Mahmoud Ashry
Verified @yahoo.com
Zoology department, Faculty of Science
Al-Azhar University
Scopus Publications
Scopus Publications
Hussein A. Sultan, Mahmoud Ashry, Alaa M.H. El-Bitar, and Mohsen A. Moustafa
Medknow
Background and objective Natural products from various sources tend to be potential candidates for drug discovery. Natural and synthetic zeolites are potentially useful biopharmaceuticals and bio-tools due to their unique and outstanding physical and chemical properties; therefore, this study aimed to estimate the hepatorenal preventive and insulin release restoration efficiencies of zeolite (natural and synthetic) in STZ-induced diabetic rats. Materials and methods Post inductions of hyperglycemia with a single (ip) dose of STZ (55 mg/kg), the rats were arranged into four groups (8 rats each): (I) normal control group, (II) STZ-diabetic rats, (III) STZ-diabetic rats treated orally with natural zeolite (300 mg/kg/day), and IV) STZ-diabetic rats treated with synthetic zeolite (300 mg/kg/day). Results and conclusion After 6 weeks of treatment of diabetic animals, both zeolite types markedly exhibited antidiabetic, anti-inflammatory, hepato-nephroprotective, and antioxidative stress effects that were monitored from the significant reduction in glucose, ALAT, ASAT, urea, creatinine, MDA, and NO values concomitant with a significant rise in insulin, GSH, SOD and CAT values, close to the corresponding values of normal ones. Also, both zeolites succeeded to modulate STZ-induced histological distortion. In conclusion, both zeolites exhibited multi-health benefits with promising potential against STZ-induced diabetes. This effect may be attributed to the antioxidant and free radical scavenging mechanisms of zeolites that were evidenced by hepatorenal protective activities.
Esmail M. El-Fakharany, Yousra A. El-Maradny, Mahmoud Ashry, Khaled G. Abdel-Wahhab, Marwa E. Shaban, and Hamada El-Gendi
Springer Science and Business Media LLC
Esmail M. El-Fakharany, Yousra A. El-Maradny, Mahmoud Ashry, Khaled G. Abdel-Wahhab, Marwa E. Shabana, and Hamada El-Gendi
Springer Science and Business Media LLC
AbstractThe ethanolic extract of Coleus forskohlii Briq leaves was employed in the green synthesis of zinc nanoparticles (Zn-NPs) by an immediate, one-step, and cost-effective method in the present study. Zn-NPs were coated with purified bovine lactoferrin (LF) and characterized through different instrumental analysis. The biosynthesized Zn-NPs were white in color revealing oval to spherical-shaped particles with an average size of 77 ± 5.50 nm, whereas LF-coated Zn-NPs (LF-Zn-NPs) revealed a larger particles size of up to 98 ± 6.40 nm. The biosynthesized Zn-NPs and LF-Zn-NPs revealed negatively charged surfaces with zeta-potentials of – 20.25 ± 0.35 and – 44.3 ± 3.25 mV, respectively. Interestingly, the LF-Zn-NPs showed potent in vitro retardation for SARS-CoV-2 entry to host cells by binding to the ACE2-receptor and spike protein receptor binding domain at IC50 values of 59.66 and μg/mL, respectively. Additionally, the results indicated the ability of LF-Zn-NPs to inhibit SARS-CoV-2 replication by interfering with RNA-dependent RNA polymerase “RdRp” activity at IC50 of 49.23 μg/mL. In vivo, the LF-Zn-NPs displayed a protective and therapeutic activity against induced pulmonary fibrosis in Bleomycin-treated male albino rats owing to its anti-inflammatory, antioxidant, and significant reduction in CRP, LDH, ferritin, and D-dimer levels. The obtained findings offer a promising route for biosynthesized Zn-NPs and LF-Zn-NPs as promising candidates against COVID-19.
Khaled G. Abdel-Wahhab, Mahmoud Ashry, Laila K. Hassan, Mohamed H. A. Gadelmawla, Ghada M. Elqattan, Esmail M. El-Fakharany, and Fathia A. Mannaaa
Springer Science and Business Media LLC
Mahran Tony, Mahmoud Ashry, Mohammad M. A. Tanani, Abdelbaset M. A. Abdelreheem, and Mohammad R. K. Abdel-Samad
Springer Science and Business Media LLC
AbstractCarrion flies play a significant role in forensic entomotoxicology, where they are employed as alternative samples when traditional samples are unavailable. In situations of poisoned death, these toxins disrupt insect development and affect forensic entomology analyses. So, forensic entomotoxicologists must be aware of this impact. The present study aimed to determine the effects of aluminum phosphide (AlP) and cypermethrin (CP) on the biochemical parameters and antioxidant enzymes of the third instar of Chrysomya megacephala maggots. C. megacephala was reared on normal and poisoned rabbit carcasses with aluminum phosphide and cypermethrin. The third larval instar of C. megacephala was studied using by spectrophotometer for detection of total protein, (TP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione s-transferase (GST), catalase (CAT) and malondialdehyde (MDA). The results indicated to significantly decrease of TP, TAC, SOD, GST and CAT and increase of AST, ALT and MDA in the maggots reared on the poisoned carcasses with AlP or CP compared with control group. In conclusion, the tested insecticides brought about a decrease antioxidant enzyme activity and increase of MDA could be involved in free radicals in C. megacephala larvae leading to oxidative stress by these insecticidal components.
Hussien M. Abdel Salam, Salah M.E. Soliman, Mohamed H.A. Gadelmawla, Mahmoud Ashry Mahmoud Ashry, , , , and
Apex Publishing
Esmail M. El-Fakharany, Hamada El-Gendi, Yousra A. El-Maradny, Marwa M. Abu-Serie, Khaled G. Abdel-Wahhab, Marwa E. Shabana, and Mahmoud Ashry
Elsevier BV
Esmail M. El-Fakharany, Hamada El‑Gendi, Khaled Abdel-Wahhab, Marwa M. Abu-Serie, Doaa Galal El-Sahra, and Mahmoud Ashry
Informa UK Limited
Colorectal cancer (CRC) is a malignant tumor recognized as a major cause of morbidity and mortality throughout the world. Therefore, novel liposomes of oleic acid coated with camel α-lactalbumin (α-LA coated liposomes) were developed for their potential antitumor activity against CRC, both in vitro and in DMH-induced CRC-modeled animal. In vitro results indicated the high safety of α-LA coated liposomes towards normal human cells with potent antitumor activity against Caco-2 cells at an IC50 value of 57.01 ± 3.55 µM with selectivity index of 6.92 ± 0.48. This antitumor activity has been attributed to induction of the apoptotic mechanism, as demonstrated by nuclear condensation and arrest of Caco-2 cells in sub-G1 populations. α-LA coated liposomes also revealed a significant up-regulation of the p53 gene combined with a down-regulation of the Bcl2 gene. Moreover, in vivo results revealed that treatment of induced-CRC modeled animals with α-LA coated liposomes for six weeks markedly improved the CRC-disorders; this was achieved from the significant reduction in the values of AFP, CEA, CA19.9, TNF-α, IL-1β, MDA, and NO coupled with remarkable rise in SOD, GPx, GSH, CAT, and CD4+ levels. The histopathological findings asserted the therapeutic potential of α-LA coated liposomes in the treatment of CRC. Therefore, the present results proved the antitumor activity of α-LA coated liposomes against CRC through the restoration of impaired oxidative stress, improved immune response, and reduced inflammation.Communicated by Ramaswamy H. Sarma.
Esmail M. El-Fakharany, Mahmoud Ashry, Marwa M. Abu‑Serie, Khaled G. Abdel-Wahhab, Doaa Galal El-Sahra, and Hamada El‑Gendi
Informa UK Limited
Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent cancers, closely associated with cirrhosis and fibrosis. This study aimed to assess the antitumor activity of oleic acid-liposomes (uncoated liposomes) upon coating with albumin against HCC. The in vitro studies revealed the high safety of the prepared uncoated and albumin-coated liposomes to normal HFB-4 cells (EC100 of 35.57 ± 0.17 and 79.133 ± 2.92 µM, respectively) with significant anticancer activity against HepG-2 cells with IC50 of 56.29 ± 0.91 and 26.74 ± 0.64 µM, respectively. The albumin-coated liposomes revealed superior apoptosis induction potential (80.7%) with significant upregulation of p53 gene expression ( >7.0-folds), compared to OA. The in vivo study revealed that the administration of uncoated or albumin-coated liposomes (100 mg/kg) for six weeks markedly retarded the DENA-induced HCC in Wistar albino rates through regulating the liver enzymes, total bilirubin level, pro-inflammatory cytokines, and oxidative stress. Accordingly, the current study supports the in vitro and in vivo chemo-preventive feature of albumin-coated liposomes against HCC through modulation of apoptosis, improvement of the immune response, reduction of inflammation, and restoration of impaired oxidative stress, which is the first reported to the best of our knowledge.
Mahmoud Ashry, Hussam Askar, Manar M. Obiedallah, Ahmed Hussuin Elankily, Doaa Galal El-Sahra, Gamal Zayed, Mohamed A. Mustafa, Sawsan Abd El-Maksoud El-Shamy, Somaia A. Negm, Marwa A. El-Beltagy,et al.
Frontiers Media SA
The goal of the current study was to investigate the hormonal modulatory efficiency of hesperidin, through its regulatory potential of immunological, inflammatory, and/or antioxidant changes in on hyperthyroidism modeled adult female albino rats. Both normal and hyperthyroidism modeled rats (140-160g) were randomly divided into four groups (10 animals each) as follows: 1) healthy animals were daily ingested with saline for six weeks, and served as control group, 2) healthy animals were intraperitoneally injected with hesperidin (50 mg/kg/day) for a similar period, 3) hyperthyroidism-modeled animals without any treatment acted as positive control, and 4) hyperthyroidism-modeled animals were treated intraperitoneally with hesperidin for a similar period. The findings showed that hesperidin significantly modulated hyperthyroidism deteriorations, this was evidenced by a remarkable decline in serum T4, FT4, T3, FT3, TNF-α, IL1β-, IL4-, IL-6, and IL-10 levels, with a minor increase in TSH and significant raise in CD4+ level. Similarly, valuable improvement was observed in the oxidative status; serum SOD, GPx, CAT, and GSH levels were dramatically enhanced, associated with remarkable drop in MDA and NO levels. Also, hesperidin demonstrated nephro-hepatoprotective and anti-atherogenic potential, this was achieved from the notable reduction in ALAT and ASAT activities as well as urea, creatinine, cholesterol, and triglyceride close to the corresponding values of healthy group. These findings were supported by histological and immunohistochemical ones that showed a notable decrease in the expression of the calcitonin antibody. In conclusion, hesperidin possesses anti-hyperthyroidism, immunoinflammatory regulatory, and antioxidant activities that evidenced from the improvement of physio-architecture of the thyroid gland, reduction of inflammation and restoration of the impaired oxidative stress. This effect might be mechanized through immunological, inflammatory, apoptotic, and/or antioxidant modulatory pathways.
Esmail M. El-Fakharany, Mahmoud Ashry, Abd-Elaleem H. Abd-Elaleem, Mahmoud H. Romeih, Fatma Adly Morsy, Reem A. Shaban, and Khaled G. Abdel-Wahhab
Elsevier BV
Mahmoud Ashry, Hussam Askar, Abdallah Alian, Sabry A. H. Zidan, Doaa G. El-Sahra, Khaled G. Abdel-Wahhab, Sobhi F. Lamlom, Nader R. Abdelsalam, Mohamed E. Abd El-Hack, and Heba F. Gomaa
MDPI AG
One of the most common tumors to cause death worldwide is colon cancer. This study aims to investigate the antitumor potency of Litophyton sp. methanolic extract (LME) against DMH-induced colon cancer in adult male rats. Group (1) normal rats served as the control, group (2) normal rats were ip-injected with LME at a dose of 100 μg/kg/day, group (3) DMH-induced colon cancer animals, and group (4) colon cancer-modeled animals were treated with LME (100 μg/kg/day) for six weeks. The results revealed that injection of LME markedly regenerated the colon cancer pathophysiological disorders; this was monitored from the significant reduction in the values of serum biomarkers (CEA, CA19.9, AFP), cytokines (TNF-α and IL1β), and biochemical measurements (ALAT, ASAT, urea, creatinine, cholesterol, and triglycerides) matched significant increase of apoptotic biomarkers (CD4+); similarly, colon DNA fragmentation, MDA, and NO levels were down-regulated. In contrast, a remarkable upregulation in colon SOD, GPx, GSH, and CAT levels was noted. Moreover, the colon histopathological architecture showed obvious regenerations. Chromatography of LME resulted in the purification of two polyhydroxylated steroids (1 and 2) with potential cytotoxic activities. LME performed therapeutic potential colon tumorigenesis; therefore, LME may have a promising chemo-preventive feature against colon cancer, probably via enhancement of the apoptosis pathway, improvement of the immune response, reduction of inflammation, or/and restoration of the impaired oxidative stress.
Mahmoud Ashry, , Doaa Galal ELSahra, Khaled G. Abdel-Wahhab, Mahenor E. Abdelsalam, Hagar H. Mourad, Alaa M.H. El-Bitar, Heba F. Gomaa, , ,et al.
Negah Scientific Publisher
Background: Testicular dysfunction is one of the common side effects that results from the treatment with oxaliplatin® as a chemotherapy drug, and pharmaceutical search for agents to relieve the side effects are underway. The current study explored the possible ameliorative and regenerative effects of Costus ethanolic extract against testicular degeneration in male rats induced by oxaliplatin. Methods: Male Wistar albino rats weighing 150-200g were divided into four groups of 10 rats each as follows: group-1 control rats; group-2 rats treated orally with the extract at 50 mg/kg/day for six weeks; group-3 rats injected oxaliplatin intraperitoneally at 10 mg/kg/week for six successive weeks; group-4 rats were injected intraperitoneally with oxaliplatin at 10 mg/kg/week combined with a daily oral dose of the Costus extract for six weeks. Results: Data from the current study revealed that the extract lowered the toxic effect of oxaliplatin on the testicular tissue samples. This was evident by the significant rise in the serum of total & free testosterone and CD4 cells, and the levels of GSH, SOD and CAT activities in the testis coupled with a marked reduction of serum TNF-α and IL-1β and testis MDA, nitric oxide levels and DNA fragmentation. Also, the extract promoted the regeneration of the histopathological structure of the testis. Conclusion: This study proposes a novel therapeutic application for the Costus extract as a therapeutic agent against testicular toxicity induced by oxaliplatin treatment through its promising anti-inflammatory and antioxidant properties.
Khaled Abdel-Wahh, Mahmoud Ashry, Esmail El Fakhara, and Heba Gomaa
Science Alert
Background and Objective: Epilepsy is one of the normal neurological problems that came about because of strange electrical movements and prompt serious and far-reaching cell misfortune in the mind. This study aimed to investigate if a nano-Chitosan formulation loaded with bovine milk lactoperoxidase (LPO) and lactoferrin (LF) could prevent Lithium Chloride/Pilocarpine-induced epilepsy in rats or not. Materials and Methods: Adult male rats (200-250 g) were partitioned into four groups (8 animals each) as follows: Group (1) Normal rats served as control group and received saline orally, group (2) Normal rats ingested with a daily oral dose of LPO and LF-NPS formulation at 50 mg kg1, group (3) Pilocarpine-induced epileptic rats and group (4) Epilepsy-modeled rats were treated with LPO+LF NPs (50 mg/kg/day, orally) for 6 weeks. Results: The results revealed that the administration of LPO+LF-NPs markedly improved the induced-epilepsy disorders, this was monitored from the significant reduction in the values of caspase-3, TNF-α, IL-1β, CD4+, MDA and NO coupled with remarkable raise in AchE-ase, dopamine, serotonin, SOD and GPx, CAT and GSH values in both brain regions. Conclusion: This study supported the anti-epilepsy features of LPO+LF-NPS against Lithium Chloride/Pilocarpine-induced epilepsy in rats through the improvement of the immune response, reduction of inflammation and restoration of the impaired oxidative stress status.
Mahmoud Ashry, Abd ElraheemA Elshater, Hend Ahmed, KhaledG Abdel-Wahhab, FatmaAdly Morsy, and Rana Abd-Elstar
Medknow
Background and objective Cancer is a disease associated with an abnormal proliferation and growth of living cells; treatment with the anticancer therapy, Oxaliplatin (OXP) results in hepatotoxicity. The objective of this study was to evaluate the protective effect of costus ethanolic extract (CEE) against OXP-induced hepatotoxicity in a trail to improve its clinical use. Materials and methods Adult male Wistar rats (150–180 g body weight) were randomly divided into four groups (10 rats each): (a) healthy control group, (b) healthy rats treated orally with CEE (50 mg/kg/day), (c) rats injected intraperitoneally with OXP (10 mg/kg once/week), and (d) rats treated with CEE in combination with OXP. Results and conclusion After 6 weeks of treatment, the results revealed that CEE succeeded to decline OXP-induced hepatotoxicity; this was evidenced by the significant reduction in serum alanine aminotransferase (ALAT), aspartate aminotransferases (ASAT), GGT, alkaline phosphatase (ALP), total cholesterol, triglycerides, low dense lipoprotein-cholesterol (LDL-c), tumor necrosis factor-alpha (TNF-α), Interleukin -1 Beta (IL-1β), and alpha-fetoprotein values as well as hepatic malondialdehyde, nitric oxide, and DNA fragmentation coupled with a marked rise in serum CD4, albumin and high dense lipoprotein-cholesterol (HDL-c) levels, and hepatic glutathione, superoxide dismutase, and catalase values. These effects agonized the structural restoration of the histological picture of liver. It could be concluded that CEE succeeded to a great extent to counteract the oxidative stress of OXP and protect the liver against its toxic effects; CEE may be considered as a promising supplement-candidate for the protection of liver against the side effects of that anticancer drugs.
Heba F. Gomaa, Khaled G. Abdel-W, Mahmoud Ashry, and Doaa Galal EL-S
Science Alert
Background and Objective: The use of Doxorubicin® (Doxo) in the treatment of different tumours is restricted due to its cardiotoxicity. The objective of this study was to determine the protective effect of Balanites aegyptiaca extract against cardiotoxicity induced by Doxorubicin® in male rats. Materials and Methods: Adult male rats (140-160) were parted into 6 groups (10 animals each) as follows: Group (1) Normal rats the control, group (2) Rats were administered BAE (200 mg kg1) orally for 4 weeks, group (3) Rats were treated IP with the anticancer drug (Doxorubicin®) at the dose of (0.5 mg kg1) for 4 weeks, group (4) Administrated orally with BAE in combination with Doxo injection for 4 weeks, group (5) Rats orally with BAE before intoxication with Doxo for 4 weeks and finally group (6) Animals post-administration of BAE for 4 weeks after intoxication with Doxo. After 4 weeks of injections. Results: Revealed that BAE succeeded to decline the Doxorubicin cardiotoxicity, this was evidenced by the significant reduction of serum LDH, CK-MB, total cholesterol, triglycerides, HDL, TNF-α, IL-1β and IL-6 as well as cardiac MDA and nitric oxide levels coupled with marked improvement in serum LDL, PON1 as well as cardiac GSH, SOD and CAT. Moreover, the BAE induced prominent regeneration of the cardiac muscle. Conclusion: Balanites aegyptiaca extract may be a promising cardio-protector against Doxorubicin® toxicity mediated through their antioxidant and radical scavenging activities.
Rashad E.M. Said et al.
Egypts Presidential Specialized Council for Education and Scientific Research
Most environmental pollutants pose a threat to human and environmental health, as well as ecological integrity and productivity. As a result of increased human activity, chemical emissions have increased, adversely impacting both terrestrial and marine environments. Heavy metals are among the most hazardous pollutants (Huang et al., 2020; Khattab et al., 2021 ). Heavy metals are one of the most common pollutants because they can remain in the environment, accumulate in food chains, and cause toxicity in a variety of tissues and organs (Dural et al., 2006 ; Qiu 2015; Briffa et al., 2020). Contaminants in runoff from agricultural and industrial industries deteriorate the physical and chemical properties of water bodies, including salts, nutrients like phosphorus and nitrogen, and pesticide residues (Sharma and Bhattacharya, 2017). Heavy metals contaminants in water are mainly caused by industrial and agricultural discharges such as coal and oil combustion, phosphate fertilizers, sewage disposal plastics, mining activities and pesticides (Hamada et al., 2018; Zhong et al., 2018; Vergilio et al., 2020). In aquatic ecosystems, heavy metals have been found to accumulate in faunal organisms in general and in fish as they dominant the food web (Osman et al., 2012; Wariaghli et al., 2013; Authman et al., 2015). Fish is one of Egypt's most popular foods, whether farmed in aquatic systems or caught in natural ecosystems, so biomonitoring and evaluating ecosystem components are national responsibility. Metals occur in fish at much higher rates than in water and soil because fish are also at the top of the aquatic food chain. ARTICLE INFO ABSTRACT Article History: Received: April 3, 2021 Accepted: Aug. 27, 2021 Online: Sept. 9, 2021 _______________
KhaledG Abdel-Wahhab, HanaaF.A Elkareem, Abd El-NabyI Essawy, Mahmoud Ashry, and ManalA Badawi
Medknow
Background and objective Doxorubicin (Doxo) is an antibiotic that used in cancer treatment, with many complications like hepatotoxicity. The objective of this study was to explore the ameliorative effect of Balanites aegyptiaca aqueous extract (BAE) against Doxo-induced hepatotoxicity in male rats. Materials and methods Adult male Wistar rats (140–160 g) were randomly divided into six groups (10 animals each) as follows: group I, normal rats act as a control group; group II, rats ingested with BAE (200 mg/kg) for 4 weeks; group III, rats intoxicated (intraperitoneal) with Doxo (0.5 mg/kg) for 4 weeks; group IV, rats ingested with BAE in combination with Doxo injection for 4 weeks; group V, rats ingested with BAE for 4 weeks before Doxo injection for another 4 weeks; and group VI, rats ingested with BAE for 4 weeks after Doxo injection. Results The results revealed all BAE regimens succeeded to decrease the hepatotoxicity induced by Doxo. This was evidenced by the significant reduction of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin, urea, creatinine, tumor necrosis factor alpha, and interleukin-1beta levels, as well as hepatic malondialdehyde and nitric oxide levels. Moreover, a marked increase was observed in serum protein and albumin levels, as well as hepatic-reduced glutathione, superoxide dismutase, and catalase values. The obvious histopathological regenerations came in line with both serum and tissue biochemical findings. The Doxo-BAE combined regimen exhibited the highest potential of amelioration. Conclusion As a promising supplement, BAE exhibited hepatoprotective potential against Doxo-induced hepatic injuries; this could be mechanized through its antioxidant and radical scavenging exhibition of its bioactive constituents.
Khaled G. Abdel-Wahhab, Fathia A. Mannaa, Mahmoud Ashry, Doaa M. Khaled, Laila K. Hassan, and Heba F. Gomaa
Springer Science and Business Media LLC
Hussein A. Sultan, Mahmoud Ashry, Alaa M.H. El-Bi, Noha N. Yassen, Mahenor E. Abdelsa, and Mohssen A. Moustaf
Science Alert
Mahmoud Ashry, Doaa Galal El-S, Dalia A. Gaber, Mohamed A. Mustafa, and Khaled G. Abdel-Wahh
Science Alert
<b>Background and Objective:</b> Oxaliplatin<sup>®</sup> is an antineoplastic platinum-based compound; nephrotoxicity is one of its most serious side effects. This study aimed to explore the nephroprotective potential of Costus Ethanolic Extract (CEE) against Oxaliplatin<sup>®</sup>-induced nephrotoxicity. <b>Materials and Methods:</b> Adult male Wistar rats, weighting 140-160 g, were randomly divided into four groups: (1) Normal rats, (2) Rats ingested with CEE (67.08 mg kg<sup>1</sup> day<sup>1</sup>), (3) Rats injected (ip) with Oxaliplatin<sup>®</sup> (10 mg kg<sup>1</sup> week<sup>1</sup>) and (4) rats treated with CEE in combination Oxaliplatin<sup>®</sup> injection. <b>Results:</b> After six weeks of treatments, the results revealed that CEE ingestion along with Oxaliplatin<sup>®</sup> injection markedly minimized the Oxaliplatin<sup>®</sup>-induced renal deterioration; this was evidenced by the significant reduction in serum urea, creatinine, uric acid, Tumor Necrosis Factor Alpha (TNF-α), Interleukin 1Beta (IL<sup>1</sup>β) and Sodium ion (Na<sup>+</sup>) levels as well as kidney Malondialdehyde (MDA), Nitric Oxide (NO) and DNA fragmentation values. Controversially, a marked rise in serum Calcium, Potassium Ion (K<sup>+</sup>) and Cluster of Differentiation 4 (CD4) levels besides renal Glutathione (GSH), Catalase (CAT) and Superoxide Dismutase (SOD) values. Similarly, the histopathological findings confirmed the biochemical ones as the CEE restored the Oxaliplatin<sup>®</sup>-induced histological degenerations. <b>Conclusion:</b> In conclusion, CEE exhibited nephron-protection efficiency against Oxaliplatin<sup>®</sup>-induced nephrotoxicity; this promising effect may be achieved through the antioxidant and radical scavenging activities of its constituents.
Heba F. Gomaa, Khaled G. Abdel-W, Mahmoud Ashry, Laila K. Hassan, and Fathia A. Mannaa
Science Alert
BACKGROUND AND OBJECTIVE
Pyrethroidsare a group of synthetic pesticides similar to the natural pesticide pyrethrum, which is produced by chrysanthemum flowers. Bifenthrin is one of the pyrethroids that are widely used pesticide in households and to control crop vectors. The main goal of this work was to investigate the possible ameliorating effect of Costus Ethanolic Extract (CEE) against neurotoxicity induced by bifenthrin in adult-male rats.
MATERIALS AND METHODS
Rats were arranged randomly to 4 groups (8 rats each) as next. Group 1) control rats orally received 0.5 mL water for consecutive 30 days; group 2) healthy rats orally received CEE (200 mg kg) for consecutive thirty days; group 3) rats treated orally with 7 mg kg-1 day-1 bifenthrin for consecutive 30 days and group 4) included rats treated with bifenthrin for consecutive 30 days followed by administration with CEE another consecutive 30 days.
RESULTS
The results showed that CEE succeeded to decline the neurotoxicity-induced by bifenthrin; this was evidenced by the significant reduction in TNF-α, IL- 1β, MDA and nitric oxide levels in cortex, hippocampus and striatum concomitant with marked improvement in the values of GSH, dopamine, serotonin, AChE-ase, SOD, GPx and catalase that were diminished by bifenthrin intoxication. CEE improved also cognitive impairment and the deficits in motor coordination induced by bifenthrin.
CONCLUSION
CEE was found successful, to a great extent, to counteract the bifenthrin-induced brain oxidative stress and neurochemical deteriorations and possesses a protective potential against brain-induced neurotoxicity. Therefore, it may be a promising supplement for the amelioration of BF-neurotoxicity.
Mohamed A. Mustafa, Mahmoud Ashry, Heba H. Salama, Samy M. Abdelha, Laila K. Hassan, and Khaled G. Abdel-Wahh
Science Alert