collagen and decreased the inflammatory response in the skin. Interestingly, HA supplementation influenced cutaneous and plasma metabolism, elevating lipid catabolism and metabolic markers such as hydroxybutyrate and a-ketoglutarate. The addition of collagen did not provide any additional significant benefits over HA despite being administered at a 12-fold higher dose. These findings highlight superior role of HA as a nutritional supplement for skin health and provide insights into its potential mechanisms in addressing photoaged skin by modulating both skin and systemic metabolism.
Complex interplay between gene deletions and the environment uncovers cellular roles for genes of unknown function in Escherichia coli Kaat Sondervorst, Kristina Nesporova, Matthew Herdman, Bart Steemans, Joëlle Rosseels, Sander K. Govers Msystems, 2025 Phenotypic outcomes can be heavily affected by environmental factors. In this study, we exploited the previously observed nutrient dependency of cell biological phenotypic features captured by a cross-condition image-based profiling assay of Escherichia coli deletion strains to examine this in more detail. We identified several general principles, including the existence of a spectrum of deviating phenotypes across nutrient conditions (i.e., from nutrient- or feature-specific to pleiotropic phenotypic deviations), limited conservation of phenotypic deviations across nutrient conditions (i.e., limited phenotypic robustness), and a subset of nutrient-independent phenotypic deviations (indicative of consistent genetic determinants of specific phenotypic features). In a subsequent step, we used this cross-condition data set to identify five genes of unknown function ( yibN , yaaY , yfaQ , ybiJ , and yijD ), of which the deletion displayed either nutrient-independent phenotypic deviations or phenotypic similarities to genes of known function. These genes showed different levels of phylogenetic conservation, ranging from conserved across the tree of life ( yibN ) to only present in some genera of the Enterobacterales ( yaaY ). Analysis of the structural properties of the proteins encoded by these y-genes, identification of structural similarities to other proteins, and the examination of their subcellular localization yielded new insights into their contribution to E. coli cell morphogenesis, cell cycle progression, and cell growth. Together, our approach showcases how bacterial image-based profiling assays and data sets can serve as a gateway to reveal the function of uncharacterized proteins. IMPORTANCE Despite unprecedented access to genomic information, predicting phenotypes based on genotypes remains notoriously difficult. One major confounding factor is the environment and its ability to modulate phenotypic outcomes. Another fact is that a large fraction of protein-coding genes in bacterial genomes remain uncharacterized and have no known function. In this work, we use a large-scale cross-condition image-based profiling dataset to characterize nutrient-dependent phenotypic variability of Escherichia coli deletion strains and exploit it to provide insight into the cellular role of genes of unknown function. Through our analysis, we identified five genes of unknown function that we subsequently further characterized by examining their phylogenetic conservation, predicted structural properties and similarities, and their intracellular localization. Combined, this approach highlights the potential of cross-condition image-based profiling, which extracts many cell biological phenotypic readouts across multiple conditions, to better understand nutrient-dependent phenotypic variability and uncover protein function.
Optimizing Hyaluronan-Based Lubricants for Treating Thoracolumbar Fascia Pathologies: Insights from Tribological and Pharmacokinetic Studies Alexandra Streďanská, Matěj Šimek, Jana Matonohová, David Nečas, Martin Vrbka, Jakub Suchánek, Veronika Pavliňáková, Lucy Vojtová, Martin Hartl, Ivan Křupka, Kristina Nešporová Lubricants, 2025 In a world where the incidence of non-specific lower back pain (LBP) is steadily increasing, researchers are still searching for effective solutions for patients. Hyaluronic acid (HA) viscosupplementation is commonly used to restore lubrication in osteoarthritis (OA) and other medical applications, but its rapid metabolism limits efficacy. This study evaluates whether an HA derivative can replace native HA for the treatment of non-specific LBP while maintaining or enhancing its frictional properties and improving in vivo stability. Six HA-based lubricants, both native and derivatized, were tested in a tribological rabbit fascia model and a new synthetic model. Reduced HA derivative showed better tribological properties and longer in vivo residence time compared to native HA, as demonstrated in pharmacokinetic studies in rabbits. The 316 kDa HA and reduced HA exhibited the most stable tribological properties, which were influenced by their molecular weight and concentration. These findings suggest that both native and reduced HA are promising viscosupplements for intrafascial injection in the treatment of LBP, with reduced HA potentially enhancing effectiveness through a prolonged effect.
In situ delivery of synthetic preimplantation factor using aldehyde-modified hyaluronic acid hydrogel with immobilized complexes of chondroitin sulfate derivatives Tutut Habibah, Andrea Exnerová, Kristina Nešporová, Una FitzGerald, Abhay Pandit, Marek Ingr, Martin Pravda, Vladimír Velebný Carbohydrate Polymer Technologies and Applications, 2025 Synthetic preimplantation factor (SPIF) is a promising therapeutic agent for chronic inflammatory diseases like Multiple Sclerosis (MS), but frequent systemic dosing limits patient adherence and therapy efficacy. This study presents an injectable drug delivery system (DDS) using 2 % (w/v) aldehyde-modified hyaluronic acid (HAOX) and chondroitin sulfate (CSOX) to deliver 100 µg of Fluorescein isothiocyanate-modified SPIF (FITC-SPIF). The DDS utilizes electrostatic interactions between the negatively charged sulfate groups of CSOX and the positively charged amino acids of FITC-SPIF for effective entrapment. Key properties were analyzed, including moderate gelation time (193 s), swelling profile (18 %), injectability (27 G needle) and an established relevant release profile (20 μg/daily ± 3) via anomalous diffusion. Increasing CSOX concentration reduced initial burst release by 38 % (0.5 % CSOX) to 78 % (1 % CSOX), extending release time (T50 %) from 50 h (0.5 % CSOX) to 88 h (1 % CSOX). Additionally, the release of FITC-SPIF enhanced TGF-β secretion in THP-1 macrophages, indicating preserved biological activity. These findings highlight the tunable release and mechanical properties achieved by adjusting the HAOX:CSOX ratio, strategically aligning the DDS for targeted MS symptom management. This system potentially simplifies SPIF delivery and enhances therapeutic efficacy.
Injecting hyaluronan in the thoracolumbar fascia: A model study Kristina Nešporová, Jana Matonohová, Jarmila Husby, Evgeniy Toropitsyn, Lenka Divoká Stupecká, Aaron Husby, Tereza Suchánková Kleplová, Alexandra Streďanská, Matěj Šimek, David Nečas, Martin Vrbka, Robert Schleip, Vladimír Velebný International Journal of Biological Macromolecules, 2023
The Degradation of Hyaluronan in the Skin Petra Žádníková, Romana Šínová, Vojtěch Pavlík, Matěj Šimek, Barbora Šafránková, Martina Hermannová, Kristina Nešporová, Vladimír Velebný Biomolecules, 2022
Hyaluronan polymeric micelles for topical drug delivery Daniela Šmejkalová, Tomáš Muthný, Kristina Nešporová, Martina Hermannová, Eva Achbergerová, Gloria Huerta-Angeles, Marek Svoboda, Martin Čepa, Veronika Machalová, Dominika Luptáková, Vladimír Velebný Carbohydrate Polymers, 2017
Macrophage-based assays for the in vitro testing of the anti-inflammatory activity of mesenchymal stem cell-based products A Exnerová, S Seidlová, V Daňková, V Pavlík, K Nešporová bioRxiv, 2026.03. 04.709479 , 2026 2026
From ecology to evolution: plasmid- and colicin-mediated persistence of antibiotic-resistant Escherichia coli in gulls M Ruzickova, J Palkovicova, K Nesporova, M Rysava, R Pariza, S Krejci, ... Msystems 11 (2), e01663-25 , 2026 2026 Citations: 2
Characterization of a chronic UV-induced photoaging mouse model: insights into skin barrier dysfunction, extracellular matrix remodeling, and altered adipogenesis M Bajerova, R Sinova, M Simek, K Lehka, P Ovesna, M Cepa, I Doleckova, ... bioRxiv, 2026.04. 10.712660 , 2026 2026
Generation of Bioactive Stem Cell-Derived Secretome in 3D Bioreactor System: Towards Cell-Free Therapy in Veterinary Medicine V Daňková, A Exnerová, H Vágnerová, V Pavlík, K Nešporová Biomolecules 16 (1), 2 , 2025 2025 Citations: 3
Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis B Liu, K Sondervorst, K Nesporova, K Faust, SK Govers Communications Biology 8 (1), 1580 , 2025 2025
Novel Insights into Systemic Hyaluronic Acid Therapy in Dogs with Osteoarthritis from an Exploratory Postmarketing Study: Clinical Improvements Linked to Biomarker Changes J Matonohová, M Šimek, V Berka, L Bystroňová, I Lžičařová, D Rubanová, ... Animals 15 (21), 3140 , 2025 2025
Alleviating UV-induced Photoaging with oral hyaluronic acid: insights from a hairless mouse model M Šimek, R Šínová, V Berka, K Lehká, K Nešporová, I Dolečková, ... International Journal of Biological Macromolecules, 146340 , 2025 2025 Citations: 2
Large-scale genomic analysis reveals the distribution and diversity of type VI secretion systems in Escherichia coli K Nesporova, B Steemans, SK Govers Msystems 10 (7), e00105-25 , 2025 2025 Citations: 4
Complex interplay between gene deletions and the environment uncovers cellular roles for genes of unknown function in Escherichia coli K Sondervorst, K Nesporova, M Herdman, B Steemans, J Rosseels, ... Msystems 10 (7), e00206-25 , 2025 2025 Citations: 1
Cryopreservation medium comprising hyaluronic acid, use thereof and method of cryopreservation K Nesporova, T Prat, N Pilbauerova, J SUCHANEK, V Velebny US Patent App. 18/852,078 , 2025 2025
Uncovering Hyaluronidase Activity Using Hyaluronan Zymography in Biological Samples K Nešporová, K Lehká, P Musilová Zymography: Principles, Process, and Pitfalls, Volume 1, 143-150 , 2025 2025
Pharmacokinetics of the systemic application of hyaluronic acid for joint arthritis treatment M Šimek, D Rubanová, K Nešporová, S Skoroplyas, K Lehká, P Raptová, ... International Journal of Biological Macromolecules 307, 141937 , 2025 2025 Citations: 6
The absence of the ompA and type VI secretion system in the successful lineage of Acinetobacter baumannii ST19 A Valcek, DIC Strachinaru, K Nesporova, P Soentjens, A Stoefs, ... bioRxiv, 2025.04. 29.651177 , 2025 2025 Citations: 1
Optimizing Hyaluronan-Based Lubricants for Treating Thoracolumbar Fascia Pathologies: Insights from Tribological and Pharmacokinetic Studies A Streďanská, M Šimek, J Matonohová, D Nečas, M Vrbka, J Suchánek, ... Lubricants 13 (4), 184 , 2025 2025 Citations: 3
In situ delivery of synthetic preimplantation factor using aldehyde-modified hyaluronic acid hydrogel with immobilized complexes of chondroitin sulfate derivatives T Habibah, A Exnerová, K Nešporová, U FitzGerald, A Pandit, M Ingr, ... Carbohydrate Polymer Technologies and Applications 9, 100689 , 2025 2025
Hyaluronic acid derivative having a reduced polarity, method of preparation thereof, composition and use thereof R Buffa, V Svozil, T Klejch, M Hermannova, L Hejlova, H Vagnerova, ... US Patent App. 18/719,598 , 2025 2025
Intravenous Administration of Hyaluronan in Veterinary Musculoskeletal Disorders: Therapeutic Applications and Mechanisms D Rubanová, K Nešporová, L Kubala International Journal of Veterinary Science and Medicine 13 (1), 2 , 2025 2025
Enzymatically stable unsaturated hyaluronan-derived oligosaccharides with selective cytostatic properties T Klejch, R Buffa, M Šimek, K Nešporová, A Exnerová, J Bednařík, ... Carbohydrate Polymers 336, 122129 , 2024 2024 Citations: 7
Understanding frictional behavior in fascia tissues through tribological modeling and material substitution A Streďanská, D Nečas, M Vrbka, J Suchánek, J Matonohová, ... Journal of the Mechanical Behavior of Biomedical Materials 155, 106566 , 2024 2024 Citations: 3
Changing dynamics of antibiotic resistant Escherichia in Caspian gulls shows the importance of longitudinal environmental studies K Nesporova, M Ruzickova, H Tarabai, S Krejci, M Masarikova, J Lausova, ... Environment International 186, 108606 , 2024 2024 Citations: 10
MOST CITED SCHOLAR PUBLICATIONS
Hyaluronan polymeric micelles for topical drug delivery D Šmejkalová, T Muthný, K Nešporová, M Hermannová, E Achbergerová, ... Carbohydrate Polymers 156, 86-96 , 2017 2017 Citations: 130
Effects of wound dressings containing silver on skin and immune cells K Nešporová, V Pavlík, B Šafránková, H Vágnerová, P Odráška, O Žídek, ... Scientific reports 10 (1), 15216 , 2020 2020 Citations: 124
The degradation of hyaluronan in the skin P Žádníková, R Šínová, V Pavlík, M Šimek, B Šafránková, M Hermannová, ... Biomolecules 12 (2), 251 , 2022 2022 Citations: 110
Low molecular weight hyaluronan mediated CD44 dependent induction of IL-6 and chemokines in human dermal fibroblasts potentiates innate immune response L Vistejnova, B Safrankova, K Nesporova, R Slavkovsky, M Hermannova, ... Cytokine 70 (2), 97-103 , 2014 2014 Citations: 87
An effective translation: The development of hyaluronan-based medical products from the physicochemical, and preclinical aspects G Huerta-Ángeles, K Nešporová, G Ambrožová, L Kubala, V Velebný Frontiers in bioengineering and biotechnology 6, 62 , 2018 2018 Citations: 83
Hospital and community wastewater as a source of multidrug-resistant ESBL-producing Escherichia coli L Davidova-Gerzova, J Lausova, I Sukkar, K Nesporova, L Nechutna, ... Frontiers in Cellular and Infection Microbiology 13, 1184081 , 2023 2023 Citations: 75
Selective in vitro anticancer effect of superparamagnetic iron oxide nanoparticles loaded in hyaluronan polymeric micelles D Smejkalova, K Nesporova, G Huerta-Angeles, J Syrovátka, D Jirák, ... Biomacromolecules 15 (11), 4012-4020 , 2014 2014 Citations: 63
Phenotypic characterization and heterogeneity among modern clinical isolates of Acinetobacter baumannii A Valcek, C Philippe, C Whiteway, E Robino, K Nesporova, M Bové, ... Microbiology spectrum 11 (1), e03061-22 , 2023 2023 Citations: 60
Escherichia coli sequence type 457 is an emerging extended-spectrum-β-lactam-resistant lineage with reservoirs in wildlife and food-producing animals K Nesporova, ER Wyrsch, A Valcek, I Bitar, K Chaw, P Harris, J Hrabak, ... Antimicrobial Agents and Chemotherapy 65 (1), 10.1128/aac. 01118-20 , 2020 2020 Citations: 60
Urban wildlife crisis: Australian silver gull is a bystander host to widespread clinical antibiotic resistance ER Wyrsch, K Nesporova, H Tarabai, I Jamborova, I Bitar, I Literak, ... Msystems 7 (3), e00158-22 , 2022 2022 Citations: 51
Hyaluronan and its derivatives for ophthalmology: Recent advances and future perspectives GH Ángeles, K Nešporová Carbohydrate polymers 259, 117697 , 2021 2021 Citations: 51
In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway J Starigazdová, K Nešporová, M Čepa, R Šínová, D Šmejkalová, ... European Journal of Pharmaceutical Sciences 143, 105168 , 2020 2020 Citations: 46
Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid D Šmejkalová, K Nešporová, M Hermannová, G Huerta-Angeles, ... International Journal of Pharmaceutics 466 (1-2), 147-155 , 2014 2014 Citations: 45
Hyaluronan: A key player or just a bystander in skin photoaging? R Šínová, V Pavlík, M Ondrej, V Velebný, K Nešporová Experimental dermatology 31 (4), 442-458 , 2022 2022 Citations: 42
Molecular weight and gut microbiota determine the bioavailability of orally administered hyaluronic acid M Šimek, K Turková, M Schwarzer, K Nešporová, L Kubala, ... Carbohydrate Polymers 313, 120880 , 2023 2023 Citations: 40
Genomic analysis of a strain collection containing multidrug-, extensively drug-, pandrug-, and carbapenem-resistant modern clinical isolates of Acinetobacter baumannii A Valcek, K Nesporova, C Whiteway, T De Pooter, W De Coster, ... Antimicrobial Agents and Chemotherapy 66 (9), e00892-22 , 2022 2022 Citations: 36
Ultrasound-assisted production of highly-purified β-glucan schizophyllan and characterization of its immune properties D Smirnou, P Knotek, K Nesporova, D Smejkalova, V Pavlik, L Franke, ... Process Biochemistry 58, 313-319 , 2017 2017 Citations: 32
Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro B Šafránková, M Hermannová, K Nešporová, V Velebný, L Kubala International journal of biological macromolecules 107, 1-8 , 2018 2018 Citations: 27
Various conjugative plasmids carrying the mcr-5 gene in Escherichia coli isolates from healthy chickens in Paraguay K Nesporova, I Jamborova, A Valcek, M Medvecky, I Literak, M Dolejska Journal of Antimicrobial Chemotherapy 74 (11), 3394-3397 , 2019 2019 Citations: 26
Synthesis and study of branched hyaluronic acid with potential anticancer activity R Buffa, K Nešporová, I Basarabová, P Halamková, V Svozil, V Velebný Carbohydrate polymers 223, 115047 , 2019 2019 Citations: 26
